FvH4_6g50010

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000309
Physical Location & Seq
Reverse (-)
37310833 .. 37312263
1431 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g50010.t1

Sequence Viewer

Length: 927 bp
ATGGCATCCAAGTCTAATCTGTCACTCTTCACTATAACTCTCCTCCTCGTCCTTCTTACGCTCCTCTGTTATGCAACTGCTTCTCATCCCCACAACAAGAGCTCTTCCCCAGTTGAGTTCCTTGAACATCTCAAGGGTTGCCACAAAGGTGATAAGGTCAAGGGCATCAATGACCTCAAAAAGTACCTTGAAAAATACGGGTACTTGAACTACAAGAACCACAGTCATTCAGACGACGATGAATTCGACGATCTTTTGGAGGAAGCTGTCAAGACCTACCAGATCAATTACCACCTCAAGTCCACCGGAACATTGGACAGCAAAACAGTGTCGAAGATGACAACGCCTCGTTGTGGCGTGGCAGATATCATCAATGGCACCTCGTCCATGCGATCAGCCAAGAAAAGGCACCACCACGGTTCACTTCATACTACTGCTCACTACTCGTTTTTCCAAGGAAATCCAAAATGGCCTTCCTCTAAGTATCATCTCACTTACGGTTTTCTTCCCAACACTCCATCTGAAGCCACAGGCGCGGTGGCGCGGGCTTTCGCAACATGGGAGGGGAACACTCACTTTACATTCAGCCAGGCACAAAGTTACGAGAATGCGGATTTGAAGATCGGTTTTCAAAGGGGTAATCATGGAGACGGGGCTGCATTTGATGGACCTGGCGGAACGCTGGCCCATGCTTTTAGCCCGACGCACGGAATATTCCACTACGACGCAGATGAGACTTGGTCTGTGGGTGCTGTACCAGGTGCTACTGACTTGGAGACGGTGGCTTTGCATGAGATAGGGCACCTTCTCGGACTGGGACATAGCTCAGTGAAGGGAGCTATCATGGAACCGACTCTTCCCACGGGAGAGACTCGAGGTTTGCATGGGGACGATGTTCAAGGTATCAAGGCTTTATATAACGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

309

Amino Acids

33.61

Weight (kDa)

6.61

Isoelectric Point (pI)

