Rw2G051260

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000309
Physical Location & Seq
Reverse (-)
78984643 .. 78985830
1188 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G051260.1

Sequence Viewer

Length: 927 bp
ATGGCATCCAAATCTAATCTTTCTCTCTTCACTTTCACTCTCCTCCTCGCCCTTCTTACTCTCCTCTCTTATGCAACTGCTTCTCATCGTCACAACAAGAGCTCTTCCCCATTTGAGTTCCTCGAGCATCTCAAGGGTTGTCACAAGGGAGATAAGGTCAAAGGCATCAATGACCTCAAGAAATACCTTGAAAAGTACGGTTACTTGAACTACAAGAACCACAGTCATTTGGAGGATGATGATTTCGATGACCTTTTGGAGGAAGCCATCAAGACTTACCAGATCAATTACCACCTTAAGGCCACTGGAACATTGGATGGCAAAACAGTGTCGAAGATGATGACGCCTCGTTGTGGCCTGCCGGATATCATCAATGGCACCTCGTCCATGCGATCAGCCAAGAAAAGGCACCACCATGGTTCAATTCATACCACTGCTCACTACTCATTTCCCAATGGAAATCCAAAATGGCCTCCCTCTAAATACCATCTCACTTACGGTTTTCTCCCCGGCACCCCATCCGAAGCCACGGGTGCTGTCGCACGGGCTTTCGCAACATGGGCGGGGAACACTCACTTCACGTTCAGCCAGGCCCAAAGTTACGAGAATGCGGATTTGAAGATTAGTTTTCAGAGGGGTGATCATGGAGATGGGTATCCGTTTAAGGGCTCCGGCGGAGAACTGGCCCATGCTTTTTACCCGTCAGACGGAAGATTCCACTACGACGCAGATGAGACTTGGTCTGTGGGTGCTAAACCAGGTGCTTATGACTTGGAGATGGTGGCTTTGCATGAAATAGGGCACCTTCTTGGACTAGGACATAGCTCAGTGGAGGGAGCTATCATGGAACCGCGTCTTCCCTCGGGAGTGACTCGAGGTTTGCATGGGGACGATGTTCAAGGTATCAAGGCTTTGTATAACGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

308

Amino Acids

34.0

Weight (kDa)

7.08

Isoelectric Point (pI)

