Rw2G051270

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000309
Physical Location & Seq
Reverse (-)
79023822 .. 79026361
2540 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G051270.1

Sequence Viewer

Length: 672 bp
ATGCCAAACTTCAATATTTTGGGTTCTTTTGATTGGTACAGAGAGAAACACAAGCAGAGCTATTTCTTACCTGCCCAGAATTCCTCTGCCACTGCTGCCGCCGCCGCTCTGCTCCGTCAAATCTCTTCTTTGGTTCTTAGGTCCTTCCTCTTTCTTCGAGGTGCAATTCATACCACCGTTCATTACTCATTTCCCTATGGAAATCTAAAATGGCCTTCTTCAAAATACCATCTGGCTTACCGTTTTCTCGCCGGCACCCCATCCGAAGCCCCGGGTGCTGTCGCACAGGCTTTCTCAACATGGGCTAAGAACACTCACTTCAAATTCAGCAAGGCTCAAAATCACCAGAGTGCGGATTTGAAGGTTAGCTTTCATAAGGGTGATCATAGAGACGGGAATTCATTTGATGGGCCTAGCGGACAGCTGGCCCATGCTGCTTACCCTACGGAGGGAACATTGCACTACGAAGCAGATGAGACTTGGTCTGTAGGTGCTAAACCAGGTGCCATAGACATTGAGAGTGTTGGTTTGCATGAAATAGGGCACCTTCTGGGACTTGGACACAGCTCAGTGGAGGAAGCTATCATGTATCCAACCATCCCCGAGGGAGTGGCTCATAAAAGTTTGCATGAGGACGATATTCAAGGAATAAAAGCTTTATACAACGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

223

Amino Acids

24.51

Weight (kDa)

6.73

Isoelectric Point (pI)

