RLG00000021936

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000309
Physical Location & Seq
Reverse (-)
79398589 .. 79399776
1188 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021936

Sequence Viewer

Length: 849 bp
ATGGCATCCAAATCTAATCTTTCTCTCTTCACTTTCACTCTCCTCCTCGCCCTTCTTACTCTCCTCTCTTATGCAACTGCTTCTCATCGCCACAACAAGAGCTCTTCCCCATTTGAGTTCCTCGAGCATCTCAAGGGTTGTCACAATCATTTGGAGGATGATGATTTCGATGACCTTTTGGAGGAAGCCATCAAGACTTACCAGATCAATTACCACCTTAAGGCCACTGGAACATTGGATGGCAAAACAGTGTCGAAGATGATGACGCCTCGTTGTGGCCTGCCGGATATCATTAATGGCACCTCGTCCATGCGATCAGCCAAGAAAAGGCACCACCATGGTTCAATTCATACCACTGCTCACTACTCATTTCCCAATGGAAATCCAAAATGGCCTCCCTCTAAATACCATCTCACTTACGGTTTTCTCCCCGGCACCCCATCTGAAGCCACGGGTGCTGTCGCACGGGCTTTCACAACATGGGCAGGGAACACTCACTTCACGTTCAGCCAGGCCCAAAGTTACGAGAATGCGGATTTGAAGATTAGTTTTCAGAAGGGTGATCATGGAGACGGGTATCCGTTTAAGGGCTCCGGCGGAGAACTGGCCCATGCTTTTTACCCGTCAGACGGAAGATTCCACTATGACGCAGATGAGACTTGGTCTGTGGGTGCTAAACCAGGTGCTTATGACTTGGAGATGGTGGCTTTGCATGAAATAGGGCACCTTCTTGGACTAGGACATAGCTCAGTGGAGGGAGCTACCATGGAACCGCGTCTTCCCTCGGGAGTGACTAGAGGTTTGCATGGGGACGATGTTCAAGGTATCAAGGCTTTATATAACGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

283

Amino Acids

30.91

Weight (kDa)

6.45

Isoelectric Point (pI)

