Rh4CG107400

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000309
Physical Location & Seq
Forward (+)
20033216 .. 20034052
837 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG107400.1

Sequence Viewer

Length: 579 bp
ATGATGGCGCCCCGTTGCGGTGTGCCAGATCATATTGGCGTCCACAAAATCGACAATAGTAAAAAGGATCACATTAATGAAGTCTCTCACTATTCTTTCTTCAGCAAAAAGCAAAAATGGCCTCCTGCCAAGTGCCAGCTCACCTACGCTTTCGCTCCGGGCACTCCATCCGAAGCCAAGACTCCGGTTGCACAAGCTTTTCAAACGTGGGCGGCCAGCACTCACTTCAAGTTCAGACGACACAAAGCTTACAAGAAGGCTGACATAAAGATCAGTTTCGAACAAGGGGATCACGGAGATGGGAGCTCATTTGACGGCGCCGGTGGGATTCTAGCTCATGCTTATGCACCGACTTATGGAAAGCTCCACTACGACGCCGACGAGCCCTGGTCCGTCGGCGCCTTGGAAGGTTATTATGACTTGGAGACAGTGGCTTTGCATGAGATTGGTCACCTTCTCGGACTTGCTCATAGCTCGGTTGAGGGAGCTATCATGTACCCTTACATCTCTTCTGGAGTTACCAAGCTGGATTTGCATCATGACGATATTCACGGAATTAGAGCTTTATACAAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

192

Amino Acids

21.22

Weight (kDa)

6.94

Isoelectric Point (pI)

