Rh4AG098000

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000309
Physical Location & Seq
Forward (+)
21085435 .. 21089497
4063 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG098000.1

Sequence Viewer

Length: 762 bp
ATGGCAGAAACGTTCTTATTCAAATACATATTTATCCCGGGAATATTTTCAGGCACGTGCCTGCATGGGAAATCCCAGAGTCACACAAACTCATCATCATCGCCATTCGAGTTTCTGGAGCATCTCAAGGGGTCTCACAAGGGTGATCACATTCAAGGCATCCACGACCTCAAACTCTACCTTGAAAAATTCGGTTACTTAAGCTACGGAAACGACAAGAACCACACTCATGCTGACGATGACGATTTCGACGACGTCGTTGAGTCGGCCGTCAAGGACTACCAGCACTACTACCACCTCAGAGCCACCGGAACCCTTGACGGCAAAACCGTTTCCAAGATGATGGCGCCCCGTTGCGGTGTGCCAGATCATATTGGCGTCCACAAAATCGACAATAGTAAAAAGGATCACATTAATGAAGTCTCTCACTATTCTTTCTTCAGCAAAAAGCAAAAATGGCCTCCTGCCAAGTGCCAGCTCACCTACGCTTTCGCTCCGGGCACTCCATCCGAAGCCAAGACTCCGGTTGCACAAGCTTTTCAAACGTGGGCGGCCAGCACTCACTTCAAGTTCAGACGACACAAAGCTTACAAGAAGGCTGACATAAAGATCAGTTTCGAACAAGGGGATCACGGAGATGGGAGCTCATTTGACGGCGCCGGTGGGATTCTAGCTCATGCTTATGCACCGACTTATGGAAAGCTCCACTACGACGCCGACGAGCCCTGGTCCGTCGGCGCCTTGGAAGAAACGGATTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

253

Amino Acids

28.38

Weight (kDa)

6.54

Isoelectric Point (pI)