26.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 56 - 113 2.5e-10 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 157 - 306 5.4e-44 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 4 cut(s) 377, 408, 512, 801
AccII CGCG 1 cut(s) 853
AciI CCGC 4 cut(s) 563, 611, 675, 851
AcyI GRCGYC 1 cut(s) 344
AfaI GTAC 1 cut(s) 197
AfiI CCNNNNNNNGG 6 cut(s) 259, 298, 353, 405, 665, 707
AflII CTTAAG 1 cut(s) 296
AgsI TTSAA 5 cut(s) 191, 208, 423, 619, 899
AjnI CCWGG 2 cut(s) 588, 757
AluBI AGCT 3 cut(s) 102, 825, 839
AluI AGCT 3 cut(s) 102, 825, 839
Alw21I GWGCWC 1 cut(s) 104
Alw26I GTCTC 1 cut(s) 728
Ama87I CYCGRG 3 cut(s) 122, 862, 873
AoxI GGCC 5 cut(s) 300, 355, 470, 591, 684
ArsI GACNNNNNNTTYG 2 cut(s) 862, 894
AspS9I GGNCC 2 cut(s) 592, 685
AsuC2I CCSGG 1 cut(s) 510
AsuHPI GGTGA 1 cut(s) 650
AvaI CYCGRG 3 cut(s) 122, 862, 873
BaeGI GKGCMC 1 cut(s) 804
BanI GGYRCC 4 cut(s) 377, 408, 512, 801
BanII GRGCYC 2 cut(s) 104, 671
BbsI GAAGAC 1 cut(s) 848
Bbv12I GWGCWC 1 cut(s) 104
BccI CCATC 6 cut(s) 275, 311, 495, 526, 644, 772
BciT130I CCWGG 2 cut(s) 590, 759
BciVI GTATCC 1 cut(s) 666
BclI TGATCA 1 cut(s) 640
BcnI CCSGG 1 cut(s) 510
BcoDI GTCTC 1 cut(s) 728
BfaI CTAG 1 cut(s) 815
BfrI CTTAAG 1 cut(s) 296
BfuI GTATCC 1 cut(s) 666
Bme1390I CCNGG 3 cut(s) 510, 590, 759
BmeT110I CYCGRG 3 cut(s) 122, 862, 873
BmgT120I GGNCC 2 cut(s) 592, 685
BmiI GGNNCC 6 cut(s) 379, 410, 514, 670, 803, 849
BmrFI CCNGG 3 cut(s) 510, 590, 759
BmsI GCATC 3 cut(s) 14, 136, 174
BpiI GAAGAC 1 cut(s) 848
BpuEI CTTGAG 2 cut(s) 116, 161
BpuMI CCSGG 1 cut(s) 510
BsaBI GATNNNNATC 1 cut(s) 654
BsaHI GRCGYC 1 cut(s) 344
BsaJI CCNNGG 4 cut(s) 415, 508, 528, 861
BsaXI ACNNNNNCTCC 2 cut(s) 141, 171
Bsc4I CCNNNNNNNGG 6 cut(s) 259, 298, 353, 405, 665, 707
Bse1I ACTGG 2 cut(s) 310, 687
Bse8I GATNNNNATC 1 cut(s) 654
BseBI CCWGG 2 cut(s) 590, 759
BseDI CCNNGG 4 cut(s) 415, 508, 528, 861
BseGI GGATG 4 cut(s) 5, 241, 322, 518
BseJI GATNNNNATC 1 cut(s) 654
BseLI CCNNNNNNNGG 6 cut(s) 259, 298, 353, 405, 665, 707
BseMII CTCAG 1 cut(s) 840
BseNI ACTGG 2 cut(s) 310, 687
BseRI GAGGAG 3 cut(s) 32, 35, 53
BseSI GKGCMC 1 cut(s) 804
Bsh1236I CGCG 1 cut(s) 853
BshFI GGCC 5 cut(s) 302, 357, 472, 593, 686
BshNI GGYRCC 4 cut(s) 377, 408, 512, 801
BsiHKAI GWGCWC 1 cut(s) 104
BsiHKCI CYCGRG 3 cut(s) 122, 862, 873
BsiSI CCGG 3 cut(s) 362, 510, 672
BslFI GGGAC 1 cut(s) 902
BslI CCNNNNNNNGG 6 cut(s) 259, 298, 353, 405, 665, 707
BsmAI GTCTC 1 cut(s) 728
BsmFI GGGAC 1 cut(s) 902
BsmI GAATGC 1 cut(s) 613
BsnI GGCC 5 cut(s) 302, 357, 472, 593, 686
BsoBI CYCGRG 3 cut(s) 122, 862, 873
Bsp1286I GDGCHC 3 cut(s) 104, 671, 804
Bsp143I GATC 3 cut(s) 282, 392, 640
Bsp19I CCATGG 1 cut(s) 415
BspACI CCGC 4 cut(s) 563, 611, 675, 851
BspANI GGCC 5 cut(s) 302, 357, 472, 593, 686
BspCNI CTCAG 1 cut(s) 839
BspFNI CGCG 1 cut(s) 853
BspLI GGNNCC 6 cut(s) 379, 410, 514, 670, 803, 849
