Rroxscaffold_2G00081470

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000309
Physical Location & Seq
Forward (+)
4503237 .. 4504138
902 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00081470.1

Sequence Viewer

Length: 639 bp
ATGGCATCCAAATCTAATCTTTCTCTCTTCACTGTCACTCTCCTCCTTGCCCTTCTTGCTCTCCTCTCTTATGCAACTGCTTCTCATCCCCACAACAAGAGCTCTTCCCCATTTGAGTTCCTTGAGCATCTCAAGGGTTGCCACAAAGGTGATAAGGTCAAGGGCATCAATGATCTCAAAAAATACCTAGAAAAGTACGGTTACTTGAACTACAAGAACTACAGTCATTCGGACGACGATGATTTTGATGACCTTTTGGAGGAAGCCATCAAGACATACCAGGTCAATTACCACCTCAAGTCCACCGGAACATTGGATGGCAAAACAGTGTCGAAGATGATGACACCTCGTTGTGGCGTGCCGGATATCATCAATGGCACCTCGTCCATGAGATCAGCCAAGAAAAGGCACCACCATGTACTCGGTATTGTTTTACAGGTTCAATTCATACCACTGCTCACTACTCATTTTTCCAGGGAAATCCAAAATGGCCTTCTTCTAAATACCATCTCACTTACGGTTTTCTCCCCAACACCCCATCCGAAGCCACGGGCGCTGTCGCACGGGCTTTCGCAACATGGGCTGGAAACACTCACTTCACCTTCAGCCAGTCCCAAAGTTACGAGAATGCGGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

212

Amino Acids

23.64

Weight (kDa)

9.43

Isoelectric Point (pI)