30.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 47 - 87 4.8e-06 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 131 - 280 3.8e-43 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 4 cut(s) 299, 330, 434, 723
AccII CGCG 1 cut(s) 775
AciI CCGC 3 cut(s) 533, 597, 773
AcuI CTGAAG 1 cut(s) 465
AcyI GRCGYC 1 cut(s) 266
AfiI CCNNNNNNNGG 6 cut(s) 181, 220, 275, 327, 587, 629
AflII CTTAAG 1 cut(s) 218
AgsI TTSAA 3 cut(s) 345, 541, 821
AjnI CCWGG 2 cut(s) 510, 679
AluBI AGCT 3 cut(s) 102, 747, 761
AluI AGCT 3 cut(s) 102, 747, 761
Alw21I GWGCWC 1 cut(s) 104
Alw26I GTCTC 2 cut(s) 564, 650
Ama87I CYCGRG 2 cut(s) 122, 784
AoxI GGCC 5 cut(s) 222, 277, 392, 513, 606
ArsI GACNNNNNNTTYG 2 cut(s) 784, 816
AseI ATTAAT 1 cut(s) 294
AspS9I GGNCC 2 cut(s) 514, 607
AsuC2I CCSGG 1 cut(s) 432
AsuHPI GGTGA 1 cut(s) 572
AvaI CYCGRG 2 cut(s) 122, 784
BaeGI GKGCMC 1 cut(s) 726
BanI GGYRCC 4 cut(s) 299, 330, 434, 723
BanII GRGCYC 2 cut(s) 104, 593
BbsI GAAGAC 1 cut(s) 770
Bbv12I GWGCWC 1 cut(s) 104
BccI CCATC 5 cut(s) 197, 233, 417, 448, 694
BciT130I CCWGG 2 cut(s) 512, 681
BciVI GTATCC 1 cut(s) 588
BclI TGATCA 1 cut(s) 562
BcnI CCSGG 1 cut(s) 432
BcoDI GTCTC 2 cut(s) 564, 650
BfaI CTAG 2 cut(s) 737, 795
BfrI CTTAAG 1 cut(s) 218
BfuI GTATCC 1 cut(s) 588
Bme1390I CCNGG 3 cut(s) 432, 512, 681
BmeT110I CYCGRG 2 cut(s) 122, 784
BmgT120I GGNCC 2 cut(s) 514, 607
BmiI GGNNCC 6 cut(s) 301, 332, 436, 592, 725, 771
BmrFI CCNGG 3 cut(s) 432, 512, 681
BmsI GCATC 2 cut(s) 14, 136
BpiI GAAGAC 1 cut(s) 770
BpuEI CTTGAG 1 cut(s) 116
BpuMI CCSGG 1 cut(s) 432
BsaHI GRCGYC 1 cut(s) 266
BsaJI CCNNGG 5 cut(s) 337, 430, 450, 765, 783
Bsc4I CCNNNNNNNGG 6 cut(s) 181, 220, 275, 327, 587, 629
Bse1I ACTGG 2 cut(s) 232, 609
BseBI CCWGG 2 cut(s) 512, 681
BseDI CCNNGG 5 cut(s) 337, 430, 450, 765, 783
BseGI GGATG 3 cut(s) 5, 163, 244
BseLI CCNNNNNNNGG 6 cut(s) 181, 220, 275, 327, 587, 629
BseMII CTCAG 1 cut(s) 762
BseNI ACTGG 2 cut(s) 232, 609
BseRI GAGGAG 3 cut(s) 32, 35, 53
BseSI GKGCMC 1 cut(s) 726
Bsh1236I CGCG 1 cut(s) 775
BshFI GGCC 5 cut(s) 224, 279, 394, 515, 608
BshNI GGYRCC 4 cut(s) 299, 330, 434, 723
BsiHKAI GWGCWC 1 cut(s) 104
BsiHKCI CYCGRG 2 cut(s) 122, 784
BsiSI CCGG 3 cut(s) 284, 432, 594
BslFI GGGAC 1 cut(s) 824
BslI CCNNNNNNNGG 6 cut(s) 181, 220, 275, 327, 587, 629
BsmAI GTCTC 2 cut(s) 564, 650
BsmBI CGTCTC 1 cut(s) 564
BsmFI GGGAC 1 cut(s) 824
BsmI GAATGC 1 cut(s) 535
BsnI GGCC 5 cut(s) 224, 279, 394, 515, 608
BsoBI CYCGRG 2 cut(s) 122, 784
Bsp1286I GDGCHC 3 cut(s) 104, 593, 726
Bsp143I GATC 3 cut(s) 204, 314, 562
Bsp19I CCATGG 2 cut(s) 337, 765
BspACI CCGC 3 cut(s) 533, 597, 773
BspANI GGCC 5 cut(s) 224, 279, 394, 515, 608
BspCNI CTCAG 1 cut(s) 761
BspFNI CGCG 1 cut(s) 775
BspLI GGNNCC 6 cut(s) 301, 332, 436, 592, 725, 771