BspQI GCTCTTC 1 cut(s) 109
BspT107I GGYRCC 4 cut(s) 377, 408, 512, 801
BspTI CTTAAG 1 cut(s) 296
BsrI ACTGG 2 cut(s) 310, 687
BssECI CCNNGG 4 cut(s) 415, 508, 528, 861
BssMI GATC 3 cut(s) 282, 392, 640
BssNI GRCGYC 1 cut(s) 344
BssT1I CCWWGG 1 cut(s) 415
Bst2UI CCWGG 2 cut(s) 590, 759
Bst4CI ACNGT 4 cut(s) 200, 224, 328, 500
Bst6I CTCTTC 2 cut(s) 32, 109
BstACI GRCGYC 1 cut(s) 344
BstAFI CTTAAG 1 cut(s) 296
BstC8I GCNNGC 1 cut(s) 359
BstDEI CTNAG 1 cut(s) 826
BstDSI CCRYGG 2 cut(s) 415, 528
BstENI CCTNNNNNAGG 1 cut(s) 257
BstF5I GGATG 4 cut(s) 5, 241, 322, 518
BstFNI CGCG 1 cut(s) 853
BstKTI GATC 3 cut(s) 285, 395, 643
BstMAI GTCTC 1 cut(s) 728
BstMBI GATC 3 cut(s) 282, 392, 640
BstMWI GCNNNNNNNGC 2 cut(s) 533, 560
BstNI CCWGG 2 cut(s) 590, 759
BstSCI CCNGG 3 cut(s) 508, 588, 757
BstSLI GKGCMC 1 cut(s) 804
BstUI CGCG 1 cut(s) 853
BstV2I GAAGAC 1 cut(s) 848
BsuI GTATCC 1 cut(s) 666
BsuRI GGCC 5 cut(s) 302, 357, 472, 593, 686
BtgI CCRYGG 2 cut(s) 415, 528
BtsCI GGATG 4 cut(s) 5, 241, 322, 518
BtsI GCAGTG 1 cut(s) 432
BtsIMutI CAGTG 4 cut(s) 303, 333, 432, 834
Cac8I GCNNGC 1 cut(s) 359
Cfr13I GGNCC 2 cut(s) 592, 685
CseI GACGC 3 cut(s) 352, 734, 842
CsiI ACCWGGT 1 cut(s) 757
Csp6I GTAC 1 cut(s) 196
CviAII CATG 8 cut(s) 388, 416, 558, 644, 689, 791, 844, 884
CviQI GTAC 1 cut(s) 196
DdeI CTNAG 1 cut(s) 826
DpnI GATC 3 cut(s) 284, 394, 642
DpnII GATC 3 cut(s) 282, 392, 640
Eam1104I CTCTTC 2 cut(s) 32, 109
EarI CTCTTC 2 cut(s) 32, 109
EciI GGCGGA 1 cut(s) 690
Ecl136II GAGCTC 1 cut(s) 102
Eco130I CCWWGG 1 cut(s) 415
Eco24I GRGCYC 2 cut(s) 104, 671
Eco32I GATATC 1 cut(s) 367
Eco53kI GAGCTC 1 cut(s) 102
Eco88I CYCGRG 3 cut(s) 122, 862, 873
EcoICRI GAGCTC 1 cut(s) 102
EcoNI CCTNNNNNAGG 1 cut(s) 257
EcoRII CCWGG 2 cut(s) 588, 757
EcoRV GATATC 1 cut(s) 367
EcoT14I CCWWGG 1 cut(s) 415
EcoT38I GRGCYC 2 cut(s) 104, 671
ErhI CCWWGG 1 cut(s) 415
FaeI CATG 8 cut(s) 391, 419, 561, 647, 692, 794, 847, 887
FaqI GGGAC 1 cut(s) 902
FatI CATG 8 cut(s) 387, 415, 557, 643, 688, 790, 843, 883
FauI CCCGC 1 cut(s) 556
FbaI TGATCA 1 cut(s) 640
FokI GGATG 3 cut(s) 248, 329, 505
FriOI GRGCYC 2 cut(s) 104, 671
FspBI CTAG 1 cut(s) 815
HaeIII GGCC 5 cut(s) 302, 357, 472, 593, 686
HapII CCGG 3 cut(s) 362, 510, 672
HgaI GACGC 3 cut(s) 352, 734, 842
Hin1I GRCGYC 1 cut(s) 344
Hin1II CATG 8 cut(s) 391, 419, 561, 647, 692, 794, 847, 887
HinfI GANTC 2 cut(s) 714, 871
HpaII CCGG 3 cut(s) 362, 510, 672
HphI GGTGA 1 cut(s) 650
Hpy188I TCNGA 3 cut(s) 523, 633, 706
Hpy188III TCNNGA 3 cut(s) 178, 271, 864
Hpy99I CGWCG 1 cut(s) 728
HpyAV CCTTC 2 cut(s) 62, 815
HpyCH4III ACNGT 4 cut(s) 200, 224, 328, 500
HpyCH4IV ACGT 2 cut(s) 581, 921
HpyCH4V TGCA 3 cut(s) 74, 790, 883
HpyF10VI GCNNNNNNNGC 2 cut(s) 533, 560
HpyF3I CTNAG 1 cut(s) 826
HpySE526I ACGT 2 cut(s) 581, 921
Hsp92I GRCGYC 1 cut(s) 344
