MD09G1040300.v1.1

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000309
Physical Location & Seq
Reverse (-)
2539729 .. 2540658
930 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1040300.v1.1.491

Sequence Viewer

Length: 930 bp
ATGGCATCAAAAAGTACTCTTTGCCTCTTCACAATCACCCTCGTCCTCGTCTTTCTTTCTGTTCTCTCCCATGCAACATCGGCAGAAACCCGCAACCAGAAACCTTCGCCGTTCGAGTTCCTTAACCATCTCAAGGGGTGTCACAAGGGTGACAAGGTCCAAGGCATCCAAGACCTAAAAAAGTACCTTGAAAAATTTGGATACTTGAACGGCCATTCCGATAGTGATGACTTCGACGACATCTTGGAGTCGGCGATCAAAACCTACCAAGTCAATTACCACCTCAAGGCCACCGGAACCTTAGATGCTAAAACAGTATCAAAAATGATAATGCCGCGATGTGGTGTCGCTGATATTATCAATGGTACAACCTCCATGAGATCCGGAAAGAAAAGGCACCCTCACCACCACGGCGGACATGGTTCGCCTCACACAGTTGCTCATTATTCTTTTTTCACCGGAAACCCAAAATGGCCTTCTTCTAAGTACCATCTCACCTATGCTTTTGATCAAAGCACCCCAACTGAGGCCCGGGATGCAGTTGCGCGCGCTTTCGCAACATGGGCAGGCAACACACACTTCTCGTTCAGTCAATCCCAAAACTATAAGAACGCGGATCTGACAATAAGTTTTGGTAAGGGTGATCATGGAGATGGGAACCCATTTGACGGCCGAGGTGGGATCCTTGCTCACGCATTTCCACCGACCAATGGGAGATTCCACTACGATGCCGATGAGGCGTGGGCTGTGGGAGCTGTGGCGGATGCTTATGACTTGGAGACTGTGGCCTTGCATGAAATCAGACATCTGTTGGGGCTACAACATAGCTCTGTTGAAGGAGCTGTCATGTCCCCTGGCGTTCGTAGTGGATTTACCCAAAGTTTGCATGCAGATGATATTCAAGGAATTAAAGCTTTGTATAACACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

310

Amino Acids

33.85

Weight (kDa)

7.07

Isoelectric Point (pI)

