Rh2DG642200

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000309
Physical Location & Seq
Reverse (-)
86911255 .. 86912382
1128 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG642200.1

Sequence Viewer

Length: 918 bp
ATGGCACCAAAATCGTATCTTTCTCTTTTCACAGTCACTCTCCTTCTCCTCCTCCTCACCCTCTTTTCTTTCCTCTCTAATGCAACGGCAAACTCATCTCCGTTTGAGGTCCACGAGCATCTTAAGGGTTGTCACAAAGGTGACAAGGTCAAGGGCATCAATGACCTCAAGAAGTACCTTCAAAAGTTCGGTTACTTAAATTATGAGAACCACAGGCATTCCAATGACGATGATTTTGACGAGCACTTGGAGGAAGCCGTCAAGACTTACCAGCTCAATTTCCACCTCAAGTCCACCGGAACATTGGACGACAAAACCCTATCAACGATGATGATGCCTCGTTGTGGTGTGCCTGATATCATCAATGGCACCACATCCATGCGATCAGCCCAGATAAAGCACCAGCACCGTGCAATTCATACCACTGTTCACTTCTCATTTCCTGATGGAAATCTAATATGGCCTTCTTCAAAATACCATCTAACTTACGGTTTTCTTGCCGGCACCCCATCAGAAGCCCAGGGCGCTGTCACACAGGCTTTCTCAACATGGGCTAAGAACACTCACTTCAAATTCAGCAAGGCTCAAAATTACCAGAGTGCGGATTTGAAGGTTAGCTTTCATAAGGGTGATCATGGAGACGGGAATTCATTTGATGGGCCTGGCGGACAGCTGGCCCATGCTGCTTACCCTACGGAGGGATCATTGCACTACGACGCAGATGAGACTTGGTCTGTAGGTGCCAAACCAGGTGCCATGGACATTGAGAGTGTTGGTTTGCATGAAATCGGGCACCTTCTGGGACTTGGACACAGCTCAGTGGAGGAAGCTATCATGTATCCAACCATCCCCGAAGGAGTGTATAAAAGTTTGCATGAGGACGATATTCAAGGAATAAAAGCTTTATACAACATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

305

Amino Acids

33.81

Weight (kDa)

6.13

Isoelectric Point (pI)