39.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 54 - 114 3.3e-10 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 153 - 247 1.7e-24 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 258
AccB1I GGYRCC 3 cut(s) 346, 656, 737
AciI CCGC 2 cut(s) 357, 551
AclI AACGTT 1 cut(s) 11
AclWI GGATC 2 cut(s) 414, 636
AcoI YGGCCR 2 cut(s) 267, 552
AcsI RAATTY 1 cut(s) 188
AcuI CTGAAG 1 cut(s) 424
AcvI CACGTG 1 cut(s) 57
AcyI GRCGYC 6 cut(s) 255, 347, 378, 657, 714, 738
AfiI CCNNNNNNNGG 2 cut(s) 356, 695
AflII CTTAAG 1 cut(s) 199
AgsI TTSAA 5 cut(s) 22, 155, 185, 542, 568
AjnI CCWGG 1 cut(s) 725
AleI CACNNNNGTG 1 cut(s) 141
AluBI AGCT 7 cut(s) 204, 478, 536, 587, 645, 674, 703
AluI AGCT 7 cut(s) 204, 478, 536, 587, 645, 674, 703
Alw21I GWGCWC 1 cut(s) 647
Alw26I GTCTC 2 cut(s) 138, 427
AlwI GGATC 2 cut(s) 414, 636
Ama87I CYCGRG 1 cut(s) 37
AoxI GGCC 3 cut(s) 267, 458, 552
ApoI RAATTY 1 cut(s) 188
AseI ATTAAT 1 cut(s) 414
Asp700I GAANNNNTTC 1 cut(s) 46
AspLEI GCGC 3 cut(s) 349, 659, 740
AspS9I GGNCC 1 cut(s) 729
AsuC2I CCSGG 3 cut(s) 38, 39, 498
AsuHPI GGTGA 2 cut(s) 155, 472
AsuII TTCGAA 1 cut(s) 618
AvaI CYCGRG 1 cut(s) 37
AvaII GGWCC 1 cut(s) 729
BaeGI GKGCMC 1 cut(s) 503
BanI GGYRCC 3 cut(s) 346, 656, 737
BanII GRGCYC 2 cut(s) 647, 726
BbrPI CACGTG 1 cut(s) 57
Bbv12I GWGCWC 1 cut(s) 647
BccI CCATC 3 cut(s) 337, 514, 632
BceAI ACGGC 3 cut(s) 254, 337, 670
BciT130I CCWGG 1 cut(s) 727
BclI TGATCA 1 cut(s) 145
BcnI CCSGG 3 cut(s) 38, 39, 498
BcoDI GTCTC 2 cut(s) 138, 427
BfaI CTAG 1 cut(s) 671
BfoI RGCGCY 3 cut(s) 350, 660, 741
BfrI CTTAAG 1 cut(s) 199
BisI GCNGC 1 cut(s) 552
BlsI GCNGC 1 cut(s) 553
Bme1390I CCNGG 4 cut(s) 38, 39, 498, 727
Bme18I GGWCC 1 cut(s) 729
BmeT110I CYCGRG 1 cut(s) 37
BmgT120I GGNCC 1 cut(s) 729
BmiI GGNNCC 4 cut(s) 313, 348, 658, 739
BmrFI CCNGG 4 cut(s) 38, 39, 498, 727
BmsI GCATC 2 cut(s) 130, 168
BpmI CTGGAG 1 cut(s) 137
Bpu14I TTCGAA 1 cut(s) 618
BpuEI CTTGAG 1 cut(s) 110
BpuMI CCSGG 3 cut(s) 38, 39, 498
BsaAI YACGTR 1 cut(s) 57
BsaHI GRCGYC 6 cut(s) 255, 347, 378, 657, 714, 738
BsaI GGTCTC 1 cut(s) 138
BsaJI CCNNGG 3 cut(s) 37, 725, 741
BsaWI WCCGGW 2 cut(s) 308, 523
Bsc4I CCNNNNNNNGG 2 cut(s) 356, 695
Bse118I RCCGGY 1 cut(s) 659
BseBI CCWGG 1 cut(s) 727
BseDI CCNNGG 3 cut(s) 37, 725, 741
BseGI GGATG 2 cut(s) 159, 506
BseLI CCNNNNNNNGG 2 cut(s) 356, 695
BseMII CTCAG 1 cut(s) 313
BseSI GKGCMC 1 cut(s) 503
