Rh2AG618400

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000309
Physical Location & Seq
Reverse (-)
84240032 .. 84241452
1421 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG618400.1

Sequence Viewer

Length: 918 bp
ATGGCACCAAAATCTGATCTTTCTCTTTTCAAAGTCACTCCCCTCCTCCTCCTCGCCCTCTTTTCTTTCCTCTCTAATGCAGCGGCAAACTCATCTCCGCTTGAGTTCCTTGAGCATCTTAAGGGTTGTCAAAAAGGTGACAAGGTCAAGGGCATCAATGACCTCAAGAAGTACCTTCACAACTTCGGTTACTTGAACTACAAGAACCACATTCATTCTGATGACGATGATTTCGACGAGCTCTTGGAGGAAGCCGTCAAGACTTACCAGCTCAATTTCCACCTCAAGTCCACCGGAACGTTGGACGACAAAACCATATTACAGATGATGATGCCTCGTTGTGGCGTGCCCGATATCGTCAATGGCACCACATCCATGCGAGCAGCCAAGATAAAGCACCAGCACGGTTCAATTCATACCACTGTTCACTACTCATTTCCTAATGGAAAACCAAAATGGCCTTCCTCTAAATACCATCTGACATACGCTTTCTTCCCCGGCACCCCATCCCAAGCCACGGGTGCTATAGCACAGGCTTTCGCAACATGGGCTAGAAGCACTCACTTCAAGTTCAGTCAGGCCCAAAATTACCAGAATGCAGATGTCAAGATTAGTTTTCACCGAGGTAATCATGGAGACGGGCGTAATTTTGATGGGCCTGGCGGAGTGCTGGCCCATGCTTTTTACCCTACGGATGGAAGATTCCACTTCGACGCAGCTGAAAATTGGTCCGCGGGTGCTAAACCAGGTGCCTATGACTTGGAGAGTGTTGCTTTGCACGAAATAGGGCATCTTCTGGGACTTGGACATAGCTCAGTGAAGGGAGCTGTCATGGCAGCAACCATCTCCCCGGGAGTCACTCTTAAAAGTTTGAACGGGGATGATATTCAAGGAATTAAAGCTTTATACAACGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

305

Amino Acids

33.42

Weight (kDa)

8.35

Isoelectric Point (pI)

