Rroxscaffold_2G00081540

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000309
Physical Location & Seq
Forward (+)
4612650 .. 4613860
1211 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00081540.1

Sequence Viewer

Length: 927 bp
ATGGCATCCAAATCTAATCTTTCTCTCTTCACTGTCACTCTCCTCCTTGCCCTTCTTACTCTCCTCTCTTATGCAACTGCTTCTCATCCCCACAACAAGAGCTCTTCCCCATTTGAGTTCCTTGAGCATCTCAAGGGTTGCCACAAAGGTGATAAGGTCAAGGGCATCAATGATCTCAAAAAATACCTAGAAAAGTACGGTTACTTGAACTACAAGAACCACAGTCATTCGGACGACGATGATTTTGATGACCTTTTGGAGGAAGCCATCAAGACTTACCAGGTCAATTACCACCTCAAGTCCACCGGAACACTGGATGGCAAAACAGTGTCGAAGATGATGACACCTCGTTGTGGCATGCCGGATATCATCAATGGCACCTCGTCCATGAGATCAGCCAAGAAAAGGCACCACCATGGTTCTATTCATACCACTGCTCACTACTCATTTTTCCAGGGAAATCCAAAATGGCCTTCTTCTAAATATCATCTCACTTACGGTTTTCTCCCCAACACCCCATCCGAAGCCACGGGCGCTGTCGCACGGGCTTTCGCAACATGGGCTAGAAACACTCACTTCACGTTCAGCCAGTCCCAAAGTTACCAGAATGCGGATTTGAAGATTAGTTTTCATAAGGGTGATCATGGAGATGGGAATTCATTTGATGGCCCGGGCGGGGTCCTAGCCCATGCTTTTAGCCCGTCGGATGGAAGATTCCACTACGACGCAGATGAGCCTTGGTCTGTGGGTGCTACACCAGGTGCTTATGACTTGGAGACTGTGGCTCTGCATGAAATAGGGCACCTTCTGGGACTTGGACATAGCTCAGTGGAGGGAGCTATCATGGCCCCAGCTATTCCCCCAGGAGTGACTAAAAGTTTGCATGGGGACGATGTTCAAGGAATCAAGGTTTTATACAACGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

308

Amino Acids

33.75

Weight (kDa)

6.89

Isoelectric Point (pI)