23.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 53 - 110 1.8e-11 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 157 - 303 1.4e-45 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 6 cut(s) 4, 368, 503, 740, 752, 792
AciI CCGC 2 cut(s) 602, 666
AclWI GGATC 1 cut(s) 709
AcsI RAATTY 2 cut(s) 572, 646
AfaI GTAC 1 cut(s) 176
AfiI CCNNNNNNNGG 4 cut(s) 344, 601, 697, 698
AflII CTTAAG 1 cut(s) 122
AgsI TTSAA 5 cut(s) 182, 471, 571, 610, 890
AjnI CCWGG 3 cut(s) 519, 661, 748
AleI CACNNNNGTG 1 cut(s) 138
AluBI AGCT 6 cut(s) 274, 618, 673, 816, 830, 902
AluI AGCT 6 cut(s) 274, 618, 673, 816, 830, 902
Alw21I GWGCWC 1 cut(s) 246
Alw26I GTCTC 2 cut(s) 633, 719
AlwI GGATC 1 cut(s) 709
AoxI GGCC 3 cut(s) 461, 659, 675
ApeKI GCWGC 1 cut(s) 683
ApoI RAATTY 2 cut(s) 572, 646
ArsI GACNNNNNNTTYG 2 cut(s) 218, 250
AspLEI GCGC 1 cut(s) 527
AspS9I GGNCC 3 cut(s) 109, 659, 676
AsuHPI GGTGA 3 cut(s) 49, 152, 641
AvaII GGWCC 1 cut(s) 109
BaeGI GKGCMC 1 cut(s) 795
BanI GGYRCC 6 cut(s) 4, 368, 503, 740, 752, 792
BauI CACGAG 1 cut(s) 113
Bbv12I GWGCWC 1 cut(s) 246
BbvI GCAGC 1 cut(s) 670
BccI CCATC 5 cut(s) 440, 486, 517, 650, 854
BceAI ACGGC 2 cut(s) 102, 242
BcgI CGANNNNNNTGC 3 cut(s) 28, 316, 350
BciT130I CCWGG 3 cut(s) 521, 663, 750
BciVI GTATCC 1 cut(s) 849
BclI TGATCA 1 cut(s) 631
BcoDI GTCTC 2 cut(s) 633, 719
BfmI CTRYAG 1 cut(s) 735
BfoI RGCGCY 1 cut(s) 528
BfrI CTTAAG 1 cut(s) 122
BfuI GTATCC 1 cut(s) 849
BisI GCNGC 1 cut(s) 684
BlsI GCNGC 1 cut(s) 685
Bme1390I CCNGG 3 cut(s) 521, 663, 750
Bme18I GGWCC 1 cut(s) 109
BmgT120I GGNCC 3 cut(s) 109, 659, 676
BmiI GGNNCC 6 cut(s) 6, 370, 505, 742, 754, 794
BmrFI CCNGG 3 cut(s) 521, 663, 750
BmsI GCATC 3 cut(s) 127, 165, 324
BpuEI CTTGAG 2 cut(s) 152, 272
BsaBI GATNNNNATC 1 cut(s) 450
BsaJI CCNNGG 3 cut(s) 519, 520, 756
BsaWI WCCGGW 1 cut(s) 296
Bsc4I CCNNNNNNNGG 4 cut(s) 344, 601, 697, 698
Bse118I RCCGGY 1 cut(s) 500
Bse3DI GCAATG 1 cut(s) 704
Bse8I GATNNNNATC 1 cut(s) 450
BseBI CCWGG 3 cut(s) 521, 663, 750
BseDI CCNNGG 3 cut(s) 519, 520, 756
BseGI GGATG 2 cut(s) 374, 846
BseJI GATNNNNATC 1 cut(s) 450
BseLI CCNNNNNNNGG 4 cut(s) 344, 601, 697, 698
BseMI GCAATG 1 cut(s) 704
BseMII CTCAG 1 cut(s) 831
BseRI GAGGAG 3 cut(s) 38, 41, 44
BseSI GKGCMC 1 cut(s) 795
BseXI GCAGC 1 cut(s) 670
BshFI GGCC 3 cut(s) 463, 661, 677
BshNI GGYRCC 6 cut(s) 4, 368, 503, 740, 752, 792
BsiHKAI GWGCWC 1 cut(s) 246
BsiSI CCGG 2 cut(s) 297, 501
BslFI GGGAC 1 cut(s) 816
BslI CCNNNNNNNGG 4 cut(s) 344, 601, 697, 698
BsmAI GTCTC 2 cut(s) 633, 719
BsmBI CGTCTC 1 cut(s) 633
BsmFI GGGAC 1 cut(s) 816
BsmI GAATGC 1 cut(s) 217
BsnI GGCC 3 cut(s) 463, 661, 677
Bsp1286I GDGCHC 2 cut(s) 246, 795
Bsp143I GATC 3 cut(s) 383, 631, 701
Bsp19I CCATGG 1 cut(s) 756
BspACI CCGC 2 cut(s) 602, 666
BspANI GGCC 3 cut(s) 463, 661, 677
