Rw4G008170

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000309
Physical Location & Seq
Forward (+)
17714582 .. 17715520
939 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G008170.1

Sequence Viewer

Length: 939 bp
ATGGCCTCAAAAACTCTCCCTCCCTTCACAATACTCACTCTCAATCTCCTTTTCATTCTCCTTTCTCTCCTCTCTTGTCCAACTTCATCAGAATCCCAGAGTCACACAAACTCATCATCATCGCCATTCGAGTTTCTGGAGCATCTCAAGGGGTCTCACAAGGGTGATCACATTCAAGGCATCCACGACCTCAAACTCTACCTTGAGAAATTCGGTTACTTAAGCTACGGAAACGACAAGAACCACACTCATGCTGACGATGACGATTTCGACGACGTCGTTGAGTCGGCCGTCAAGGACTACCAGCACTACTACCACCTCAGAGCCACCGGAACCCTTGACGGCAAAACCGTTTCCAAGATGATGGCGCCCCGTTGCGGTGTGCCAGATCATATTGGCGTCCACAAAATCAACAATAGTAAAAAGGATCACATTAATGAAGTCTCTCACTATTCTTTCTTCAGCAAAAAGCAAAAATGGCCTCCTGCCAAGTACCAGCTCACCTACGCTTTCGCTCCGGGCACTCCATCCGAAGCCAAGACTCCGGTTGCACAAGCTTTTCAAACGTGGGCGGCCAGCACTCACTTCAAGTTCAGACGACACAAAGATTACAAGAAGGCTGACATAAAGATCAGTTTCGAACAAGGGGATCACGGAGATGGGAGCTCATTTGACGGCGCCGGTGGGATTCTAGCTCATGCTTATGCACCGACTTATGGAAAGCTCCACTACGACGCCGACGAGCCCTGGTCCGTCGGCGCCTTGGAAGGTTATTATGACTTGGAGACAGTGGCTTTGCATGAGATTGGTCACCTTCTCGGACTTGCTCATAGCTCGGTTGAGGGAGCTATCATGTACCCTTACATCTCTTCTGGAGTTACCAAGCTGGATTTGCATCATGACGATATTCACGGAATTAGAGCTTTATACAAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

312

Amino Acids

34.78

Weight (kDa)

6.37

Isoelectric Point (pI)

