RLG00000021932

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000309
Physical Location & Seq
Reverse (-)
79380654 .. 79381871
1218 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021932

Sequence Viewer

Length: 888 bp
ATGGCATCCAAATCTAATCTTTCTCTCTTCACTGTCACTCTCCTCCTTGCCCTTCTCACTCTCCTCTCTTATGCAACTGCTTCTCATCCCCACAACAAGAGCTCTTCCCCATTTGAGTTCCTTGAGCATCTCAAGGGTTGCCACAAAGGTGATAAGGTCAAGGGCATCAATGATCTCAAAAAATACCTTGAAAAGTACGGTTACTTGAACTACAAGAACCACAGTCATTCGGACGACGATGATTTTGATGACCTTTTGGAGGAAGCCATCAAGACTTACCAGGTCAATTACCACCTCAAGTCCACCGGAACACTGGACGGCAAAACAGTGTCGAAGATGATGACACCTCGTTGTGGCATGCCGGATATCATCAACGGCACCTCGTCCATGAGATCAGCAAGAAAAGGCACCACCATGGGAAATCCAAAATGGCCTTCTTCTAAATATCATCTCACTTACGGTTTTCTCCCCAGCACCCCATCCGAAGCCACGGGCGCTGTCGCACGGGGTTTCGCAACATGGGCTGGAAACACTCACTTCACGTTCAGCCAGTCCCAAAGTTACGAGAATGCGGATTTGAAGATTAGTTTTCATAAGGGTGATCATGGAGACGGGAATTCATTTGATGGCCCGGGCGGGGTCCTAGCCCATGCATTTAGCCCGTCGGATGGAAGATTCCACTACGACGCAGATGAGCCTTGGTCTGTGGGTGCTACACCAGGTGCTTATGACTTGGAGACTGTGGCTTTGCATGAAATAGGGCACCTTCTAGGACTTGGACATAGCTCAGTGGAGGGAGCTATCATGGCCCCAGCTATTCCCCCGGGAGTGACTAAAAGTTTGCATGGGGACGATGTTCAAGGAATCAAGGCTTTATACAACGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

296

Amino Acids

31.95

Weight (kDa)

6.36

Isoelectric Point (pI)

