Rh4BG096300

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000309
Physical Location & Seq
Forward (+)
16425171 .. 16426112
942 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG096300.1

Sequence Viewer

Length: 942 bp
ATGGCCTCAAAAACTCTCCCTACTCCCTTCACAATACTCACTCTCAATCTCCTTTTCATTCTCCTTTCTCTCCTCTCTTGTCCAACTTCATCAGAATCCCAGAGTCACACAAACTCATCATCATCGCCATTCGAGTTTCTGGAGCATCTCAAGGGGTCTCACAAGGGTGATCACATTCAAGGCATCCACGACCTCAAACTCTACCTTGAAAAATTCGGTTACTTAAGCTACGGAAACGACAAGAACCACACTCATGCTGACGATGACGATTTCGACGACGTCGTTGAGTCGGCCGTCAAGGACTACCAGCACTACTACCACCTCAGAACCACCGGAACCCTTGACGGCAAAACCGTTTCCAAGATGATGACGCCCCGTTGCGGTGTGCCAGATCATATTGGCTTCCACAAAATCGACAATAGTAAAAAGGATCACATTAATGAAGTCTCTCACTATTCTTTCTTCAGCAAAAAGCAAAAATGGCCTCCTGCCAAGTACCACCTCACCTACGCTTTCGCTCCGGGCACTCCATCCGAAGCCAAGACTCCGGTTGCACAAGCTTTTCAAACGTGGGCTTCCAGCACTCACTTCAAGTTCAGACGACACAAAGATTACAAGAAGGCTGACATAAAGATCAGTTTCGAACAAGGGGATCACGGAGATGGGAGCTCATTTGACGGCGCCGGTGGGATTCTAGCTCATGCTTATGCACCGACTTATGGAAAGCTCCACTACGACGCCGACGAGCCCTGGTCCGTCGGCGCCTTGGAAGGTTATTATGACTTGGAGACAGTGGCTTTGCATGAGATTGGTCACCTTCTCGGACTTGCTCATAGCTCGGTTGAGGGAGCTATCATGTACCCTTACATCTCTTCTGGAGTTACCAAGCTGGATTTGCATCATGACGATATTCACGGAATTAGAGCTTTATACAAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

313

Amino Acids

35.02

Weight (kDa)

6.32

Isoelectric Point (pI)

