pycom17g03720

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0000309
Physical Location & Seq
Forward (+)
2531888 .. 2532875
988 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g03720.1

Sequence Viewer

Length: 897 bp
ATGCAAAGCTTTTGCAACATCCTGAATCTGCTTAATCATAAGTGCTTTACTAGTATAATCAATGGCATCACAAAACACCCTTTCTCTCTTCACAGTCACTCTCCTCGTCCTCCTTTCTCTCCTCTCCCAAGCATCCAAGACCTAAAAAAGTACCTTGAAAAATTTGGATATTTGAATAGCTATTCCGATAATGATGACTTCGACGACATCTTGGAGTCGGCGATCAAAACCTACCAAGTCAATTACCACCTCAAGGCTACCGGAACATTGGATGCCAAAACAGTGTCGAGAATGACGATGCCACGGTGTGGTGTCCCAGATATCATTAATGGTACCACCTCCATGCGATCGGGAAAGAAAAGGCAACCTCATCACCGTGGCGGACATGTTTCGACTCACACAGTTGCTCATTTTTCTTTCTTCTCCGGAAACCCTAAATGGCCTTCGTCTAAGTACCAACTCACCTATGCTTTTGATCAAAGCACCCCAGCTGAGGCTCAGGCCGCAGTTGCACGAGCTTTTGCAACATGGGCAGGAAACACACACTTCTCGTTCAGTCAAGCCCAAAGCTACGAAAACGCGGATCTGAAGATCAGTTTTGGCAGGGGAGATCATGGAGATGGGAGCCCATTTGACGGCCGAGGTGGGACTCTTGCTCATGCATCTCCACCAACCAATGGGATATTCCACTACGACGCCGATGAGCCGTGGGCTGTGGGGGCTGTGGAGGGTGCTTATGACTTGGAAACCGTGGCTTTGCATGAAATCGGGCATCTGCTTGGGCTAGGACATAGCTCCGTTGAGGGAGCTGTCATGTCTTCGAGTGTTCGTACTGGATATACCCAAAGTTTGCATGCGGATGATATTCAAGGAATTAAAGCTTTATATAACACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

299

Amino Acids

32.59

Weight (kDa)

6.64

Isoelectric Point (pI)