BspQI GCTCTTC 1 cut(s) 109
BspT107I GGYRCC 4 cut(s) 299, 330, 434, 723
BspTI CTTAAG 1 cut(s) 218
BsrI ACTGG 2 cut(s) 232, 609
BssECI CCNNGG 5 cut(s) 337, 430, 450, 765, 783
BssMI GATC 3 cut(s) 204, 314, 562
BssNI GRCGYC 1 cut(s) 266
BssT1I CCWWGG 2 cut(s) 337, 765
Bst2UI CCWGG 2 cut(s) 512, 681
Bst4CI ACNGT 2 cut(s) 250, 422
Bst6I CTCTTC 2 cut(s) 32, 109
BstACI GRCGYC 1 cut(s) 266
BstAFI CTTAAG 1 cut(s) 218
BstC8I GCNNGC 1 cut(s) 281
BstDEI CTNAG 1 cut(s) 748
BstDSI CCRYGG 3 cut(s) 337, 450, 765
BstENI CCTNNNNNAGG 1 cut(s) 179
BstF5I GGATG 3 cut(s) 5, 163, 244
BstFNI CGCG 1 cut(s) 775
BstKTI GATC 3 cut(s) 207, 317, 565
BstMAI GTCTC 2 cut(s) 564, 650
BstMBI GATC 3 cut(s) 204, 314, 562
BstMWI GCNNNNNNNGC 1 cut(s) 455
BstNI CCWGG 2 cut(s) 512, 681
BstSCI CCNGG 3 cut(s) 430, 510, 679
BstSLI GKGCMC 1 cut(s) 726
BstUI CGCG 1 cut(s) 775
BstV2I GAAGAC 1 cut(s) 770
BsuI GTATCC 1 cut(s) 588
BsuRI GGCC 5 cut(s) 224, 279, 394, 515, 608
BtgI CCRYGG 3 cut(s) 337, 450, 765
BtgZI GCGATG 1 cut(s) 71
BtsCI GGATG 3 cut(s) 5, 163, 244
BtsI GCAGTG 1 cut(s) 354
BtsIMutI CAGTG 4 cut(s) 225, 255, 354, 756
Cac8I GCNNGC 1 cut(s) 281
Cfr13I GGNCC 2 cut(s) 514, 607
CseI GACGC 3 cut(s) 274, 656, 764
CsiI ACCWGGT 1 cut(s) 679
CviAII CATG 8 cut(s) 310, 338, 480, 566, 611, 713, 766, 806
DdeI CTNAG 1 cut(s) 748
DpnI GATC 3 cut(s) 206, 316, 564
DpnII GATC 3 cut(s) 204, 314, 562
Eam1104I CTCTTC 2 cut(s) 32, 109
EarI CTCTTC 2 cut(s) 32, 109
EciI GGCGGA 1 cut(s) 612
Ecl136II GAGCTC 1 cut(s) 102
Eco130I CCWWGG 2 cut(s) 337, 765
Eco24I GRGCYC 2 cut(s) 104, 593
Eco32I GATATC 1 cut(s) 289
Eco53kI GAGCTC 1 cut(s) 102
Eco57I CTGAAG 1 cut(s) 465
Eco88I CYCGRG 2 cut(s) 122, 784
EcoICRI GAGCTC 1 cut(s) 102
EcoNI CCTNNNNNAGG 1 cut(s) 179
EcoRII CCWGG 2 cut(s) 510, 679
EcoRV GATATC 1 cut(s) 289
EcoT14I CCWWGG 2 cut(s) 337, 765
EcoT38I GRGCYC 2 cut(s) 104, 593
ErhI CCWWGG 2 cut(s) 337, 765
Esp3I CGTCTC 1 cut(s) 564
FaeI CATG 8 cut(s) 313, 341, 483, 569, 614, 716, 769, 809
FaqI GGGAC 1 cut(s) 824
FatI CATG 8 cut(s) 309, 337, 479, 565, 610, 712, 765, 805
FbaI TGATCA 1 cut(s) 562
FokI GGATG 2 cut(s) 170, 251
FriOI GRGCYC 2 cut(s) 104, 593
FspBI CTAG 2 cut(s) 737, 795
HaeIII GGCC 5 cut(s) 224, 279, 394, 515, 608
HapII CCGG 3 cut(s) 284, 432, 594
HgaI GACGC 3 cut(s) 274, 656, 764
Hin1I GRCGYC 1 cut(s) 266
Hin1II CATG 8 cut(s) 313, 341, 483, 569, 614, 716, 769, 809
HinfI GANTC 1 cut(s) 636
HpaII CCGG 3 cut(s) 284, 432, 594
HphI GGTGA 1 cut(s) 572
Hpy188I TCNGA 3 cut(s) 445, 555, 628
Hpy188III TCNNGA 2 cut(s) 193, 786
HpyAV CCTTC 3 cut(s) 62, 550, 737
HpyCH4III ACNGT 2 cut(s) 250, 422
HpyCH4IV ACGT 2 cut(s) 503, 843
HpyCH4V TGCA 3 cut(s) 74, 712, 805
HpyF10VI GCNNNNNNNGC 1 cut(s) 455
HpyF3I CTNAG 1 cut(s) 748