42.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 62 - 122 9.6e-10 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 161 - 311 2.4e-48 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 282
AccB1I GGYRCC 2 cut(s) 680, 761
AciI CCGC 1 cut(s) 381
AclWI GGATC 2 cut(s) 438, 660
AcoI YGGCCR 1 cut(s) 291
AcsI RAATTY 1 cut(s) 212
AcuI CTGAAG 1 cut(s) 448
AcyI GRCGYC 5 cut(s) 279, 371, 681, 738, 762
AfaI GTAC 2 cut(s) 497, 860
AfiI CCNNNNNNNGG 2 cut(s) 380, 719
AflII CTTAAG 1 cut(s) 223
AgsI TTSAA 4 cut(s) 179, 209, 566, 592
AjnI CCWGG 1 cut(s) 749
AleI CACNNNNGTG 1 cut(s) 165
AluBI AGCT 9 cut(s) 228, 560, 669, 698, 727, 837, 851, 889, 926
AluI AGCT 9 cut(s) 228, 560, 669, 698, 727, 837, 851, 889, 926
Alw21I GWGCWC 1 cut(s) 671
Alw26I GTCTC 3 cut(s) 162, 451, 782
AlwI GGATC 2 cut(s) 438, 660
AoxI GGCC 3 cut(s) 3, 291, 482
ApoI RAATTY 1 cut(s) 212
ArsI GACNNNNNNTTYG 2 cut(s) 781, 813
AseI ATTAAT 1 cut(s) 438
AspLEI GCGC 2 cut(s) 683, 764
AspS9I GGNCC 1 cut(s) 753
AsuC2I CCSGG 1 cut(s) 522
AsuHPI GGTGA 3 cut(s) 179, 496, 806
AsuII TTCGAA 1 cut(s) 642
AvaII GGWCC 1 cut(s) 753
BaeGI GKGCMC 1 cut(s) 527
BanI GGYRCC 2 cut(s) 680, 761
BanII GRGCYC 2 cut(s) 671, 750
Bbv12I GWGCWC 1 cut(s) 671
BccI CCATC 2 cut(s) 538, 656
BceAI ACGGC 3 cut(s) 278, 361, 694
BciT130I CCWGG 1 cut(s) 751
BclI TGATCA 1 cut(s) 169
BcnI CCSGG 1 cut(s) 522
BcoDI GTCTC 3 cut(s) 162, 451, 782
BfaI CTAG 1 cut(s) 695
BfoI RGCGCY 2 cut(s) 684, 765
BfrI CTTAAG 1 cut(s) 223
Bme1390I CCNGG 2 cut(s) 522, 751
Bme18I GGWCC 1 cut(s) 753
BmgT120I GGNCC 1 cut(s) 753
BmiI GGNNCC 3 cut(s) 337, 682, 763
BmrFI CCNGG 2 cut(s) 522, 751
BmsI GCATC 3 cut(s) 154, 192, 907
BpmI CTGGAG 2 cut(s) 161, 897
Bpu14I TTCGAA 1 cut(s) 642
BpuEI CTTGAG 1 cut(s) 134
BpuMI CCSGG 1 cut(s) 522
BsaBI GATNNNNATC 1 cut(s) 897
BsaHI GRCGYC 5 cut(s) 279, 371, 681, 738, 762
BsaI GGTCTC 1 cut(s) 162
BsaJI CCNNGG 2 cut(s) 749, 765
BsaWI WCCGGW 2 cut(s) 332, 547
Bsc4I CCNNNNNNNGG 2 cut(s) 380, 719
Bse118I RCCGGY 1 cut(s) 683
Bse8I GATNNNNATC 1 cut(s) 897
BseBI CCWGG 1 cut(s) 751
BseDI CCNNGG 2 cut(s) 749, 765
BseGI GGATG 2 cut(s) 183, 530
BseJI GATNNNNATC 1 cut(s) 897
BseLI CCNNNNNNNGG 2 cut(s) 380, 719
BseMII CTCAG 1 cut(s) 337
BseRI GAGGAG 1 cut(s) 62
BseSI GKGCMC 1 cut(s) 527
BseX3I CGGCCG 1 cut(s) 291