42.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 61 - 121 4.8e-10 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 160 - 310 3.6e-48 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 279
AccB1I GGYRCC 3 cut(s) 367, 677, 758
AciI CCGC 2 cut(s) 378, 572
AclWI GGATC 2 cut(s) 435, 657
AcoI YGGCCR 2 cut(s) 288, 573
AcsI RAATTY 1 cut(s) 209
AcuI CTGAAG 1 cut(s) 445
AcyI GRCGYC 6 cut(s) 276, 368, 399, 678, 735, 759
AfaI GTAC 2 cut(s) 494, 857
AfiI CCNNNNNNNGG 2 cut(s) 377, 716
AflII CTTAAG 1 cut(s) 220
AgsI TTSAA 3 cut(s) 176, 563, 589
AjnI CCWGG 1 cut(s) 746
AleI CACNNNNGTG 1 cut(s) 162
Alw21I GWGCWC 1 cut(s) 668
Alw26I GTCTC 3 cut(s) 159, 448, 779
AlwI GGATC 2 cut(s) 435, 657
AoxI GGCC 4 cut(s) 3, 288, 479, 573
ApoI RAATTY 1 cut(s) 209
ArsI GACNNNNNNTTYG 2 cut(s) 778, 810
AseI ATTAAT 1 cut(s) 435
AspLEI GCGC 3 cut(s) 370, 680, 761
AspS9I GGNCC 1 cut(s) 750
AsuC2I CCSGG 1 cut(s) 519
AsuHPI GGTGA 3 cut(s) 176, 493, 803
AsuII TTCGAA 1 cut(s) 639
AvaII GGWCC 1 cut(s) 750
BaeGI GKGCMC 1 cut(s) 524
BanI GGYRCC 3 cut(s) 367, 677, 758
BanII GRGCYC 2 cut(s) 668, 747
Bbv12I GWGCWC 1 cut(s) 668
BccI CCATC 3 cut(s) 358, 535, 653
BceAI ACGGC 3 cut(s) 275, 358, 691
BciT130I CCWGG 1 cut(s) 748
BclI TGATCA 1 cut(s) 166
BcnI CCSGG 1 cut(s) 519
BcoDI GTCTC 3 cut(s) 159, 448, 779
BfaI CTAG 1 cut(s) 692
BfoI RGCGCY 3 cut(s) 371, 681, 762
BfrI CTTAAG 1 cut(s) 220
BisI GCNGC 1 cut(s) 573
BlsI GCNGC 1 cut(s) 574
Bme1390I CCNGG 2 cut(s) 519, 748
Bme18I GGWCC 1 cut(s) 750
BmgT120I GGNCC 1 cut(s) 750
BmiI GGNNCC 4 cut(s) 334, 369, 679, 760
BmrFI CCNGG 2 cut(s) 519, 748
BmsI GCATC 3 cut(s) 151, 189, 904
BpmI CTGGAG 2 cut(s) 158, 894
Bpu14I TTCGAA 1 cut(s) 639
BpuEI CTTGAG 2 cut(s) 131, 224
BpuMI CCSGG 1 cut(s) 519
BsaBI GATNNNNATC 1 cut(s) 894
BsaHI GRCGYC 6 cut(s) 276, 368, 399, 678, 735, 759
BsaI GGTCTC 1 cut(s) 159
BsaJI CCNNGG 2 cut(s) 746, 762
BsaWI WCCGGW 2 cut(s) 329, 544
BsaXI ACNNNNNCTCC 1 cut(s) 34
Bsc4I CCNNNNNNNGG 2 cut(s) 377, 716
Bse118I RCCGGY 1 cut(s) 680
Bse8I GATNNNNATC 1 cut(s) 894
BseBI CCWGG 1 cut(s) 748
BseDI CCNNGG 2 cut(s) 746, 762
BseGI GGATG 2 cut(s) 180, 527
BseJI GATNNNNATC 1 cut(s) 894
BseLI CCNNNNNNNGG 2 cut(s) 377, 716
BseMII CTCAG 1 cut(s) 334
BseRI GAGGAG 1 cut(s) 59
BseSI GKGCMC 1 cut(s) 524
BseX3I CGGCCG 1 cut(s) 288
Bsh1285I CGRYCG 1 cut(s) 291
BshFI GGCC 4 cut(s) 5, 290, 481, 575
BshNI GGYRCC 3 cut(s) 367, 677, 758
BsiEI CGRYCG 1 cut(s) 291