23.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 56 - 113 1.5e-10 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 156 - 306 6.4e-46 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 377, 408, 801
AccII CGCG 2 cut(s) 536, 544
AciI CCGC 4 cut(s) 536, 544, 611, 675
AcsI RAATTY 1 cut(s) 242
AcuI CTGAAG 1 cut(s) 543
AfaI GTAC 3 cut(s) 185, 203, 756
AfiI CCNNNNNNNGG 3 cut(s) 353, 405, 707
AgsI TTSAA 6 cut(s) 125, 191, 208, 619, 632, 899
AjnI CCWGG 3 cut(s) 588, 670, 757
AjuI GAANNNNNNNTTGG 2 cut(s) 457, 489
AleI CACNNNNGTG 1 cut(s) 147
AluBI AGCT 4 cut(s) 102, 266, 825, 839
AluI AGCT 4 cut(s) 102, 266, 825, 839
Alw21I GWGCWC 1 cut(s) 104
Alw26I GTCTC 4 cut(s) 642, 728, 770, 863
Ama87I CYCGRG 1 cut(s) 873
AoxI GGCC 2 cut(s) 470, 684
ApeKI GCWGC 1 cut(s) 656
ApoI RAATTY 1 cut(s) 242
ArsI GACNNNNNNTTYG 6 cut(s) 227, 259, 769, 801, 862, 894
AspLEI GCGC 2 cut(s) 536, 544
AspS9I GGNCC 2 cut(s) 668, 685
AsuHPI GGTGA 1 cut(s) 161
AvaI CYCGRG 1 cut(s) 873
AvaII GGWCC 1 cut(s) 668
BaeGI GKGCMC 1 cut(s) 804
BanI GGYRCC 3 cut(s) 377, 408, 801
BanII GRGCYC 1 cut(s) 104
Bbv12I GWGCWC 1 cut(s) 104
BbvI GCAGC 1 cut(s) 643
BccI CCATC 2 cut(s) 526, 659
BciT130I CCWGG 3 cut(s) 590, 672, 759
BcoDI GTCTC 4 cut(s) 642, 728, 770, 863
BisI GCNGC 1 cut(s) 657
BlsI GCNGC 1 cut(s) 658
Bme1390I CCNGG 3 cut(s) 590, 672, 759
Bme18I GGWCC 1 cut(s) 668
BmeT110I CYCGRG 1 cut(s) 873
BmgT120I GGNCC 2 cut(s) 668, 685
BmiI GGNNCC 4 cut(s) 379, 410, 803, 849
BmrFI CCNGG 3 cut(s) 590, 672, 759
BmrI ACTGGG 2 cut(s) 104, 824
BmsI GCATC 2 cut(s) 14, 174
BmuI ACTGGG 2 cut(s) 104, 824
BpuEI CTTGAG 2 cut(s) 116, 281
BsaJI CCNNGG 3 cut(s) 415, 454, 861
BsaWI WCCGGW 1 cut(s) 305
Bsc4I CCNNNNNNNGG 3 cut(s) 353, 405, 707
Bse1I ACTGG 2 cut(s) 110, 819
BseBI CCWGG 3 cut(s) 590, 672, 759
BseDI CCNNGG 3 cut(s) 415, 454, 861
BseGI GGATG 2 cut(s) 5, 85
BseLI CCNNNNNNNGG 3 cut(s) 353, 405, 707
BseMII CTCAG 1 cut(s) 840
BseNI ACTGG 2 cut(s) 110, 819
BseRI GAGGAG 3 cut(s) 32, 35, 53
BseSI GKGCMC 1 cut(s) 804
BseXI GCAGC 1 cut(s) 643
Bsh1236I CGCG 2 cut(s) 536, 544
BshFI GGCC 2 cut(s) 472, 686
BshNI GGYRCC 3 cut(s) 377, 408, 801
BsiHKAI GWGCWC 1 cut(s) 104
BsiHKCI CYCGRG 1 cut(s) 873
BsiSI CCGG 1 cut(s) 306
BslFI GGGAC 2 cut(s) 831, 902
BslI CCNNNNNNNGG 3 cut(s) 353, 405, 707
BsmAI GTCTC 4 cut(s) 642, 728, 770, 863
BsmBI CGTCTC 2 cut(s) 642, 770
BsmFI GGGAC 2 cut(s) 831, 902
BsmI GAATGC 1 cut(s) 613
BsnI GGCC 2 cut(s) 472, 686
BsoBI CYCGRG 1 cut(s) 873
Bsp1286I GDGCHC 2 cut(s) 104, 804
Bsp143I GATC 4 cut(s) 250, 282, 392, 621
BspACI CCGC 4 cut(s) 536, 544, 611, 675
BspANI GGCC 2 cut(s) 472, 686
BspCNI CTCAG 1 cut(s) 839
BspFNI CGCG 2 cut(s) 536, 544
BspLI GGNNCC 4 cut(s) 379, 410, 803, 849
BspQI GCTCTTC 1 cut(s) 109