Bsh1285I CGRYCG 1 cut(s) 294
BshFI GGCC 3 cut(s) 5, 293, 484
BshNI GGYRCC 2 cut(s) 680, 761
BsiEI CGRYCG 1 cut(s) 294
BsiHKAI GWGCWC 1 cut(s) 671
BsiSI CCGG 4 cut(s) 333, 521, 548, 684
BslI CCNNNNNNNGG 2 cut(s) 380, 719
BsmAI GTCTC 3 cut(s) 162, 451, 782
BsnI GGCC 3 cut(s) 5, 293, 484
Bso31I GGTCTC 1 cut(s) 162
Bsp119I TTCGAA 1 cut(s) 642
Bsp1286I GDGCHC 3 cut(s) 527, 671, 750
Bsp143I GATC 5 cut(s) 169, 391, 430, 633, 652
BspACI CCGC 1 cut(s) 381
BspANI GGCC 3 cut(s) 5, 293, 484
BspCNI CTCAG 1 cut(s) 336
BspHI TCATGA 1 cut(s) 901
BspLI GGNNCC 3 cut(s) 337, 682, 763
BspPI GGATC 2 cut(s) 438, 660
BspT104I TTCGAA 1 cut(s) 642
BspT107I GGYRCC 2 cut(s) 680, 761
BspTI CTTAAG 1 cut(s) 223
BspTNI GGTCTC 1 cut(s) 162
BsrFI RCCGGY 1 cut(s) 683
BssAI RCCGGY 1 cut(s) 683
BssECI CCNNGG 2 cut(s) 749, 765
BssMI GATC 5 cut(s) 169, 391, 430, 633, 652
BssNI GRCGYC 5 cut(s) 279, 371, 681, 738, 762
BssT1I CCWWGG 1 cut(s) 765
Bst2UI CCWGG 1 cut(s) 751
Bst4CI ACNGT 2 cut(s) 355, 793
Bst6I CTCTTC 1 cut(s) 877
BstACI GRCGYC 5 cut(s) 279, 371, 681, 738, 762
BstAFI CTTAAG 1 cut(s) 223
BstBI TTCGAA 1 cut(s) 642
BstDEI CTNAG 1 cut(s) 323
BstEII GGTNACC 1 cut(s) 812
BstF5I GGATG 2 cut(s) 183, 530
BstH2I RGCGCY 2 cut(s) 684, 765
BstHHI GCGC 2 cut(s) 683, 764
BstKTI GATC 5 cut(s) 172, 394, 433, 636, 655
BstMAI GTCTC 3 cut(s) 162, 451, 782
BstMBI GATC 5 cut(s) 169, 391, 430, 633, 652
BstMCI CGRYCG 1 cut(s) 294
BstMWI GCNNNNNNNGC 2 cut(s) 481, 895
BstNI CCWGG 1 cut(s) 751
BstPI GGTNACC 1 cut(s) 812
BstSCI CCNGG 2 cut(s) 520, 749
BstSLI GKGCMC 1 cut(s) 527
BstZI CGGCCG 1 cut(s) 291
BsuRI GGCC 3 cut(s) 5, 293, 484
BtgZI GCGATG 1 cut(s) 108
BtsCI GGATG 2 cut(s) 183, 530
BtsIMutI CAGTG 1 cut(s) 798
CciI TCATGA 1 cut(s) 901
CfoI GCGC 2 cut(s) 683, 764
Cfr10I RCCGGY 1 cut(s) 683
Cfr13I GGNCC 1 cut(s) 753
CseI GACGC 2 cut(s) 379, 746
Csp6I GTAC 2 cut(s) 496, 859
CviAII CATG 5 cut(s) 254, 701, 803, 856, 902
CviQI GTAC 2 cut(s) 496, 859
DdeI CTNAG 1 cut(s) 323
DinI GGCGCC 2 cut(s) 682, 763
DpnI GATC 5 cut(s) 171, 393, 432, 635, 654
DpnII GATC 5 cut(s) 169, 391, 430, 633, 652
EaeI YGGCCR 1 cut(s) 291
EagI CGGCCG 1 cut(s) 291
Eam1104I CTCTTC 1 cut(s) 877
EarI CTCTTC 1 cut(s) 877
Ecl136II GAGCTC 1 cut(s) 669
EclXI CGGCCG 1 cut(s) 291
Eco130I CCWWGG 1 cut(s) 765
Eco24I GRGCYC 2 cut(s) 671, 750
