Rroxscaffold_2G00081550

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000309
Physical Location & Seq
Forward (+)
4623826 .. 4626685
2860 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00081550.1

Sequence Viewer

Length: 930 bp
ATGGCATCAAAATCTAATATTTTTGTCTTCACTATCACTCTCCTCCTCGCCATTCTTACTCTCCTCTCTTATGCAACTGCTTCTCATCCCCACAACAATAGCTCTTCTCCATTTGAGTTCCTTGAGCATCTCAAGGGTTGTCACAAGGGTGATAAGGTCAAAGGCATCAATGACCTCAAGAAATACCTTGAAAAGTACGGTTACTTGAACTACAGGAACCAGAGTCATTCAAGCGACGATGATTTCGACGACCTTTTGGAGGACGCCGTGAAGACTTACCAGATCAATTACCACCTCAAGGCCACCGGAACATTGGACGGCAAAACAGTGTCAAAGATGATGACGCCTCGTTGTGGCGTGCCGGATATCATCAATGGCACCTCGTCCATGCGATCAGCCAAGAAAAGGCACCACCATGGTTCAATTCATACCACTGCTCACTACTCATTTTTCCAGGGAAATCCAAAATGGCCTTCCTCAAAATACCATCTCACTTACGGTTTTCTCCCCAACACCCCATCCGAAGCCACGGGCGCTGTCGCACGGGCTTTCGCAACATGGGCTGGAAACACTCACTTCACGTTCAGCCAGTCCCAAAGTTACGAGAATGCGGATTTGAAGATTAGTTTTCATAAGGGTGATCATGGAGACCGGAATTCATTTGATGGCCCGGGTGGGGTCCTAGCCCATGCTTTTAGCCCGTCGGATGGAAGATTCCACTACGACGCAGATGAGACTTGGTCTGTGGGTGCTACTCCAGGTGCTATGGACTTGGAGAGTGTGGCTTTGCATGAAATAGGGCACCTTCTGGGACTTGGACATAGCTCAGTGGAGGGAGCTATCATGGCCCCAGTTATTCCCTCGGGAGTGGCTCGAATAAGTTTGACTGGGGACGATGTTCAAGGAATAAGGGCTTTATACAACGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

309

Amino Acids

33.77

Weight (kDa)

6.69

Isoelectric Point (pI)

