Rroxscaffold_3G00236660

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000309
Physical Location & Seq
Reverse (-)
24007456 .. 24008392
937 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00236660.1

Sequence Viewer

Length: 576 bp
ATGGCATCCAAATCTAATCTTTCTCTCTTCACTATCACTCTCCTCCTTGCCCTTCTCACTCTCCTCTCTTCTGCAACTGCTTCTCATCCCCACAACAAGAGCTCTTCCCCATTTGAGTTCCTTGAGCATCTCAAGGGTTGCCACAAAGGTGATAAGGTCAAGGGCATCAATGACCTCAAGAAATACCTTGAAAAGTACGGTTACTTGAAATACAAGAATCACAGTCATTCGGACGATGATGATTTTGACGACCTTTTGGAGGAAGCCATCAAGACTTACCAGATCAATTACCACCTCAAGTCCACCGGAACATTGGATGGCAAAACAGTGTCGAAGATGATGACGCCTCGTTGTGGCGTGCCGGATATCATCAATGGCACCTCGTCCATGAGATCAGCCAAGAAAAGGCACCACCATGCCACGGGCGCTGTCGCACGGGCTTTCGCAACATGGGCTGGGAACACTCACTTCACGTTTAGCCAGTCACAAAGTTACGAGAGTACGGATTTGAAGATTAGTTTTCATAAGGGTGATCATGGAGACGGAATCCATTTGATGGCCCCGGCGGGATCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

191

Amino Acids

21.1

Weight (kDa)

8.94

Isoelectric Point (pI)

