Rh2DG642800

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000309
Physical Location & Seq
Reverse (-)
86987986 .. 86990425
2440 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG642800.1

Sequence Viewer

Length: 765 bp
ATGGCACCAAAATCTGATCTTTCTCTTTTCACAGTCACTCTGCTCCTCCTCCTCCTCGCCCTCTTTTCTTTCCTCTCTAATGCAAGGGCAAACTCATCTTCGTTTGAGTTCCTTGAGCATCTTAAGGGTTGTCACAAAGGTGACAAGGTCAAGGGCATCAATGACCTCAAGAAGTACCTTCAAAAGTTCGGTTACTTAAACTACGAGAACCACAGACATTTCAACGATGATGATTTTGACGAGCTCTTGGAGGAAGCCGTCAAGACTTACCAGCTCAATTTCCACCTCAAGTCCACGGGAACATTGGACGACAAAACCGTATCAACGATGATGATGCCTCGTTGTGGTGTGCCCGATATCATCAATGGCACCACGTCCATGAGATCAGCCCAGATAAAGCGCCAGCACCGTGCAATTCATACCACCGTTCACTACTCATTTCCCGATGGAAATCTAAAATGGCCTTCTTCAAAATACCATCTGACTTACGGTTTTCTCGCCGGCACCCCATCCGAAGCCCCGGGTGCTGTCGCACAGGCTTTCTCAACATGGGCTAAGAACACTCACTTCAAATTCAGCAAGGCTCAAAATTACCAGAGTGCGGATTTGAAGGTTAGCTTTCATAAGGGTAATCATGGAGACGGGAATGCATTTGATGGGCCTGGCGGACAGCTGGCCCATGCTGCTTACCCTACGGAGGGAACATTGCACTACGACGCAGATGAGACTTGGTCTGTAGGTGCTAAACCAGGTGCCGTACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

254

Amino Acids

28.23

Weight (kDa)

7.84

Isoelectric Point (pI)

