pycom17g03730

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0000309
Physical Location & Seq
Forward (+)
2538279 .. 2539208
930 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g03730.2

Sequence Viewer

Length: 846 bp
ATGGCTTCAAAAAGTAATCTCGCTCTTTTCACAATCACTCTCCTCGTCGTCCTCCTTTCTCTGTTCTCCGAAGCAACGTCGTCAGGCACCCACAACAAAAAAAAATCACCATTCGAGTTTCTCAACCATCTCAAGGGGAGTCACAAGGGTGACAAGGTCCAAGGCATCCAAGATCTAAAAAAATACCTTGAAAAATTCGGATATTTAAATGGCCGTTCCGATAATGATGACTTCGACGACATATTGGAGTCGGCAATCAAAACCTACCAAGTCAATTACCACCTCAAAGCCACCGGGACATTAGATAAAAGGCAACCTCATCACCATGACGGACATGGTTCGACTCACACGGTTGCCCATTTTTCTTTCTTCTCCGGAAACCCTATATGGCCTTCATCTAAGTACCATCTCACCTACGCTTTTGATCAATGCACCCCAGCTGAGGCTCAGGCCGCAGTGGCGCGAGCTTTTGCAACATGGGCAGGAAACACACACTTCTCGTTCAGTCAAGCCCAAAGTTACGAGAACGCAGATCTGAAGATCAGTTTTGGTCGGAGTGATCATGGAGATGGGAATCCTTTTGATGGCCGCAGTGGGACTCTTGCTCATGCATTTGCACCGACCAATGGGAGATTCCACTATGATGCCGATGAGCCGTGGGCTGTGGGAGCTGTGGAGGGTGCTTATGACTTGGAAACCGTGGCTTTGCATGAAATCGGGCATCTGCTTGGGCTAGGACATAGCTCTGTTGAAGGAGCTGTCATGTCTTCCGGTGTTCGGACTGGATTTACCCAAAGTTTGCATGCGGATGATATTCAAGGAATTAAAGCTTTATATAGCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

282

Amino Acids

30.68

Weight (kDa)

6.23

Isoelectric Point (pI)