BseX3I CGGCCG 1 cut(s) 267
Bsh1285I CGRYCG 1 cut(s) 270
BshFI GGCC 3 cut(s) 269, 460, 554
BshNI GGYRCC 3 cut(s) 346, 656, 737
BsiEI CGRYCG 1 cut(s) 270
BsiHKAI GWGCWC 1 cut(s) 647
BsiHKCI CYCGRG 1 cut(s) 37
BsiSI CCGG 5 cut(s) 38, 309, 497, 524, 660
BslI CCNNNNNNNGG 2 cut(s) 356, 695
BsmAI GTCTC 2 cut(s) 138, 427
BsnI GGCC 3 cut(s) 269, 460, 554
Bso31I GGTCTC 1 cut(s) 138
BsoBI CYCGRG 1 cut(s) 37
Bsp119I TTCGAA 1 cut(s) 618
Bsp1286I GDGCHC 3 cut(s) 503, 647, 726
Bsp143I GATC 5 cut(s) 145, 367, 406, 609, 628
BspACI CCGC 2 cut(s) 357, 551
BspANI GGCC 3 cut(s) 269, 460, 554
BspCNI CTCAG 1 cut(s) 312
BspLI GGNNCC 4 cut(s) 313, 348, 658, 739
BspPI GGATC 2 cut(s) 414, 636
BspT104I TTCGAA 1 cut(s) 618
BspT107I GGYRCC 3 cut(s) 346, 656, 737
BspTI CTTAAG 1 cut(s) 199
BspTNI GGTCTC 1 cut(s) 138
BsrFI RCCGGY 1 cut(s) 659
BssAI RCCGGY 1 cut(s) 659
BssECI CCNNGG 3 cut(s) 37, 725, 741
BssMI GATC 5 cut(s) 145, 367, 406, 609, 628
BssNI GRCGYC 6 cut(s) 255, 347, 378, 657, 714, 738
BssT1I CCWWGG 1 cut(s) 741
Bst2UI CCWGG 1 cut(s) 727
Bst4CI ACNGT 1 cut(s) 331
BstACI GRCGYC 6 cut(s) 255, 347, 378, 657, 714, 738
BstAFI CTTAAG 1 cut(s) 199
BstBAI YACGTR 1 cut(s) 57
BstBI TTCGAA 1 cut(s) 618
BstC8I GCNNGC 3 cut(s) 62, 476, 556
BstDEI CTNAG 1 cut(s) 299
BstF5I GGATG 2 cut(s) 159, 506
BstH2I RGCGCY 3 cut(s) 350, 660, 741
BstHHI GCGC 3 cut(s) 349, 659, 740
BstKTI GATC 5 cut(s) 148, 370, 409, 612, 631
BstMAI GTCTC 2 cut(s) 138, 427
BstMBI GATC 5 cut(s) 145, 367, 406, 609, 628
BstMCI CGRYCG 1 cut(s) 270
BstMWI GCNNNNNNNGC 1 cut(s) 457
BstNI CCWGG 1 cut(s) 727
BstSCI CCNGG 4 cut(s) 36, 37, 496, 725
BstSLI GKGCMC 1 cut(s) 503
BstXI CCANNNNNNTGG 1 cut(s) 343
BstZI CGGCCG 1 cut(s) 267
BsuRI GGCC 3 cut(s) 269, 460, 554
BtgZI GCGATG 1 cut(s) 84
BtsCI GGATG 2 cut(s) 159, 506
Cac8I GCNNGC 3 cut(s) 62, 476, 556
CfoI GCGC 3 cut(s) 349, 659, 740
Cfr10I RCCGGY 1 cut(s) 659
Cfr13I GGNCC 1 cut(s) 729
Cfr9I CCCGGG 1 cut(s) 37
CseI GACGC 2 cut(s) 367, 722
CviAII CATG 3 cut(s) 65, 230, 677
DdeI CTNAG 1 cut(s) 299
DinI GGCGCC 3 cut(s) 348, 658, 739
DpnI GATC 5 cut(s) 147, 369, 408, 611, 630
DpnII GATC 5 cut(s) 145, 367, 406, 609, 628
EaeI YGGCCR 2 cut(s) 267, 552
EagI CGGCCG 1 cut(s) 267
Ecl136II GAGCTC 1 cut(s) 645
EclXI CGGCCG 1 cut(s) 267
Eco130I CCWWGG 1 cut(s) 741
Eco24I GRGCYC 2 cut(s) 647, 726
Eco31I GGTCTC 1 cut(s) 138
Eco47I GGWCC 1 cut(s) 729
Eco52I CGGCCG 1 cut(s) 267