26.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 56 - 113 1.5e-10 Putative peptidoglycan binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 377, 408
AciI CCGC 1 cut(s) 631
AcuI CTGAAG 1 cut(s) 588
AfaI GTAC 2 cut(s) 197, 420
AfiI CCNNNNNNNGG 3 cut(s) 259, 353, 405
AgsI TTSAA 2 cut(s) 208, 443
AjnI CCWGG 2 cut(s) 279, 473
AjuI GAANNNNNNNTTGG 2 cut(s) 477, 509
AleI CACNNNNGTG 1 cut(s) 147
AluBI AGCT 1 cut(s) 102
AluI AGCT 1 cut(s) 102
Alw21I GWGCWC 1 cut(s) 104
AoxI GGCC 1 cut(s) 490
ArsI GACNNNNNNTTYG 2 cut(s) 227, 259
AspLEI GCGC 1 cut(s) 556
AsuHPI GGTGA 2 cut(s) 161, 591
BanI GGYRCC 2 cut(s) 377, 408
BanII GRGCYC 1 cut(s) 104
Bbv12I GWGCWC 1 cut(s) 104
BccI CCATC 4 cut(s) 275, 311, 515, 546
BciT130I CCWGG 2 cut(s) 281, 475
BfaI CTAG 1 cut(s) 188
BfmI CTRYAG 1 cut(s) 220
BfoI RGCGCY 1 cut(s) 557
Bme1390I CCNGG 2 cut(s) 281, 475
BmiI GGNNCC 2 cut(s) 379, 410
BmrFI CCNGG 2 cut(s) 281, 475
BmsI GCATC 3 cut(s) 14, 136, 174
BpuEI CTTGAG 3 cut(s) 116, 143, 281
BsaJI CCNNGG 2 cut(s) 474, 548
BsaWI WCCGGW 1 cut(s) 305
Bsc4I CCNNNNNNNGG 3 cut(s) 259, 353, 405
Bse1I ACTGG 1 cut(s) 609
BseBI CCWGG 2 cut(s) 281, 475
BseDI CCNNGG 2 cut(s) 474, 548
BseGI GGATG 4 cut(s) 5, 85, 322, 538
BseLI CCNNNNNNNGG 3 cut(s) 259, 353, 405
BseNI ACTGG 1 cut(s) 609
BseRI GAGGAG 2 cut(s) 32, 53
BshFI GGCC 1 cut(s) 492
BshNI GGYRCC 2 cut(s) 377, 408
BsiHKAI GWGCWC 1 cut(s) 104
BsiSI CCGG 2 cut(s) 306, 362
BslFI GGGAC 1 cut(s) 597
BslI CCNNNNNNNGG 3 cut(s) 259, 353, 405
BsmFI GGGAC 1 cut(s) 597
BsmI GAATGC 1 cut(s) 633
BsnI GGCC 1 cut(s) 492
Bsp1286I GDGCHC 1 cut(s) 104
Bsp143I GATC 2 cut(s) 172, 392
BspACI CCGC 1 cut(s) 631
BspANI GGCC 1 cut(s) 492
BspLI GGNNCC 2 cut(s) 379, 410
BspQI GCTCTTC 1 cut(s) 109
BspT107I GGYRCC 2 cut(s) 377, 408
BsrI ACTGG 1 cut(s) 609
BssECI CCNNGG 2 cut(s) 474, 548
BssMI GATC 2 cut(s) 172, 392
Bst2UI CCWGG 2 cut(s) 281, 475
Bst4CI ACNGT 5 cut(s) 34, 200, 224, 328, 520
Bst6I CTCTTC 2 cut(s) 32, 109
BstC8I GCNNGC 1 cut(s) 359
BstDSI CCRYGG 1 cut(s) 548
BstENI CCTNNNNNAGG 1 cut(s) 257
BstF5I GGATG 4 cut(s) 5, 85, 322, 538
BstH2I RGCGCY 1 cut(s) 557
BstHHI GCGC 1 cut(s) 556
BstKTI GATC 2 cut(s) 175, 395
BstMBI GATC 2 cut(s) 172, 392
BstMWI GCNNNNNNNGC 3 cut(s) 56, 553, 580
BstNI CCWGG 2 cut(s) 281, 475
BstSCI CCNGG 2 cut(s) 279, 473
BstSFI CTRYAG 1 cut(s) 220
BsuRI GGCC 1 cut(s) 492
BtgI CCRYGG 1 cut(s) 548
BtsCI GGATG 4 cut(s) 5, 85, 322, 538
BtsI GCAGTG 1 cut(s) 452
BtsIMutI CAGTG 3 cut(s) 30, 333, 452
Cac8I GCNNGC 1 cut(s) 359
CfoI GCGC 1 cut(s) 556
CsiI ACCWGGT 1 cut(s) 279
Csp6I GTAC 2 cut(s) 196, 419
CviAII CATG 3 cut(s) 388, 416, 578
CviJI RGCY 8 cut(s) 102, 266, 398, 492, 547, 568, 583, 608
CviKI_1 RGCY 8 cut(s) 102, 266, 398, 492, 547, 568, 583, 608
CviQI GTAC 2 cut(s) 196, 419
DpnI GATC 2 cut(s) 174, 394
DpnII GATC 2 cut(s) 172, 392
Eam1104I CTCTTC 2 cut(s) 32, 109
EarI CTCTTC 2 cut(s) 32, 109
Ecl136II GAGCTC 1 cut(s) 102
Eco24I GRGCYC 1 cut(s) 104
Eco32I GATATC 1 cut(s) 367