38.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M10 PF00413 40 - 190 1.6e-48 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 7, 317, 398
AciI CCGC 2 cut(s) 18, 212
AclWI GGATC 2 cut(s) 75, 297
AcoI YGGCCR 1 cut(s) 213
AcuI CTGAAG 1 cut(s) 85
AcyI GRCGYC 5 cut(s) 8, 39, 318, 375, 399
AfaI GTAC 1 cut(s) 497
AfiI CCNNNNNNNGG 2 cut(s) 17, 356
AgsI TTSAA 2 cut(s) 203, 229
AjnI CCWGG 1 cut(s) 386
Alw21I GWGCWC 1 cut(s) 308
Alw26I GTCTC 2 cut(s) 88, 419
AlwI GGATC 2 cut(s) 75, 297
AoxI GGCC 2 cut(s) 119, 213
ArsI GACNNNNNNTTYG 2 cut(s) 418, 450
AseI ATTAAT 1 cut(s) 75
AspLEI GCGC 3 cut(s) 10, 320, 401
AspS9I GGNCC 1 cut(s) 390
AsuC2I CCSGG 1 cut(s) 159
AsuHPI GGTGA 2 cut(s) 133, 443
AsuII TTCGAA 1 cut(s) 279
AvaII GGWCC 1 cut(s) 390
BaeGI GKGCMC 1 cut(s) 164
BanI GGYRCC 3 cut(s) 7, 317, 398
BanII GRGCYC 2 cut(s) 308, 387
Bbv12I GWGCWC 1 cut(s) 308
BccI CCATC 2 cut(s) 175, 293
BceAI ACGGC 1 cut(s) 331
BciT130I CCWGG 1 cut(s) 388
BcnI CCSGG 1 cut(s) 159
BcoDI GTCTC 2 cut(s) 88, 419
BfaI CTAG 1 cut(s) 332
BfoI RGCGCY 3 cut(s) 11, 321, 402
BisI GCNGC 1 cut(s) 213
BlsI GCNGC 1 cut(s) 214
Bme1390I CCNGG 2 cut(s) 159, 388
Bme18I GGWCC 1 cut(s) 390
BmgT120I GGNCC 1 cut(s) 390
BmiI GGNNCC 3 cut(s) 9, 319, 400
BmrFI CCNGG 2 cut(s) 159, 388
BmsI GCATC 1 cut(s) 544
BpmI CTGGAG 1 cut(s) 534
Bpu14I TTCGAA 1 cut(s) 279
BpuMI CCSGG 1 cut(s) 159
BsaBI GATNNNNATC 1 cut(s) 534
BsaHI GRCGYC 5 cut(s) 8, 39, 318, 375, 399
BsaJI CCNNGG 2 cut(s) 386, 402
BsaWI WCCGGW 1 cut(s) 184
Bsc4I CCNNNNNNNGG 2 cut(s) 17, 356
Bse118I RCCGGY 1 cut(s) 320
Bse8I GATNNNNATC 1 cut(s) 534
BseBI CCWGG 1 cut(s) 388
BseDI CCNNGG 2 cut(s) 386, 402
BseGI GGATG 1 cut(s) 167
BseJI GATNNNNATC 1 cut(s) 534
BseLI CCNNNNNNNGG 2 cut(s) 17, 356
BseSI GKGCMC 1 cut(s) 164
BshFI GGCC 2 cut(s) 121, 215
BshNI GGYRCC 3 cut(s) 7, 317, 398
BsiHKAI GWGCWC 1 cut(s) 308
BsiSI CCGG 3 cut(s) 158, 185, 321
BslI CCNNNNNNNGG 2 cut(s) 17, 356
BsmAI GTCTC 2 cut(s) 88, 419
BsnI GGCC 2 cut(s) 121, 215
Bsp119I TTCGAA 1 cut(s) 279
Bsp1286I GDGCHC 3 cut(s) 164, 308, 387
Bsp143I GATC 4 cut(s) 28, 67, 270, 289
BspACI CCGC 2 cut(s) 18, 212
BspANI GGCC 2 cut(s) 121, 215
BspHI TCATGA 1 cut(s) 538
BspLI GGNNCC 3 cut(s) 9, 319, 400
BspPI GGATC 2 cut(s) 75, 297
BspT104I TTCGAA 1 cut(s) 279
BspT107I GGYRCC 3 cut(s) 7, 317, 398
BsrFI RCCGGY 1 cut(s) 320
BssAI RCCGGY 1 cut(s) 320
BssECI CCNNGG 2 cut(s) 386, 402
BssMI GATC 4 cut(s) 28, 67, 270, 289
BssNI GRCGYC 5 cut(s) 8, 39, 318, 375, 399
BssT1I CCWWGG 1 cut(s) 402
Bst2UI CCWGG 1 cut(s) 388
Bst4CI ACNGT 1 cut(s) 430