BspT107I GGYRCC 3 cut(s) 377, 408, 801
BsrI ACTGG 2 cut(s) 110, 819
BssECI CCNNGG 3 cut(s) 415, 454, 861
BssMI GATC 4 cut(s) 250, 282, 392, 621
BssT1I CCWWGG 1 cut(s) 454
Bst2UI CCWGG 3 cut(s) 590, 672, 759
Bst4CI ACNGT 5 cut(s) 224, 328, 419, 500, 781
Bst6I CTCTTC 3 cut(s) 32, 109, 861
BstC8I GCNNGC 2 cut(s) 546, 684
BstDEI CTNAG 2 cut(s) 480, 826
BstDSI CCRYGG 2 cut(s) 415, 861
BstF5I GGATG 2 cut(s) 5, 85
BstFNI CGCG 2 cut(s) 536, 544
BstHHI GCGC 2 cut(s) 536, 544
BstKTI GATC 4 cut(s) 253, 285, 395, 624
BstMAI GTCTC 4 cut(s) 642, 728, 770, 863
BstMBI GATC 4 cut(s) 250, 282, 392, 621
BstMWI GCNNNNNNNGC 1 cut(s) 533
BstNI CCWGG 3 cut(s) 590, 672, 759
BstSCI CCNGG 3 cut(s) 588, 670, 757
BstSLI GKGCMC 1 cut(s) 804
BstUI CGCG 2 cut(s) 536, 544
BstV1I GCAGC 1 cut(s) 643
BsuRI GGCC 2 cut(s) 472, 686
BtgI CCRYGG 2 cut(s) 415, 861
BtsCI GGATG 2 cut(s) 5, 85
BtsIMutI CAGTG 2 cut(s) 333, 834
Cac8I GCNNGC 2 cut(s) 546, 684
CfoI GCGC 2 cut(s) 536, 544
Cfr13I GGNCC 2 cut(s) 668, 685
CseI GACGC 2 cut(s) 712, 734
CsiI ACCWGGT 1 cut(s) 757
Csp6I GTAC 3 cut(s) 184, 202, 755
CviAII CATG 7 cut(s) 388, 558, 644, 689, 791, 844, 884
CviQI GTAC 3 cut(s) 184, 202, 755
DdeI CTNAG 2 cut(s) 480, 826
DpnI GATC 4 cut(s) 252, 284, 394, 623
DpnII GATC 4 cut(s) 250, 282, 392, 621
Eam1104I CTCTTC 3 cut(s) 32, 109, 861
EarI CTCTTC 3 cut(s) 32, 109, 861
EciI GGCGGA 1 cut(s) 690
Ecl136II GAGCTC 1 cut(s) 102
Eco130I CCWWGG 1 cut(s) 454
Eco24I GRGCYC 1 cut(s) 104
Eco32I GATATC 1 cut(s) 367
Eco47I GGWCC 1 cut(s) 668
Eco53kI GAGCTC 1 cut(s) 102
Eco57I CTGAAG 1 cut(s) 543
Eco88I CYCGRG 1 cut(s) 873
EcoICRI GAGCTC 1 cut(s) 102
EcoRI GAATTC 1 cut(s) 242
EcoRII CCWGG 3 cut(s) 588, 670, 757
EcoRV GATATC 1 cut(s) 367
EcoT14I CCWWGG 1 cut(s) 454
EcoT38I GRGCYC 1 cut(s) 104
ErhI CCWWGG 1 cut(s) 454
Esp3I CGTCTC 2 cut(s) 642, 770
FaeI CATG 7 cut(s) 391, 561, 647, 692, 794, 847, 887
FaqI GGGAC 2 cut(s) 831, 902
FatI CATG 7 cut(s) 387, 557, 643, 688, 790, 843, 883
FauI CCCGC 1 cut(s) 537
Fnu4HI GCNGC 1 cut(s) 657
FokI GGATG 1 cut(s) 72
FriOI GRGCYC 1 cut(s) 104
Fsp4HI GCNGC 1 cut(s) 657
GlaI GCGC 2 cut(s) 535, 543
GluI GCNGC 1 cut(s) 657
HaeIII GGCC 2 cut(s) 472, 686
HapII CCGG 1 cut(s) 306
HgaI GACGC 2 cut(s) 712, 734
HhaI GCGC 2 cut(s) 536, 544
Hin1II CATG 7 cut(s) 391, 561, 647, 692, 794, 847, 887
Hin6I GCGC 2 cut(s) 534, 542
HinP1I GCGC 2 cut(s) 534, 542
HinfI GANTC 2 cut(s) 853, 871
HpaII CCGG 1 cut(s) 306
HphI GGTGA 1 cut(s) 161
Hpy166II GTNNAC 2 cut(s) 303, 422
Hpy188I TCNGA 3 cut(s) 232, 523, 812
Hpy188III TCNNGA 1 cut(s) 271
Hpy8I GTNNAC 2 cut(s) 303, 422
Hpy99I CGWCG 4 cut(s) 239, 251, 706, 728
HpyAV CCTTC 4 cut(s) 62, 483, 815, 826
HpyCH4III ACNGT 5 cut(s) 224, 328, 419, 500, 781
HpyCH4IV ACGT 1 cut(s) 921
HpyCH4V TGCA 4 cut(s) 74, 659, 790, 883
