Rh2BG644800

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000309
Physical Location & Seq
Reverse (-)
86472647 .. 86478045
5399 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG644800.1

Sequence Viewer

Length: 495 bp
ATGGCACCAAAATCTGATCTTTCTCTTTTCACAGTCACTCTCCTCCTCCTCCTCGCCCTCTTTTCTTTCCTCTCTAATGCAATGGCAAACTCATCTCCGTTTCAGTTCCTTGAGCATCTTAAGGGTTGTCACAAAGGTGACAAGGTCAAGGGCATCAATGACCTCAAGAATTACCTTCACAACTTCGGTTACTTGAACTACAAGAACCACATCCATTCTGATGACGATGATTTCGACGAGCTCTTGGAGGAAGCCGTCAAGACTTACCAGCTCAATGTCCACCTCAAGTCCACCGGAACGTTGGACGACAAAACCATATCACAGATGATGATGCCTCGTTGTGGGTTAAACATCCCCTTTCCAGTGAATTACAATGATATTGGATATGATTATATCAACAGACGCGTAGAAATGAAGCCGCTTTTCCATGCCATTGCATTGCAGCGGCACATACTTTATGCACACAGGTACTTGGGATGCAGTTGTGACTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

164

Amino Acids

18.87

Weight (kDa)

6.48

Isoelectric Point (pI)