BspCNI CTCAG 1 cut(s) 830
BspLI GGNNCC 6 cut(s) 6, 370, 505, 742, 754, 794
BspPI GGATC 1 cut(s) 709
BspT107I GGYRCC 6 cut(s) 4, 368, 503, 740, 752, 792
BspTI CTTAAG 1 cut(s) 122
BsrDI GCAATG 1 cut(s) 704
BsrFI RCCGGY 1 cut(s) 500
BssAI RCCGGY 1 cut(s) 500
BssECI CCNNGG 3 cut(s) 519, 520, 756
BssMI GATC 3 cut(s) 383, 631, 701
BssSI CACGAG 1 cut(s) 113
BssT1I CCWWGG 1 cut(s) 756
Bst2BI CACGAG 1 cut(s) 113
Bst2UI CCWGG 3 cut(s) 521, 663, 750
Bst4CI ACNGT 4 cut(s) 34, 410, 427, 491
BstAFI CTTAAG 1 cut(s) 122
BstC8I GCNNGC 2 cut(s) 502, 675
BstDEI CTNAG 2 cut(s) 555, 817
BstDSI CCRYGG 1 cut(s) 756
BstF5I GGATG 2 cut(s) 374, 846
BstH2I RGCGCY 1 cut(s) 528
BstHHI GCGC 1 cut(s) 527
BstKTI GATC 3 cut(s) 386, 634, 704
BstMAI GTCTC 2 cut(s) 633, 719
BstMBI GATC 3 cut(s) 383, 631, 701
BstMWI GCNNNNNNNGC 2 cut(s) 524, 683
BstNI CCWGG 3 cut(s) 521, 663, 750
BstSCI CCNGG 3 cut(s) 519, 661, 748
BstSFI CTRYAG 1 cut(s) 735
BstSLI GKGCMC 1 cut(s) 795
BstV1I GCAGC 1 cut(s) 670
BsuI GTATCC 1 cut(s) 849
BsuRI GGCC 3 cut(s) 463, 661, 677
BtgI CCRYGG 1 cut(s) 756
BtsCI GGATG 2 cut(s) 374, 846
BtsIMutI CAGTG 2 cut(s) 423, 825
Cac8I GCNNGC 2 cut(s) 502, 675
CfoI GCGC 1 cut(s) 527
Cfr10I RCCGGY 1 cut(s) 500
Cfr13I GGNCC 3 cut(s) 109, 659, 676
CseI GACGC 1 cut(s) 725
CsiI ACCWGGT 1 cut(s) 748
Csp6I GTAC 1 cut(s) 175
CviAII CATG 8 cut(s) 379, 549, 635, 680, 757, 782, 835, 875
CviQI GTAC 1 cut(s) 175
DdeI CTNAG 2 cut(s) 555, 817
DpnI GATC 3 cut(s) 385, 633, 703
DpnII GATC 3 cut(s) 383, 631, 701
EciI GGCGGA 1 cut(s) 681
Eco130I CCWWGG 1 cut(s) 756
Eco32I GATATC 1 cut(s) 358
Eco47I GGWCC 1 cut(s) 109
EcoRI GAATTC 1 cut(s) 646
EcoRII CCWGG 3 cut(s) 519, 661, 748
EcoRV GATATC 1 cut(s) 358
EcoT14I CCWWGG 1 cut(s) 756
ErhI CCWWGG 1 cut(s) 756
Esp3I CGTCTC 1 cut(s) 633
FaeI CATG 8 cut(s) 382, 552, 638, 683, 760, 785, 838, 878
FalI AAGNNNNNCTT 2 cut(s) 602, 634
FaqI GGGAC 1 cut(s) 816
FatI CATG 8 cut(s) 378, 548, 634, 679, 756, 781, 834, 874
FbaI TGATCA 1 cut(s) 631
Fnu4HI GCNGC 1 cut(s) 684
FokI GGATG 2 cut(s) 361, 833
Fsp4HI GCNGC 1 cut(s) 684
GlaI GCGC 1 cut(s) 526
GluI GCNGC 1 cut(s) 684
HaeII RGCGCY 1 cut(s) 528
HaeIII GGCC 3 cut(s) 463, 661, 677
HapII CCGG 2 cut(s) 297, 501
HgaI GACGC 1 cut(s) 725
HhaI GCGC 1 cut(s) 527
Hin1II CATG 8 cut(s) 382, 552, 638, 683, 760, 785, 838, 878
Hin6I GCGC 1 cut(s) 525
HinP1I GCGC 1 cut(s) 525
HindIII AAGCTT 1 cut(s) 900
HpaII CCGG 2 cut(s) 297, 501
HphI GGTGA 3 cut(s) 49, 152, 641
Hpy166II GTNNAC 3 cut(s) 112, 294, 430
Hpy188I TCNGA 1 cut(s) 514
Hpy188III TCNNGA 3 cut(s) 169, 262, 443
Hpy8I GTNNAC 3 cut(s) 112, 294, 430
Hpy99I CGWCG 1 cut(s) 719
HpyAV CCTTC 6 cut(s) 53, 188, 474, 604, 806, 848
HpyCH4III ACNGT 4 cut(s) 34, 410, 427, 491
HpyCH4V TGCA 5 cut(s) 83, 413, 709, 781, 874