28.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 86
AccII CGCG 1 cut(s) 463
AccIII TCCGGA 1 cut(s) 374
AciI CCGC 3 cut(s) 453, 589, 806
AcoI YGGCCR 2 cut(s) 211, 586
AcsI RAATTY 1 cut(s) 194
AcuI CTGAAG 1 cut(s) 557
AfaI GTAC 1 cut(s) 404
AfiI CCNNNNNNNGG 5 cut(s) 133, 442, 584, 626, 777
AgsI TTSAA 4 cut(s) 9, 191, 752, 818
AjuI GAANNNNNNNTTGG 2 cut(s) 617, 649
AleI CACNNNNGTG 1 cut(s) 147
AluBI AGCT 6 cut(s) 440, 467, 671, 744, 758, 830
AluI AGCT 6 cut(s) 440, 467, 671, 744, 758, 830
Aor13HI TCCGGA 1 cut(s) 374
AoxI GGCC 4 cut(s) 211, 389, 450, 586
ApoI RAATTY 1 cut(s) 194
AspLEI GCGC 1 cut(s) 463
AspS9I GGNCC 1 cut(s) 157
AsuC2I CCSGG 1 cut(s) 295
AsuHPI GGTGA 4 cut(s) 99, 161, 314, 403
AvaII GGWCC 1 cut(s) 157
BanI GGYRCC 1 cut(s) 86
BbsI GAAGAC 1 cut(s) 759
BbvCI CCTCAGC 1 cut(s) 441
BccI CCATC 4 cut(s) 135, 414, 563, 578
BceAI ACGGC 2 cut(s) 198, 640
BclI TGATCA 2 cut(s) 424, 559
BcnI CCSGG 1 cut(s) 295
BfaI CTAG 1 cut(s) 734
BglI GCCNNNNNGGC 1 cut(s) 458
BglII AGATCT 2 cut(s) 172, 532
BisI GCNGC 2 cut(s) 453, 589
BlsI GCNGC 2 cut(s) 454, 590
Bme1390I CCNGG 1 cut(s) 295
Bme18I GGWCC 1 cut(s) 157
BmgT120I GGNCC 1 cut(s) 157
BmiI GGNNCC 1 cut(s) 88
BmrFI CCNGG 1 cut(s) 295
BmsI GCATC 3 cut(s) 174, 634, 730
BpiI GAAGAC 1 cut(s) 759
Bpu10I CCTNAGC 2 cut(s) 441, 447
BpuEI CTTGAG 1 cut(s) 116
BpuMI CCSGG 1 cut(s) 295
BsaBI GATNNNNATC 1 cut(s) 573
BsaJI CCNNGG 3 cut(s) 160, 656, 699
BsaWI WCCGGW 2 cut(s) 374, 770
Bsc4I CCNNNNNNNGG 5 cut(s) 133, 442, 584, 626, 777
Bse1I ACTGG 1 cut(s) 787
Bse8I GATNNNNATC 1 cut(s) 573
BseAI TCCGGA 1 cut(s) 374
BseDI CCNNGG 3 cut(s) 160, 656, 699
BseGI GGATG 2 cut(s) 165, 814
BseJI GATNNNNATC 1 cut(s) 573
BseLI CCNNNNNNNGG 5 cut(s) 133, 442, 584, 626, 777
BseMII CTCAG 2 cut(s) 432, 461
BseNI ACTGG 1 cut(s) 787
BseRI GAGGAG 1 cut(s) 32
BseYI CCCAGC 1 cut(s) 436
Bsh1236I CGCG 1 cut(s) 463
BshFI GGCC 4 cut(s) 213, 391, 452, 588
BshNI GGYRCC 1 cut(s) 86
BsiSI CCGG 3 cut(s) 294, 375, 771
BslFI GGGAC 2 cut(s) 310, 610
BslI CCNNNNNNNGG 5 cut(s) 133, 442, 584, 626, 777
BsmFI GGGAC 2 cut(s) 310, 610
BsnI GGCC 4 cut(s) 213, 391, 452, 588
Bsp13I TCCGGA 1 cut(s) 374
Bsp143I GATC 5 cut(s) 172, 424, 532, 540, 559
BspACI CCGC 3 cut(s) 453, 589, 806
BspANI GGCC 4 cut(s) 213, 391, 452, 588
BspCNI CTCAG 2 cut(s) 433, 460
BspEI TCCGGA 1 cut(s) 374
BspFNI CGCG 1 cut(s) 463
BspLI GGNNCC 1 cut(s) 88
BspT107I GGYRCC 1 cut(s) 86
BsrI ACTGG 1 cut(s) 787
BssECI CCNNGG 3 cut(s) 160, 656, 699
BssMI GATC 5 cut(s) 172, 424, 532, 540, 559
BssT1I CCWWGG 1 cut(s) 160
Bst4CI ACNGT 2 cut(s) 352, 700
BstC8I GCNNGC 2 cut(s) 465, 804