HpyF10VI GCNNNNNNNGC 1 cut(s) 533
HpyF3I CTNAG 2 cut(s) 480, 826
HpySE526I ACGT 1 cut(s) 921
Hsp92II CATG 7 cut(s) 391, 561, 647, 692, 794, 847, 887
HspAI GCGC 2 cut(s) 534, 542
Kzo9I GATC 4 cut(s) 250, 282, 392, 621
LguI GCTCTTC 1 cut(s) 109
LmnI GCTCC 2 cut(s) 66, 836
Lsp1109I GCAGC 1 cut(s) 643
LweI GCATC 2 cut(s) 14, 174
MabI ACCWGGT 1 cut(s) 757
MaeII ACGT 1 cut(s) 921
MaeIII GTNAC 2 cut(s) 21, 599
MalI GATC 4 cut(s) 252, 284, 394, 623
MboI GATC 4 cut(s) 250, 282, 392, 621
MboII GAAGA 6 cut(s) 19, 96, 346, 497, 631, 848
MhlI GDGCHC 2 cut(s) 104, 804
MluCI AATT 2 cut(s) 242, 286
MlyI GAGTC 2 cut(s) 847, 865
MslI CAYNNNNRTG 1 cut(s) 147
MspI CCGG 1 cut(s) 306
MspR9I CCNGG 3 cut(s) 590, 672, 759
Mva1269I GAATGC 1 cut(s) 613
MvaI CCWGG 3 cut(s) 590, 672, 759
MvnI CGCG 2 cut(s) 536, 544
MwoI GCNNNNNNNGC 1 cut(s) 533
NdeII GATC 4 cut(s) 250, 282, 392, 621
NlaIII CATG 7 cut(s) 391, 561, 647, 692, 794, 847, 887
NlaIV GGNNCC 4 cut(s) 379, 410, 803, 849
NmuCI GTSAC 1 cut(s) 21
OliI CACNNNNGTG 1 cut(s) 147
PaeR7I CTCGAG 1 cut(s) 873
PciSI GCTCTTC 1 cut(s) 109
PcsI WCGNNNNNNNCGW 1 cut(s) 243
PctI GAATGC 1 cut(s) 613
PflFI GACNNNGTC 1 cut(s) 739
PkrI GCNGC 1 cut(s) 658
PleI GAGTC 2 cut(s) 847, 865
PpsI GAGTC 2 cut(s) 847, 865
Psp124BI GAGCTC 1 cut(s) 104
Psp6I CCWGG 3 cut(s) 588, 670, 757
PspGI CCWGG 3 cut(s) 588, 670, 757
PspN4I GGNNCC 4 cut(s) 379, 410, 803, 849
PspPI GGNCC 2 cut(s) 668, 685
PspXI VCTCGAGB 1 cut(s) 873
PsyI GACNNNGTC 1 cut(s) 739
RsaI GTAC 3 cut(s) 185, 203, 756
RsaNI GTAC 3 cut(s) 184, 202, 755
RseI CAYNNNNRTG 1 cut(s) 147
SacI GAGCTC 1 cut(s) 104
SapI GCTCTTC 1 cut(s) 109
SatI GCNGC 1 cut(s) 657
Sau3AI GATC 4 cut(s) 250, 282, 392, 621
Sau96I GGNCC 2 cut(s) 668, 685
SchI GAGTC 2 cut(s) 847, 865
ScrFI CCNGG 3 cut(s) 590, 672, 759
SduI GDGCHC 2 cut(s) 104, 804
SexAI ACCWGGT 1 cut(s) 757
SfaNI GCATC 2 cut(s) 14, 174
Sfr274I CTCGAG 1 cut(s) 873
SinI GGWCC 1 cut(s) 668
SlaI CTCGAG 1 cut(s) 873
SmiMI CAYNNNNRTG 1 cut(s) 147
SmlI CTYRAG 3 cut(s) 131, 296, 873
SmoI CTYRAG 3 cut(s) 131, 296, 873
Sse9I AATT 2 cut(s) 242, 286
SsiI CCGC 4 cut(s) 536, 544, 611, 675
SspI AATATT 1 cut(s) 714
SstI GAGCTC 1 cut(s) 104
StyD4I CCNGG 3 cut(s) 588, 670, 757
StyI CCWWGG 1 cut(s) 454
TaaI ACNGT 5 cut(s) 224, 328, 419, 500, 781
TaiI ACGT 1 cut(s) 924
TaqI TCGA 3 cut(s) 246, 332, 874
TasI AATT 2 cut(s) 242, 286
TscAI CASTG 2 cut(s) 333, 834
TseFI GTSAC 1 cut(s) 21
TseI GCWGC 1 cut(s) 656
Tsp45I GTSAC 1 cut(s) 21
TspDTI ATGAA 2 cut(s) 255, 416
TspGWI ACGGA 1 cut(s) 723
TspRI CASTG 2 cut(s) 333, 834
Tth111I GACNNNGTC 1 cut(s) 739
VpaK11BI GGWCC 1 cut(s) 668
XapI RAATTY 1 cut(s) 242
XcmI CCANNNNNNNNNTGG 2 cut(s) 310, 535
XhoI CTCGAG 1 cut(s) 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.