28.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 56 - 113 2.5e-10 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 143 - 293 3.7e-45 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 377, 407, 762
AciI CCGC 2 cut(s) 572, 636
AcsI RAATTY 1 cut(s) 616
AdeI CACNNNGTG 1 cut(s) 722
AfaI GTAC 1 cut(s) 197
AfiI CCNNNNNNNGG 4 cut(s) 259, 353, 637, 668
AgsI TTSAA 4 cut(s) 191, 208, 580, 860
AjnI CCWGG 2 cut(s) 279, 718
AjuI GAANNNNNNNTTGG 2 cut(s) 418, 450
AleI CACNNNNGTG 1 cut(s) 147
AluBI AGCT 4 cut(s) 102, 786, 800, 815
AluI AGCT 4 cut(s) 102, 786, 800, 815
Alw21I GWGCWC 1 cut(s) 104
Alw26I GTCTC 2 cut(s) 603, 731
Ama87I CYCGRG 2 cut(s) 631, 823
AoxI GGCC 3 cut(s) 431, 628, 807
ApoI RAATTY 1 cut(s) 616
ArsI GACNNNNNNTTYG 6 cut(s) 227, 259, 730, 762, 823, 855
AspLEI GCGC 1 cut(s) 497
AspS9I GGNCC 3 cut(s) 629, 640, 808
AsuC2I CCSGG 4 cut(s) 632, 633, 824, 825
AsuHPI GGTGA 2 cut(s) 161, 611
AvaI CYCGRG 2 cut(s) 631, 823
AvaII GGWCC 1 cut(s) 640
BaeGI GKGCMC 1 cut(s) 765
BanI GGYRCC 3 cut(s) 377, 407, 762
BanII GRGCYC 1 cut(s) 104
Bbv12I GWGCWC 1 cut(s) 104
BccI CCATC 4 cut(s) 275, 487, 620, 662
BceAI ACGGC 2 cut(s) 334, 391
BciT130I CCWGG 2 cut(s) 281, 720
BclI TGATCA 1 cut(s) 601
BcnI CCSGG 4 cut(s) 632, 633, 824, 825
BcoDI GTCTC 2 cut(s) 603, 731
BfaI CTAG 2 cut(s) 644, 770
BfoI RGCGCY 1 cut(s) 498
Bme1390I CCNGG 6 cut(s) 281, 632, 633, 720, 824, 825
Bme18I GGWCC 1 cut(s) 640
BmeT110I CYCGRG 2 cut(s) 631, 823
BmgT120I GGNCC 3 cut(s) 629, 640, 808
BmiI GGNNCC 5 cut(s) 379, 409, 641, 764, 810
BmrFI CCNGG 6 cut(s) 281, 632, 633, 720, 824, 825
BmsI GCATC 3 cut(s) 14, 136, 174
BpuEI CTTGAG 3 cut(s) 116, 143, 281
BpuMI CCSGG 4 cut(s) 632, 633, 824, 825
BsaJI CCNNGG 6 cut(s) 414, 489, 631, 698, 822, 823
BsaWI WCCGGW 1 cut(s) 305
Bsc4I CCNNNNNNNGG 4 cut(s) 259, 353, 637, 668
Bse1I ACTGG 2 cut(s) 318, 550
BseBI CCWGG 2 cut(s) 281, 720
BseDI CCNNGG 6 cut(s) 414, 489, 631, 698, 822, 823
BseGI GGATG 4 cut(s) 5, 85, 479, 673
BseLI CCNNNNNNNGG 4 cut(s) 259, 353, 637, 668
BseMII CTCAG 1 cut(s) 801
BseNI ACTGG 2 cut(s) 318, 550
BseRI GAGGAG 2 cut(s) 32, 53
BseSI GKGCMC 1 cut(s) 765
BseYI CCCAGC 2 cut(s) 470, 811
BshFI GGCC 3 cut(s) 433, 630, 809
BshNI GGYRCC 3 cut(s) 377, 407, 762
BsiHKAI GWGCWC 1 cut(s) 104
BsiHKCI CYCGRG 2 cut(s) 631, 823
BsiSI CCGG 4 cut(s) 306, 362, 632, 824
BslFI GGGAC 2 cut(s) 538, 863
BslI CCNNNNNNNGG 4 cut(s) 259, 353, 637, 668
BsmAI GTCTC 2 cut(s) 603, 731
BsmBI CGTCTC 1 cut(s) 603
BsmFI GGGAC 2 cut(s) 538, 863
BsmI GAATGC 1 cut(s) 574
BsnI GGCC 3 cut(s) 433, 630, 809
BsoBI CYCGRG 2 cut(s) 631, 823
Bsp1286I GDGCHC 2 cut(s) 104, 765
Bsp143I GATC 3 cut(s) 172, 392, 601
Bsp19I CCATGG 1 cut(s) 414
BspACI CCGC 2 cut(s) 572, 636
BspANI GGCC 3 cut(s) 433, 630, 809
BspCNI CTCAG 1 cut(s) 800
BspLI GGNNCC 5 cut(s) 379, 409, 641, 764, 810
BspQI GCTCTTC 1 cut(s) 109
BspT107I GGYRCC 3 cut(s) 377, 407, 762
BsrI ACTGG 2 cut(s) 318, 550
BssECI CCNNGG 6 cut(s) 414, 489, 631, 698, 822, 823
