Rorug02G0546800

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000309
Physical Location & Seq
Forward (+)
67528654 .. 67531501
2848 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0546800.1

Sequence Viewer

Length: 849 bp
ATGGCGACCGCCTTGAGCTGCGGCCAGACCATCATTCTCGCTCCTCCTTCTCGCATCACCTCTCGGGATGTAAATCTTAGACAACTCACCAAGCCAAACAGGGTTGCATGCAACTTCACTCCAAAGTCACTGCCTATATTTTCAACCAGCAGACCACCTAATCAACTTCTGCCTGTACTGCCCAGAGGATTGCCAAGCTTGGACCTTTCTGCTGCATCATCCACAGTGCTAAGGGGGAATGAAGCTGGCCGGTCACAGAAAACATCCATCCTACTCAGACAACGCAAATCTTCAACATTGGTACAAGCAAAGAAGGATAATAGTTCAATCACTATACCTTTCCCGAAAATGACTGAGAAGCCAGAGTGGTGGTGGAGAACTTTAGCATGTCTCCCTTATTTAGCATCTTTGCAGATGTCCGAGGTTGGCTTTTATATTCAACCCTTTGCAGAACACTATCAACTTTTTGGAGATTTGATTTATTTTGTCCCCGGAGCAATAAAGAGGTTACCACAGTGGTTCACAATGACATATGTCATGGTGGCATATTTTGGAGTGGTGAAGAAGAAACAGTGGCCTCACTTCTTTAGGTACCATGTTATTATGGCCATGTTATTAGAAACGATGCTGCAAGTGGTAGCGCAGTCGAGCAATTTCTTTCCGCTTATACACTTCAATGGTACATTTGGCATAGAGTATTGGGCGGCTGTGGCATTCCTGTACATTTTGATCTTGTTGGAGTGCATAAGGTGTGCTCTTGCTGGCAAGTATGTTAAGCTCCCGTTTTTCAGTACTCCTGCTCTAGCTCATACTCTTTTCACCGTAGAAGGCAGGTACAGACCTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

282

Amino Acids

32.1

Weight (kDa)

9.92

Isoelectric Point (pI)