HpyF10VI GCNNNNNNNGC 2 cut(s) 524, 683
HpyF3I CTNAG 2 cut(s) 555, 817
Hsp92II CATG 8 cut(s) 382, 552, 638, 683, 760, 785, 838, 878
HspAI GCGC 1 cut(s) 525
KroI GCCGGC 1 cut(s) 500
KroNI GCCGGC 1 cut(s) 502
Ksp22I TGATCA 1 cut(s) 631
Kzo9I GATC 3 cut(s) 383, 631, 701
Lsp1109I GCAGC 1 cut(s) 670
LweI GCATC 3 cut(s) 127, 165, 324
MabI ACCWGGT 1 cut(s) 748
MaeIII GTNAC 5 cut(s) 34, 131, 140, 191, 529
MalI GATC 3 cut(s) 385, 633, 703
MboI GATC 3 cut(s) 383, 631, 701
MboII GAAGA 1 cut(s) 459
MhlI GDGCHC 2 cut(s) 246, 795
MluCI AATT 6 cut(s) 199, 277, 414, 572, 589, 646
MmeI TCCRAC 1 cut(s) 866
MroNI GCCGGC 1 cut(s) 500
MseI TTAA 2 cut(s) 123, 197
MslI CAYNNNNRTG 4 cut(s) 138, 222, 377, 627
MspA1I CMGCKG 1 cut(s) 673
MspCI CTTAAG 1 cut(s) 122
MspI CCGG 2 cut(s) 297, 501
MspR9I CCNGG 3 cut(s) 521, 663, 750
Mva1269I GAATGC 1 cut(s) 217
MvaI CCWGG 3 cut(s) 521, 663, 750
MwoI GCNNNNNNNGC 2 cut(s) 524, 683
NaeI GCCGGC 1 cut(s) 502
NcoI CCATGG 1 cut(s) 756
NdeII GATC 3 cut(s) 383, 631, 701
NgoMIV GCCGGC 1 cut(s) 500
NlaIII CATG 8 cut(s) 382, 552, 638, 683, 760, 785, 838, 878
NlaIV GGNNCC 6 cut(s) 6, 370, 505, 742, 754, 794
NmuCI GTSAC 4 cut(s) 34, 131, 140, 529
OliI CACNNNNGTG 1 cut(s) 138
PasI CCCWGGG 1 cut(s) 520
PctI GAATGC 1 cut(s) 217
PdiI GCCGGC 1 cut(s) 502
PflFI GACNNNGTC 2 cut(s) 146, 730
PkrI GCNGC 1 cut(s) 685
Psp6I CCWGG 3 cut(s) 519, 661, 748
PspGI CCWGG 3 cut(s) 519, 661, 748
PspN4I GGNNCC 6 cut(s) 6, 370, 505, 742, 754, 794
PspPI GGNCC 3 cut(s) 109, 659, 676
PsyI GACNNNGTC 2 cut(s) 146, 730
PvuII CAGCTG 1 cut(s) 673
RsaI GTAC 1 cut(s) 176
RsaNI GTAC 1 cut(s) 175
RseI CAYNNNNRTG 4 cut(s) 138, 222, 377, 627
SaqAI TTAA 2 cut(s) 123, 197
SatI GCNGC 1 cut(s) 684
Sau3AI GATC 3 cut(s) 383, 631, 701
Sau96I GGNCC 3 cut(s) 109, 659, 676
ScrFI CCNGG 3 cut(s) 521, 663, 750
SduI GDGCHC 2 cut(s) 246, 795
SexAI ACCWGGT 1 cut(s) 748
SfaNI GCATC 3 cut(s) 127, 165, 324
SfcI CTRYAG 1 cut(s) 735
SinI GGWCC 1 cut(s) 109
SmiMI CAYNNNNRTG 4 cut(s) 138, 222, 377, 627
SmlI CTYRAG 3 cut(s) 122, 167, 287
SmoI CTYRAG 3 cut(s) 122, 167, 287
Sse9I AATT 6 cut(s) 199, 277, 414, 572, 589, 646
SsiI CCGC 2 cut(s) 602, 666
StyD4I CCNGG 3 cut(s) 519, 661, 748
StyI CCWWGG 1 cut(s) 756
TaaI ACNGT 4 cut(s) 34, 410, 427, 491
TasI AATT 6 cut(s) 199, 277, 414, 572, 589, 646
Tru1I TTAA 2 cut(s) 123, 197
Tru9I TTAA 2 cut(s) 123, 197
TscAI CASTG 2 cut(s) 430, 825
TseFI GTSAC 4 cut(s) 34, 131, 140, 529
TseI GCWGC 1 cut(s) 683
Tsp45I GTSAC 4 cut(s) 34, 131, 140, 529
TspDTI ATGAA 4 cut(s) 407, 611, 639, 798
TspGWI ACGGA 2 cut(s) 90, 710
TspRI CASTG 2 cut(s) 430, 825
Tth111I GACNNNGTC 2 cut(s) 146, 730
Vha464I CTTAAG 1 cut(s) 122
VpaK11BI GGWCC 1 cut(s) 109
XapI RAATTY 2 cut(s) 572, 646
XcmI CCANNNNNNNNNTGG 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.