BssMI GATC 3 cut(s) 172, 392, 601
BssT1I CCWWGG 2 cut(s) 414, 698
Bst2UI CCWGG 2 cut(s) 281, 720
Bst4CI ACNGT 6 cut(s) 34, 200, 224, 328, 461, 742
Bst6I CTCTTC 2 cut(s) 32, 109
BstC8I GCNNGC 1 cut(s) 359
BstDEI CTNAG 1 cut(s) 787
BstDSI CCRYGG 2 cut(s) 414, 489
BstENI CCTNNNNNAGG 1 cut(s) 257
BstF5I GGATG 4 cut(s) 5, 85, 479, 673
BstH2I RGCGCY 1 cut(s) 498
BstHHI GCGC 1 cut(s) 497
BstKTI GATC 3 cut(s) 175, 395, 604
BstMAI GTCTC 2 cut(s) 603, 731
BstMBI GATC 3 cut(s) 172, 392, 601
BstMWI GCNNNNNNNGC 3 cut(s) 494, 521, 806
BstNI CCWGG 2 cut(s) 281, 720
BstNSI RCATGY 1 cut(s) 361
BstSCI CCNGG 6 cut(s) 279, 630, 631, 718, 822, 823
BstSLI GKGCMC 1 cut(s) 765
BsuRI GGCC 3 cut(s) 433, 630, 809
BtgI CCRYGG 2 cut(s) 414, 489
BtsCI GGATG 4 cut(s) 5, 85, 479, 673
BtsIMutI CAGTG 4 cut(s) 30, 311, 333, 795
Cac8I GCNNGC 1 cut(s) 359
CfoI GCGC 1 cut(s) 497
Cfr13I GGNCC 3 cut(s) 629, 640, 808
Cfr9I CCCGGG 2 cut(s) 631, 823
CseI GACGC 1 cut(s) 695
CsiI ACCWGGT 2 cut(s) 279, 718
Csp6I GTAC 1 cut(s) 196
CviAII CATG 9 cut(s) 358, 388, 415, 519, 605, 650, 752, 805, 845
CviQI GTAC 1 cut(s) 196
DdeI CTNAG 1 cut(s) 787
DpnI GATC 3 cut(s) 174, 394, 603
DpnII GATC 3 cut(s) 172, 392, 601
DraIII CACNNNGTG 1 cut(s) 722
Eam1104I CTCTTC 2 cut(s) 32, 109
EarI CTCTTC 2 cut(s) 32, 109
Ecl136II GAGCTC 1 cut(s) 102
Eco130I CCWWGG 2 cut(s) 414, 698
Eco24I GRGCYC 1 cut(s) 104
Eco32I GATATC 1 cut(s) 367
Eco47I GGWCC 1 cut(s) 640
Eco53kI GAGCTC 1 cut(s) 102
Eco88I CYCGRG 2 cut(s) 631, 823
EcoICRI GAGCTC 1 cut(s) 102
EcoNI CCTNNNNNAGG 1 cut(s) 257
EcoO109I RGGNCCY 1 cut(s) 640
EcoRI GAATTC 1 cut(s) 616
EcoRII CCWGG 2 cut(s) 279, 718
EcoRV GATATC 1 cut(s) 367
EcoT14I CCWWGG 2 cut(s) 414, 698
EcoT22I ATGCAT 1 cut(s) 655
EcoT38I GRGCYC 1 cut(s) 104
ErhI CCWWGG 2 cut(s) 414, 698
Esp3I CGTCTC 1 cut(s) 603
FaeI CATG 9 cut(s) 361, 391, 418, 522, 608, 653, 755, 808, 848
FaqI GGGAC 2 cut(s) 538, 863
FatI CATG 9 cut(s) 357, 387, 414, 518, 604, 649, 751, 804, 844
FauI CCCGC 1 cut(s) 629
FbaI TGATCA 1 cut(s) 601
FokI GGATG 3 cut(s) 72, 466, 680
FriOI GRGCYC 1 cut(s) 104
FspBI CTAG 2 cut(s) 644, 770
GlaI GCGC 1 cut(s) 496
GsaI CCCAGC 2 cut(s) 474, 815
HaeII RGCGCY 1 cut(s) 498
HaeIII GGCC 3 cut(s) 433, 630, 809
HapII CCGG 4 cut(s) 306, 362, 632, 824
HgaI GACGC 1 cut(s) 695
HhaI GCGC 1 cut(s) 497
Hin1II CATG 9 cut(s) 361, 391, 418, 522, 608, 653, 755, 808, 848
Hin6I GCGC 1 cut(s) 495
HinP1I GCGC 1 cut(s) 495
HinfI GANTC 2 cut(s) 675, 864
HpaII CCGG 4 cut(s) 306, 362, 632, 824
HphI GGTGA 2 cut(s) 161, 611
Hpy166II GTNNAC 1 cut(s) 303
Hpy188I TCNGA 4 cut(s) 232, 484, 667, 887
Hpy188III TCNNGA 1 cut(s) 271
Hpy8I GTNNAC 1 cut(s) 303
Hpy99I CGWCG 3 cut(s) 239, 667, 689
HpyAV CCTTC 3 cut(s) 62, 444, 776
HpyCH4III ACNGT 6 cut(s) 34, 200, 224, 328, 461, 742
HpyCH4IV ACGT 2 cut(s) 542, 882
HpyCH4V TGCA 4 cut(s) 74, 653, 751, 844
HpyF10VI GCNNNNNNNGC 3 cut(s) 494, 521, 806
HpyF3I CTNAG 1 cut(s) 787
HpySE526I ACGT 2 cut(s) 542, 882
Hsp92II CATG 9 cut(s) 361, 391, 418, 522, 608, 653, 755, 808, 848