18.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 52 - 107 5e-10 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 152 - 303 3.6e-47 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 4 cut(s) 4, 365, 500, 749
AccII CGCG 1 cut(s) 734
AciI CCGC 5 cut(s) 83, 98, 663, 732, 734
AclI AACGTT 2 cut(s) 299, 912
AfaI GTAC 1 cut(s) 173
AfiI CCNNNNNNNGG 3 cut(s) 341, 517, 695
AflII CTTAAG 1 cut(s) 119
AgsI TTSAA 6 cut(s) 31, 196, 411, 568, 874, 890
AjnI CCWGG 2 cut(s) 658, 745
AjuI GAANNNNNNNTTGG 2 cut(s) 445, 477
AluBI AGCT 6 cut(s) 241, 271, 719, 813, 827, 902
AluI AGCT 6 cut(s) 241, 271, 719, 813, 827, 902
Alw21I GWGCWC 1 cut(s) 243
Alw26I GTCTC 1 cut(s) 630
Ama87I CYCGRG 1 cut(s) 850
AoxI GGCC 4 cut(s) 458, 579, 656, 672
ApeKI GCWGC 4 cut(s) 80, 383, 716, 836
ArsI GACNNNNNNTTYG 2 cut(s) 215, 247
AspS9I GGNCC 4 cut(s) 580, 656, 673, 729
AsuC2I CCSGG 3 cut(s) 498, 851, 852
AsuHPI GGTGA 2 cut(s) 149, 611
AvaI CYCGRG 1 cut(s) 850
AvaII GGWCC 1 cut(s) 729
BaeGI GKGCMC 1 cut(s) 351
BanI GGYRCC 4 cut(s) 4, 365, 500, 749
BanII GRGCYC 1 cut(s) 243
Bbv12I GWGCWC 1 cut(s) 243
BbvI GCAGC 4 cut(s) 92, 395, 728, 848
BccI CCATC 5 cut(s) 483, 514, 647, 689, 851
BceAI ACGGC 1 cut(s) 239
BciT130I CCWGG 2 cut(s) 660, 747
BcnI CCSGG 3 cut(s) 498, 851, 852
BcoDI GTCTC 1 cut(s) 630
BfaI CTAG 1 cut(s) 552
BfmI CTRYAG 1 cut(s) 525
BfrI CTTAAG 1 cut(s) 119
BisI GCNGC 5 cut(s) 81, 84, 384, 717, 837
BlsI GCNGC 5 cut(s) 82, 85, 385, 718, 838
Bme1390I CCNGG 5 cut(s) 498, 660, 747, 851, 852
Bme18I GGWCC 1 cut(s) 729
BmeT110I CYCGRG 1 cut(s) 850
BmgT120I GGNCC 4 cut(s) 580, 656, 673, 729
BmiI GGNNCC 4 cut(s) 6, 367, 502, 751
BmrFI CCNGG 5 cut(s) 498, 660, 747, 851, 852
BmsI GCATC 4 cut(s) 124, 162, 321, 799
BpuEI CTTGAG 4 cut(s) 122, 131, 149, 269
BpuMI CCSGG 3 cut(s) 498, 851, 852
BsaJI CCNNGG 6 cut(s) 496, 516, 622, 732, 849, 850
BsaWI WCCGGW 1 cut(s) 293
Bsc4I CCNNNNNNNGG 3 cut(s) 341, 517, 695
BseBI CCWGG 2 cut(s) 660, 747
BseDI CCNNGG 6 cut(s) 496, 516, 622, 732, 849, 850
BseGI GGATG 4 cut(s) 371, 506, 700, 886
BseLI CCNNNNNNNGG 3 cut(s) 341, 517, 695
BseMII CTCAG 1 cut(s) 828
BseRI GAGGAG 3 cut(s) 35, 38, 41
BseSI GKGCMC 1 cut(s) 351
BseXI GCAGC 4 cut(s) 92, 395, 728, 848
Bsh1236I CGCG 1 cut(s) 734
BshFI GGCC 4 cut(s) 460, 581, 658, 674
BshNI GGYRCC 4 cut(s) 4, 365, 500, 749
BsiHKAI GWGCWC 1 cut(s) 243
BsiHKCI CYCGRG 1 cut(s) 850
BsiSI CCGG 3 cut(s) 294, 498, 851
BslFI GGGAC 1 cut(s) 813
BslI CCNNNNNNNGG 3 cut(s) 341, 517, 695
BsmAI GTCTC 1 cut(s) 630
BsmBI CGTCTC 1 cut(s) 630
BsmFI GGGAC 1 cut(s) 813
BsmI GAATGC 1 cut(s) 601
BsnI GGCC 4 cut(s) 460, 581, 658, 674
BsoBI CYCGRG 1 cut(s) 850
Bsp1286I GDGCHC 2 cut(s) 243, 351
Bsp143I GATC 1 cut(s) 16
BspACI CCGC 5 cut(s) 83, 98, 663, 732, 734
BspANI GGCC 4 cut(s) 460, 581, 658, 674
BspCNI CTCAG 1 cut(s) 827
BspFNI CGCG 1 cut(s) 734
BspLI GGNNCC 4 cut(s) 6, 367, 502, 751