Eco31I GGTCTC 1 cut(s) 162
Eco47I GGWCC 1 cut(s) 753
Eco52I CGGCCG 1 cut(s) 291
Eco53kI GAGCTC 1 cut(s) 669
Eco57I CTGAAG 1 cut(s) 448
Eco91I GGTNACC 1 cut(s) 812
EcoICRI GAGCTC 1 cut(s) 669
EcoO65I GGTNACC 1 cut(s) 812
EcoRII CCWGG 1 cut(s) 749
EcoT14I CCWWGG 1 cut(s) 765
EcoT38I GRGCYC 2 cut(s) 671, 750
EgeI GGCGCC 2 cut(s) 682, 763
EheI GGCGCC 2 cut(s) 682, 763
ErhI CCWWGG 1 cut(s) 765
FaeI CATG 5 cut(s) 257, 704, 806, 859, 905
FatI CATG 5 cut(s) 253, 700, 802, 855, 901
FbaI TGATCA 1 cut(s) 169
FokI GGATG 2 cut(s) 170, 517
FriOI GRGCYC 2 cut(s) 671, 750
FspBI CTAG 1 cut(s) 695
GlaI GCGC 2 cut(s) 682, 763
GsuI CTGGAG 2 cut(s) 161, 897
HaeII RGCGCY 2 cut(s) 684, 765
HaeIII GGCC 3 cut(s) 5, 293, 484
HapII CCGG 4 cut(s) 333, 521, 548, 684
HgaI GACGC 2 cut(s) 379, 746
HhaI GCGC 2 cut(s) 683, 764
Hin1I GRCGYC 5 cut(s) 279, 371, 681, 738, 762
Hin1II CATG 5 cut(s) 257, 704, 806, 859, 905
Hin6I GCGC 2 cut(s) 681, 762
HinP1I GCGC 2 cut(s) 681, 762
HindIII AAGCTT 1 cut(s) 558
HinfI GANTC 5 cut(s) 95, 103, 287, 544, 691
HpaII CCGG 4 cut(s) 333, 521, 548, 684
HphI GGTGA 3 cut(s) 179, 496, 806
Hpy188I TCNGA 5 cut(s) 94, 326, 535, 599, 824
Hpy188III TCNNGA 4 cut(s) 140, 876, 902, 939
Hpy99I CGWCG 6 cut(s) 278, 281, 284, 740, 746, 761
HpyAV CCTTC 4 cut(s) 37, 613, 764, 827
HpyCH4III ACNGT 2 cut(s) 355, 793
HpyCH4IV ACGT 2 cut(s) 279, 569
HpyCH4V TGCA 4 cut(s) 554, 710, 802, 898
HpyF10VI GCNNNNNNNGC 2 cut(s) 481, 895
HpyF3I CTNAG 1 cut(s) 323
HpySE526I ACGT 2 cut(s) 279, 569
Hsp92I GRCGYC 5 cut(s) 279, 371, 681, 738, 762
Hsp92II CATG 5 cut(s) 257, 704, 806, 859, 905
HspAI GCGC 2 cut(s) 681, 762
KasI GGCGCC 2 cut(s) 680, 761
Ksp22I TGATCA 1 cut(s) 169
Kzo9I GATC 5 cut(s) 169, 391, 430, 633, 652
LmnI GCTCC 5 cut(s) 142, 523, 666, 732, 848
LweI GCATC 3 cut(s) 154, 192, 907
MaeI CTAG 1 cut(s) 695
MaeII ACGT 2 cut(s) 279, 569
MaeIII GTNAC 4 cut(s) 104, 218, 812, 880
MalI GATC 5 cut(s) 171, 393, 432, 635, 654
MboI GATC 5 cut(s) 169, 391, 430, 633, 652
MboII GAAGA 2 cut(s) 454, 864
MhlI GDGCHC 3 cut(s) 527, 671, 750
MluCI AATT 2 cut(s) 212, 918
Mly113I GGCGCC 2 cut(s) 681, 762
MlyI GAGTC 3 cut(s) 112, 296, 538
MmeI TCCRAC 1 cut(s) 107
MnlI CCTC 7 cut(s) 16, 83, 203, 332, 495, 512, 838
MseI TTAA 2 cut(s) 224, 438
MslI CAYNNNNRTG 5 cut(s) 165, 252, 438, 660, 705