HspAI GCGC 1 cut(s) 495
Ksp22I TGATCA 1 cut(s) 601
Kzo9I GATC 3 cut(s) 172, 392, 601
LguI GCTCTTC 1 cut(s) 109
LmnI GCTCC 1 cut(s) 797
LweI GCATC 3 cut(s) 14, 136, 174
MabI ACCWGGT 2 cut(s) 279, 718
MaeI CTAG 2 cut(s) 644, 770
MaeII ACGT 2 cut(s) 542, 882
MaeIII GTNAC 4 cut(s) 34, 200, 560, 829
MalI GATC 3 cut(s) 174, 394, 603
MboI GATC 3 cut(s) 172, 392, 601
MboII GAAGA 6 cut(s) 19, 96, 346, 429, 592, 684
MhlI GDGCHC 2 cut(s) 104, 765
MluCI AATT 2 cut(s) 286, 616
MmeI TCCRAC 1 cut(s) 645
MnlI CCTC 7 cut(s) 53, 74, 253, 305, 357, 391, 787
Mph1103I ATGCAT 1 cut(s) 655
MslI CAYNNNNRTG 3 cut(s) 147, 413, 597
MspI CCGG 4 cut(s) 306, 362, 632, 824
MspR9I CCNGG 6 cut(s) 281, 632, 633, 720, 824, 825
Mva1269I GAATGC 1 cut(s) 574
MvaI CCWGG 2 cut(s) 281, 720
MwoI GCNNNNNNNGC 3 cut(s) 494, 521, 806
NciI CCSGG 4 cut(s) 632, 633, 824, 825
NcoI CCATGG 1 cut(s) 414
NdeII GATC 3 cut(s) 172, 392, 601
NlaIII CATG 9 cut(s) 361, 391, 418, 522, 608, 653, 755, 808, 848
NlaIV GGNNCC 5 cut(s) 379, 409, 641, 764, 810
NmuCI GTSAC 2 cut(s) 34, 829
NsiI ATGCAT 1 cut(s) 655
NspI RCATGY 1 cut(s) 361
OliI CACNNNNGTG 1 cut(s) 147
PaeI GCATGC 1 cut(s) 361
PciSI GCTCTTC 1 cut(s) 109
PctI GAATGC 1 cut(s) 574
PfeI GAWTC 2 cut(s) 675, 864
PpuMI RGGWCCY 1 cut(s) 640
Psp124BI GAGCTC 1 cut(s) 104
Psp5II RGGWCCY 1 cut(s) 640
Psp6I CCWGG 2 cut(s) 279, 718
PspFI CCCAGC 2 cut(s) 470, 811
PspGI CCWGG 2 cut(s) 279, 718
PspN4I GGNNCC 5 cut(s) 379, 409, 641, 764, 810
PspPI GGNCC 3 cut(s) 629, 640, 808
PspPPI RGGWCCY 1 cut(s) 640
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
RseI CAYNNNNRTG 3 cut(s) 147, 413, 597
SacI GAGCTC 1 cut(s) 104
SapI GCTCTTC 1 cut(s) 109
Sau3AI GATC 3 cut(s) 172, 392, 601
Sau96I GGNCC 3 cut(s) 629, 640, 808
ScrFI CCNGG 6 cut(s) 281, 632, 633, 720, 824, 825
SduI GDGCHC 2 cut(s) 104, 765
SexAI ACCWGGT 2 cut(s) 279, 718
SfaNI GCATC 3 cut(s) 14, 136, 174
SinI GGWCC 1 cut(s) 640
SmaI CCCGGG 2 cut(s) 633, 825
SmiMI CAYNNNNRTG 3 cut(s) 147, 413, 597
SmlI CTYRAG 3 cut(s) 122, 131, 296
SmoI CTYRAG 3 cut(s) 122, 131, 296
SphI GCATGC 1 cut(s) 361
SrfI GCCCGGGC 1 cut(s) 633
Sse9I AATT 2 cut(s) 286, 616
SsiI CCGC 2 cut(s) 572, 636
SspMI CTAG 2 cut(s) 644, 770
SstI GAGCTC 1 cut(s) 104
StyD4I CCNGG 6 cut(s) 279, 630, 631, 718, 822, 823
StyI CCWWGG 2 cut(s) 414, 698
TaaI ACNGT 6 cut(s) 34, 200, 224, 328, 461, 742
TaiI ACGT 2 cut(s) 545, 885
TaqI TCGA 1 cut(s) 332
TasI AATT 2 cut(s) 286, 616
TfiI GAWTC 2 cut(s) 675, 864
TscAI CASTG 4 cut(s) 37, 318, 333, 795
TseFI GTSAC 2 cut(s) 34, 829
Tsp45I GTSAC 2 cut(s) 34, 829
TspDTI ATGAA 3 cut(s) 581, 609, 768
TspMI CCCGGG 2 cut(s) 631, 823
TspRI CASTG 4 cut(s) 37, 318, 333, 795
VpaK11BI GGWCC 1 cut(s) 640
XagI CCTNNNNNAGG 1 cut(s) 257
XapI RAATTY 1 cut(s) 616
XceI RCATGY 1 cut(s) 361
XcmI CCANNNNNNNNNTGG 1 cut(s) 310
XmaI CCCGGG 2 cut(s) 631, 823
XspI CTAG 2 cut(s) 644, 770
Zsp2I ATGCAT 1 cut(s) 655
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.