33.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 54 - 109 4.7e-12 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 157 - 307 1.1e-45 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 396
AccII CGCG 4 cut(s) 337, 547, 549, 614
AccIII TCCGGA 1 cut(s) 383
AciI CCGC 5 cut(s) 91, 335, 414, 614, 761
AclWI GGATC 4 cut(s) 375, 624, 676, 689
AcoI YGGCCR 2 cut(s) 211, 670
AcsI RAATTY 1 cut(s) 194
AfaI GTAC 4 cut(s) 16, 185, 367, 488
AfiI CCNNNNNNNGG 6 cut(s) 133, 286, 341, 526, 668, 710
AgsI TTSAA 4 cut(s) 191, 208, 836, 902
AjnI CCWGG 1 cut(s) 853
AjuI GAANNNNNNNTTGG 4 cut(s) 460, 492, 701, 733
AleI CACNNNNGTG 1 cut(s) 147
AloI GAACNNNNNNTCC 2 cut(s) 200, 232
AluBI AGCT 4 cut(s) 755, 828, 842, 914
AluI AGCT 4 cut(s) 755, 828, 842, 914
Alw26I GTCTC 1 cut(s) 773
AlwI GGATC 4 cut(s) 375, 624, 676, 689
Ama87I CYCGRG 1 cut(s) 531
Aor13HI TCCGGA 1 cut(s) 383
AoxI GGCC 6 cut(s) 211, 288, 473, 528, 670, 786
ApoI RAATTY 1 cut(s) 194
AspLEI GCGC 3 cut(s) 547, 549, 551
AspS9I GGNCC 2 cut(s) 157, 529
AsuC2I CCSGG 2 cut(s) 532, 533
AsuHPI GGTGA 6 cut(s) 28, 161, 395, 448, 487, 653
AvaI CYCGRG 1 cut(s) 531
AvaII GGWCC 1 cut(s) 157
BamHI GGATCC 1 cut(s) 681
BanI GGYRCC 1 cut(s) 396
BccI CCATC 3 cut(s) 135, 498, 647
BceAI ACGGC 4 cut(s) 94, 226, 427, 685
BciT130I CCWGG 1 cut(s) 855
BciVI GTATCC 1 cut(s) 194
BclI TGATCA 2 cut(s) 508, 643
BcnI CCSGG 2 cut(s) 532, 533
BcoDI GTCTC 1 cut(s) 773
BfuI GTATCC 1 cut(s) 194
BglI GCCNNNNNGGC 1 cut(s) 737
BisI GCNGC 1 cut(s) 335
BlsI GCNGC 1 cut(s) 336
BmcAI AGTACT 1 cut(s) 16
Bme1390I CCNGG 3 cut(s) 532, 533, 855
Bme18I GGWCC 1 cut(s) 157
BmeT110I CYCGRG 1 cut(s) 531
BmgT120I GGNCC 2 cut(s) 157, 529
BmiI GGNNCC 4 cut(s) 298, 398, 659, 683
BmrFI CCNGG 3 cut(s) 532, 533, 855
BmsI GCATC 6 cut(s) 14, 174, 295, 526, 718, 754
BpuEI CTTGAG 2 cut(s) 116, 269
BpuMI CCSGG 2 cut(s) 532, 533
BsaJI CCNNGG 5 cut(s) 160, 409, 531, 673, 853
BsaWI WCCGGW 3 cut(s) 293, 383, 458
Bsc4I CCNNNNNNNGG 6 cut(s) 133, 286, 341, 526, 668, 710
BseAI TCCGGA 1 cut(s) 383
BseBI CCWGG 1 cut(s) 855
BseDI CCNNGG 5 cut(s) 160, 409, 531, 673, 853
BseGI GGATG 3 cut(s) 165, 541, 769
BseLI CCNNNNNNNGG 6 cut(s) 133, 286, 341, 526, 668, 710
BseMII CTCAG 1 cut(s) 516
BsePI GCGCGC 2 cut(s) 545, 547
BseX3I CGGCCG 1 cut(s) 670
Bsh1236I CGCG 4 cut(s) 337, 547, 549, 614
Bsh1285I CGRYCG 1 cut(s) 673
BshFI GGCC 6 cut(s) 213, 290, 475, 530, 672, 788
BshNI GGYRCC 1 cut(s) 396
BsiEI CGRYCG 1 cut(s) 673
BsiHKCI CYCGRG 1 cut(s) 531
BsiSI CCGG 4 cut(s) 294, 384, 459, 532
BslFI GGGAC 1 cut(s) 835
BslI CCNNNNNNNGG 6 cut(s) 133, 286, 341, 526, 668, 710
BsmAI GTCTC 1 cut(s) 773
BsmFI GGGAC 1 cut(s) 835
BsnI GGCC 6 cut(s) 213, 290, 475, 530, 672, 788
BsoBI CYCGRG 1 cut(s) 531
Bsp13I TCCGGA 1 cut(s) 383
Bsp143I GATC 6 cut(s) 255, 380, 508, 616, 643, 681
BspACI CCGC 5 cut(s) 91, 335, 414, 614, 761
BspANI GGCC 6 cut(s) 213, 290, 475, 530, 672, 788
BspCNI CTCAG 1 cut(s) 517
BspEI TCCGGA 1 cut(s) 383