Bst6I CTCTTC 1 cut(s) 514
BstACI GRCGYC 5 cut(s) 8, 39, 318, 375, 399
BstBI TTCGAA 1 cut(s) 279
BstC8I GCNNGC 2 cut(s) 137, 217
BstEII GGTNACC 1 cut(s) 449
BstF5I GGATG 1 cut(s) 167
BstH2I RGCGCY 3 cut(s) 11, 321, 402
BstHHI GCGC 3 cut(s) 10, 320, 401
BstKTI GATC 4 cut(s) 31, 70, 273, 292
BstMAI GTCTC 2 cut(s) 88, 419
BstMBI GATC 4 cut(s) 28, 67, 270, 289
BstMWI GCNNNNNNNGC 2 cut(s) 118, 532
BstNI CCWGG 1 cut(s) 388
BstPI GGTNACC 1 cut(s) 449
BstSCI CCNGG 2 cut(s) 157, 386
BstSLI GKGCMC 1 cut(s) 164
BsuRI GGCC 2 cut(s) 121, 215
BtsCI GGATG 1 cut(s) 167
BtsIMutI CAGTG 1 cut(s) 435
Cac8I GCNNGC 2 cut(s) 137, 217
CciI TCATGA 1 cut(s) 538
CfoI GCGC 3 cut(s) 10, 320, 401
Cfr10I RCCGGY 1 cut(s) 320
Cfr13I GGNCC 1 cut(s) 390
CseI GACGC 2 cut(s) 28, 383
Csp6I GTAC 1 cut(s) 496
CviAII CATG 4 cut(s) 338, 440, 493, 539
CviQI GTAC 1 cut(s) 496
DinI GGCGCC 3 cut(s) 9, 319, 400
DpnI GATC 4 cut(s) 30, 69, 272, 291
DpnII GATC 4 cut(s) 28, 67, 270, 289
EaeI YGGCCR 1 cut(s) 213
Eam1104I CTCTTC 1 cut(s) 514
EarI CTCTTC 1 cut(s) 514
Ecl136II GAGCTC 1 cut(s) 306
Eco130I CCWWGG 1 cut(s) 402
Eco24I GRGCYC 2 cut(s) 308, 387
Eco47I GGWCC 1 cut(s) 390
Eco53kI GAGCTC 1 cut(s) 306
Eco57I CTGAAG 1 cut(s) 85
Eco91I GGTNACC 1 cut(s) 449
EcoICRI GAGCTC 1 cut(s) 306
EcoO65I GGTNACC 1 cut(s) 449
EcoRII CCWGG 1 cut(s) 386
EcoT14I CCWWGG 1 cut(s) 402
EcoT38I GRGCYC 2 cut(s) 308, 387
EgeI GGCGCC 3 cut(s) 9, 319, 400
EheI GGCGCC 3 cut(s) 9, 319, 400
ErhI CCWWGG 1 cut(s) 402
FaeI CATG 4 cut(s) 341, 443, 496, 542
FatI CATG 4 cut(s) 337, 439, 492, 538
Fnu4HI GCNGC 1 cut(s) 213
FokI GGATG 1 cut(s) 154
FriOI GRGCYC 2 cut(s) 308, 387
Fsp4HI GCNGC 1 cut(s) 213
FspBI CTAG 1 cut(s) 332
GlaI GCGC 3 cut(s) 9, 319, 400
GluI GCNGC 1 cut(s) 213
GsuI CTGGAG 1 cut(s) 534
HaeII RGCGCY 3 cut(s) 11, 321, 402
HaeIII GGCC 2 cut(s) 121, 215
HapII CCGG 3 cut(s) 158, 185, 321
HgaI GACGC 2 cut(s) 28, 383
HhaI GCGC 3 cut(s) 10, 320, 401
Hin1I GRCGYC 5 cut(s) 8, 39, 318, 375, 399
Hin1II CATG 4 cut(s) 341, 443, 496, 542
Hin6I GCGC 3 cut(s) 8, 318, 399
HinP1I GCGC 3 cut(s) 8, 318, 399
HindIII AAGCTT 2 cut(s) 195, 246
HinfI GANTC 2 cut(s) 181, 328
HpaII CCGG 3 cut(s) 158, 185, 321
HphI GGTGA 2 cut(s) 133, 443
Hpy166II GTNNAC 1 cut(s) 43
Hpy188I TCNGA 3 cut(s) 172, 236, 461
Hpy188III TCNNGA 3 cut(s) 513, 539, 576
Hpy8I GTNNAC 1 cut(s) 43
Hpy99I CGWCG 3 cut(s) 377, 383, 398
HpyAV CCTTC 3 cut(s) 250, 401, 464
HpyCH4III ACNGT 1 cut(s) 430
HpyCH4IV ACGT 1 cut(s) 206
HpyCH4V TGCA 4 cut(s) 191, 347, 439, 535
HpyF10VI GCNNNNNNNGC 2 cut(s) 118, 532