53.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIC20 PF16166 93 - 266 1.5e-68 Chloroplast import apparatus Tic20-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 822
Acc65I GGTACC 1 cut(s) 591
AccB1I GGYRCC 1 cut(s) 591
AciI CCGC 4 cut(s) 9, 21, 662, 704
AcoI YGGCCR 3 cut(s) 22, 247, 606
AfaI GTAC 7 cut(s) 177, 303, 593, 682, 722, 793, 836
AgsI TTSAA 5 cut(s) 144, 294, 327, 440, 676
AluBI AGCT 5 cut(s) 18, 198, 245, 778, 806
AluI AGCT 5 cut(s) 18, 198, 245, 778, 806
Alw21I GWGCWC 1 cut(s) 757
Alw26I GTCTC 1 cut(s) 395
Ama87I CYCGRG 1 cut(s) 63
AoxI GGCC 4 cut(s) 22, 247, 575, 606
ApeKI GCWGC 3 cut(s) 18, 212, 628
Asp718I GGTACC 1 cut(s) 591
AspLEI GCGC 1 cut(s) 643
AspS9I GGNCC 1 cut(s) 202
AsuC2I CCSGG 1 cut(s) 492
AsuHPI GGTGA 4 cut(s) 49, 79, 571, 811
AvaI CYCGRG 1 cut(s) 63
AvaII GGWCC 1 cut(s) 202
BalI TGGCCA 1 cut(s) 608
BanI GGYRCC 1 cut(s) 591
Bbv12I GWGCWC 1 cut(s) 757
BbvI GCAGC 3 cut(s) 5, 199, 615
BccI CCATC 2 cut(s) 38, 275
BcnI CCSGG 1 cut(s) 492
BcoDI GTCTC 1 cut(s) 395
BfaI CTAG 1 cut(s) 803
BfuAI ACCTGC 1 cut(s) 822
BisI GCNGC 5 cut(s) 19, 22, 213, 629, 705
BlsI GCNGC 5 cut(s) 20, 23, 214, 630, 706
BmcAI AGTACT 1 cut(s) 793
Bme1390I CCNGG 1 cut(s) 492
Bme18I GGWCC 1 cut(s) 202
BmeT110I CYCGRG 1 cut(s) 63
BmgT120I GGNCC 1 cut(s) 202
BmiI GGNNCC 1 cut(s) 593
BmrFI CCNGG 1 cut(s) 492
BmsI GCATC 4 cut(s) 63, 224, 413, 615
BoxI GACNNNNGTC 1 cut(s) 533
Bpu10I CCTNAGC 1 cut(s) 230
BpuEI CTTGAG 1 cut(s) 34
BpuMI CCSGG 1 cut(s) 492
BsaBI GATNNNNATC 1 cut(s) 72
BsaJI CCNNGG 2 cut(s) 420, 490
Bse118I RCCGGY 1 cut(s) 249
Bse8I GATNNNNATC 1 cut(s) 72
BseDI CCNNGG 2 cut(s) 420, 490
BseGI GGATG 4 cut(s) 73, 218, 263, 267
BseJI GATNNNNATC 1 cut(s) 72
BseMII CTCAG 2 cut(s) 289, 345
BseRI GAGGAG 1 cut(s) 33
BseXI GCAGC 3 cut(s) 5, 199, 615
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 4 cut(s) 24, 249, 577, 608
BshNI GGYRCC 1 cut(s) 591
BsiEI CGRYCG 1 cut(s) 9
BsiHKAI GWGCWC 1 cut(s) 757
BsiHKCI CYCGRG 1 cut(s) 63
BsiSI CCGG 2 cut(s) 250, 492
BslFI GGGAC 1 cut(s) 473
BsmAI GTCTC 1 cut(s) 395
BsmFI GGGAC 1 cut(s) 473
BsmI GAATGC 1 cut(s) 713
BsnI GGCC 4 cut(s) 24, 249, 577, 608
BsoBI CYCGRG 1 cut(s) 63
Bsp1286I GDGCHC 1 cut(s) 757
Bsp1407I TGTACA 1 cut(s) 720
Bsp143I GATC 1 cut(s) 729
BspACI CCGC 4 cut(s) 9, 21, 662, 704
BspANI GGCC 4 cut(s) 24, 249, 577, 608
BspCNI CTCAG 2 cut(s) 288, 346
BspLI GGNNCC 1 cut(s) 593