32.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 56 - 113 1e-10 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 156 - 307 3.6e-47 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 377, 408, 801
AciI CCGC 1 cut(s) 611
AcsI RAATTY 1 cut(s) 655
AcyI GRCGYC 2 cut(s) 264, 344
AfaI GTAC 1 cut(s) 197
AfiI CCNNNNNNNGG 6 cut(s) 259, 298, 353, 405, 676, 707
AgsI TTSAA 6 cut(s) 191, 208, 231, 423, 619, 902
AjnI CCWGG 2 cut(s) 453, 757
AjuI GAANNNNNNNTTGG 2 cut(s) 457, 489
AleI CACNNNNGTG 1 cut(s) 147
AluBI AGCT 3 cut(s) 102, 825, 839
AluI AGCT 3 cut(s) 102, 825, 839
Alw26I GTCTC 2 cut(s) 642, 728
Ama87I CYCGRG 2 cut(s) 670, 862
AoxI GGCC 4 cut(s) 300, 470, 667, 846
ApoI RAATTY 1 cut(s) 655
ArsI GACNNNNNNTTYG 2 cut(s) 227, 259
AspLEI GCGC 1 cut(s) 536
AspS9I GGNCC 3 cut(s) 668, 679, 847
AsuC2I CCSGG 2 cut(s) 671, 672
AsuHPI GGTGA 2 cut(s) 161, 650
AvaI CYCGRG 2 cut(s) 670, 862
AvaII GGWCC 1 cut(s) 679
BaeGI GKGCMC 1 cut(s) 804
BanI GGYRCC 3 cut(s) 377, 408, 801
BbsI GAAGAC 2 cut(s) 19, 278
BccI CCATC 4 cut(s) 495, 526, 659, 701
BceAI ACGGC 2 cut(s) 251, 334
BciT130I CCWGG 2 cut(s) 455, 759
BclI TGATCA 1 cut(s) 640
BcnI CCSGG 2 cut(s) 671, 672
BcoDI GTCTC 2 cut(s) 642, 728
BfaI CTAG 1 cut(s) 683
BfmI CTRYAG 1 cut(s) 211
BfoI RGCGCY 1 cut(s) 537
Bme1390I CCNGG 4 cut(s) 455, 671, 672, 759
Bme18I GGWCC 1 cut(s) 679
BmeT110I CYCGRG 2 cut(s) 670, 862
BmgT120I GGNCC 3 cut(s) 668, 679, 847
BmiI GGNNCC 6 cut(s) 218, 379, 410, 680, 803, 849
BmrFI CCNGG 4 cut(s) 455, 671, 672, 759
BmrI ACTGGG 2 cut(s) 845, 897
BmsI GCATC 3 cut(s) 14, 136, 174
BmuI ACTGGG 2 cut(s) 845, 897
BpiI GAAGAC 2 cut(s) 19, 278
BpmI CTGGAG 1 cut(s) 741
BpuEI CTTGAG 4 cut(s) 116, 143, 161, 281
BpuMI CCSGG 2 cut(s) 671, 672
BsaHI GRCGYC 2 cut(s) 264, 344
BsaI GGTCTC 1 cut(s) 642
BsaJI CCNNGG 5 cut(s) 415, 454, 528, 670, 861
BsaWI WCCGGW 2 cut(s) 305, 651
Bsc4I CCNNNNNNNGG 6 cut(s) 259, 298, 353, 405, 676, 707
Bse1I ACTGG 3 cut(s) 589, 851, 892
BseBI CCWGG 2 cut(s) 455, 759
BseDI CCNNGG 5 cut(s) 415, 454, 528, 670, 861
BseGI GGATG 3 cut(s) 85, 518, 712
BseLI CCNNNNNNNGG 6 cut(s) 259, 298, 353, 405, 676, 707
BseMII CTCAG 1 cut(s) 840
BseNI ACTGG 3 cut(s) 589, 851, 892
BseRI GAGGAG 3 cut(s) 32, 35, 53
BseSI GKGCMC 1 cut(s) 804
BshFI GGCC 4 cut(s) 302, 472, 669, 848
BshNI GGYRCC 3 cut(s) 377, 408, 801
BsiHKCI CYCGRG 2 cut(s) 670, 862
BsiSI CCGG 4 cut(s) 306, 362, 652, 671
BslFI GGGAC 3 cut(s) 577, 825, 905
BslI CCNNNNNNNGG 6 cut(s) 259, 298, 353, 405, 676, 707
BsmAI GTCTC 2 cut(s) 642, 728
BsmFI GGGAC 3 cut(s) 577, 825, 905
BsmI GAATGC 1 cut(s) 613
BsnI GGCC 4 cut(s) 302, 472, 669, 848
Bso31I GGTCTC 1 cut(s) 642
BsoBI CYCGRG 2 cut(s) 670, 862
Bsp1286I GDGCHC 1 cut(s) 804
Bsp143I GATC 3 cut(s) 282, 392, 640
Bsp19I CCATGG 1 cut(s) 415
BspACI CCGC 1 cut(s) 611
BspANI GGCC 4 cut(s) 302, 472, 669, 848