BsiHKAI GWGCWC 1 cut(s) 668
BsiSI CCGG 4 cut(s) 330, 518, 545, 681
BslI CCNNNNNNNGG 2 cut(s) 377, 716
BsmAI GTCTC 3 cut(s) 159, 448, 779
BsnI GGCC 4 cut(s) 5, 290, 481, 575
Bso31I GGTCTC 1 cut(s) 159
Bsp119I TTCGAA 1 cut(s) 639
Bsp1286I GDGCHC 3 cut(s) 524, 668, 747
Bsp143I GATC 5 cut(s) 166, 388, 427, 630, 649
BspACI CCGC 2 cut(s) 378, 572
BspANI GGCC 4 cut(s) 5, 290, 481, 575
BspCNI CTCAG 1 cut(s) 333
BspHI TCATGA 1 cut(s) 898
BspLI GGNNCC 4 cut(s) 334, 369, 679, 760
BspPI GGATC 2 cut(s) 435, 657
BspT104I TTCGAA 1 cut(s) 639
BspT107I GGYRCC 3 cut(s) 367, 677, 758
BspTI CTTAAG 1 cut(s) 220
BspTNI GGTCTC 1 cut(s) 159
BsrFI RCCGGY 1 cut(s) 680
BssAI RCCGGY 1 cut(s) 680
BssECI CCNNGG 2 cut(s) 746, 762
BssMI GATC 5 cut(s) 166, 388, 427, 630, 649
BssNI GRCGYC 6 cut(s) 276, 368, 399, 678, 735, 759
BssT1I CCWWGG 1 cut(s) 762
Bst2UI CCWGG 1 cut(s) 748
Bst4CI ACNGT 2 cut(s) 352, 790
Bst6I CTCTTC 1 cut(s) 874
BstACI GRCGYC 6 cut(s) 276, 368, 399, 678, 735, 759
BstAFI CTTAAG 1 cut(s) 220
BstBI TTCGAA 1 cut(s) 639
BstC8I GCNNGC 1 cut(s) 577
BstDEI CTNAG 1 cut(s) 320
BstEII GGTNACC 1 cut(s) 809
BstF5I GGATG 2 cut(s) 180, 527
BstH2I RGCGCY 3 cut(s) 371, 681, 762
BstHHI GCGC 3 cut(s) 370, 680, 761
BstKTI GATC 5 cut(s) 169, 391, 430, 633, 652
BstMAI GTCTC 3 cut(s) 159, 448, 779
BstMBI GATC 5 cut(s) 166, 388, 427, 630, 649
BstMCI CGRYCG 1 cut(s) 291
BstMWI GCNNNNNNNGC 2 cut(s) 478, 892
BstNI CCWGG 1 cut(s) 748
BstPI GGTNACC 1 cut(s) 809
BstSCI CCNGG 2 cut(s) 517, 746
BstSLI GKGCMC 1 cut(s) 524
BstXI CCANNNNNNTGG 1 cut(s) 364
BstZI CGGCCG 1 cut(s) 288
BsuRI GGCC 4 cut(s) 5, 290, 481, 575
BtgZI GCGATG 1 cut(s) 105
BtsCI GGATG 2 cut(s) 180, 527
BtsIMutI CAGTG 1 cut(s) 795
Cac8I GCNNGC 1 cut(s) 577
CciI TCATGA 1 cut(s) 898
CfoI GCGC 3 cut(s) 370, 680, 761
Cfr10I RCCGGY 1 cut(s) 680
Cfr13I GGNCC 1 cut(s) 750
CseI GACGC 2 cut(s) 388, 743
Csp6I GTAC 2 cut(s) 493, 856
CviAII CATG 5 cut(s) 251, 698, 800, 853, 899
CviQI GTAC 2 cut(s) 493, 856
DdeI CTNAG 1 cut(s) 320
DinI GGCGCC 3 cut(s) 369, 679, 760
DpnI GATC 5 cut(s) 168, 390, 429, 632, 651
DpnII GATC 5 cut(s) 166, 388, 427, 630, 649
EaeI YGGCCR 2 cut(s) 288, 573
EagI CGGCCG 1 cut(s) 288
Eam1104I CTCTTC 1 cut(s) 874
EarI CTCTTC 1 cut(s) 874
Ecl136II GAGCTC 1 cut(s) 666
EclXI CGGCCG 1 cut(s) 288
Eco130I CCWWGG 1 cut(s) 762
Eco24I GRGCYC 2 cut(s) 668, 747
Eco31I GGTCTC 1 cut(s) 159
Eco47I GGWCC 1 cut(s) 750