BstDEI CTNAG 3 cut(s) 399, 441, 447
BstDSI CCRYGG 2 cut(s) 656, 699
BstF5I GGATG 2 cut(s) 165, 814
BstFNI CGCG 1 cut(s) 463
BstHHI GCGC 1 cut(s) 463
BstKTI GATC 5 cut(s) 175, 427, 535, 543, 562
BstMBI GATC 5 cut(s) 172, 424, 532, 540, 559
BstMWI GCNNNNNNNGC 4 cut(s) 452, 458, 479, 668
BstNSI RCATGY 1 cut(s) 806
BstSCI CCNGG 1 cut(s) 293
BstUI CGCG 1 cut(s) 463
BstV2I GAAGAC 1 cut(s) 759
BstX2I RGATCY 2 cut(s) 172, 532
BstYI RGATCY 2 cut(s) 172, 532
BsuRI GGCC 4 cut(s) 213, 391, 452, 588
BtgI CCRYGG 2 cut(s) 656, 699
BtsCI GGATG 2 cut(s) 165, 814
BtsI GCAGTG 2 cut(s) 462, 598
BtsIMutI CAGTG 2 cut(s) 462, 598
Cac8I GCNNGC 2 cut(s) 465, 804
CfoI GCGC 1 cut(s) 463
Cfr13I GGNCC 1 cut(s) 157
Csp6I GTAC 1 cut(s) 403
CviAII CATG 8 cut(s) 326, 335, 477, 563, 608, 710, 763, 803
CviQI GTAC 1 cut(s) 403
DdeI CTNAG 3 cut(s) 399, 441, 447
DpnI GATC 5 cut(s) 174, 426, 534, 542, 561
DpnII GATC 5 cut(s) 172, 424, 532, 540, 559
DraI TTTAAA 1 cut(s) 207
EaeI YGGCCR 2 cut(s) 211, 586
Eco130I CCWWGG 1 cut(s) 160
Eco47I GGWCC 1 cut(s) 157
Eco57I CTGAAG 1 cut(s) 557
EcoT14I CCWWGG 1 cut(s) 160
EcoT22I ATGCAT 1 cut(s) 613
ErhI CCWWGG 1 cut(s) 160
FaeI CATG 8 cut(s) 329, 338, 480, 566, 611, 713, 766, 806
FaqI GGGAC 2 cut(s) 310, 610
FatI CATG 8 cut(s) 325, 334, 476, 562, 607, 709, 762, 802
FbaI TGATCA 2 cut(s) 424, 559
Fnu4HI GCNGC 2 cut(s) 453, 589
FokI GGATG 2 cut(s) 152, 821
Fsp4HI GCNGC 2 cut(s) 453, 589
FspBI CTAG 1 cut(s) 734
GlaI GCGC 1 cut(s) 462
GluI GCNGC 2 cut(s) 453, 589
GsaI CCCAGC 1 cut(s) 440
HaeIII GGCC 4 cut(s) 213, 391, 452, 588
HapII CCGG 3 cut(s) 294, 375, 771
HhaI GCGC 1 cut(s) 463
Hin1II CATG 8 cut(s) 329, 338, 480, 566, 611, 713, 766, 806
Hin6I GCGC 1 cut(s) 461
HinP1I GCGC 1 cut(s) 461
HindIII AAGCTT 1 cut(s) 828
HinfI GANTC 6 cut(s) 139, 248, 343, 574, 598, 633
HpaII CCGG 3 cut(s) 294, 375, 771
HphI GGTGA 4 cut(s) 99, 161, 314, 403
Hpy188I TCNGA 6 cut(s) 70, 200, 220, 537, 555, 780
Hpy188III TCNNGA 1 cut(s) 375
Hpy99I CGWCG 3 cut(s) 50, 82, 239
HpyAV CCTTC 2 cut(s) 402, 746
HpyCH4III ACNGT 2 cut(s) 352, 700
HpyCH4IV ACGT 1 cut(s) 77
HpyCH4V TGCA 6 cut(s) 432, 473, 611, 617, 709, 802
HpyF10VI GCNNNNNNNGC 4 cut(s) 452, 458, 479, 668
HpyF3I CTNAG 3 cut(s) 399, 441, 447
HpySE526I ACGT 1 cut(s) 77
Hsp92II CATG 8 cut(s) 329, 338, 480, 566, 611, 713, 766, 806
HspAI GCGC 1 cut(s) 461
Kpn2I TCCGGA 1 cut(s) 374
Ksp22I TGATCA 2 cut(s) 424, 559
Kzo9I GATC 5 cut(s) 172, 424, 532, 540, 559
LmnI GCTCC 2 cut(s) 668, 755
LpnPI CCDG 8 cut(s) 69, 307, 388, 434, 450, 468, 768, 784
LweI GCATC 3 cut(s) 174, 634, 730
MaeI CTAG 1 cut(s) 734