BspCNI CTCAG 1 cut(s) 839
BspLI GGNNCC 6 cut(s) 218, 379, 410, 680, 803, 849
BspQI GCTCTTC 1 cut(s) 109
BspT107I GGYRCC 3 cut(s) 377, 408, 801
BspTNI GGTCTC 1 cut(s) 642
BsrI ACTGG 3 cut(s) 589, 851, 892
BssECI CCNNGG 5 cut(s) 415, 454, 528, 670, 861
BssMI GATC 3 cut(s) 282, 392, 640
BssNI GRCGYC 2 cut(s) 264, 344
BssT1I CCWWGG 1 cut(s) 415
Bst2UI CCWGG 2 cut(s) 455, 759
Bst4CI ACNGT 3 cut(s) 200, 328, 500
Bst6I CTCTTC 1 cut(s) 109
BstACI GRCGYC 2 cut(s) 264, 344
BstC8I GCNNGC 1 cut(s) 359
BstDEI CTNAG 1 cut(s) 826
BstDSI CCRYGG 2 cut(s) 415, 528
BstENI CCTNNNNNAGG 1 cut(s) 257
BstF5I GGATG 3 cut(s) 85, 518, 712
BstH2I RGCGCY 1 cut(s) 537
BstHHI GCGC 1 cut(s) 536
BstKTI GATC 3 cut(s) 285, 395, 643
BstMAI GTCTC 2 cut(s) 642, 728
BstMBI GATC 3 cut(s) 282, 392, 640
BstMWI GCNNNNNNNGC 3 cut(s) 533, 560, 845
BstNI CCWGG 2 cut(s) 455, 759
BstSCI CCNGG 4 cut(s) 453, 669, 670, 757
BstSFI CTRYAG 1 cut(s) 211
BstSLI GKGCMC 1 cut(s) 804
BstV2I GAAGAC 2 cut(s) 19, 278
BsuRI GGCC 4 cut(s) 302, 472, 669, 848
BtgI CCRYGG 2 cut(s) 415, 528
BtsCI GGATG 3 cut(s) 85, 518, 712
BtsI GCAGTG 1 cut(s) 432
BtsIMutI CAGTG 3 cut(s) 333, 432, 834
Cac8I GCNNGC 1 cut(s) 359
CfoI GCGC 1 cut(s) 536
Cfr13I GGNCC 3 cut(s) 668, 679, 847
Cfr9I CCCGGG 1 cut(s) 670
CseI GACGC 3 cut(s) 272, 352, 734
Csp6I GTAC 1 cut(s) 196
CviAII CATG 7 cut(s) 388, 416, 558, 644, 689, 791, 844
CviQI GTAC 1 cut(s) 196
DdeI CTNAG 1 cut(s) 826
DpnI GATC 3 cut(s) 284, 394, 642
DpnII GATC 3 cut(s) 282, 392, 640
Eam1104I CTCTTC 1 cut(s) 109
EarI CTCTTC 1 cut(s) 109
Eco130I CCWWGG 1 cut(s) 415
Eco31I GGTCTC 1 cut(s) 642
Eco32I GATATC 1 cut(s) 367
Eco47I GGWCC 1 cut(s) 679
Eco88I CYCGRG 2 cut(s) 670, 862
EcoNI CCTNNNNNAGG 1 cut(s) 257
EcoO109I RGGNCCY 1 cut(s) 679
EcoRI GAATTC 1 cut(s) 655
EcoRII CCWGG 2 cut(s) 453, 757
EcoRV GATATC 1 cut(s) 367
EcoT14I CCWWGG 1 cut(s) 415
ErhI CCWWGG 1 cut(s) 415
FaeI CATG 7 cut(s) 391, 419, 561, 647, 692, 794, 847
FaqI GGGAC 3 cut(s) 577, 825, 905
FatI CATG 7 cut(s) 387, 415, 557, 643, 688, 790, 843
FbaI TGATCA 1 cut(s) 640
FokI GGATG 3 cut(s) 72, 505, 719
FspBI CTAG 1 cut(s) 683
GlaI GCGC 1 cut(s) 535
GsuI CTGGAG 1 cut(s) 741
HaeII RGCGCY 1 cut(s) 537
HaeIII GGCC 4 cut(s) 302, 472, 669, 848
HapII CCGG 4 cut(s) 306, 362, 652, 671
HgaI GACGC 3 cut(s) 272, 352, 734
HhaI GCGC 1 cut(s) 536
Hin1I GRCGYC 2 cut(s) 264, 344
Hin1II CATG 7 cut(s) 391, 419, 561, 647, 692, 794, 847
Hin6I GCGC 1 cut(s) 534
HinP1I GCGC 1 cut(s) 534
HinfI GANTC 2 cut(s) 223, 714
HpaII CCGG 4 cut(s) 306, 362, 652, 671
HphI GGTGA 2 cut(s) 161, 650
Hpy188I TCNGA 3 cut(s) 523, 706, 929
Hpy188III TCNNGA 2 cut(s) 178, 864
Hpy99I CGWCG 4 cut(s) 239, 251, 706, 728
HpyAV CCTTC 2 cut(s) 483, 815
HpyCH4III ACNGT 3 cut(s) 200, 328, 500
HpyCH4IV ACGT 2 cut(s) 581, 924
HpyCH4V TGCA 2 cut(s) 74, 790
HpyF10VI GCNNNNNNNGC 3 cut(s) 533, 560, 845