Eco52I CGGCCG 1 cut(s) 288
Eco53kI GAGCTC 1 cut(s) 666
Eco57I CTGAAG 1 cut(s) 445
Eco91I GGTNACC 1 cut(s) 809
EcoICRI GAGCTC 1 cut(s) 666
EcoO65I GGTNACC 1 cut(s) 809
EcoRII CCWGG 1 cut(s) 746
EcoT14I CCWWGG 1 cut(s) 762
EcoT38I GRGCYC 2 cut(s) 668, 747
EgeI GGCGCC 3 cut(s) 369, 679, 760
EheI GGCGCC 3 cut(s) 369, 679, 760
ErhI CCWWGG 1 cut(s) 762
FaeI CATG 5 cut(s) 254, 701, 803, 856, 902
FatI CATG 5 cut(s) 250, 697, 799, 852, 898
FbaI TGATCA 1 cut(s) 166
Fnu4HI GCNGC 1 cut(s) 573
FokI GGATG 2 cut(s) 167, 514
FriOI GRGCYC 2 cut(s) 668, 747
Fsp4HI GCNGC 1 cut(s) 573
FspBI CTAG 1 cut(s) 692
GlaI GCGC 3 cut(s) 369, 679, 760
GluI GCNGC 1 cut(s) 573
GsuI CTGGAG 2 cut(s) 158, 894
HaeII RGCGCY 3 cut(s) 371, 681, 762
HaeIII GGCC 4 cut(s) 5, 290, 481, 575
HapII CCGG 4 cut(s) 330, 518, 545, 681
HgaI GACGC 2 cut(s) 388, 743
HhaI GCGC 3 cut(s) 370, 680, 761
Hin1I GRCGYC 6 cut(s) 276, 368, 399, 678, 735, 759
Hin1II CATG 5 cut(s) 254, 701, 803, 856, 902
Hin6I GCGC 3 cut(s) 368, 678, 759
HinP1I GCGC 3 cut(s) 368, 678, 759
HindIII AAGCTT 1 cut(s) 555
HinfI GANTC 5 cut(s) 92, 100, 284, 541, 688
HpaII CCGG 4 cut(s) 330, 518, 545, 681
HphI GGTGA 3 cut(s) 176, 493, 803
Hpy166II GTNNAC 1 cut(s) 403
Hpy188I TCNGA 5 cut(s) 91, 323, 532, 596, 821
Hpy188III TCNNGA 4 cut(s) 137, 873, 899, 936
Hpy8I GTNNAC 1 cut(s) 403
Hpy99I CGWCG 6 cut(s) 275, 278, 281, 737, 743, 758
HpyAV CCTTC 4 cut(s) 34, 610, 761, 824
HpyCH4III ACNGT 2 cut(s) 352, 790
HpyCH4IV ACGT 2 cut(s) 276, 566
HpyCH4V TGCA 4 cut(s) 551, 707, 799, 895
HpyF10VI GCNNNNNNNGC 2 cut(s) 478, 892
HpyF3I CTNAG 1 cut(s) 320
HpySE526I ACGT 2 cut(s) 276, 566
Hsp92I GRCGYC 6 cut(s) 276, 368, 399, 678, 735, 759
Hsp92II CATG 5 cut(s) 254, 701, 803, 856, 902
HspAI GCGC 3 cut(s) 368, 678, 759
KasI GGCGCC 3 cut(s) 367, 677, 758
Ksp22I TGATCA 1 cut(s) 166
Kzo9I GATC 5 cut(s) 166, 388, 427, 630, 649
LmnI GCTCC 5 cut(s) 139, 520, 663, 729, 845
LweI GCATC 3 cut(s) 151, 189, 904
MaeI CTAG 1 cut(s) 692
MaeII ACGT 2 cut(s) 276, 566
MaeIII GTNAC 4 cut(s) 101, 215, 809, 877
MalI GATC 5 cut(s) 168, 390, 429, 632, 651
MboI GATC 5 cut(s) 166, 388, 427, 630, 649
MboII GAAGA 2 cut(s) 451, 861
MhlI GDGCHC 3 cut(s) 524, 668, 747
MluCI AATT 2 cut(s) 209, 915
Mly113I GGCGCC 3 cut(s) 368, 678, 759
MlyI GAGTC 3 cut(s) 109, 293, 535
MmeI TCCRAC 1 cut(s) 104
MnlI CCTC 7 cut(s) 16, 30, 80, 200, 329, 492, 835
MseI TTAA 2 cut(s) 221, 435
MslI CAYNNNNRTG 5 cut(s) 162, 249, 435, 657, 702