30.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 56 - 113 2.7e-10 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 156 - 306 2.2e-46 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 377, 408, 801
AciI CCGC 2 cut(s) 611, 675
AcsI RAATTY 1 cut(s) 655
AdeI CACNNNGTG 1 cut(s) 761
AfaI GTAC 1 cut(s) 197
AfiI CCNNNNNNNGG 6 cut(s) 259, 353, 405, 610, 676, 707
AgsI TTSAA 3 cut(s) 208, 619, 899
AjnI CCWGG 4 cut(s) 279, 453, 757, 862
AjuI GAANNNNNNNTTGG 2 cut(s) 457, 489
AleI CACNNNNGTG 1 cut(s) 147
AluBI AGCT 4 cut(s) 102, 825, 839, 854
AluI AGCT 4 cut(s) 102, 825, 839, 854
Alw21I GWGCWC 1 cut(s) 104
Alw26I GTCTC 1 cut(s) 770
Ama87I CYCGRG 1 cut(s) 670
AoxI GGCC 3 cut(s) 470, 667, 846
ApoI RAATTY 1 cut(s) 655
ArsI GACNNNNNNTTYG 4 cut(s) 227, 259, 862, 894
AspLEI GCGC 1 cut(s) 536
AspS9I GGNCC 3 cut(s) 668, 679, 847
AsuC2I CCSGG 2 cut(s) 671, 672
AsuHPI GGTGA 2 cut(s) 161, 650
AvaI CYCGRG 1 cut(s) 670
AvaII GGWCC 1 cut(s) 679
BaeGI GKGCMC 1 cut(s) 804
BanI GGYRCC 3 cut(s) 377, 408, 801
BanII GRGCYC 1 cut(s) 104
Bbv12I GWGCWC 1 cut(s) 104
BccI CCATC 6 cut(s) 275, 311, 526, 644, 659, 701
BciT130I CCWGG 4 cut(s) 281, 455, 759, 864
BclI TGATCA 1 cut(s) 640
BcnI CCSGG 2 cut(s) 671, 672
BcoDI GTCTC 1 cut(s) 770
BfaI CTAG 3 cut(s) 188, 564, 683
BfoI RGCGCY 1 cut(s) 537
Bme1390I CCNGG 6 cut(s) 281, 455, 671, 672, 759, 864
Bme18I GGWCC 1 cut(s) 679
BmeT110I CYCGRG 1 cut(s) 670
BmgT120I GGNCC 3 cut(s) 668, 679, 847
BmiI GGNNCC 5 cut(s) 379, 410, 680, 803, 849
BmrFI CCNGG 6 cut(s) 281, 455, 671, 672, 759, 864
BmsI GCATC 3 cut(s) 14, 136, 174
BpuEI CTTGAG 3 cut(s) 116, 143, 281
BpuMI CCSGG 2 cut(s) 671, 672
BsaJI CCNNGG 6 cut(s) 415, 454, 528, 670, 737, 862
BsaWI WCCGGW 1 cut(s) 305
Bsc4I CCNNNNNNNGG 6 cut(s) 259, 353, 405, 610, 676, 707
Bse1I ACTGG 2 cut(s) 318, 589
BseBI CCWGG 4 cut(s) 281, 455, 759, 864
BseDI CCNNGG 6 cut(s) 415, 454, 528, 670, 737, 862
BseGI GGATG 5 cut(s) 5, 85, 322, 518, 712
BseLI CCNNNNNNNGG 6 cut(s) 259, 353, 405, 610, 676, 707
BseMII CTCAG 1 cut(s) 840
BseNI ACTGG 2 cut(s) 318, 589
BseRI GAGGAG 2 cut(s) 32, 53
BseSI GKGCMC 1 cut(s) 804
BseYI CCCAGC 1 cut(s) 850
BshFI GGCC 3 cut(s) 472, 669, 848
BshNI GGYRCC 3 cut(s) 377, 408, 801
BsiHKAI GWGCWC 1 cut(s) 104
BsiHKCI CYCGRG 1 cut(s) 670
BsiSI CCGG 3 cut(s) 306, 362, 671
BslFI GGGAC 3 cut(s) 577, 825, 902
BslI CCNNNNNNNGG 6 cut(s) 259, 353, 405, 610, 676, 707
BsmAI GTCTC 1 cut(s) 770
BsmFI GGGAC 3 cut(s) 577, 825, 902
BsmI GAATGC 1 cut(s) 613
BsnI GGCC 3 cut(s) 472, 669, 848
BsoBI CYCGRG 1 cut(s) 670
Bsp1286I GDGCHC 2 cut(s) 104, 804
Bsp143I GATC 3 cut(s) 172, 392, 640
Bsp19I CCATGG 1 cut(s) 415
BspACI CCGC 2 cut(s) 611, 675
BspANI GGCC 3 cut(s) 472, 669, 848
BspCNI CTCAG 1 cut(s) 839
BspLI GGNNCC 5 cut(s) 379, 410, 680, 803, 849
BspQI GCTCTTC 1 cut(s) 109
BspT107I GGYRCC 3 cut(s) 377, 408, 801
BsrI ACTGG 2 cut(s) 318, 589
BssECI CCNNGG 6 cut(s) 415, 454, 528, 670, 737, 862
BssMI GATC 3 cut(s) 172, 392, 640