MspCI CTTAAG 1 cut(s) 223
MspI CCGG 4 cut(s) 333, 521, 548, 684
MspR9I CCNGG 2 cut(s) 522, 751
MvaI CCWGG 1 cut(s) 751
MwoI GCNNNNNNNGC 2 cut(s) 481, 895
NarI GGCGCC 2 cut(s) 681, 762
NciI CCSGG 1 cut(s) 522
NdeII GATC 5 cut(s) 169, 391, 430, 633, 652
NlaIII CATG 5 cut(s) 257, 704, 806, 859, 905
NlaIV GGNNCC 3 cut(s) 337, 682, 763
NmuCI GTSAC 2 cut(s) 104, 812
NspV TTCGAA 1 cut(s) 642
OliI CACNNNNGTG 1 cut(s) 165
PagI TCATGA 1 cut(s) 901
PcsI WCGNNNNNNNCGW 4 cut(s) 273, 279, 351, 741
PfeI GAWTC 2 cut(s) 95, 691
PflFI GACNNNGTC 1 cut(s) 278
PleI GAGTC 3 cut(s) 111, 295, 538
PluTI GGCGCC 2 cut(s) 684, 765
PpsI GAGTC 3 cut(s) 111, 295, 538
PshBI ATTAAT 1 cut(s) 438
Psp124BI GAGCTC 1 cut(s) 671
Psp6I CCWGG 1 cut(s) 749
PspEI GGTNACC 1 cut(s) 812
PspGI CCWGG 1 cut(s) 749
PspN4I GGNNCC 3 cut(s) 337, 682, 763
PspPI GGNCC 1 cut(s) 753
PsyI GACNNNGTC 1 cut(s) 278
RsaI GTAC 2 cut(s) 497, 860
RsaNI GTAC 2 cut(s) 496, 859
RseI CAYNNNNRTG 5 cut(s) 165, 252, 438, 660, 705
SacI GAGCTC 1 cut(s) 671
SaqAI TTAA 2 cut(s) 224, 438
Sau3AI GATC 5 cut(s) 169, 391, 430, 633, 652
Sau96I GGNCC 1 cut(s) 753
SchI GAGTC 3 cut(s) 112, 296, 538
ScrFI CCNGG 2 cut(s) 522, 751
SduI GDGCHC 3 cut(s) 527, 671, 750
SfaNI GCATC 3 cut(s) 154, 192, 907
SfoI GGCGCC 2 cut(s) 682, 763
SfuI TTCGAA 1 cut(s) 642
SgrAI CRCCGGYG 1 cut(s) 683
SinI GGWCC 1 cut(s) 753
SmiMI CAYNNNNRTG 5 cut(s) 165, 252, 438, 660, 705
SmlI CTYRAG 2 cut(s) 149, 223
SmoI CTYRAG 2 cut(s) 149, 223
Sse9I AATT 2 cut(s) 212, 918
SsiI CCGC 1 cut(s) 381
SspDI GGCGCC 2 cut(s) 680, 761
SspMI CTAG 1 cut(s) 695
SstI GAGCTC 1 cut(s) 671
StyD4I CCNGG 2 cut(s) 520, 749
StyI CCWWGG 1 cut(s) 765
TaaI ACNGT 2 cut(s) 355, 793
TaiI ACGT 2 cut(s) 282, 572
TaqI TCGA 4 cut(s) 132, 273, 414, 642
TasI AATT 2 cut(s) 212, 918
TfiI GAWTC 2 cut(s) 95, 691
Tru1I TTAA 2 cut(s) 224, 438
Tru9I TTAA 2 cut(s) 224, 438
TscAI CASTG 1 cut(s) 798
TseFI GTSAC 2 cut(s) 104, 812
Tsp45I GTSAC 2 cut(s) 104, 812
TspDTI ATGAA 3 cut(s) 46, 78, 456
TspGWI ACGGA 4 cut(s) 246, 672, 745, 930
TspRI CASTG 1 cut(s) 798
Tth111I GACNNNGTC 1 cut(s) 278
Vha464I CTTAAG 1 cut(s) 223
VpaK11BI GGWCC 1 cut(s) 753
VspI ATTAAT 1 cut(s) 438
XapI RAATTY 1 cut(s) 212
XspI CTAG 1 cut(s) 695
ZraI GACGTC 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.