39.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M10 PF00413 71 - 221 7.1e-45 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 79
AccB1I GGYRCC 3 cut(s) 254, 503, 543
AccBSI CCGCTC 1 cut(s) 107
AciI CCGC 5 cut(s) 99, 102, 105, 353, 417
AclI AACGTT 1 cut(s) 666
AcsI RAATTY 3 cut(s) 79, 323, 397
AfaI GTAC 1 cut(s) 38
AfiI CCNNNNNNNGG 3 cut(s) 352, 448, 449
AgsI TTSAA 5 cut(s) 13, 222, 322, 361, 644
AjnI CCWGG 1 cut(s) 499
AleI CACNNNNGTG 1 cut(s) 348
AluBI AGCT 6 cut(s) 60, 369, 424, 567, 581, 656
AluI AGCT 6 cut(s) 60, 369, 424, 567, 581, 656
Alw26I GTCTC 2 cut(s) 384, 470
Ama87I CYCGRG 2 cut(s) 271, 602
AoxI GGCC 3 cut(s) 212, 410, 426
ApeKI GCWGC 2 cut(s) 95, 434
ApoI RAATTY 3 cut(s) 79, 323, 397
AspS9I GGNCC 3 cut(s) 141, 410, 427
AsuC2I CCSGG 2 cut(s) 272, 273
AsuHPI GGTGA 2 cut(s) 335, 392
AvaI CYCGRG 2 cut(s) 271, 602
AvaII GGWCC 1 cut(s) 141
BaeGI GKGCMC 1 cut(s) 546
BanI GGYRCC 3 cut(s) 254, 503, 543
BbvI GCAGC 2 cut(s) 82, 421
BccI CCATC 4 cut(s) 237, 268, 401, 605
BciT130I CCWGG 1 cut(s) 501
BciVI GTATCC 1 cut(s) 600
BclI TGATCA 1 cut(s) 382
BcnI CCSGG 2 cut(s) 272, 273
BcoDI GTCTC 2 cut(s) 384, 470
BfaI CTAG 1 cut(s) 414
BfmI CTRYAG 1 cut(s) 486
BfuAI ACCTGC 1 cut(s) 79
BfuI GTATCC 1 cut(s) 600
BisI GCNGC 5 cut(s) 96, 99, 102, 105, 435
BlsI GCNGC 5 cut(s) 97, 100, 103, 106, 436
Bme1390I CCNGG 3 cut(s) 272, 273, 501
Bme18I GGWCC 1 cut(s) 141
BmeT110I CYCGRG 2 cut(s) 271, 602
BmgT120I GGNCC 3 cut(s) 141, 410, 427
BmiI GGNNCC 3 cut(s) 256, 505, 545
BmrFI CCNGG 3 cut(s) 272, 273, 501
BpuMI CCSGG 2 cut(s) 272, 273
BsaJI CCNNGG 3 cut(s) 270, 271, 603
Bsc4I CCNNNNNNNGG 3 cut(s) 352, 448, 449
Bse118I RCCGGY 1 cut(s) 251
Bse3DI GCAATG 1 cut(s) 455
BseBI CCWGG 1 cut(s) 501
BseDI CCNNGG 3 cut(s) 270, 271, 603
BseGI GGATG 2 cut(s) 260, 597
BseLI CCNNNNNNNGG 3 cut(s) 352, 448, 449
BseMI GCAATG 1 cut(s) 455
BseMII CTCAG 1 cut(s) 582
BseSI GKGCMC 1 cut(s) 546
BseXI GCAGC 2 cut(s) 82, 421
BshFI GGCC 3 cut(s) 214, 412, 428
BshNI GGYRCC 3 cut(s) 254, 503, 543
BsiHKCI CYCGRG 2 cut(s) 271, 602
BsiSI CCGG 2 cut(s) 252, 272
BslFI GGGAC 1 cut(s) 567
BslI CCNNNNNNNGG 3 cut(s) 352, 448, 449
BsmAI GTCTC 2 cut(s) 384, 470
BsmBI CGTCTC 1 cut(s) 384
BsmFI GGGAC 1 cut(s) 567
BsnI GGCC 3 cut(s) 214, 412, 428
BsoBI CYCGRG 2 cut(s) 271, 602
Bsp1286I GDGCHC 1 cut(s) 546
Bsp143I GATC 1 cut(s) 382
BspACI CCGC 5 cut(s) 99, 102, 105, 353, 417
BspANI GGCC 3 cut(s) 214, 412, 428
BspCNI CTCAG 1 cut(s) 581
BspLI GGNNCC 3 cut(s) 256, 505, 545
BspMI ACCTGC 1 cut(s) 79
BspT107I GGYRCC 3 cut(s) 254, 503, 543
BsrBI CCGCTC 1 cut(s) 107
BsrDI GCAATG 1 cut(s) 455
BsrFI RCCGGY 1 cut(s) 251
BssAI RCCGGY 1 cut(s) 251
BssECI CCNNGG 3 cut(s) 270, 271, 603
BssMI GATC 1 cut(s) 382
Bst2UI CCWGG 1 cut(s) 501
Bst4CI ACNGT 2 cut(s) 178, 242
Bst6I CTCTTC 1 cut(s) 130
BstC8I GCNNGC 2 cut(s) 253, 426
BstDEI CTNAG 3 cut(s) 137, 306, 568
BstF5I GGATG 2 cut(s) 260, 597
BstKTI GATC 1 cut(s) 385
BstMAI GTCTC 2 cut(s) 384, 470
BstMBI GATC 1 cut(s) 382
BstMWI GCNNNNNNNGC 5 cut(s) 95, 101, 104, 275, 434
BstNI CCWGG 1 cut(s) 501
BstSCI CCNGG 3 cut(s) 270, 271, 499
BstSFI CTRYAG 1 cut(s) 486
BstSLI GKGCMC 1 cut(s) 546
BstV1I GCAGC 2 cut(s) 82, 421
BsuI GTATCC 1 cut(s) 600
BsuRI GGCC 3 cut(s) 214, 412, 428
BtsCI GGATG 2 cut(s) 260, 597
BtsI GCAGTG 1 cut(s) 90
BtsIMutI CAGTG 2 cut(s) 90, 576
BveI ACCTGC 1 cut(s) 79
Cac8I GCNNGC 2 cut(s) 253, 426
Cfr10I RCCGGY 1 cut(s) 251
Cfr13I GGNCC 3 cut(s) 141, 410, 427
Cfr9I CCCGGG 1 cut(s) 271
CsiI ACCWGGT 1 cut(s) 499
Csp6I GTAC 1 cut(s) 37
CviAII CATG 5 cut(s) 300, 431, 533, 586, 629
CviQI GTAC 1 cut(s) 37