MspCI CTTAAG 1 cut(s) 220
MspI CCGG 4 cut(s) 330, 518, 545, 681
MspR9I CCNGG 2 cut(s) 519, 748
MvaI CCWGG 1 cut(s) 748
MwoI GCNNNNNNNGC 2 cut(s) 478, 892
NarI GGCGCC 3 cut(s) 368, 678, 759
NciI CCSGG 1 cut(s) 519
NdeII GATC 5 cut(s) 166, 388, 427, 630, 649
NlaIII CATG 5 cut(s) 254, 701, 803, 856, 902
NlaIV GGNNCC 4 cut(s) 334, 369, 679, 760
NmuCI GTSAC 2 cut(s) 101, 809
NspV TTCGAA 1 cut(s) 639
OliI CACNNNNGTG 1 cut(s) 162
PagI TCATGA 1 cut(s) 898
PcsI WCGNNNNNNNCGW 4 cut(s) 270, 276, 348, 738
PfeI GAWTC 2 cut(s) 92, 688
PflFI GACNNNGTC 1 cut(s) 275
PkrI GCNGC 1 cut(s) 574
PleI GAGTC 3 cut(s) 108, 292, 535
PluTI GGCGCC 3 cut(s) 371, 681, 762
PpsI GAGTC 3 cut(s) 108, 292, 535
PshBI ATTAAT 1 cut(s) 435
Psp124BI GAGCTC 1 cut(s) 668
Psp6I CCWGG 1 cut(s) 746
PspEI GGTNACC 1 cut(s) 809
PspGI CCWGG 1 cut(s) 746
PspN4I GGNNCC 4 cut(s) 334, 369, 679, 760
PspPI GGNCC 1 cut(s) 750
PsyI GACNNNGTC 1 cut(s) 275
RsaI GTAC 2 cut(s) 494, 857
RsaNI GTAC 2 cut(s) 493, 856
RseI CAYNNNNRTG 5 cut(s) 162, 249, 435, 657, 702
SacI GAGCTC 1 cut(s) 668
SaqAI TTAA 2 cut(s) 221, 435
SatI GCNGC 1 cut(s) 573
Sau3AI GATC 5 cut(s) 166, 388, 427, 630, 649
Sau96I GGNCC 1 cut(s) 750
SchI GAGTC 3 cut(s) 109, 293, 535
ScrFI CCNGG 2 cut(s) 519, 748
SduI GDGCHC 3 cut(s) 524, 668, 747
SfaNI GCATC 3 cut(s) 151, 189, 904
SfoI GGCGCC 3 cut(s) 369, 679, 760
SfuI TTCGAA 1 cut(s) 639
SgrAI CRCCGGYG 1 cut(s) 680
SinI GGWCC 1 cut(s) 750
SmiMI CAYNNNNRTG 5 cut(s) 162, 249, 435, 657, 702
SmlI CTYRAG 3 cut(s) 146, 203, 220
SmoI CTYRAG 3 cut(s) 146, 203, 220
Sse9I AATT 2 cut(s) 209, 915
SsiI CCGC 2 cut(s) 378, 572
SspDI GGCGCC 3 cut(s) 367, 677, 758
SspMI CTAG 1 cut(s) 692
SstI GAGCTC 1 cut(s) 668
StyD4I CCNGG 2 cut(s) 517, 746
StyI CCWWGG 1 cut(s) 762
TaaI ACNGT 2 cut(s) 352, 790
TaiI ACGT 2 cut(s) 279, 569
TaqI TCGA 3 cut(s) 129, 270, 639
TasI AATT 2 cut(s) 209, 915
TauI GCSGC 1 cut(s) 575
TfiI GAWTC 2 cut(s) 92, 688
Tru1I TTAA 2 cut(s) 221, 435
Tru9I TTAA 2 cut(s) 221, 435
TscAI CASTG 1 cut(s) 795
TseFI GTSAC 2 cut(s) 101, 809
Tsp45I GTSAC 2 cut(s) 101, 809
TspDTI ATGAA 3 cut(s) 43, 75, 453
TspGWI ACGGA 4 cut(s) 243, 669, 742, 927
TspRI CASTG 1 cut(s) 795
Tth111I GACNNNGTC 1 cut(s) 275
Vha464I CTTAAG 1 cut(s) 220
VpaK11BI GGWCC 1 cut(s) 750
VspI ATTAAT 1 cut(s) 435
XapI RAATTY 1 cut(s) 209
XspI CTAG 1 cut(s) 692
ZraI GACGTC 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.