29.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 56 - 113 2.6e-10 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 135 - 184 8.9e-07 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 377, 408
AccB7I CCANNNNNTGG 1 cut(s) 556
AciI CCGC 1 cut(s) 566
AclWI GGATC 1 cut(s) 564
AcyI GRCGYC 1 cut(s) 344
AfaI GTAC 2 cut(s) 197, 502
AfiI CCNNNNNNNGG 5 cut(s) 259, 353, 405, 421, 556
AgsI TTSAA 3 cut(s) 191, 208, 511
AleI CACNNNNGTG 1 cut(s) 147
AluBI AGCT 1 cut(s) 102
AluI AGCT 1 cut(s) 102
Alw21I GWGCWC 1 cut(s) 104
Alw26I GTCTC 1 cut(s) 534
AlwI GGATC 1 cut(s) 564
AoxI GGCC 1 cut(s) 558
AspLEI GCGC 1 cut(s) 428
AspS9I GGNCC 1 cut(s) 559
AsuC2I CCSGG 1 cut(s) 563
AsuHPI GGTGA 2 cut(s) 161, 542
BamHI GGATCC 1 cut(s) 569
BanI GGYRCC 2 cut(s) 377, 408
BanII GRGCYC 1 cut(s) 104
Bbv12I GWGCWC 1 cut(s) 104
BccI CCATC 3 cut(s) 275, 311, 550
BclI TGATCA 1 cut(s) 532
BcnI CCSGG 1 cut(s) 563
BcoDI GTCTC 1 cut(s) 534
BfaI CTAG 1 cut(s) 574
BfoI RGCGCY 1 cut(s) 429
Bme1390I CCNGG 1 cut(s) 563
BmgT120I GGNCC 1 cut(s) 559
BmiI GGNNCC 4 cut(s) 379, 410, 561, 571
BmrFI CCNGG 1 cut(s) 563
BmsI GCATC 3 cut(s) 14, 136, 174
BpuEI CTTGAG 4 cut(s) 116, 143, 161, 281
BpuMI CCSGG 1 cut(s) 563
BsaHI GRCGYC 1 cut(s) 344
BsaJI CCNNGG 2 cut(s) 420, 561
BsaWI WCCGGW 1 cut(s) 305
Bsc4I CCNNNNNNNGG 5 cut(s) 259, 353, 405, 421, 556
Bse1I ACTGG 1 cut(s) 481
BseDI CCNNGG 2 cut(s) 420, 561
BseGI GGATG 3 cut(s) 5, 85, 322
BseLI CCNNNNNNNGG 5 cut(s) 259, 353, 405, 421, 556
BseNI ACTGG 1 cut(s) 481
BseRI GAGGAG 2 cut(s) 32, 53
BseYI CCCAGC 1 cut(s) 455
BshFI GGCC 1 cut(s) 560
BshNI GGYRCC 2 cut(s) 377, 408
BsiHKAI GWGCWC 1 cut(s) 104
BsiSI CCGG 3 cut(s) 306, 362, 563
BslI CCNNNNNNNGG 5 cut(s) 259, 353, 405, 421, 556
BsmAI GTCTC 1 cut(s) 534
BsmBI CGTCTC 1 cut(s) 534
BsnI GGCC 1 cut(s) 560
Bsp1286I GDGCHC 1 cut(s) 104
Bsp143I GATC 4 cut(s) 282, 392, 532, 569
BspACI CCGC 1 cut(s) 566
BspANI GGCC 1 cut(s) 560
BspLI GGNNCC 4 cut(s) 379, 410, 561, 571
BspPI GGATC 1 cut(s) 564
BspQI GCTCTTC 1 cut(s) 109
BspT107I GGYRCC 2 cut(s) 377, 408
BsrI ACTGG 1 cut(s) 481
BssECI CCNNGG 2 cut(s) 420, 561
BssMI GATC 4 cut(s) 282, 392, 532, 569
BssNI GRCGYC 1 cut(s) 344
Bst4CI ACNGT 3 cut(s) 200, 224, 328
Bst6I CTCTTC 3 cut(s) 32, 73, 109
BstACI GRCGYC 1 cut(s) 344
BstC8I GCNNGC 1 cut(s) 359
BstDSI CCRYGG 1 cut(s) 420
BstENI CCTNNNNNAGG 1 cut(s) 257
BstF5I GGATG 3 cut(s) 5, 85, 322
BstH2I RGCGCY 1 cut(s) 429
BstHHI GCGC 1 cut(s) 428
BstKTI GATC 4 cut(s) 285, 395, 535, 572
BstMAI GTCTC 1 cut(s) 534
BstMBI GATC 4 cut(s) 282, 392, 532, 569
BstMWI GCNNNNNNNGC 2 cut(s) 425, 452
BstSCI CCNGG 1 cut(s) 561
BstX2I RGATCY 1 cut(s) 569
BstYI RGATCY 1 cut(s) 569
BsuRI GGCC 1 cut(s) 560
BtgI CCRYGG 1 cut(s) 420
BtsCI GGATG 3 cut(s) 5, 85, 322
BtsIMutI CAGTG 1 cut(s) 333
Cac8I GCNNGC 1 cut(s) 359
CfoI GCGC 1 cut(s) 428
Cfr13I GGNCC 1 cut(s) 559
CseI GACGC 1 cut(s) 352
Csp6I GTAC 2 cut(s) 196, 501
CviAII CATG 4 cut(s) 388, 416, 450, 536
CviJI RGCY 7 cut(s) 102, 266, 398, 440, 455, 480, 560
CviKI_1 RGCY 7 cut(s) 102, 266, 398, 440, 455, 480, 560
CviQI GTAC 2 cut(s) 196, 501
DpnI GATC 4 cut(s) 284, 394, 534, 571
DpnII GATC 4 cut(s) 282, 392, 532, 569
Eam1104I CTCTTC 3 cut(s) 32, 73, 109
EarI CTCTTC 3 cut(s) 32, 73, 109
Ecl136II GAGCTC 1 cut(s) 102
Eco24I GRGCYC 1 cut(s) 104
Eco32I GATATC 1 cut(s) 367