DdeI CTNAG 3 cut(s) 137, 306, 568
DpnI GATC 1 cut(s) 384
DpnII GATC 1 cut(s) 382
Eam1104I CTCTTC 1 cut(s) 130
EarI CTCTTC 1 cut(s) 130
Eco47I GGWCC 1 cut(s) 141
Eco88I CYCGRG 2 cut(s) 271, 602
EcoO109I RGGNCCY 1 cut(s) 141
EcoRI GAATTC 2 cut(s) 79, 397
EcoRII CCWGG 1 cut(s) 499
Esp3I CGTCTC 1 cut(s) 384
FaeI CATG 5 cut(s) 303, 434, 536, 589, 632
FalI AAGNNNNNCTT 2 cut(s) 353, 385
FaqI GGGAC 1 cut(s) 567
FatI CATG 5 cut(s) 299, 430, 532, 585, 628
FbaI TGATCA 1 cut(s) 382
Fnu4HI GCNGC 5 cut(s) 96, 99, 102, 105, 435
FokI GGATG 2 cut(s) 247, 584
Fsp4HI GCNGC 5 cut(s) 96, 99, 102, 105, 435
FspBI CTAG 1 cut(s) 414
GluI GCNGC 5 cut(s) 96, 99, 102, 105, 435
HaeIII GGCC 3 cut(s) 214, 412, 428
HapII CCGG 2 cut(s) 252, 272
Hin1II CATG 5 cut(s) 303, 434, 536, 589, 632
HindIII AAGCTT 1 cut(s) 654
HpaII CCGG 2 cut(s) 252, 272
HphI GGTGA 2 cut(s) 335, 392
Hpy188I TCNGA 1 cut(s) 265
HpyAV CCTTC 4 cut(s) 154, 225, 355, 557
HpyCH4III ACNGT 2 cut(s) 178, 242
HpyCH4IV ACGT 1 cut(s) 666
HpyCH4V TGCA 4 cut(s) 164, 460, 532, 628
HpyF10VI GCNNNNNNNGC 5 cut(s) 95, 101, 104, 275, 434
HpyF3I CTNAG 3 cut(s) 137, 306, 568
HpySE526I ACGT 1 cut(s) 666
Hsp92II CATG 5 cut(s) 303, 434, 536, 589, 632
KroI GCCGGC 1 cut(s) 251
KroNI GCCGGC 1 cut(s) 253
Ksp22I TGATCA 1 cut(s) 382
Kzo9I GATC 1 cut(s) 382
LmnI GCTCC 1 cut(s) 117
Lsp1109I GCAGC 2 cut(s) 82, 421
MabI ACCWGGT 1 cut(s) 499
MaeI CTAG 1 cut(s) 414
MaeII ACGT 1 cut(s) 666
MalI GATC 1 cut(s) 384
MbiI CCGCTC 1 cut(s) 107
MboI GATC 1 cut(s) 382
MboII GAAGA 3 cut(s) 117, 146, 210
MhlI GDGCHC 1 cut(s) 546
MluCI AATT 4 cut(s) 79, 165, 323, 397
MmeI TCCRAC 1 cut(s) 617
MnlI CCTC 7 cut(s) 94, 152, 158, 442, 568, 598, 625
MroNI GCCGGC 1 cut(s) 251
MslI CAYNNNNRTG 2 cut(s) 348, 378
MspA1I CMGCKG 1 cut(s) 424
MspI CCGG 2 cut(s) 252, 272
MspR9I CCNGG 3 cut(s) 272, 273, 501
MvaI CCWGG 1 cut(s) 501
MwoI GCNNNNNNNGC 5 cut(s) 95, 101, 104, 275, 434
NaeI GCCGGC 1 cut(s) 253
NciI CCSGG 2 cut(s) 272, 273
NdeII GATC 1 cut(s) 382
NgoMIV GCCGGC 1 cut(s) 251
NlaIII CATG 5 cut(s) 303, 434, 536, 589, 632
NlaIV GGNNCC 3 cut(s) 256, 505, 545
OliI CACNNNNGTG 1 cut(s) 348
PdiI GCCGGC 1 cut(s) 253
PflFI GACNNNGTC 1 cut(s) 481
PkrI GCNGC 5 cut(s) 97, 100, 103, 106, 436
PpuMI RGGWCCY 1 cut(s) 141
Psp1406I AACGTT 1 cut(s) 666
Psp5II RGGWCCY 1 cut(s) 141
Psp6I CCWGG 1 cut(s) 499
PspGI CCWGG 1 cut(s) 499
PspN4I GGNNCC 3 cut(s) 256, 505, 545
PspPI GGNCC 3 cut(s) 141, 410, 427
PspPPI RGGWCCY 1 cut(s) 141
PsyI GACNNNGTC 1 cut(s) 481
PvuII CAGCTG 1 cut(s) 424
RsaI GTAC 1 cut(s) 38
RsaNI GTAC 1 cut(s) 37
RseI CAYNNNNRTG 2 cut(s) 348, 378
SatI GCNGC 5 cut(s) 96, 99, 102, 105, 435
Sau3AI GATC 1 cut(s) 382
Sau96I GGNCC 3 cut(s) 141, 410, 427
ScrFI CCNGG 3 cut(s) 272, 273, 501
SduI GDGCHC 1 cut(s) 546
SexAI ACCWGGT 1 cut(s) 499
SfcI CTRYAG 1 cut(s) 486
SinI GGWCC 1 cut(s) 141
SmaI CCCGGG 1 cut(s) 273
SmiMI CAYNNNNRTG 2 cut(s) 348, 378
Sse9I AATT 4 cut(s) 79, 165, 323, 397
SsiI CCGC 5 cut(s) 99, 102, 105, 353, 417
SspI AATATT 1 cut(s) 16
SspMI CTAG 1 cut(s) 414
StyD4I CCNGG 3 cut(s) 270, 271, 499
TaaI ACNGT 2 cut(s) 178, 242
TaiI ACGT 1 cut(s) 669
TaqI TCGA 1 cut(s) 157
TasI AATT 4 cut(s) 79, 165, 323, 397
TauI GCSGC 3 cut(s) 101, 104, 107
TscAI CASTG 2 cut(s) 97, 576
TseI GCWGC 2 cut(s) 95, 434
TspDTI ATGAA 5 cut(s) 158, 170, 362, 390, 549
TspGWI ACGGA 2 cut(s) 104, 461
TspMI CCCGGG 1 cut(s) 271
TspRI CASTG 2 cut(s) 97, 576
Tth111I GACNNNGTC 1 cut(s) 481
VpaK11BI GGWCC 1 cut(s) 141
XapI RAATTY 3 cut(s) 79, 323, 397
XmaI CCCGGG 1 cut(s) 271
XspI CTAG 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.