34.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 44 - 98 2.3e-11 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 146 - 296 8.7e-46 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 332
AccB1I GGYRCC 1 cut(s) 332
AccB7I CCANNNNNTGG 2 cut(s) 308, 677
AccII CGCG 1 cut(s) 581
AccIII TCCGGA 1 cut(s) 425
AciI CCGC 4 cut(s) 381, 504, 581, 857
AclWI GGATC 1 cut(s) 591
AcoI YGGCCR 1 cut(s) 637
AcsI RAATTY 1 cut(s) 161
AcuI CTGAAG 1 cut(s) 608
AcyI GRCGYC 1 cut(s) 696
AdeI CACNNNGTG 1 cut(s) 308
AfaI GTAC 4 cut(s) 152, 334, 455, 832
AfiI CCNNNNNNNGG 5 cut(s) 253, 308, 493, 635, 677
AflIII ACRYGT 1 cut(s) 385
AgsI TTSAA 3 cut(s) 158, 175, 869
AhlI ACTAGT 1 cut(s) 50
AjuI GAANNNNNNNTTGG 2 cut(s) 668, 700
AluBI AGCT 8 cut(s) 9, 180, 491, 518, 570, 795, 809, 881
AluI AGCT 8 cut(s) 9, 180, 491, 518, 570, 795, 809, 881
AlwI GGATC 1 cut(s) 591
Aor13HI TCCGGA 1 cut(s) 425
AoxI GGCC 3 cut(s) 440, 501, 637
ApoI RAATTY 1 cut(s) 161
AseI ATTAAT 1 cut(s) 327
Asp718I GGTACC 1 cut(s) 332
AsuHPI GGTGA 2 cut(s) 365, 454
BanI GGYRCC 1 cut(s) 332
BanII GRGCYC 1 cut(s) 629
BauI CACGAG 1 cut(s) 513
BbsI GAAGAC 1 cut(s) 810
BbvCI CCTCAGC 1 cut(s) 492
BccI CCATC 1 cut(s) 614
BceAI ACGGC 2 cut(s) 652, 691
BclI TGATCA 1 cut(s) 475
BcuI ACTAGT 1 cut(s) 50
BfaI CTAG 2 cut(s) 51, 785
BisI GCNGC 1 cut(s) 504
BlsI GCNGC 1 cut(s) 505
BmiI GGNNCC 2 cut(s) 334, 626
BmsI GCATC 6 cut(s) 75, 141, 262, 288, 671, 781
BpiI GAAGAC 1 cut(s) 810
Bpu10I CCTNAGC 2 cut(s) 492, 498
BpuEI CTTGAG 1 cut(s) 236
BsaHI GRCGYC 1 cut(s) 696
BsaJI CCNNGG 5 cut(s) 302, 376, 640, 707, 750
BsaWI WCCGGW 2 cut(s) 260, 425
Bsc4I CCNNNNNNNGG 5 cut(s) 253, 308, 493, 635, 677
Bse1I ACTGG 1 cut(s) 838
BseAI TCCGGA 1 cut(s) 425
BseDI CCNNGG 5 cut(s) 302, 376, 640, 707, 750
BseGI GGATG 4 cut(s) 18, 132, 277, 865
BseLI CCNNNNNNNGG 5 cut(s) 253, 308, 493, 635, 677
BseMII CTCAG 2 cut(s) 483, 512
BseNI ACTGG 1 cut(s) 838
BseRI GAGGAG 2 cut(s) 93, 111
BseX3I CGGCCG 1 cut(s) 637
BseYI CCCAGC 1 cut(s) 487
Bsh1236I CGCG 1 cut(s) 581
Bsh1285I CGRYCG 2 cut(s) 350, 640
BshFI GGCC 3 cut(s) 442, 503, 639
BshNI GGYRCC 1 cut(s) 332
BsiEI CGRYCG 2 cut(s) 350, 640
BsiSI CCGG 2 cut(s) 261, 426
BslFI GGGAC 2 cut(s) 299, 661
BslI CCNNNNNNNGG 5 cut(s) 253, 308, 493, 635, 677
BsmFI GGGAC 2 cut(s) 299, 661
BsnI GGCC 3 cut(s) 442, 503, 639
Bsp1286I GDGCHC 1 cut(s) 629
Bsp13I TCCGGA 1 cut(s) 425
Bsp143I GATC 6 cut(s) 222, 347, 475, 583, 591, 610
BspACI CCGC 4 cut(s) 381, 504, 581, 857
BspANI GGCC 3 cut(s) 442, 503, 639
BspCNI CTCAG 2 cut(s) 484, 511
BspEI TCCGGA 1 cut(s) 425
BspFNI CGCG 1 cut(s) 581
BspLI GGNNCC 2 cut(s) 334, 626
BspPI GGATC 1 cut(s) 591
BspT107I GGYRCC 1 cut(s) 332
BsrI ACTGG 1 cut(s) 838