42.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 52 - 109 1.1e-09 Putative peptidoglycan binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AccII CGCG 1 cut(s) 405
AciI CCGC 2 cut(s) 419, 445
AclI AACGTT 1 cut(s) 299
AfaI GTAC 1 cut(s) 470
AfiI CCNNNNNNNGG 1 cut(s) 341
AflII CTTAAG 1 cut(s) 119
AflIII ACRYGT 1 cut(s) 403
AgsI TTSAA 1 cut(s) 196
AleI CACNNNNGTG 1 cut(s) 135
AluBI AGCT 2 cut(s) 241, 271
AluI AGCT 2 cut(s) 241, 271
Alw21I GWGCWC 1 cut(s) 243
ApeKI GCWGC 1 cut(s) 442
ArsI GACNNNNNNTTYG 2 cut(s) 215, 247
AsuHPI GGTGA 1 cut(s) 149
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 243
Bbv12I GWGCWC 1 cut(s) 243
BbvI GCAGC 1 cut(s) 454
BceAI ACGGC 1 cut(s) 239
BfrI CTTAAG 1 cut(s) 119
BisI GCNGC 3 cut(s) 419, 443, 446
BlsI GCNGC 3 cut(s) 420, 444, 447
BmiI GGNNCC 1 cut(s) 6
BmsI GCATC 4 cut(s) 124, 162, 321, 467
BpuEI CTTGAG 3 cut(s) 131, 149, 269
BsaWI WCCGGW 1 cut(s) 293
Bsc4I CCNNNNNNNGG 1 cut(s) 341
Bse1I ACTGG 1 cut(s) 362
Bse3DI GCAATG 3 cut(s) 87, 432, 437
BseGI GGATG 3 cut(s) 210, 351, 482
BseLI CCNNNNNNNGG 1 cut(s) 341
BseMI GCAATG 3 cut(s) 87, 432, 437
BseNI ACTGG 1 cut(s) 362
BseRI GAGGAG 4 cut(s) 32, 35, 38, 41
BseXI GCAGC 1 cut(s) 454
Bsh1236I CGCG 1 cut(s) 405
BshNI GGYRCC 1 cut(s) 4
BsiHKAI GWGCWC 1 cut(s) 243
BsiSI CCGG 1 cut(s) 294
BslI CCNNNNNNNGG 1 cut(s) 341
Bsp1286I GDGCHC 1 cut(s) 243
Bsp143I GATC 1 cut(s) 16
BspACI CCGC 2 cut(s) 419, 445
BspFNI CGCG 1 cut(s) 405
BspLI GGNNCC 1 cut(s) 6
BspT107I GGYRCC 1 cut(s) 4
BspTI CTTAAG 1 cut(s) 119
BsrDI GCAATG 3 cut(s) 87, 432, 437
BsrI ACTGG 1 cut(s) 362
BssMI GATC 1 cut(s) 16
Bst4CI ACNGT 1 cut(s) 34
BstAFI CTTAAG 1 cut(s) 119
BstF5I GGATG 3 cut(s) 210, 351, 482
BstFNI CGCG 1 cut(s) 405
BstKTI GATC 1 cut(s) 19
BstMBI GATC 1 cut(s) 16
BstUI CGCG 1 cut(s) 405
BstV1I GCAGC 1 cut(s) 454
BtsCI GGATG 3 cut(s) 210, 351, 482
BtsIMutI CAGTG 1 cut(s) 369
CseI GACGC 1 cut(s) 411
Csp6I GTAC 1 cut(s) 469
CviAII CATG 1 cut(s) 428
CviJI RGCY 4 cut(s) 241, 254, 271, 418
CviKI_1 RGCY 4 cut(s) 241, 254, 271, 418
CviQI GTAC 1 cut(s) 469
DpnI GATC 1 cut(s) 18
DpnII GATC 1 cut(s) 16
Ecl136II GAGCTC 1 cut(s) 241
Eco24I GRGCYC 1 cut(s) 243
Eco53kI GAGCTC 1 cut(s) 241
EcoICRI GAGCTC 1 cut(s) 241
EcoT38I GRGCYC 1 cut(s) 243
FaeI CATG 1 cut(s) 431
FaiI YATR 6 cut(s) 317, 387, 393, 429, 452, 459
FatI CATG 1 cut(s) 427
Fnu4HI GCNGC 3 cut(s) 419, 443, 446
FokI GGATG 3 cut(s) 197, 338, 489
FriOI GRGCYC 1 cut(s) 243
Fsp4HI GCNGC 3 cut(s) 419, 443, 446
GluI GCNGC 3 cut(s) 419, 443, 446
HapII CCGG 1 cut(s) 294
HgaI GACGC 1 cut(s) 411
Hin1II CATG 1 cut(s) 431
HpaII CCGG 1 cut(s) 294
HphI GGTGA 1 cut(s) 149
Hpy166II GTNNAC 2 cut(s) 280, 291
Hpy188I TCNGA 2 cut(s) 16, 220
Hpy188III TCNNGA 2 cut(s) 166, 259
Hpy8I GTNNAC 2 cut(s) 280, 291
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 1 cut(s) 185
HpyCH4III ACNGT 1 cut(s) 34
HpyCH4IV ACGT 1 cut(s) 299
HpyCH4V TGCA 5 cut(s) 80, 437, 442, 461, 480
HpySE526I ACGT 1 cut(s) 299
Hsp92II CATG 1 cut(s) 431
Kzo9I GATC 1 cut(s) 16
LpnPI CCDG 4 cut(s) 281, 307, 375, 451
Lsp1109I GCAGC 1 cut(s) 454
LweI GCATC 4 cut(s) 124, 162, 321, 467
MaeII ACGT 1 cut(s) 299
MaeIII GTNAC 5 cut(s) 34, 128, 137, 188, 485
MalI GATC 1 cut(s) 18
MboI GATC 1 cut(s) 16
MhlI GDGCHC 1 cut(s) 243
MluCI AATT 2 cut(s) 169, 367
MluI ACGCGT 1 cut(s) 403
MmeI TCCRAC 1 cut(s) 282
MseI TTAA 2 cut(s) 120, 347
MslI CAYNNNNRTG 2 cut(s) 135, 219
MspA1I CMGCKG 1 cut(s) 445
MspCI CTTAAG 1 cut(s) 119
MspI CCGG 1 cut(s) 294
MvnI CGCG 1 cut(s) 405
NdeII GATC 1 cut(s) 16
NlaIII CATG 1 cut(s) 431
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 4 cut(s) 34, 128, 137, 485
OliI CACNNNNGTG 1 cut(s) 135
PcsI WCGNNNNNNNCGW 1 cut(s) 231
PflFI GACNNNGTC 1 cut(s) 143
PkrI GCNGC 3 cut(s) 420, 444, 447
Psp124BI GAGCTC 1 cut(s) 243
Psp1406I AACGTT 1 cut(s) 299
PspN4I GGNNCC 1 cut(s) 6
PsyI GACNNNGTC 1 cut(s) 143
RsaI GTAC 1 cut(s) 470
RsaNI GTAC 1 cut(s) 469
RseI CAYNNNNRTG 2 cut(s) 135, 219
SacI GAGCTC 1 cut(s) 243
SaqAI TTAA 2 cut(s) 120, 347
SatI GCNGC 3 cut(s) 419, 443, 446
Sau3AI GATC 1 cut(s) 16
SduI GDGCHC 1 cut(s) 243
SetI ASST 9 cut(s) 139, 147, 165, 177, 243, 273, 285, 302, 470
SfaNI GCATC 4 cut(s) 124, 162, 321, 467
SmiMI CAYNNNNRTG 2 cut(s) 135, 219
SmlI CTYRAG 4 cut(s) 110, 119, 164, 284
SmoI CTYRAG 4 cut(s) 110, 119, 164, 284
Sse9I AATT 2 cut(s) 169, 367
SsiI CCGC 2 cut(s) 419, 445
SstI GAGCTC 1 cut(s) 243
TaaI ACNGT 1 cut(s) 34
TaiI ACGT 1 cut(s) 302
TaqI TCGA 1 cut(s) 234
TasI AATT 2 cut(s) 169, 367
TauI GCSGC 2 cut(s) 421, 448
Tru1I TTAA 2 cut(s) 120, 347
Tru9I TTAA 2 cut(s) 120, 347
TscAI CASTG 1 cut(s) 369
TseFI GTSAC 4 cut(s) 34, 128, 137, 485
TseI GCWGC 1 cut(s) 442
Tsp45I GTSAC 4 cut(s) 34, 128, 137, 485
TspDTI ATGAA 1 cut(s) 428
TspGWI ACGGA 1 cut(s) 87
TspRI CASTG 1 cut(s) 369
Tth111I GACNNNGTC 1 cut(s) 143
Vha464I CTTAAG 1 cut(s) 119
XcmI CCANNNNNNNNNTGG 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.