BssT1I CCWWGG 2 cut(s) 415, 737
Bst2UI CCWGG 4 cut(s) 281, 455, 759, 864
Bst4CI ACNGT 6 cut(s) 34, 200, 224, 328, 500, 781
Bst6I CTCTTC 2 cut(s) 32, 109
BstC8I GCNNGC 1 cut(s) 359
BstDEI CTNAG 1 cut(s) 826
BstDSI CCRYGG 2 cut(s) 415, 528
BstENI CCTNNNNNAGG 1 cut(s) 257
BstF5I GGATG 5 cut(s) 5, 85, 322, 518, 712
BstH2I RGCGCY 1 cut(s) 537
BstHHI GCGC 1 cut(s) 536
BstKTI GATC 3 cut(s) 175, 395, 643
BstMAI GTCTC 1 cut(s) 770
BstMBI GATC 3 cut(s) 172, 392, 640
BstMWI GCNNNNNNNGC 3 cut(s) 533, 560, 845
BstNI CCWGG 4 cut(s) 281, 455, 759, 864
BstNSI RCATGY 1 cut(s) 361
BstSCI CCNGG 6 cut(s) 279, 453, 669, 670, 757, 862
BstSLI GKGCMC 1 cut(s) 804
BsuRI GGCC 3 cut(s) 472, 669, 848
BtgI CCRYGG 2 cut(s) 415, 528
BtsCI GGATG 5 cut(s) 5, 85, 322, 518, 712
BtsI GCAGTG 1 cut(s) 432
BtsIMutI CAGTG 5 cut(s) 30, 311, 333, 432, 834
Cac8I GCNNGC 1 cut(s) 359
CfoI GCGC 1 cut(s) 536
Cfr13I GGNCC 3 cut(s) 668, 679, 847
Cfr9I CCCGGG 1 cut(s) 670
CseI GACGC 1 cut(s) 734
CsiI ACCWGGT 2 cut(s) 279, 757
Csp6I GTAC 1 cut(s) 196
CviAII CATG 9 cut(s) 358, 388, 416, 558, 644, 689, 791, 844, 884
CviQI GTAC 1 cut(s) 196
DdeI CTNAG 1 cut(s) 826
DpnI GATC 3 cut(s) 174, 394, 642
DpnII GATC 3 cut(s) 172, 392, 640
DraIII CACNNNGTG 1 cut(s) 761
Eam1104I CTCTTC 2 cut(s) 32, 109
EarI CTCTTC 2 cut(s) 32, 109
Ecl136II GAGCTC 1 cut(s) 102
Eco130I CCWWGG 2 cut(s) 415, 737
Eco24I GRGCYC 1 cut(s) 104
Eco32I GATATC 1 cut(s) 367
Eco47I GGWCC 1 cut(s) 679
Eco53kI GAGCTC 1 cut(s) 102
Eco88I CYCGRG 1 cut(s) 670
EcoICRI GAGCTC 1 cut(s) 102
EcoNI CCTNNNNNAGG 1 cut(s) 257
EcoO109I RGGNCCY 1 cut(s) 679
EcoRI GAATTC 1 cut(s) 655
EcoRII CCWGG 4 cut(s) 279, 453, 757, 862
EcoRV GATATC 1 cut(s) 367
EcoT14I CCWWGG 2 cut(s) 415, 737
EcoT38I GRGCYC 1 cut(s) 104
ErhI CCWWGG 2 cut(s) 415, 737
FaeI CATG 9 cut(s) 361, 391, 419, 561, 647, 692, 794, 847, 887
FaqI GGGAC 3 cut(s) 577, 825, 902
FatI CATG 9 cut(s) 357, 387, 415, 557, 643, 688, 790, 843, 883
FauI CCCGC 1 cut(s) 668
FbaI TGATCA 1 cut(s) 640
FokI GGATG 4 cut(s) 72, 329, 505, 719
FriOI GRGCYC 1 cut(s) 104
FspBI CTAG 3 cut(s) 188, 564, 683
GlaI GCGC 1 cut(s) 535
GsaI CCCAGC 1 cut(s) 854
HaeII RGCGCY 1 cut(s) 537
HaeIII GGCC 3 cut(s) 472, 669, 848
HapII CCGG 3 cut(s) 306, 362, 671
HgaI GACGC 1 cut(s) 734
HhaI GCGC 1 cut(s) 536
Hin1II CATG 9 cut(s) 361, 391, 419, 561, 647, 692, 794, 847, 887
Hin6I GCGC 1 cut(s) 534
HinP1I GCGC 1 cut(s) 534
HinfI GANTC 2 cut(s) 714, 903
HpaII CCGG 3 cut(s) 306, 362, 671
HphI GGTGA 2 cut(s) 161, 650
Hpy166II GTNNAC 1 cut(s) 303
Hpy188I TCNGA 4 cut(s) 232, 523, 706, 926
Hpy188III TCNNGA 1 cut(s) 271
Hpy8I GTNNAC 1 cut(s) 303
Hpy99I CGWCG 3 cut(s) 239, 706, 728
HpyAV CCTTC 3 cut(s) 62, 483, 815
HpyCH4III ACNGT 6 cut(s) 34, 200, 224, 328, 500, 781
HpyCH4IV ACGT 2 cut(s) 581, 921
HpyCH4V TGCA 3 cut(s) 74, 790, 883
HpyF10VI GCNNNNNNNGC 3 cut(s) 533, 560, 845
HpyF3I CTNAG 1 cut(s) 826
HpySE526I ACGT 2 cut(s) 581, 921