Eco53kI GAGCTC 1 cut(s) 645
Eco57I CTGAAG 1 cut(s) 424
Eco72I CACGTG 1 cut(s) 57
Eco88I CYCGRG 1 cut(s) 37
EcoICRI GAGCTC 1 cut(s) 645
EcoRII CCWGG 1 cut(s) 725
EcoT14I CCWWGG 1 cut(s) 741
EcoT38I GRGCYC 2 cut(s) 647, 726
EgeI GGCGCC 3 cut(s) 348, 658, 739
EheI GGCGCC 3 cut(s) 348, 658, 739
ErhI CCWWGG 1 cut(s) 741
FaeI CATG 3 cut(s) 68, 233, 680
FaiI YATR 8 cut(s) 29, 66, 231, 372, 605, 678, 684, 696
FatI CATG 3 cut(s) 64, 229, 676
FbaI TGATCA 1 cut(s) 145
Fnu4HI GCNGC 1 cut(s) 552
FokI GGATG 2 cut(s) 146, 493
FriOI GRGCYC 2 cut(s) 647, 726
Fsp4HI GCNGC 1 cut(s) 552
FspBI CTAG 1 cut(s) 671
GlaI GCGC 3 cut(s) 348, 658, 739
GluI GCNGC 1 cut(s) 552
GsuI CTGGAG 1 cut(s) 137
HaeII RGCGCY 3 cut(s) 350, 660, 741
HaeIII GGCC 3 cut(s) 269, 460, 554
HapII CCGG 5 cut(s) 38, 309, 497, 524, 660
HgaI GACGC 2 cut(s) 367, 722
HhaI GCGC 3 cut(s) 349, 659, 740
Hin1I GRCGYC 6 cut(s) 255, 347, 378, 657, 714, 738
Hin1II CATG 3 cut(s) 68, 233, 680
Hin6I GCGC 3 cut(s) 347, 657, 738
HinP1I GCGC 3 cut(s) 347, 657, 738
HindIII AAGCTT 2 cut(s) 534, 585
HinfI GANTC 4 cut(s) 79, 263, 520, 667
HpaII CCGG 5 cut(s) 38, 309, 497, 524, 660
HphI GGTGA 2 cut(s) 155, 472
Hpy166II GTNNAC 1 cut(s) 382
Hpy188I TCNGA 3 cut(s) 302, 511, 575
Hpy188III TCNNGA 1 cut(s) 116
Hpy8I GTNNAC 1 cut(s) 382
Hpy99I CGWCG 6 cut(s) 254, 257, 260, 716, 722, 737
HpyAV CCTTC 1 cut(s) 589
HpyCH4III ACNGT 1 cut(s) 331
HpyCH4IV ACGT 4 cut(s) 11, 56, 255, 545
HpyCH4V TGCA 3 cut(s) 64, 530, 686
HpyF10VI GCNNNNNNNGC 1 cut(s) 457
HpyF3I CTNAG 1 cut(s) 299
HpySE526I ACGT 4 cut(s) 11, 56, 255, 545
Hsp92I GRCGYC 6 cut(s) 255, 347, 378, 657, 714, 738
Hsp92II CATG 3 cut(s) 68, 233, 680
HspAI GCGC 3 cut(s) 347, 657, 738
KasI GGCGCC 3 cut(s) 346, 656, 737
Ksp22I TGATCA 1 cut(s) 145
Kzo9I GATC 5 cut(s) 145, 367, 406, 609, 628
LmnI GCTCC 4 cut(s) 118, 499, 642, 708
LweI GCATC 2 cut(s) 130, 168
MaeI CTAG 1 cut(s) 671
MaeII ACGT 4 cut(s) 11, 56, 255, 545
MaeIII GTNAC 2 cut(s) 80, 194
MalI GATC 5 cut(s) 147, 369, 408, 611, 630
MboI GATC 5 cut(s) 145, 367, 406, 609, 628
MboII GAAGA 2 cut(s) 430, 758
MhlI GDGCHC 3 cut(s) 503, 647, 726
MluCI AATT 1 cut(s) 188
Mly113I GGCGCC 3 cut(s) 347, 657, 738
MlyI GAGTC 3 cut(s) 88, 272, 514
MnlI CCTC 3 cut(s) 179, 308, 471
MroXI GAANNNNTTC 1 cut(s) 46
MseI TTAA 2 cut(s) 200, 414
MslI CAYNNNNRTG 5 cut(s) 141, 228, 414, 636, 681
MspCI CTTAAG 1 cut(s) 199
MspI CCGG 5 cut(s) 38, 309, 497, 524, 660