HpySE526I ACGT 2 cut(s) 503, 843
Hsp92I GRCGYC 1 cut(s) 266
Hsp92II CATG 8 cut(s) 313, 341, 483, 569, 614, 716, 769, 809
Ksp22I TGATCA 1 cut(s) 562
Kzo9I GATC 3 cut(s) 204, 314, 562
LguI GCTCTTC 1 cut(s) 109
LmnI GCTCC 2 cut(s) 596, 758
LweI GCATC 2 cut(s) 14, 136
MabI ACCWGGT 1 cut(s) 679
MaeI CTAG 2 cut(s) 737, 795
MaeII ACGT 2 cut(s) 503, 843
MaeIII GTNAC 3 cut(s) 140, 521, 790
MalI GATC 3 cut(s) 206, 316, 564
MboI GATC 3 cut(s) 204, 314, 562
MboII GAAGA 6 cut(s) 19, 96, 268, 553, 645, 770
MhlI GDGCHC 3 cut(s) 104, 593, 726
MluCI AATT 2 cut(s) 208, 345
MseI TTAA 3 cut(s) 219, 294, 585
MslI CAYNNNNRTG 1 cut(s) 336
MspCI CTTAAG 1 cut(s) 218
MspI CCGG 3 cut(s) 284, 432, 594
MspR9I CCNGG 3 cut(s) 432, 512, 681
Mva1269I GAATGC 1 cut(s) 535
MvaI CCWGG 2 cut(s) 512, 681
MvnI CGCG 1 cut(s) 775
MwoI GCNNNNNNNGC 1 cut(s) 455
NciI CCSGG 1 cut(s) 432
NcoI CCATGG 2 cut(s) 337, 765
NdeII GATC 3 cut(s) 204, 314, 562
NlaIII CATG 8 cut(s) 313, 341, 483, 569, 614, 716, 769, 809
NlaIV GGNNCC 6 cut(s) 301, 332, 436, 592, 725, 771
NmuCI GTSAC 2 cut(s) 140, 790
PaeR7I CTCGAG 1 cut(s) 122
PciSI GCTCTTC 1 cut(s) 109
PctI GAATGC 1 cut(s) 535
PfeI GAWTC 1 cut(s) 636
PflFI GACNNNGTC 1 cut(s) 661
PshBI ATTAAT 1 cut(s) 294
Psp124BI GAGCTC 1 cut(s) 104
Psp6I CCWGG 2 cut(s) 510, 679
PspGI CCWGG 2 cut(s) 510, 679
PspN4I GGNNCC 6 cut(s) 301, 332, 436, 592, 725, 771
PspPI GGNCC 2 cut(s) 514, 607
PspXI VCTCGAGB 1 cut(s) 122
PsyI GACNNNGTC 1 cut(s) 661
RseI CAYNNNNRTG 1 cut(s) 336
SacI GAGCTC 1 cut(s) 104
SapI GCTCTTC 1 cut(s) 109
SaqAI TTAA 3 cut(s) 219, 294, 585
Sau3AI GATC 3 cut(s) 204, 314, 562
Sau96I GGNCC 2 cut(s) 514, 607
ScrFI CCNGG 3 cut(s) 432, 512, 681
SduI GDGCHC 3 cut(s) 104, 593, 726
SexAI ACCWGGT 1 cut(s) 679
SfaNI GCATC 2 cut(s) 14, 136
Sfr274I CTCGAG 1 cut(s) 122
SlaI CTCGAG 1 cut(s) 122
SmiMI CAYNNNNRTG 1 cut(s) 336
SmlI CTYRAG 3 cut(s) 122, 131, 218
SmoI CTYRAG 3 cut(s) 122, 131, 218
Sse9I AATT 2 cut(s) 208, 345
SsiI CCGC 3 cut(s) 533, 597, 773
SspMI CTAG 2 cut(s) 737, 795
SstI GAGCTC 1 cut(s) 104
StyD4I CCNGG 3 cut(s) 430, 510, 679
StyI CCWWGG 2 cut(s) 337, 765
TaaI ACNGT 2 cut(s) 250, 422
TaiI ACGT 2 cut(s) 506, 846
TaqI TCGA 3 cut(s) 123, 168, 254
TasI AATT 2 cut(s) 208, 345
TfiI GAWTC 1 cut(s) 636
Tru1I TTAA 3 cut(s) 219, 294, 585
Tru9I TTAA 3 cut(s) 219, 294, 585
TscAI CASTG 4 cut(s) 232, 255, 361, 756
TseFI GTSAC 2 cut(s) 140, 790
Tsp45I GTSAC 2 cut(s) 140, 790
TspDTI ATGAA 2 cut(s) 338, 729
TspGWI ACGGA 2 cut(s) 570, 645
TspRI CASTG 4 cut(s) 232, 255, 361, 756
Tth111I GACNNNGTC 1 cut(s) 661
Vha464I CTTAAG 1 cut(s) 218
VspI ATTAAT 1 cut(s) 294
XagI CCTNNNNNAGG 1 cut(s) 179
XcmI CCANNNNNNNNNTGG 1 cut(s) 232
XhoI CTCGAG 1 cut(s) 122
XspI CTAG 2 cut(s) 737, 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.