HpySE526I ACGT 1 cut(s) 206
Hsp92I GRCGYC 5 cut(s) 8, 39, 318, 375, 399
Hsp92II CATG 4 cut(s) 341, 443, 496, 542
HspAI GCGC 3 cut(s) 8, 318, 399
KasI GGCGCC 3 cut(s) 7, 317, 398
Kzo9I GATC 4 cut(s) 28, 67, 270, 289
LmnI GCTCC 4 cut(s) 160, 303, 369, 485
LweI GCATC 1 cut(s) 544
MaeI CTAG 1 cut(s) 332
MaeII ACGT 1 cut(s) 206
MaeIII GTNAC 2 cut(s) 449, 517
MalI GATC 4 cut(s) 30, 69, 272, 291
MboI GATC 4 cut(s) 28, 67, 270, 289
MboII GAAGA 2 cut(s) 91, 501
MhlI GDGCHC 3 cut(s) 164, 308, 387
MluCI AATT 1 cut(s) 555
Mly113I GGCGCC 3 cut(s) 8, 318, 399
MlyI GAGTC 1 cut(s) 175
MnlI CCTC 2 cut(s) 132, 475
MseI TTAA 1 cut(s) 75
MslI CAYNNNNRTG 3 cut(s) 75, 297, 342
MspI CCGG 3 cut(s) 158, 185, 321
MspR9I CCNGG 2 cut(s) 159, 388
MvaI CCWGG 1 cut(s) 388
MwoI GCNNNNNNNGC 2 cut(s) 118, 532
NarI GGCGCC 3 cut(s) 8, 318, 399
NciI CCSGG 1 cut(s) 159
NdeII GATC 4 cut(s) 28, 67, 270, 289
NlaIII CATG 4 cut(s) 341, 443, 496, 542
NlaIV GGNNCC 3 cut(s) 9, 319, 400
NmuCI GTSAC 1 cut(s) 449
NspV TTCGAA 1 cut(s) 279
PagI TCATGA 1 cut(s) 538
PcsI WCGNNNNNNNCGW 1 cut(s) 378
PfeI GAWTC 1 cut(s) 328
PkrI GCNGC 1 cut(s) 214
PleI GAGTC 1 cut(s) 175
PluTI GGCGCC 3 cut(s) 11, 321, 402
PpsI GAGTC 1 cut(s) 175
PshBI ATTAAT 1 cut(s) 75
Psp124BI GAGCTC 1 cut(s) 308
Psp6I CCWGG 1 cut(s) 386
PspEI GGTNACC 1 cut(s) 449
PspGI CCWGG 1 cut(s) 386
PspN4I GGNNCC 3 cut(s) 9, 319, 400
PspPI GGNCC 1 cut(s) 390
RsaI GTAC 1 cut(s) 497
RsaNI GTAC 1 cut(s) 496
RseI CAYNNNNRTG 3 cut(s) 75, 297, 342
SacI GAGCTC 1 cut(s) 308
SaqAI TTAA 1 cut(s) 75
SatI GCNGC 1 cut(s) 213
Sau3AI GATC 4 cut(s) 28, 67, 270, 289
Sau96I GGNCC 1 cut(s) 390
SchI GAGTC 1 cut(s) 175
ScrFI CCNGG 2 cut(s) 159, 388
SduI GDGCHC 3 cut(s) 164, 308, 387
SfaNI GCATC 1 cut(s) 544
SfoI GGCGCC 3 cut(s) 9, 319, 400
SfuI TTCGAA 1 cut(s) 279
SgrAI CRCCGGYG 1 cut(s) 320
SinI GGWCC 1 cut(s) 390
SmiMI CAYNNNNRTG 3 cut(s) 75, 297, 342
Sse9I AATT 1 cut(s) 555
SsiI CCGC 2 cut(s) 18, 212
SspDI GGCGCC 3 cut(s) 7, 317, 398
SspMI CTAG 1 cut(s) 332
SstI GAGCTC 1 cut(s) 308
StyD4I CCNGG 2 cut(s) 157, 386
StyI CCWWGG 1 cut(s) 402
TaaI ACNGT 1 cut(s) 430
TaiI ACGT 1 cut(s) 209
TaqI TCGA 2 cut(s) 51, 279
TasI AATT 1 cut(s) 555
TauI GCSGC 1 cut(s) 215
TfiI GAWTC 1 cut(s) 328
Tru1I TTAA 1 cut(s) 75
Tru9I TTAA 1 cut(s) 75
TscAI CASTG 1 cut(s) 435
TseFI GTSAC 1 cut(s) 449
Tsp45I GTSAC 1 cut(s) 449
TspDTI ATGAA 1 cut(s) 93
TspGWI ACGGA 3 cut(s) 309, 382, 567
TspRI CASTG 1 cut(s) 435
VpaK11BI GGWCC 1 cut(s) 390
VspI ATTAAT 1 cut(s) 75
XspI CTAG 1 cut(s) 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.