MspR9I CCNGG 4 cut(s) 38, 39, 498, 727
MvaI CCWGG 1 cut(s) 727
MwoI GCNNNNNNNGC 1 cut(s) 457
NarI GGCGCC 3 cut(s) 347, 657, 738
NciI CCSGG 3 cut(s) 38, 39, 498
NdeII GATC 5 cut(s) 145, 367, 406, 609, 628
NlaIII CATG 3 cut(s) 68, 233, 680
NlaIV GGNNCC 4 cut(s) 313, 348, 658, 739
NmuCI GTSAC 1 cut(s) 80
NspV TTCGAA 1 cut(s) 618
OliI CACNNNNGTG 1 cut(s) 141
PcsI WCGNNNNNNNCGW 4 cut(s) 249, 255, 327, 717
PdmI GAANNNNTTC 1 cut(s) 46
PfeI GAWTC 1 cut(s) 667
PflFI GACNNNGTC 1 cut(s) 254
PkrI GCNGC 1 cut(s) 553
PleI GAGTC 3 cut(s) 87, 271, 514
PluTI GGCGCC 3 cut(s) 350, 660, 741
PmaCI CACGTG 1 cut(s) 57
PmlI CACGTG 1 cut(s) 57
PpsI GAGTC 3 cut(s) 87, 271, 514
Ppu21I YACGTR 1 cut(s) 57
PshBI ATTAAT 1 cut(s) 414
Psp124BI GAGCTC 1 cut(s) 647
Psp1406I AACGTT 1 cut(s) 11
Psp6I CCWGG 1 cut(s) 725
PspCI CACGTG 1 cut(s) 57
PspGI CCWGG 1 cut(s) 725
PspN4I GGNNCC 4 cut(s) 313, 348, 658, 739
PspPI GGNCC 1 cut(s) 729
PsyI GACNNNGTC 1 cut(s) 254
RseI CAYNNNNRTG 5 cut(s) 141, 228, 414, 636, 681
SacI GAGCTC 1 cut(s) 647
SaqAI TTAA 2 cut(s) 200, 414
SatI GCNGC 1 cut(s) 552
Sau3AI GATC 5 cut(s) 145, 367, 406, 609, 628
Sau96I GGNCC 1 cut(s) 729
SchI GAGTC 3 cut(s) 88, 272, 514
ScrFI CCNGG 4 cut(s) 38, 39, 498, 727
SduI GDGCHC 3 cut(s) 503, 647, 726
SfaNI GCATC 2 cut(s) 130, 168
SfoI GGCGCC 3 cut(s) 348, 658, 739
SfuI TTCGAA 1 cut(s) 618
SgrAI CRCCGGYG 1 cut(s) 659
SinI GGWCC 1 cut(s) 729
SmaI CCCGGG 1 cut(s) 39
SmiMI CAYNNNNRTG 5 cut(s) 141, 228, 414, 636, 681
SmlI CTYRAG 2 cut(s) 125, 199
SmoI CTYRAG 2 cut(s) 125, 199
Sse9I AATT 1 cut(s) 188
SsiI CCGC 2 cut(s) 357, 551
SspDI GGCGCC 3 cut(s) 346, 656, 737
SspI AATATT 1 cut(s) 45
SspMI CTAG 1 cut(s) 671
SstI GAGCTC 1 cut(s) 647
StyD4I CCNGG 4 cut(s) 36, 37, 496, 725
StyI CCWWGG 1 cut(s) 741
TaaI ACNGT 1 cut(s) 331
TaiI ACGT 4 cut(s) 14, 59, 258, 548
TaqI TCGA 4 cut(s) 108, 249, 390, 618
TasI AATT 1 cut(s) 188
TauI GCSGC 1 cut(s) 554
TfiI GAWTC 1 cut(s) 667
Tru1I TTAA 2 cut(s) 200, 414
Tru9I TTAA 2 cut(s) 200, 414
TseFI GTSAC 1 cut(s) 80
Tsp45I GTSAC 1 cut(s) 80
TspDTI ATGAA 1 cut(s) 432
TspGWI ACGGA 3 cut(s) 222, 648, 721
TspMI CCCGGG 1 cut(s) 37
Tth111I GACNNNGTC 1 cut(s) 254
Vha464I CTTAAG 1 cut(s) 199
VpaK11BI GGWCC 1 cut(s) 729
VspI ATTAAT 1 cut(s) 414
XapI RAATTY 1 cut(s) 188
XmaI CCCGGG 1 cut(s) 37
XmnI GAANNNNTTC 1 cut(s) 46
XspI CTAG 1 cut(s) 671
ZraI GACGTC 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.