Hsp92II CATG 9 cut(s) 361, 391, 419, 561, 647, 692, 794, 847, 887
HspAI GCGC 1 cut(s) 534
Ksp22I TGATCA 1 cut(s) 640
Kzo9I GATC 3 cut(s) 172, 392, 640
LguI GCTCTTC 1 cut(s) 109
LmnI GCTCC 1 cut(s) 836
LweI GCATC 3 cut(s) 14, 136, 174
MabI ACCWGGT 2 cut(s) 279, 757
MaeI CTAG 3 cut(s) 188, 564, 683
MaeII ACGT 2 cut(s) 581, 921
MaeIII GTNAC 4 cut(s) 34, 200, 599, 868
MalI GATC 3 cut(s) 174, 394, 642
MboI GATC 3 cut(s) 172, 392, 640
MboII GAAGA 6 cut(s) 19, 96, 346, 468, 631, 723
MhlI GDGCHC 2 cut(s) 104, 804
MluCI AATT 2 cut(s) 286, 655
MmeI TCCRAC 1 cut(s) 684
MnlI CCTC 7 cut(s) 53, 74, 253, 305, 357, 391, 826
MslI CAYNNNNRTG 4 cut(s) 147, 414, 636, 648
MspI CCGG 3 cut(s) 306, 362, 671
MspR9I CCNGG 6 cut(s) 281, 455, 671, 672, 759, 864
Mva1269I GAATGC 1 cut(s) 613
MvaI CCWGG 4 cut(s) 281, 455, 759, 864
MwoI GCNNNNNNNGC 3 cut(s) 533, 560, 845
NciI CCSGG 2 cut(s) 671, 672
NcoI CCATGG 1 cut(s) 415
NdeII GATC 3 cut(s) 172, 392, 640
NlaIII CATG 9 cut(s) 361, 391, 419, 561, 647, 692, 794, 847, 887
NlaIV GGNNCC 5 cut(s) 379, 410, 680, 803, 849
NmuCI GTSAC 2 cut(s) 34, 868
NspI RCATGY 1 cut(s) 361
OliI CACNNNNGTG 1 cut(s) 147
PaeI GCATGC 1 cut(s) 361
PciSI GCTCTTC 1 cut(s) 109
PctI GAATGC 1 cut(s) 613
PfeI GAWTC 2 cut(s) 714, 903
PpuMI RGGWCCY 1 cut(s) 679
Psp124BI GAGCTC 1 cut(s) 104
Psp5II RGGWCCY 1 cut(s) 679
Psp6I CCWGG 4 cut(s) 279, 453, 757, 862
PspFI CCCAGC 1 cut(s) 850
PspGI CCWGG 4 cut(s) 279, 453, 757, 862
PspN4I GGNNCC 5 cut(s) 379, 410, 680, 803, 849
PspPI GGNCC 3 cut(s) 668, 679, 847
PspPPI RGGWCCY 1 cut(s) 679
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
RseI CAYNNNNRTG 4 cut(s) 147, 414, 636, 648
SacI GAGCTC 1 cut(s) 104
SapI GCTCTTC 1 cut(s) 109
Sau3AI GATC 3 cut(s) 172, 392, 640
Sau96I GGNCC 3 cut(s) 668, 679, 847
ScrFI CCNGG 6 cut(s) 281, 455, 671, 672, 759, 864
SduI GDGCHC 2 cut(s) 104, 804
SexAI ACCWGGT 2 cut(s) 279, 757
SfaNI GCATC 3 cut(s) 14, 136, 174
SinI GGWCC 1 cut(s) 679
SmaI CCCGGG 1 cut(s) 672
SmiMI CAYNNNNRTG 4 cut(s) 147, 414, 636, 648
SmlI CTYRAG 3 cut(s) 122, 131, 296
SmoI CTYRAG 3 cut(s) 122, 131, 296
SphI GCATGC 1 cut(s) 361
SrfI GCCCGGGC 1 cut(s) 672
Sse9I AATT 2 cut(s) 286, 655
SsiI CCGC 2 cut(s) 611, 675
SspMI CTAG 3 cut(s) 188, 564, 683
SstI GAGCTC 1 cut(s) 104
StyD4I CCNGG 6 cut(s) 279, 453, 669, 670, 757, 862
StyI CCWWGG 2 cut(s) 415, 737
TaaI ACNGT 6 cut(s) 34, 200, 224, 328, 500, 781
TaiI ACGT 2 cut(s) 584, 924
TaqI TCGA 1 cut(s) 332
TasI AATT 2 cut(s) 286, 655
TfiI GAWTC 2 cut(s) 714, 903
TscAI CASTG 5 cut(s) 37, 318, 333, 439, 834
TseFI GTSAC 2 cut(s) 34, 868
Tsp45I GTSAC 2 cut(s) 34, 868
TspDTI ATGAA 4 cut(s) 416, 620, 648, 807
TspMI CCCGGG 1 cut(s) 670
TspRI CASTG 5 cut(s) 37, 318, 333, 439, 834
VpaK11BI GGWCC 1 cut(s) 679
XagI CCTNNNNNAGG 1 cut(s) 257
XapI RAATTY 1 cut(s) 655
XceI RCATGY 1 cut(s) 361
XcmI CCANNNNNNNNNTGG 1 cut(s) 310
XmaI CCCGGG 1 cut(s) 670
XspI CTAG 3 cut(s) 188, 564, 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.