BssECI CCNNGG 5 cut(s) 302, 376, 640, 707, 750
BssMI GATC 6 cut(s) 222, 347, 475, 583, 591, 610
BssNI GRCGYC 1 cut(s) 696
BssSI CACGAG 1 cut(s) 513
Bst2BI CACGAG 1 cut(s) 513
Bst4CI ACNGT 6 cut(s) 95, 283, 306, 377, 403, 751
Bst6I CTCTTC 1 cut(s) 93
BstACI GRCGYC 1 cut(s) 696
BstC8I GCNNGC 1 cut(s) 855
BstDEI CTNAG 3 cut(s) 450, 492, 498
BstDSI CCRYGG 4 cut(s) 302, 376, 707, 750
BstF5I GGATG 4 cut(s) 18, 132, 277, 865
BstFNI CGCG 1 cut(s) 581
BstKTI GATC 6 cut(s) 225, 350, 478, 586, 594, 613
BstMBI GATC 6 cut(s) 222, 347, 475, 583, 591, 610
BstMCI CGRYCG 2 cut(s) 350, 640
BstMWI GCNNNNNNNGC 4 cut(s) 503, 509, 530, 719
BstNSI RCATGY 2 cut(s) 389, 857
BstUI CGCG 1 cut(s) 581
BstV2I GAAGAC 1 cut(s) 810
BstX2I RGATCY 1 cut(s) 583
BstYI RGATCY 1 cut(s) 583
BstZI CGGCCG 1 cut(s) 637
BsuRI GGCC 3 cut(s) 442, 503, 639
BtgI CCRYGG 4 cut(s) 302, 376, 707, 750
BtsCI GGATG 4 cut(s) 18, 132, 277, 865
BtsIMutI CAGTG 1 cut(s) 288
Cac8I GCNNGC 1 cut(s) 855
CseI GACGC 1 cut(s) 704
Csp6I GTAC 4 cut(s) 151, 333, 454, 831
CviAII CATG 8 cut(s) 343, 386, 528, 614, 659, 761, 814, 854
CviQI GTAC 4 cut(s) 151, 333, 454, 831
DdeI CTNAG 3 cut(s) 450, 492, 498
DpnI GATC 6 cut(s) 224, 349, 477, 585, 593, 612
DpnII GATC 6 cut(s) 222, 347, 475, 583, 591, 610
DraIII CACNNNGTG 1 cut(s) 308
EaeI YGGCCR 1 cut(s) 637
EagI CGGCCG 1 cut(s) 637
Eam1104I CTCTTC 1 cut(s) 93
EarI CTCTTC 1 cut(s) 93
EciI GGCGGA 1 cut(s) 396
EclXI CGGCCG 1 cut(s) 637
Eco24I GRGCYC 1 cut(s) 629
Eco32I GATATC 1 cut(s) 322
Eco52I CGGCCG 1 cut(s) 637
Eco57I CTGAAG 1 cut(s) 608
EcoRV GATATC 1 cut(s) 322
EcoT22I ATGCAT 1 cut(s) 664
EcoT38I GRGCYC 1 cut(s) 629
FaeI CATG 8 cut(s) 346, 389, 531, 617, 662, 764, 817, 857
FaqI GGGAC 2 cut(s) 299, 661
FatI CATG 8 cut(s) 342, 385, 527, 613, 658, 760, 813, 853
FbaI TGATCA 1 cut(s) 475
Fnu4HI GCNGC 1 cut(s) 504
FokI GGATG 4 cut(s) 5, 119, 284, 872
FriOI GRGCYC 1 cut(s) 629
Fsp4HI GCNGC 1 cut(s) 504
FspBI CTAG 2 cut(s) 51, 785
GluI GCNGC 1 cut(s) 504
GsaI CCCAGC 1 cut(s) 491
HaeIII GGCC 3 cut(s) 442, 503, 639
HapII CCGG 2 cut(s) 261, 426
HgaI GACGC 1 cut(s) 704
Hin1I GRCGYC 1 cut(s) 696
Hin1II CATG 8 cut(s) 346, 389, 531, 617, 662, 764, 817, 857
HindIII AAGCTT 2 cut(s) 7, 879
HinfI GANTC 4 cut(s) 25, 215, 394, 649
HpaII CCGG 2 cut(s) 261, 426
HphI GGTGA 2 cut(s) 365, 454
Hpy188I TCNGA 2 cut(s) 187, 588
Hpy188III TCNNGA 4 cut(s) 22, 288, 351, 426
Hpy99I CGWCG 2 cut(s) 206, 698
HpyAV CCTTC 1 cut(s) 453
HpyCH4III ACNGT 6 cut(s) 95, 283, 306, 377, 403, 751
HpyCH4V TGCA 7 cut(s) 4, 15, 512, 524, 662, 760, 853
HpyF10VI GCNNNNNNNGC 4 cut(s) 503, 509, 530, 719
HpyF3I CTNAG 3 cut(s) 450, 492, 498
Hsp92I GRCGYC 1 cut(s) 696
Hsp92II CATG 8 cut(s) 346, 389, 531, 617, 662, 764, 817, 857
Kpn2I TCCGGA 1 cut(s) 425