20.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 53 - 110 4.4e-10 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 153 - 248 3.3e-23 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 4 cut(s) 4, 368, 503, 752
AciI CCGC 2 cut(s) 602, 666
AcsI RAATTY 1 cut(s) 572
AfaI GTAC 2 cut(s) 176, 759
AfiI CCNNNNNNNGG 4 cut(s) 344, 601, 697, 698
AflII CTTAAG 1 cut(s) 122
AgsI TTSAA 5 cut(s) 182, 223, 471, 571, 610
AjiI CACGTC 1 cut(s) 375
AjnI CCWGG 2 cut(s) 661, 748
AleI CACNNNNGTG 1 cut(s) 138
AluBI AGCT 4 cut(s) 244, 274, 618, 673
AluI AGCT 4 cut(s) 244, 274, 618, 673
Alw21I GWGCWC 1 cut(s) 246
Alw26I GTCTC 2 cut(s) 633, 719
Ama87I CYCGRG 1 cut(s) 520
AoxI GGCC 3 cut(s) 461, 659, 675
ApeKI GCWGC 1 cut(s) 683
ApoI RAATTY 1 cut(s) 572
AspLEI GCGC 1 cut(s) 402
AspS9I GGNCC 2 cut(s) 659, 676
AsuC2I CCSGG 2 cut(s) 521, 522
AsuHPI GGTGA 1 cut(s) 152
AvaI CYCGRG 1 cut(s) 520
BaeGI GKGCMC 1 cut(s) 354
BanI GGYRCC 4 cut(s) 4, 368, 503, 752
BanII GRGCYC 1 cut(s) 246
Bbv12I GWGCWC 1 cut(s) 246
BbvI GCAGC 1 cut(s) 670
BccI CCATC 4 cut(s) 440, 486, 517, 650
BceAI ACGGC 2 cut(s) 242, 740
BcgI CGANNNNNNTGC 2 cut(s) 316, 350
BciT130I CCWGG 2 cut(s) 663, 750
BcnI CCSGG 2 cut(s) 521, 522
BcoDI GTCTC 2 cut(s) 633, 719
BfmI CTRYAG 1 cut(s) 735
BfoI RGCGCY 1 cut(s) 403
BfrI CTTAAG 1 cut(s) 122
BisI GCNGC 1 cut(s) 684
BlsI GCNGC 1 cut(s) 685
Bme1390I CCNGG 4 cut(s) 521, 522, 663, 750
BmeT110I CYCGRG 1 cut(s) 520
BmgBI CACGTC 1 cut(s) 375
BmgT120I GGNCC 2 cut(s) 659, 676
BmiI GGNNCC 4 cut(s) 6, 370, 505, 754
BmrFI CCNGG 4 cut(s) 521, 522, 663, 750
BmsI GCATC 3 cut(s) 127, 165, 324
BpuEI CTTGAG 3 cut(s) 134, 152, 272
BpuMI CCSGG 2 cut(s) 521, 522
BsaBI GATNNNNATC 1 cut(s) 450
BsaJI CCNNGG 3 cut(s) 294, 519, 520
Bsc4I CCNNNNNNNGG 4 cut(s) 344, 601, 697, 698
Bse118I RCCGGY 1 cut(s) 500
Bse3DI GCAATG 1 cut(s) 704
Bse8I GATNNNNATC 1 cut(s) 450
BseBI CCWGG 2 cut(s) 663, 750
BseDI CCNNGG 3 cut(s) 294, 519, 520
BseGI GGATG 1 cut(s) 509
BseJI GATNNNNATC 1 cut(s) 450
BseLI CCNNNNNNNGG 4 cut(s) 344, 601, 697, 698
BseMI GCAATG 1 cut(s) 704
BseRI GAGGAG 4 cut(s) 35, 38, 41, 44
BseSI GKGCMC 1 cut(s) 354
BseXI GCAGC 1 cut(s) 670
BshFI GGCC 3 cut(s) 463, 661, 677
BshNI GGYRCC 4 cut(s) 4, 368, 503, 752
BsiHKAI GWGCWC 1 cut(s) 246
BsiHKCI CYCGRG 1 cut(s) 520
BsiSI CCGG 2 cut(s) 501, 521
BslI CCNNNNNNNGG 4 cut(s) 344, 601, 697, 698
BsmAI GTCTC 2 cut(s) 633, 719
BsmBI CGTCTC 1 cut(s) 633
BsmI GAATGC 1 cut(s) 652
BsnI GGCC 3 cut(s) 463, 661, 677
BsoBI CYCGRG 1 cut(s) 520
Bsp1286I GDGCHC 2 cut(s) 246, 354
Bsp143I GATC 2 cut(s) 16, 383
BspACI CCGC 2 cut(s) 602, 666
BspANI GGCC 3 cut(s) 463, 661, 677