BspFNI CGCG 4 cut(s) 337, 547, 549, 614
BspLI GGNNCC 4 cut(s) 298, 398, 659, 683
BspPI GGATC 4 cut(s) 375, 624, 676, 689
BspT107I GGYRCC 1 cut(s) 396
BssECI CCNNGG 5 cut(s) 160, 409, 531, 673, 853
BssHII GCGCGC 2 cut(s) 545, 547
BssMI GATC 6 cut(s) 255, 380, 508, 616, 643, 681
BssT1I CCWWGG 1 cut(s) 160
Bst2UI CCWGG 1 cut(s) 855
Bst4CI ACNGT 3 cut(s) 316, 436, 784
Bst6I CTCTTC 1 cut(s) 32
BstC8I GCNNGC 4 cut(s) 547, 549, 568, 888
BstDEI CTNAG 3 cut(s) 301, 483, 525
BstDSI CCRYGG 1 cut(s) 409
BstF5I GGATG 3 cut(s) 165, 541, 769
BstFNI CGCG 4 cut(s) 337, 547, 549, 614
BstHHI GCGC 3 cut(s) 547, 549, 551
BstKTI GATC 6 cut(s) 258, 383, 511, 619, 646, 684
BstMAI GTCTC 1 cut(s) 773
BstMBI GATC 6 cut(s) 255, 380, 508, 616, 643, 681
BstMCI CGRYCG 1 cut(s) 673
BstMWI GCNNNNNNNGC 5 cut(s) 80, 536, 563, 737, 752
BstNI CCWGG 1 cut(s) 855
BstNSI RCATGY 1 cut(s) 890
BstSCI CCNGG 3 cut(s) 530, 531, 853
BstUI CGCG 4 cut(s) 337, 547, 549, 614
BstX2I RGATCY 3 cut(s) 380, 616, 681
BstYI RGATCY 3 cut(s) 380, 616, 681
BstZI CGGCCG 1 cut(s) 670
BsuI GTATCC 1 cut(s) 194
BsuRI GGCC 6 cut(s) 213, 290, 475, 530, 672, 788
BtgI CCRYGG 1 cut(s) 409
BtgZI GCGATG 1 cut(s) 352
BtsCI GGATG 3 cut(s) 165, 541, 769
Cac8I GCNNGC 4 cut(s) 547, 549, 568, 888
CfoI GCGC 3 cut(s) 547, 549, 551
Cfr13I GGNCC 2 cut(s) 157, 529
Cfr9I CCCGGG 1 cut(s) 531
Csp6I GTAC 4 cut(s) 15, 184, 366, 487
CviAII CATG 8 cut(s) 71, 376, 419, 561, 647, 794, 847, 887
CviQI GTAC 4 cut(s) 15, 184, 366, 487
DdeI CTNAG 3 cut(s) 301, 483, 525
DpnI GATC 6 cut(s) 257, 382, 510, 618, 645, 683
DpnII GATC 6 cut(s) 255, 380, 508, 616, 643, 681
EaeI YGGCCR 2 cut(s) 211, 670
EagI CGGCCG 1 cut(s) 670
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
EciI GGCGGA 2 cut(s) 429, 776
EclXI CGGCCG 1 cut(s) 670
Eco130I CCWWGG 1 cut(s) 160
Eco47I GGWCC 1 cut(s) 157
Eco52I CGGCCG 1 cut(s) 670
Eco88I CYCGRG 1 cut(s) 531
EcoRII CCWGG 1 cut(s) 853
EcoT14I CCWWGG 1 cut(s) 160
ErhI CCWWGG 1 cut(s) 160
FaeI CATG 8 cut(s) 74, 379, 422, 564, 650, 797, 850, 890
FaqI GGGAC 1 cut(s) 835
FatI CATG 8 cut(s) 70, 375, 418, 560, 646, 793, 846, 886
FauI CCCGC 1 cut(s) 98
FbaI TGATCA 2 cut(s) 508, 643
Fnu4HI GCNGC 1 cut(s) 335
FokI GGATG 3 cut(s) 152, 548, 776
Fsp4HI GCNGC 1 cut(s) 335
GlaI GCGC 3 cut(s) 546, 548, 550
GluI GCNGC 1 cut(s) 335
HaeIII GGCC 6 cut(s) 213, 290, 475, 530, 672, 788
HapII CCGG 4 cut(s) 294, 384, 459, 532
HhaI GCGC 3 cut(s) 547, 549, 551
Hin1II CATG 8 cut(s) 74, 379, 422, 564, 650, 797, 850, 890
Hin6I GCGC 3 cut(s) 545, 547, 549
HinP1I GCGC 3 cut(s) 545, 547, 549
HindIII AAGCTT 1 cut(s) 912
HinfI GANTC 2 cut(s) 248, 717
HpaII CCGG 4 cut(s) 294, 384, 459, 532
HphI GGTGA 6 cut(s) 28, 161, 395, 448, 487, 653
Hpy188I TCNGA 3 cut(s) 220, 621, 803
Hpy188III TCNNGA 1 cut(s) 384
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 3 cut(s) 114, 486, 830
HpyCH4III ACNGT 3 cut(s) 316, 436, 784
HpyCH4V TGCA 5 cut(s) 74, 539, 793, 886, 890
HpyF10VI GCNNNNNNNGC 5 cut(s) 80, 536, 563, 737, 752