Eco53kI GAGCTC 1 cut(s) 102
Eco57I CTGAAG 1 cut(s) 588
EcoICRI GAGCTC 1 cut(s) 102
EcoNI CCTNNNNNAGG 1 cut(s) 257
EcoRII CCWGG 2 cut(s) 279, 473
EcoRV GATATC 1 cut(s) 367
EcoT38I GRGCYC 1 cut(s) 104
FaeI CATG 3 cut(s) 391, 419, 581
FaiI YATR 6 cut(s) 72, 277, 389, 417, 449, 579
FaqI GGGAC 1 cut(s) 597
FatI CATG 3 cut(s) 387, 415, 577
FokI GGATG 3 cut(s) 72, 329, 525
FriOI GRGCYC 1 cut(s) 104
FspBI CTAG 1 cut(s) 188
GlaI GCGC 1 cut(s) 555
HaeII RGCGCY 1 cut(s) 557
HaeIII GGCC 1 cut(s) 492
HapII CCGG 2 cut(s) 306, 362
HhaI GCGC 1 cut(s) 556
Hin1II CATG 3 cut(s) 391, 419, 581
Hin6I GCGC 1 cut(s) 554
HinP1I GCGC 1 cut(s) 554
HpaII CCGG 2 cut(s) 306, 362
HphI GGTGA 2 cut(s) 161, 591
Hpy166II GTNNAC 1 cut(s) 303
Hpy188I TCNGA 2 cut(s) 232, 543
Hpy188III TCNNGA 1 cut(s) 271
Hpy8I GTNNAC 1 cut(s) 303
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 3 cut(s) 62, 503, 612
HpyCH4III ACNGT 5 cut(s) 34, 200, 224, 328, 520
HpyCH4V TGCA 1 cut(s) 74
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 553, 580
Hsp92II CATG 3 cut(s) 391, 419, 581
HspAI GCGC 1 cut(s) 554
Kzo9I GATC 2 cut(s) 172, 392
LguI GCTCTTC 1 cut(s) 109
LpnPI CCDG 9 cut(s) 266, 293, 319, 375, 422, 460, 487, 569, 622
LweI GCATC 3 cut(s) 14, 136, 174
MabI ACCWGGT 1 cut(s) 279
MaeI CTAG 1 cut(s) 188
MaeIII GTNAC 3 cut(s) 34, 200, 619
MalI GATC 2 cut(s) 174, 394
MboI GATC 2 cut(s) 172, 392
MboII GAAGA 4 cut(s) 19, 96, 346, 488
MhlI GDGCHC 1 cut(s) 104
MluCI AATT 2 cut(s) 286, 443
MnlI CCTC 6 cut(s) 53, 74, 253, 305, 357, 391
MslI CAYNNNNRTG 2 cut(s) 147, 414
MspI CCGG 2 cut(s) 306, 362
MspR9I CCNGG 2 cut(s) 281, 475
Mva1269I GAATGC 1 cut(s) 633
MvaI CCWGG 2 cut(s) 281, 475
MwoI GCNNNNNNNGC 3 cut(s) 56, 553, 580
NdeII GATC 2 cut(s) 172, 392
NlaIII CATG 3 cut(s) 391, 419, 581
NlaIV GGNNCC 2 cut(s) 379, 410
NmuCI GTSAC 1 cut(s) 34
OliI CACNNNNGTG 1 cut(s) 147
PciSI GCTCTTC 1 cut(s) 109
PctI GAATGC 1 cut(s) 633
Psp124BI GAGCTC 1 cut(s) 104
Psp6I CCWGG 2 cut(s) 279, 473
PspGI CCWGG 2 cut(s) 279, 473
PspN4I GGNNCC 2 cut(s) 379, 410
RsaI GTAC 2 cut(s) 197, 420
RsaNI GTAC 2 cut(s) 196, 419
RseI CAYNNNNRTG 2 cut(s) 147, 414
SacI GAGCTC 1 cut(s) 104
SapI GCTCTTC 1 cut(s) 109
Sau3AI GATC 2 cut(s) 172, 392
ScrFI CCNGG 2 cut(s) 281, 475
SduI GDGCHC 1 cut(s) 104
SexAI ACCWGGT 1 cut(s) 279
SfaNI GCATC 3 cut(s) 14, 136, 174
SfcI CTRYAG 1 cut(s) 220
SmiMI CAYNNNNRTG 2 cut(s) 147, 414
SmlI CTYRAG 3 cut(s) 122, 131, 296
SmoI CTYRAG 3 cut(s) 122, 131, 296
Sse9I AATT 2 cut(s) 286, 443
SsiI CCGC 1 cut(s) 631
SspMI CTAG 1 cut(s) 188
SstI GAGCTC 1 cut(s) 104
StyD4I CCNGG 2 cut(s) 279, 473
TaaI ACNGT 5 cut(s) 34, 200, 224, 328, 520
TaqI TCGA 1 cut(s) 332
TasI AATT 2 cut(s) 286, 443
TatI WGTACW 1 cut(s) 418
TscAI CASTG 3 cut(s) 37, 333, 459
TseFI GTSAC 1 cut(s) 34
Tsp45I GTSAC 1 cut(s) 34
TspDTI ATGAA 1 cut(s) 436
TspRI CASTG 3 cut(s) 37, 333, 459
XagI CCTNNNNNAGG 1 cut(s) 257
XcmI CCANNNNNNNNNTGG 1 cut(s) 310
XspI CTAG 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.