HpyF3I CTNAG 1 cut(s) 826
HpySE526I ACGT 2 cut(s) 581, 924
Hsp92I GRCGYC 2 cut(s) 264, 344
Hsp92II CATG 7 cut(s) 391, 419, 561, 647, 692, 794, 847
HspAI GCGC 1 cut(s) 534
Ksp22I TGATCA 1 cut(s) 640
Kzo9I GATC 3 cut(s) 282, 392, 640
LguI GCTCTTC 1 cut(s) 109
LmnI GCTCC 1 cut(s) 836
LweI GCATC 3 cut(s) 14, 136, 174
MaeI CTAG 1 cut(s) 683
MaeII ACGT 2 cut(s) 581, 924
MaeIII GTNAC 3 cut(s) 140, 200, 599
MalI GATC 3 cut(s) 284, 394, 642
MboI GATC 3 cut(s) 282, 392, 640
MboII GAAGA 5 cut(s) 19, 96, 283, 631, 723
MhlI GDGCHC 1 cut(s) 804
MluCI AATT 3 cut(s) 286, 423, 655
MlyI GAGTC 1 cut(s) 232
MmeI TCCRAC 1 cut(s) 684
MslI CAYNNNNRTG 3 cut(s) 147, 414, 636
MspI CCGG 4 cut(s) 306, 362, 652, 671
MspR9I CCNGG 4 cut(s) 455, 671, 672, 759
Mva1269I GAATGC 1 cut(s) 613
MvaI CCWGG 2 cut(s) 455, 759
MwoI GCNNNNNNNGC 3 cut(s) 533, 560, 845
NciI CCSGG 2 cut(s) 671, 672
NcoI CCATGG 1 cut(s) 415
NdeII GATC 3 cut(s) 282, 392, 640
NlaIII CATG 7 cut(s) 391, 419, 561, 647, 692, 794, 847
NlaIV GGNNCC 6 cut(s) 218, 379, 410, 680, 803, 849
NmuCI GTSAC 1 cut(s) 140
OliI CACNNNNGTG 1 cut(s) 147
PciSI GCTCTTC 1 cut(s) 109
PcsI WCGNNNNNNNCGW 1 cut(s) 243
PctI GAATGC 1 cut(s) 613
PfeI GAWTC 1 cut(s) 714
PflFI GACNNNGTC 1 cut(s) 739
PleI GAGTC 1 cut(s) 231
PpsI GAGTC 1 cut(s) 231
PpuMI RGGWCCY 1 cut(s) 679
Psp5II RGGWCCY 1 cut(s) 679
Psp6I CCWGG 2 cut(s) 453, 757
PspGI CCWGG 2 cut(s) 453, 757
PspN4I GGNNCC 6 cut(s) 218, 379, 410, 680, 803, 849
PspPI GGNCC 3 cut(s) 668, 679, 847
PspPPI RGGWCCY 1 cut(s) 679
PsyI GACNNNGTC 1 cut(s) 739
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
RseI CAYNNNNRTG 3 cut(s) 147, 414, 636
SapI GCTCTTC 1 cut(s) 109
Sau3AI GATC 3 cut(s) 282, 392, 640
Sau96I GGNCC 3 cut(s) 668, 679, 847
SchI GAGTC 1 cut(s) 232
ScrFI CCNGG 4 cut(s) 455, 671, 672, 759
SduI GDGCHC 1 cut(s) 804
SfaNI GCATC 3 cut(s) 14, 136, 174
SfcI CTRYAG 1 cut(s) 211
SinI GGWCC 1 cut(s) 679
SmaI CCCGGG 1 cut(s) 672
SmiMI CAYNNNNRTG 3 cut(s) 147, 414, 636
SmlI CTYRAG 4 cut(s) 122, 131, 176, 296
SmoI CTYRAG 4 cut(s) 122, 131, 176, 296
Sse9I AATT 3 cut(s) 286, 423, 655
SsiI CCGC 1 cut(s) 611
SspI AATATT 1 cut(s) 19
SspMI CTAG 1 cut(s) 683
StyD4I CCNGG 4 cut(s) 453, 669, 670, 757
StyI CCWWGG 1 cut(s) 415
TaaI ACNGT 3 cut(s) 200, 328, 500
TaiI ACGT 2 cut(s) 584, 927
TaqI TCGA 2 cut(s) 246, 874
TasI AATT 3 cut(s) 286, 423, 655
TfiI GAWTC 1 cut(s) 714
TscAI CASTG 3 cut(s) 333, 439, 834
TseFI GTSAC 1 cut(s) 140
Tsp45I GTSAC 1 cut(s) 140
TspDTI ATGAA 4 cut(s) 416, 620, 648, 807
TspMI CCCGGG 1 cut(s) 670
TspRI CASTG 3 cut(s) 333, 439, 834
Tth111I GACNNNGTC 1 cut(s) 739
VpaK11BI GGWCC 1 cut(s) 679
XagI CCTNNNNNAGG 1 cut(s) 257
XapI RAATTY 1 cut(s) 655
XcmI CCANNNNNNNNNTGG 1 cut(s) 310
XmaI CCCGGG 1 cut(s) 670
XspI CTAG 1 cut(s) 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.