Hsp92II CATG 8 cut(s) 391, 419, 561, 647, 692, 794, 847, 887
Ksp22I TGATCA 1 cut(s) 640
Kzo9I GATC 3 cut(s) 282, 392, 640
LguI GCTCTTC 1 cut(s) 109
LmnI GCTCC 2 cut(s) 674, 836
LweI GCATC 3 cut(s) 14, 136, 174
MabI ACCWGGT 1 cut(s) 757
MaeI CTAG 1 cut(s) 815
MaeII ACGT 2 cut(s) 581, 921
MaeIII GTNAC 5 cut(s) 89, 140, 200, 599, 868
MalI GATC 3 cut(s) 284, 394, 642
MboI GATC 3 cut(s) 282, 392, 640
MboII GAAGA 6 cut(s) 19, 96, 346, 631, 723, 848
MhlI GDGCHC 3 cut(s) 104, 671, 804
MluCI AATT 2 cut(s) 286, 423
MlyI GAGTC 1 cut(s) 865
MseI TTAA 2 cut(s) 297, 663
MslI CAYNNNNRTG 2 cut(s) 414, 648
MspCI CTTAAG 1 cut(s) 296
MspI CCGG 3 cut(s) 362, 510, 672
MspR9I CCNGG 3 cut(s) 510, 590, 759
Mva1269I GAATGC 1 cut(s) 613
MvaI CCWGG 2 cut(s) 590, 759
MvnI CGCG 1 cut(s) 853
MwoI GCNNNNNNNGC 2 cut(s) 533, 560
NciI CCSGG 1 cut(s) 510
NcoI CCATGG 1 cut(s) 415
NdeII GATC 3 cut(s) 282, 392, 640
NlaIII CATG 8 cut(s) 391, 419, 561, 647, 692, 794, 847, 887
NlaIV GGNNCC 6 cut(s) 379, 410, 514, 670, 803, 849
NmuCI GTSAC 3 cut(s) 89, 140, 868
PaeR7I CTCGAG 2 cut(s) 122, 873
PciSI GCTCTTC 1 cut(s) 109
PctI GAATGC 1 cut(s) 613
PfeI GAWTC 1 cut(s) 714
PflFI GACNNNGTC 1 cut(s) 739
PleI GAGTC 1 cut(s) 865
PpsI GAGTC 1 cut(s) 865
Psp124BI GAGCTC 1 cut(s) 104
Psp6I CCWGG 2 cut(s) 588, 757
PspGI CCWGG 2 cut(s) 588, 757
PspN4I GGNNCC 6 cut(s) 379, 410, 514, 670, 803, 849
PspPI GGNCC 2 cut(s) 592, 685
PspXI VCTCGAGB 2 cut(s) 122, 873
PsyI GACNNNGTC 1 cut(s) 739
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
RseI CAYNNNNRTG 2 cut(s) 414, 648
SacI GAGCTC 1 cut(s) 104
SapI GCTCTTC 1 cut(s) 109
SaqAI TTAA 2 cut(s) 297, 663
Sau3AI GATC 3 cut(s) 282, 392, 640
Sau96I GGNCC 2 cut(s) 592, 685
SchI GAGTC 1 cut(s) 865
ScrFI CCNGG 3 cut(s) 510, 590, 759
SduI GDGCHC 3 cut(s) 104, 671, 804
SexAI ACCWGGT 1 cut(s) 757
SfaNI GCATC 3 cut(s) 14, 136, 174
Sfr274I CTCGAG 2 cut(s) 122, 873
SlaI CTCGAG 2 cut(s) 122, 873
SmiMI CAYNNNNRTG 2 cut(s) 414, 648
SmlI CTYRAG 5 cut(s) 122, 131, 176, 296, 873
SmoI CTYRAG 5 cut(s) 122, 131, 176, 296, 873
Sse9I AATT 2 cut(s) 286, 423
SsiI CCGC 4 cut(s) 563, 611, 675, 851
SspMI CTAG 1 cut(s) 815
SstI GAGCTC 1 cut(s) 104
StyD4I CCNGG 3 cut(s) 508, 588, 757
StyI CCWWGG 1 cut(s) 415
TaaI ACNGT 4 cut(s) 200, 224, 328, 500
TaiI ACGT 2 cut(s) 584, 924
TaqI TCGA 4 cut(s) 123, 246, 332, 874
TasI AATT 2 cut(s) 286, 423
TfiI GAWTC 1 cut(s) 714
Tru1I TTAA 2 cut(s) 297, 663
Tru9I TTAA 2 cut(s) 297, 663
TscAI CASTG 4 cut(s) 310, 333, 439, 834
TseFI GTSAC 3 cut(s) 89, 140, 868
Tsp45I GTSAC 3 cut(s) 89, 140, 868
TspDTI ATGAA 2 cut(s) 416, 807
TspGWI ACGGA 2 cut(s) 648, 723
TspRI CASTG 4 cut(s) 310, 333, 439, 834
Tth111I GACNNNGTC 1 cut(s) 739
Vha464I CTTAAG 1 cut(s) 296
XagI CCTNNNNNAGG 1 cut(s) 257
XcmI CCANNNNNNNNNTGG 1 cut(s) 310
XhoI CTCGAG 2 cut(s) 122, 873
XspI CTAG 1 cut(s) 815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.