MaeII ACGT 1 cut(s) 77
MaeIII GTNAC 3 cut(s) 140, 149, 518
MalI GATC 5 cut(s) 174, 426, 534, 542, 561
MboI GATC 5 cut(s) 172, 424, 532, 540, 559
MboII GAAGA 3 cut(s) 361, 550, 759
MflI RGATCY 2 cut(s) 172, 532
MluCI AATT 3 cut(s) 194, 274, 822
MlyI GAGTC 4 cut(s) 148, 257, 337, 592
MmeI TCCRAC 1 cut(s) 533
MnlI CCTC 6 cut(s) 53, 62, 293, 327, 436, 670
Mph1103I ATGCAT 1 cut(s) 613
MroI TCCGGA 1 cut(s) 374
MseI TTAA 2 cut(s) 206, 825
MslI CAYNNNNRTG 5 cut(s) 147, 324, 567, 642, 807
MspA1I CMGCKG 1 cut(s) 440
MspI CCGG 3 cut(s) 294, 375, 771
MspR9I CCNGG 1 cut(s) 295
MvnI CGCG 1 cut(s) 463
MwoI GCNNNNNNNGC 4 cut(s) 452, 458, 479, 668
NciI CCSGG 1 cut(s) 295
NdeII GATC 5 cut(s) 172, 424, 532, 540, 559
NlaIII CATG 8 cut(s) 329, 338, 480, 566, 611, 713, 766, 806
NlaIV GGNNCC 1 cut(s) 88
NmuCI GTSAC 2 cut(s) 140, 149
NsiI ATGCAT 1 cut(s) 613
NspI RCATGY 1 cut(s) 806
OliI CACNNNNGTG 1 cut(s) 147
PaeI GCATGC 1 cut(s) 806
PfeI GAWTC 2 cut(s) 574, 633
PflFI GACNNNGTC 1 cut(s) 155
PkrI GCNGC 2 cut(s) 454, 590
PleI GAGTC 4 cut(s) 147, 256, 337, 592
PpsI GAGTC 4 cut(s) 147, 256, 337, 592
PspFI CCCAGC 1 cut(s) 436
PspN4I GGNNCC 1 cut(s) 88
PspPI GGNCC 1 cut(s) 157
PsuI RGATCY 2 cut(s) 172, 532
PsyI GACNNNGTC 1 cut(s) 155
PvuII CAGCTG 1 cut(s) 440
RsaI GTAC 1 cut(s) 404
RsaNI GTAC 1 cut(s) 403
RseI CAYNNNNRTG 5 cut(s) 147, 324, 567, 642, 807
SaqAI TTAA 2 cut(s) 206, 825
SatI GCNGC 2 cut(s) 453, 589
Sau3AI GATC 5 cut(s) 172, 424, 532, 540, 559
Sau96I GGNCC 1 cut(s) 157
SchI GAGTC 4 cut(s) 148, 257, 337, 592
ScrFI CCNGG 1 cut(s) 295
SfaNI GCATC 3 cut(s) 174, 634, 730
SinI GGWCC 1 cut(s) 157
SmiI ATTTAAAT 1 cut(s) 207
SmiMI CAYNNNNRTG 5 cut(s) 147, 324, 567, 642, 807
SmlI CTYRAG 1 cut(s) 131
SmoI CTYRAG 1 cut(s) 131
SphI GCATGC 1 cut(s) 806
Sse9I AATT 3 cut(s) 194, 274, 822
SsiI CCGC 3 cut(s) 453, 589, 806
SspMI CTAG 1 cut(s) 734
StyD4I CCNGG 1 cut(s) 293
StyI CCWWGG 1 cut(s) 160
SwaI ATTTAAAT 1 cut(s) 207
TaaI ACNGT 2 cut(s) 352, 700
TaiI ACGT 1 cut(s) 80
TaqI TCGA 3 cut(s) 114, 234, 341
TasI AATT 3 cut(s) 194, 274, 822
TauI GCSGC 2 cut(s) 455, 591
TfiI GAWTC 2 cut(s) 574, 633
Tru1I TTAA 2 cut(s) 206, 825
Tru9I TTAA 2 cut(s) 206, 825
TscAI CASTG 2 cut(s) 462, 598
TseFI GTSAC 2 cut(s) 140, 149
Tsp45I GTSAC 2 cut(s) 140, 149
TspDTI ATGAA 2 cut(s) 384, 726
TspGWI ACGGA 1 cut(s) 345
TspRI CASTG 2 cut(s) 462, 598
Tth111I GACNNNGTC 1 cut(s) 155
VpaK11BI GGWCC 1 cut(s) 157
XapI RAATTY 1 cut(s) 194
XceI RCATGY 1 cut(s) 806
XcmI CCANNNNNNNNNTGG 1 cut(s) 332
XspI CTAG 1 cut(s) 734
Zsp2I ATGCAT 1 cut(s) 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.