BspMI ACCTGC 1 cut(s) 822
BspT107I GGYRCC 1 cut(s) 591
BsrFI RCCGGY 1 cut(s) 249
BsrGI TGTACA 1 cut(s) 720
BssAI RCCGGY 1 cut(s) 249
BssECI CCNNGG 2 cut(s) 420, 490
BssMI GATC 1 cut(s) 729
Bst4CI ACNGT 4 cut(s) 226, 516, 573, 823
BstAUI TGTACA 1 cut(s) 720
BstC8I GCNNGC 3 cut(s) 109, 247, 763
BstDEI CTNAG 4 cut(s) 77, 230, 275, 354
BstEII GGTNACC 1 cut(s) 507
BstF5I GGATG 4 cut(s) 73, 218, 263, 267
BstHHI GCGC 1 cut(s) 643
BstKTI GATC 1 cut(s) 732
BstMAI GTCTC 1 cut(s) 395
BstMBI GATC 1 cut(s) 729
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 2 cut(s) 178, 710
BstNSI RCATGY 2 cut(s) 111, 390
BstPAI GACNNNNGTC 1 cut(s) 533
BstPI GGTNACC 1 cut(s) 507
BstSCI CCNGG 1 cut(s) 490
BstV1I GCAGC 3 cut(s) 5, 199, 615
BstXI CCANNNNNNTGG 1 cut(s) 369
BsuRI GGCC 4 cut(s) 24, 249, 577, 608
BtsCI GGATG 4 cut(s) 73, 218, 263, 267
BtsI GCAGTG 1 cut(s) 128
BtsIMutI CAGTG 4 cut(s) 128, 231, 521, 578
BveI ACCTGC 1 cut(s) 822
Cac8I GCNNGC 3 cut(s) 109, 247, 763
CfoI GCGC 1 cut(s) 643
Cfr10I RCCGGY 1 cut(s) 249
Cfr13I GGNCC 1 cut(s) 202
Csp6I GTAC 7 cut(s) 176, 302, 592, 681, 721, 792, 835
CviAII CATG 5 cut(s) 108, 387, 538, 596, 610
CviQI GTAC 7 cut(s) 176, 302, 592, 681, 721, 792, 835
DdeI CTNAG 4 cut(s) 77, 230, 275, 354
DpnI GATC 1 cut(s) 731
DpnII GATC 1 cut(s) 729
EaeI YGGCCR 3 cut(s) 22, 247, 606
Eco47I GGWCC 1 cut(s) 202
Eco88I CYCGRG 1 cut(s) 63
Eco91I GGTNACC 1 cut(s) 507
EcoO65I GGTNACC 1 cut(s) 507
FaeI CATG 5 cut(s) 111, 390, 541, 599, 613
FaqI GGGAC 1 cut(s) 473
FatI CATG 5 cut(s) 107, 386, 537, 595, 609
FauNDI CATATG 1 cut(s) 532
Fnu4HI GCNGC 5 cut(s) 19, 22, 213, 629, 705
FokI GGATG 4 cut(s) 80, 205, 250, 254
Fsp4HI GCNGC 5 cut(s) 19, 22, 213, 629, 705
FspBI CTAG 1 cut(s) 803
GlaI GCGC 1 cut(s) 642
GluI GCNGC 5 cut(s) 19, 22, 213, 629, 705
HaeIII GGCC 4 cut(s) 24, 249, 577, 608
HapII CCGG 2 cut(s) 250, 492
HhaI GCGC 1 cut(s) 643
Hin1II CATG 5 cut(s) 111, 390, 541, 599, 613
Hin6I GCGC 1 cut(s) 641
HinP1I GCGC 1 cut(s) 641
HindIII AAGCTT 1 cut(s) 196
HpaII CCGG 2 cut(s) 250, 492
HphI GGTGA 4 cut(s) 49, 79, 571, 811
Hpy166II GTNNAC 1 cut(s) 522
Hpy188I TCNGA 2 cut(s) 278, 421
Hpy188III TCNNGA 2 cut(s) 65, 343
Hpy8I GTNNAC 1 cut(s) 522
HpyAV CCTTC 3 cut(s) 57, 307, 821
HpyCH4III ACNGT 4 cut(s) 226, 516, 573, 823
HpyCH4V TGCA 7 cut(s) 107, 111, 215, 412, 449, 631, 744
HpyF10VI GCNNNNNNNGC 2 cut(s) 178, 710
HpyF3I CTNAG 4 cut(s) 77, 230, 275, 354