HpyF3I CTNAG 3 cut(s) 301, 483, 525
Hsp92II CATG 8 cut(s) 74, 379, 422, 564, 650, 797, 850, 890
HspAI GCGC 3 cut(s) 545, 547, 549
Kpn2I TCCGGA 1 cut(s) 383
Ksp22I TGATCA 2 cut(s) 508, 643
Kzo9I GATC 6 cut(s) 255, 380, 508, 616, 643, 681
LmnI GCTCC 2 cut(s) 752, 839
LpnPI CCDG 8 cut(s) 110, 307, 397, 472, 545, 552, 840, 867
LweI GCATC 6 cut(s) 14, 174, 295, 526, 718, 754
MaeIII GTNAC 2 cut(s) 140, 149
MalI GATC 6 cut(s) 257, 382, 510, 618, 645, 683
MboI GATC 6 cut(s) 255, 380, 508, 616, 643, 681
MboII GAAGA 2 cut(s) 19, 471
MflI RGATCY 3 cut(s) 380, 616, 681
MluCI AATT 3 cut(s) 194, 274, 906
MlyI GAGTC 1 cut(s) 257
MroI TCCGGA 1 cut(s) 383
MseI TTAA 2 cut(s) 123, 909
MslI CAYNNNNRTG 4 cut(s) 147, 651, 726, 891
MspI CCGG 4 cut(s) 294, 384, 459, 532
MspR9I CCNGG 3 cut(s) 532, 533, 855
MvaI CCWGG 1 cut(s) 855
MvnI CGCG 4 cut(s) 337, 547, 549, 614
MwoI GCNNNNNNNGC 5 cut(s) 80, 536, 563, 737, 752
NciI CCSGG 2 cut(s) 532, 533
NdeII GATC 6 cut(s) 255, 380, 508, 616, 643, 681
NlaIII CATG 8 cut(s) 74, 379, 422, 564, 650, 797, 850, 890
NlaIV GGNNCC 4 cut(s) 298, 398, 659, 683
NmeAIII GCCGAG 1 cut(s) 698
NmuCI GTSAC 2 cut(s) 140, 149
NspI RCATGY 1 cut(s) 890
OliI CACNNNNGTG 1 cut(s) 147
PaeI GCATGC 1 cut(s) 890
PauI GCGCGC 2 cut(s) 545, 547
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PfeI GAWTC 1 cut(s) 717
PflFI GACNNNGTC 1 cut(s) 155
PkrI GCNGC 1 cut(s) 336
PleI GAGTC 1 cut(s) 256
PpsI GAGTC 1 cut(s) 256
Psp6I CCWGG 1 cut(s) 853
PspGI CCWGG 1 cut(s) 853
PspN4I GGNNCC 4 cut(s) 298, 398, 659, 683
PspPI GGNCC 2 cut(s) 157, 529
PsuI RGATCY 3 cut(s) 380, 616, 681
PsyI GACNNNGTC 1 cut(s) 155
PteI GCGCGC 2 cut(s) 545, 547
RsaI GTAC 4 cut(s) 16, 185, 367, 488
RsaNI GTAC 4 cut(s) 15, 184, 366, 487
RseI CAYNNNNRTG 4 cut(s) 147, 651, 726, 891
SaqAI TTAA 2 cut(s) 123, 909
SatI GCNGC 1 cut(s) 335
Sau3AI GATC 6 cut(s) 255, 380, 508, 616, 643, 681
Sau96I GGNCC 2 cut(s) 157, 529
ScaI AGTACT 1 cut(s) 16
SchI GAGTC 1 cut(s) 257
ScrFI CCNGG 3 cut(s) 532, 533, 855
SfaNI GCATC 6 cut(s) 14, 174, 295, 526, 718, 754
SinI GGWCC 1 cut(s) 157
SmaI CCCGGG 1 cut(s) 533
SmiMI CAYNNNNRTG 4 cut(s) 147, 651, 726, 891
SmlI CTYRAG 2 cut(s) 131, 284
SmoI CTYRAG 2 cut(s) 131, 284
SphI GCATGC 1 cut(s) 890
Sse9I AATT 3 cut(s) 194, 274, 906
SsiI CCGC 5 cut(s) 91, 335, 414, 614, 761
StyD4I CCNGG 3 cut(s) 530, 531, 853
StyI CCWWGG 1 cut(s) 160
TaaI ACNGT 3 cut(s) 316, 436, 784
TaqI TCGA 2 cut(s) 114, 234
TasI AATT 3 cut(s) 194, 274, 906
TatI WGTACW 1 cut(s) 14
TauI GCSGC 1 cut(s) 337
TfiI GAWTC 1 cut(s) 717
Tru1I TTAA 2 cut(s) 123, 909
Tru9I TTAA 2 cut(s) 123, 909
TseFI GTSAC 2 cut(s) 140, 149
Tsp45I GTSAC 2 cut(s) 140, 149
TspDTI ATGAA 1 cut(s) 810
TspMI CCCGGG 1 cut(s) 531
Tth111I GACNNNGTC 1 cut(s) 155
VpaK11BI GGWCC 1 cut(s) 157
XapI RAATTY 1 cut(s) 194
XceI RCATGY 1 cut(s) 890
XcmI CCANNNNNNNNNTGG 1 cut(s) 416
XmaI CCCGGG 1 cut(s) 531
ZrmI AGTACT 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.