KpnI GGTACC 1 cut(s) 336
Ksp22I TGATCA 1 cut(s) 475
Kzo9I GATC 6 cut(s) 222, 347, 475, 583, 591, 610
LmnI GCTCC 3 cut(s) 624, 800, 806
LpnPI CCDG 9 cut(s) 35, 274, 330, 439, 485, 501, 519, 589, 819
LweI GCATC 6 cut(s) 75, 141, 262, 288, 671, 781
MaeI CTAG 2 cut(s) 51, 785
MaeIII GTNAC 1 cut(s) 95
MalI GATC 6 cut(s) 224, 349, 477, 585, 593, 612
MboI GATC 6 cut(s) 222, 347, 475, 583, 591, 610
MboII GAAGA 4 cut(s) 80, 412, 601, 810
MflI RGATCY 1 cut(s) 583
MhlI GDGCHC 1 cut(s) 629
MluCI AATT 3 cut(s) 161, 241, 873
MlyI GAGTC 3 cut(s) 224, 388, 643
Mph1103I ATGCAT 1 cut(s) 664
MroI TCCGGA 1 cut(s) 425
MseI TTAA 3 cut(s) 33, 327, 876
MslI CAYNNNNRTG 4 cut(s) 341, 375, 618, 858
MspA1I CMGCKG 1 cut(s) 491
MspI CCGG 2 cut(s) 261, 426
MvnI CGCG 1 cut(s) 581
MwoI GCNNNNNNNGC 4 cut(s) 503, 509, 530, 719
NdeII GATC 6 cut(s) 222, 347, 475, 583, 591, 610
NlaIII CATG 8 cut(s) 346, 389, 531, 617, 662, 764, 817, 857
NlaIV GGNNCC 2 cut(s) 334, 626
NmeAIII GCCGAG 1 cut(s) 665
NmuCI GTSAC 1 cut(s) 95
NsiI ATGCAT 1 cut(s) 664
NspI RCATGY 2 cut(s) 389, 857
PaeI GCATGC 1 cut(s) 857
PciI ACATGT 1 cut(s) 385
PcsI WCGNNNNNNNCGW 1 cut(s) 293
PfeI GAWTC 1 cut(s) 25
PflMI CCANNNNNTGG 2 cut(s) 308, 677
PkrI GCNGC 1 cut(s) 505
Ple19I CGATCG 1 cut(s) 350
PleI GAGTC 3 cut(s) 223, 388, 643
PpsI GAGTC 3 cut(s) 223, 388, 643
PscI ACATGT 1 cut(s) 385
PshBI ATTAAT 1 cut(s) 327
PspFI CCCAGC 1 cut(s) 487
PspN4I GGNNCC 2 cut(s) 334, 626
PsuI RGATCY 1 cut(s) 583
PvuI CGATCG 1 cut(s) 350
PvuII CAGCTG 1 cut(s) 491
RsaI GTAC 4 cut(s) 152, 334, 455, 832
RsaNI GTAC 4 cut(s) 151, 333, 454, 831
RseI CAYNNNNRTG 4 cut(s) 341, 375, 618, 858
SaqAI TTAA 3 cut(s) 33, 327, 876
SatI GCNGC 1 cut(s) 504
Sau3AI GATC 6 cut(s) 222, 347, 475, 583, 591, 610
SchI GAGTC 3 cut(s) 224, 388, 643
SduI GDGCHC 1 cut(s) 629
SfaNI GCATC 6 cut(s) 75, 141, 262, 288, 671, 781
SmiMI CAYNNNNRTG 4 cut(s) 341, 375, 618, 858
SmlI CTYRAG 1 cut(s) 251
SmoI CTYRAG 1 cut(s) 251
SpeI ACTAGT 1 cut(s) 50
SphI GCATGC 1 cut(s) 857
Sse9I AATT 3 cut(s) 161, 241, 873
SsiI CCGC 4 cut(s) 381, 504, 581, 857
SspMI CTAG 2 cut(s) 51, 785
TaaI ACNGT 6 cut(s) 95, 283, 306, 377, 403, 751
TaqI TCGA 4 cut(s) 201, 287, 392, 821
TasI AATT 3 cut(s) 161, 241, 873
TauI GCSGC 1 cut(s) 506
TfiI GAWTC 1 cut(s) 25
Tru1I TTAA 3 cut(s) 33, 327, 876
Tru9I TTAA 3 cut(s) 33, 327, 876
TscAI CASTG 1 cut(s) 288
TseFI GTSAC 1 cut(s) 95
Tsp45I GTSAC 1 cut(s) 95
TspDTI ATGAA 1 cut(s) 777
TspGWI ACGGA 1 cut(s) 787
TspRI CASTG 1 cut(s) 288
Van91I CCANNNNNTGG 2 cut(s) 308, 677
VspI ATTAAT 1 cut(s) 327
XapI RAATTY 1 cut(s) 161
XceI RCATGY 2 cut(s) 389, 857
XspI CTAG 2 cut(s) 51, 785
Zsp2I ATGCAT 1 cut(s) 664
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.