BspLI GGNNCC 4 cut(s) 6, 370, 505, 754
BspT107I GGYRCC 4 cut(s) 4, 368, 503, 752
BspTI CTTAAG 1 cut(s) 122
BsrDI GCAATG 1 cut(s) 704
BsrFI RCCGGY 1 cut(s) 500
BssAI RCCGGY 1 cut(s) 500
BssECI CCNNGG 3 cut(s) 294, 519, 520
BssMI GATC 2 cut(s) 16, 383
Bst2UI CCWGG 2 cut(s) 663, 750
Bst4CI ACNGT 5 cut(s) 34, 319, 410, 427, 491
BstAFI CTTAAG 1 cut(s) 122
BstC8I GCNNGC 3 cut(s) 404, 502, 675
BstDEI CTNAG 1 cut(s) 555
BstDSI CCRYGG 1 cut(s) 294
BstF5I GGATG 1 cut(s) 509
BstH2I RGCGCY 1 cut(s) 403
BstHHI GCGC 1 cut(s) 402
BstKTI GATC 2 cut(s) 19, 386
BstMAI GTCTC 2 cut(s) 633, 719
BstMBI GATC 2 cut(s) 16, 383
BstMWI GCNNNNNNNGC 2 cut(s) 524, 683
BstNI CCWGG 2 cut(s) 663, 750
BstSCI CCNGG 4 cut(s) 519, 520, 661, 748
BstSFI CTRYAG 1 cut(s) 735
BstSLI GKGCMC 1 cut(s) 354
BstV1I GCAGC 1 cut(s) 670
BsuRI GGCC 3 cut(s) 463, 661, 677
BtgI CCRYGG 1 cut(s) 294
BtrI CACGTC 1 cut(s) 375
BtsCI GGATG 1 cut(s) 509
Cac8I GCNNGC 3 cut(s) 404, 502, 675
CfoI GCGC 1 cut(s) 402
Cfr10I RCCGGY 1 cut(s) 500
Cfr13I GGNCC 2 cut(s) 659, 676
Cfr9I CCCGGG 1 cut(s) 520
CseI GACGC 1 cut(s) 725
CsiI ACCWGGT 1 cut(s) 748
Csp6I GTAC 2 cut(s) 175, 758
CviAII CATG 4 cut(s) 379, 549, 635, 680
CviQI GTAC 2 cut(s) 175, 758
DdeI CTNAG 1 cut(s) 555
DpnI GATC 2 cut(s) 18, 385
DpnII GATC 2 cut(s) 16, 383
EciI GGCGGA 1 cut(s) 681
Ecl136II GAGCTC 1 cut(s) 244
Eco24I GRGCYC 1 cut(s) 246
Eco32I GATATC 1 cut(s) 358
Eco53kI GAGCTC 1 cut(s) 244
Eco88I CYCGRG 1 cut(s) 520
EcoICRI GAGCTC 1 cut(s) 244
EcoRII CCWGG 2 cut(s) 661, 748
EcoRV GATATC 1 cut(s) 358
EcoT22I ATGCAT 1 cut(s) 652
EcoT38I GRGCYC 1 cut(s) 246
Esp3I CGTCTC 1 cut(s) 633
FaeI CATG 4 cut(s) 382, 552, 638, 683
FaiI YATR 6 cut(s) 380, 420, 550, 624, 636, 681
FalI AAGNNNNNCTT 2 cut(s) 602, 634
FatI CATG 4 cut(s) 378, 548, 634, 679
Fnu4HI GCNGC 1 cut(s) 684
FokI GGATG 1 cut(s) 496
FriOI GRGCYC 1 cut(s) 246
Fsp4HI GCNGC 1 cut(s) 684
GlaI GCGC 1 cut(s) 401
GluI GCNGC 1 cut(s) 684
HaeII RGCGCY 1 cut(s) 403
HaeIII GGCC 3 cut(s) 463, 661, 677
HapII CCGG 2 cut(s) 501, 521
HgaI GACGC 1 cut(s) 725
HhaI GCGC 1 cut(s) 402
Hin1II CATG 4 cut(s) 382, 552, 638, 683
Hin6I GCGC 1 cut(s) 400
HinP1I GCGC 1 cut(s) 400
HpaII CCGG 2 cut(s) 501, 521
HphI GGTGA 1 cut(s) 152
Hpy166II GTNNAC 2 cut(s) 294, 430
Hpy188I TCNGA 3 cut(s) 16, 483, 514
Hpy188III TCNNGA 3 cut(s) 169, 262, 443
Hpy8I GTNNAC 2 cut(s) 294, 430
Hpy99I CGWCG 1 cut(s) 719
HpyAV CCTTC 3 cut(s) 188, 474, 604
HpyCH4III ACNGT 5 cut(s) 34, 319, 410, 427, 491
HpyCH4IV ACGT 1 cut(s) 374
HpyCH4V TGCA 4 cut(s) 83, 413, 650, 709
HpyF10VI GCNNNNNNNGC 2 cut(s) 524, 683
HpyF3I CTNAG 1 cut(s) 555
HpySE526I ACGT 1 cut(s) 374
Hsp92II CATG 4 cut(s) 382, 552, 638, 683