Eco53kI GAGCTC 1 cut(s) 102
EcoICRI GAGCTC 1 cut(s) 102
EcoNI CCTNNNNNAGG 1 cut(s) 257
EcoRV GATATC 1 cut(s) 367
EcoT38I GRGCYC 1 cut(s) 104
Esp3I CGTCTC 1 cut(s) 534
FaeI CATG 4 cut(s) 391, 419, 453, 539
FaiI YATR 5 cut(s) 389, 417, 451, 525, 537
FatI CATG 4 cut(s) 387, 415, 449, 535
FauI CCCGC 1 cut(s) 559
FbaI TGATCA 1 cut(s) 532
FokI GGATG 2 cut(s) 72, 329
FriOI GRGCYC 1 cut(s) 104
FspBI CTAG 1 cut(s) 574
GlaI GCGC 1 cut(s) 427
GsaI CCCAGC 1 cut(s) 459
HaeII RGCGCY 1 cut(s) 429
HaeIII GGCC 1 cut(s) 560
HapII CCGG 3 cut(s) 306, 362, 563
HgaI GACGC 1 cut(s) 352
HhaI GCGC 1 cut(s) 428
Hin1I GRCGYC 1 cut(s) 344
Hin1II CATG 4 cut(s) 391, 419, 453, 539
Hin6I GCGC 1 cut(s) 426
HinP1I GCGC 1 cut(s) 426
HinfI GANTC 2 cut(s) 217, 546
HpaII CCGG 3 cut(s) 306, 362, 563
HphI GGTGA 2 cut(s) 161, 542
Hpy166II GTNNAC 1 cut(s) 303
Hpy188I TCNGA 1 cut(s) 232
Hpy188III TCNNGA 2 cut(s) 178, 271
Hpy8I GTNNAC 1 cut(s) 303
HpyAV CCTTC 1 cut(s) 62
HpyCH4III ACNGT 3 cut(s) 200, 224, 328
HpyCH4IV ACGT 1 cut(s) 473
HpyCH4V TGCA 1 cut(s) 74
HpyF10VI GCNNNNNNNGC 2 cut(s) 425, 452
HpySE526I ACGT 1 cut(s) 473
Hsp92I GRCGYC 1 cut(s) 344
Hsp92II CATG 4 cut(s) 391, 419, 453, 539
HspAI GCGC 1 cut(s) 426
Ksp22I TGATCA 1 cut(s) 532
Kzo9I GATC 4 cut(s) 282, 392, 532, 569
LguI GCTCTTC 1 cut(s) 109
LpnPI CCDG 5 cut(s) 293, 319, 375, 441, 494
LweI GCATC 3 cut(s) 14, 136, 174
MaeI CTAG 1 cut(s) 574
MaeII ACGT 1 cut(s) 473
MaeIII GTNAC 3 cut(s) 200, 483, 491
MalI GATC 4 cut(s) 284, 394, 534, 571
MboI GATC 4 cut(s) 282, 392, 532, 569
MboII GAAGA 5 cut(s) 19, 60, 96, 346, 523
MflI RGATCY 1 cut(s) 569
MhlI GDGCHC 1 cut(s) 104
MluCI AATT 1 cut(s) 286
MnlI CCTC 7 cut(s) 53, 74, 185, 253, 305, 357, 391
MslI CAYNNNNRTG 3 cut(s) 147, 414, 528
MspI CCGG 3 cut(s) 306, 362, 563
MspR9I CCNGG 1 cut(s) 563
MwoI GCNNNNNNNGC 2 cut(s) 425, 452
NciI CCSGG 1 cut(s) 563
NdeII GATC 4 cut(s) 282, 392, 532, 569
NlaIII CATG 4 cut(s) 391, 419, 453, 539
NlaIV GGNNCC 4 cut(s) 379, 410, 561, 571
NmuCI GTSAC 1 cut(s) 483
OliI CACNNNNGTG 1 cut(s) 147
PciSI GCTCTTC 1 cut(s) 109
PfeI GAWTC 2 cut(s) 217, 546
PflMI CCANNNNNTGG 1 cut(s) 556
Psp124BI GAGCTC 1 cut(s) 104
PspFI CCCAGC 1 cut(s) 455
PspN4I GGNNCC 4 cut(s) 379, 410, 561, 571
PspPI GGNCC 1 cut(s) 559
PsuI RGATCY 1 cut(s) 569
RsaI GTAC 2 cut(s) 197, 502
RsaNI GTAC 2 cut(s) 196, 501
RseI CAYNNNNRTG 3 cut(s) 147, 414, 528
SacI GAGCTC 1 cut(s) 104
SapI GCTCTTC 1 cut(s) 109
Sau3AI GATC 4 cut(s) 282, 392, 532, 569
Sau96I GGNCC 1 cut(s) 559
ScrFI CCNGG 1 cut(s) 563
SduI GDGCHC 1 cut(s) 104
SetI ASST 9 cut(s) 104, 151, 159, 177, 189, 255, 297, 383, 476
SfaNI GCATC 3 cut(s) 14, 136, 174
SmiMI CAYNNNNRTG 3 cut(s) 147, 414, 528
SmlI CTYRAG 4 cut(s) 122, 131, 176, 296
SmoI CTYRAG 4 cut(s) 122, 131, 176, 296
Sse9I AATT 1 cut(s) 286
SsiI CCGC 1 cut(s) 566
SspMI CTAG 1 cut(s) 574
SstI GAGCTC 1 cut(s) 104
StyD4I CCNGG 1 cut(s) 561
TaaI ACNGT 3 cut(s) 200, 224, 328
TaiI ACGT 1 cut(s) 476
TaqI TCGA 1 cut(s) 332
TasI AATT 1 cut(s) 286
TfiI GAWTC 2 cut(s) 217, 546
TscAI CASTG 1 cut(s) 333
TseFI GTSAC 1 cut(s) 483
Tsp45I GTSAC 1 cut(s) 483
TspDTI ATGAA 1 cut(s) 512
TspGWI ACGGA 2 cut(s) 518, 558
TspRI CASTG 1 cut(s) 333
Van91I CCANNNNNTGG 1 cut(s) 556
XagI CCTNNNNNAGG 1 cut(s) 257
XcmI CCANNNNNNNNNTGG 1 cut(s) 310
XspI CTAG 1 cut(s) 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.