Hsp92II CATG 5 cut(s) 111, 390, 541, 599, 613
HspAI GCGC 1 cut(s) 641
KpnI GGTACC 1 cut(s) 595
Kzo9I GATC 1 cut(s) 729
LmnI GCTCC 3 cut(s) 46, 494, 783
Lsp1109I GCAGC 3 cut(s) 5, 199, 615
LweI GCATC 4 cut(s) 63, 224, 413, 615
MaeI CTAG 1 cut(s) 803
MaeIII GTNAC 3 cut(s) 126, 252, 507
MalI GATC 1 cut(s) 731
MboI GATC 1 cut(s) 729
MboII GAAGA 3 cut(s) 282, 574, 577
MhlI GDGCHC 1 cut(s) 757
MlsI TGGCCA 1 cut(s) 608
MluCI AATT 1 cut(s) 652
MluNI TGGCCA 1 cut(s) 608
MmeI TCCRAC 1 cut(s) 717
MnlI CCTC 6 cut(s) 54, 70, 179, 415, 498, 588
Mox20I TGGCCA 1 cut(s) 608
MscI TGGCCA 1 cut(s) 608
MseI TTAA 2 cut(s) 774, 847
MslI CAYNNNNRTG 1 cut(s) 675
Msp20I TGGCCA 1 cut(s) 608
MspI CCGG 2 cut(s) 250, 492
MspR9I CCNGG 1 cut(s) 492
Mva1269I GAATGC 1 cut(s) 713
MwoI GCNNNNNNNGC 2 cut(s) 178, 710
NciI CCSGG 1 cut(s) 492
NdeI CATATG 1 cut(s) 532
NdeII GATC 1 cut(s) 729
NlaIII CATG 5 cut(s) 111, 390, 541, 599, 613
NlaIV GGNNCC 1 cut(s) 593
NmuCI GTSAC 2 cut(s) 126, 252
NspI RCATGY 2 cut(s) 111, 390
PaeI GCATGC 1 cut(s) 111
PctI GAATGC 1 cut(s) 713
PkrI GCNGC 5 cut(s) 20, 23, 214, 630, 706
PshAI GACNNNNGTC 1 cut(s) 533
PspEI GGTNACC 1 cut(s) 507
PspN4I GGNNCC 1 cut(s) 593
PspPI GGNCC 1 cut(s) 202
RsaI GTAC 7 cut(s) 177, 303, 593, 682, 722, 793, 836
RsaNI GTAC 7 cut(s) 176, 302, 592, 681, 721, 792, 835
RseI CAYNNNNRTG 1 cut(s) 675
SaqAI TTAA 2 cut(s) 774, 847
SatI GCNGC 5 cut(s) 19, 22, 213, 629, 705
Sau3AI GATC 1 cut(s) 729
Sau96I GGNCC 1 cut(s) 202
ScaI AGTACT 1 cut(s) 793
ScrFI CCNGG 1 cut(s) 492
SduI GDGCHC 1 cut(s) 757
SfaNI GCATC 4 cut(s) 63, 224, 413, 615
SinI GGWCC 1 cut(s) 202
SmiMI CAYNNNNRTG 1 cut(s) 675
SmlI CTYRAG 1 cut(s) 13
SmoI CTYRAG 1 cut(s) 13
SphI GCATGC 1 cut(s) 111
Sse9I AATT 1 cut(s) 652
SsiI CCGC 4 cut(s) 9, 21, 662, 704
SspMI CTAG 1 cut(s) 803
StyD4I CCNGG 1 cut(s) 490
TaaI ACNGT 4 cut(s) 226, 516, 573, 823
TaqI TCGA 1 cut(s) 647
TasI AATT 1 cut(s) 652
TatI WGTACW 3 cut(s) 175, 720, 791
TauI GCSGC 2 cut(s) 24, 707
Tru1I TTAA 2 cut(s) 774, 847
Tru9I TTAA 2 cut(s) 774, 847
TscAI CASTG 4 cut(s) 135, 231, 521, 578
TseFI GTSAC 2 cut(s) 126, 252
TseI GCWGC 3 cut(s) 18, 212, 628
Tsp45I GTSAC 2 cut(s) 126, 252
TspDTI ATGAA 1 cut(s) 255
TspRI CASTG 4 cut(s) 135, 231, 521, 578
VpaK11BI GGWCC 1 cut(s) 202
XceI RCATGY 2 cut(s) 111, 390
XcmI CCANNNNNNNNNTGG 1 cut(s) 369
XspI CTAG 1 cut(s) 803
ZrmI AGTACT 1 cut(s) 793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.