HspAI GCGC 1 cut(s) 400
KroI GCCGGC 1 cut(s) 500
KroNI GCCGGC 1 cut(s) 502
Kzo9I GATC 2 cut(s) 16, 383
LmnI GCTCC 1 cut(s) 48
Lsp1109I GCAGC 1 cut(s) 670
LweI GCATC 3 cut(s) 127, 165, 324
MabI ACCWGGT 1 cut(s) 748
MaeII ACGT 1 cut(s) 374
MaeIII GTNAC 4 cut(s) 34, 131, 140, 191
MalI GATC 2 cut(s) 18, 385
MboI GATC 2 cut(s) 16, 383
MboII GAAGA 2 cut(s) 90, 459
MhlI GDGCHC 2 cut(s) 246, 354
MluCI AATT 4 cut(s) 277, 414, 572, 589
Mph1103I ATGCAT 1 cut(s) 652
MroNI GCCGGC 1 cut(s) 500
MseI TTAA 2 cut(s) 123, 197
MslI CAYNNNNRTG 2 cut(s) 138, 377
MspA1I CMGCKG 1 cut(s) 673
MspCI CTTAAG 1 cut(s) 122
MspI CCGG 2 cut(s) 501, 521
MspR9I CCNGG 4 cut(s) 521, 522, 663, 750
Mva1269I GAATGC 1 cut(s) 652
MvaI CCWGG 2 cut(s) 663, 750
MwoI GCNNNNNNNGC 2 cut(s) 524, 683
NaeI GCCGGC 1 cut(s) 502
NciI CCSGG 2 cut(s) 521, 522
NdeII GATC 2 cut(s) 16, 383
NgoMIV GCCGGC 1 cut(s) 500
NlaIII CATG 4 cut(s) 382, 552, 638, 683
NlaIV GGNNCC 4 cut(s) 6, 370, 505, 754
NmuCI GTSAC 3 cut(s) 34, 131, 140
NsiI ATGCAT 1 cut(s) 652
OliI CACNNNNGTG 1 cut(s) 138
PcsI WCGNNNNNNNCGW 1 cut(s) 315
PctI GAATGC 1 cut(s) 652
PdiI GCCGGC 1 cut(s) 502
PflFI GACNNNGTC 2 cut(s) 146, 730
PkrI GCNGC 1 cut(s) 685
Psp124BI GAGCTC 1 cut(s) 246
Psp6I CCWGG 2 cut(s) 661, 748
PspGI CCWGG 2 cut(s) 661, 748
PspN4I GGNNCC 4 cut(s) 6, 370, 505, 754
PspPI GGNCC 2 cut(s) 659, 676
PsyI GACNNNGTC 2 cut(s) 146, 730
PvuII CAGCTG 1 cut(s) 673
RsaI GTAC 2 cut(s) 176, 759
RsaNI GTAC 2 cut(s) 175, 758
RseI CAYNNNNRTG 2 cut(s) 138, 377
SacI GAGCTC 1 cut(s) 246
SaqAI TTAA 2 cut(s) 123, 197
SatI GCNGC 1 cut(s) 684
Sau3AI GATC 2 cut(s) 16, 383
Sau96I GGNCC 2 cut(s) 659, 676
ScrFI CCNGG 4 cut(s) 521, 522, 663, 750
SduI GDGCHC 2 cut(s) 246, 354
SexAI ACCWGGT 1 cut(s) 748
SfaNI GCATC 3 cut(s) 127, 165, 324
SfcI CTRYAG 1 cut(s) 735
SmaI CCCGGG 1 cut(s) 522
SmiMI CAYNNNNRTG 2 cut(s) 138, 377
SmlI CTYRAG 4 cut(s) 113, 122, 167, 287
SmoI CTYRAG 4 cut(s) 113, 122, 167, 287
Sse9I AATT 4 cut(s) 277, 414, 572, 589
SsiI CCGC 2 cut(s) 602, 666
SstI GAGCTC 1 cut(s) 246
StyD4I CCNGG 4 cut(s) 519, 520, 661, 748
TaaI ACNGT 5 cut(s) 34, 319, 410, 427, 491
TaiI ACGT 1 cut(s) 377
TasI AATT 4 cut(s) 277, 414, 572, 589
Tru1I TTAA 2 cut(s) 123, 197
Tru9I TTAA 2 cut(s) 123, 197
TseFI GTSAC 3 cut(s) 34, 131, 140
TseI GCWGC 1 cut(s) 683
Tsp45I GTSAC 3 cut(s) 34, 131, 140
TspDTI ATGAA 2 cut(s) 407, 611
TspGWI ACGGA 1 cut(s) 710
TspMI CCCGGG 1 cut(s) 520
Tth111I GACNNNGTC 2 cut(s) 146, 730
Vha464I CTTAAG 1 cut(s) 122
XapI RAATTY 1 cut(s) 572
XcmI CCANNNNNNNNNTGG 1 cut(s) 301
XmaI CCCGGG 1 cut(s) 520
Zsp2I ATGCAT 1 cut(s) 652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.