BspT107I GGYRCC 4 cut(s) 4, 365, 500, 749
BspTI CTTAAG 1 cut(s) 119
BssECI CCNNGG 6 cut(s) 496, 516, 622, 732, 849, 850
BssMI GATC 1 cut(s) 16
Bst2UI CCWGG 2 cut(s) 660, 747
Bst4CI ACNGT 2 cut(s) 407, 424
BstAFI CTTAAG 1 cut(s) 119
BstC8I GCNNGC 3 cut(s) 347, 381, 672
BstDEI CTNAG 1 cut(s) 814
BstDSI CCRYGG 2 cut(s) 516, 732
BstF5I GGATG 4 cut(s) 371, 506, 700, 886
BstFNI CGCG 1 cut(s) 734
BstKTI GATC 1 cut(s) 19
BstMAI GTCTC 1 cut(s) 630
BstMBI GATC 1 cut(s) 16
BstMWI GCNNNNNNNGC 3 cut(s) 521, 548, 833
BstNI CCWGG 2 cut(s) 660, 747
BstSCI CCNGG 5 cut(s) 496, 658, 745, 849, 850
BstSFI CTRYAG 1 cut(s) 525
BstSLI GKGCMC 1 cut(s) 351
BstUI CGCG 1 cut(s) 734
BstV1I GCAGC 4 cut(s) 92, 395, 728, 848
BsuRI GGCC 4 cut(s) 460, 581, 658, 674
BtgI CCRYGG 2 cut(s) 516, 732
BtsCI GGATG 4 cut(s) 371, 506, 700, 886
BtsIMutI CAGTG 2 cut(s) 420, 822
Cac8I GCNNGC 3 cut(s) 347, 381, 672
Cfr13I GGNCC 4 cut(s) 580, 656, 673, 729
Cfr42I CCGCGG 1 cut(s) 735
Cfr9I CCCGGG 1 cut(s) 850
CseI GACGC 1 cut(s) 722
CsiI ACCWGGT 1 cut(s) 745
Csp6I GTAC 1 cut(s) 172
CviAII CATG 5 cut(s) 376, 546, 632, 677, 832
CviQI GTAC 1 cut(s) 172
DdeI CTNAG 1 cut(s) 814
DpnI GATC 1 cut(s) 18
DpnII GATC 1 cut(s) 16
EciI GGCGGA 1 cut(s) 678
Ecl136II GAGCTC 1 cut(s) 241
Eco24I GRGCYC 1 cut(s) 243
Eco32I GATATC 1 cut(s) 355
Eco47I GGWCC 1 cut(s) 729
Eco53kI GAGCTC 1 cut(s) 241
Eco88I CYCGRG 1 cut(s) 850
EcoICRI GAGCTC 1 cut(s) 241
EcoRII CCWGG 2 cut(s) 658, 745
EcoRV GATATC 1 cut(s) 355
EcoT38I GRGCYC 1 cut(s) 243
Esp3I CGTCTC 1 cut(s) 630
FaeI CATG 5 cut(s) 379, 549, 635, 680, 835
FaqI GGGAC 1 cut(s) 813
FatI CATG 5 cut(s) 375, 545, 631, 676, 831
FauI CCCGC 1 cut(s) 727
Fnu4HI GCNGC 5 cut(s) 81, 84, 384, 717, 837
FokI GGATG 4 cut(s) 358, 493, 707, 893
FriOI GRGCYC 1 cut(s) 243
Fsp4HI GCNGC 5 cut(s) 81, 84, 384, 717, 837
FspBI CTAG 1 cut(s) 552
GluI GCNGC 5 cut(s) 81, 84, 384, 717, 837
HaeIII GGCC 4 cut(s) 460, 581, 658, 674
HapII CCGG 3 cut(s) 294, 498, 851
HgaI GACGC 1 cut(s) 722
Hin1II CATG 5 cut(s) 379, 549, 635, 680, 835
HindIII AAGCTT 1 cut(s) 900
HinfI GANTC 2 cut(s) 702, 855
HpaII CCGG 3 cut(s) 294, 498, 851
HphI GGTGA 2 cut(s) 149, 611
Hpy166II GTNNAC 2 cut(s) 291, 427
Hpy188I TCNGA 3 cut(s) 16, 220, 480
Hpy188III TCNNGA 3 cut(s) 166, 259, 607
Hpy8I GTNNAC 2 cut(s) 291, 427
Hpy99I CGWCG 2 cut(s) 239, 716
HpyAV CCTTC 3 cut(s) 185, 471, 814
HpyCH4III ACNGT 2 cut(s) 407, 424
HpyCH4IV ACGT 2 cut(s) 299, 912
HpyCH4V TGCA 3 cut(s) 80, 599, 778
HpyF10VI GCNNNNNNNGC 3 cut(s) 521, 548, 833
HpyF3I CTNAG 1 cut(s) 814
HpySE526I ACGT 2 cut(s) 299, 912
Hsp92II CATG 5 cut(s) 379, 549, 635, 680, 835
KspI CCGCGG 1 cut(s) 735
Kzo9I GATC 1 cut(s) 16
LmnI GCTCC 1 cut(s) 824
Lsp1109I GCAGC 4 cut(s) 92, 395, 728, 848
LweI GCATC 4 cut(s) 124, 162, 321, 799
MabI ACCWGGT 1 cut(s) 745
MaeI CTAG 1 cut(s) 552
MaeII ACGT 2 cut(s) 299, 912
MaeIII GTNAC 4 cut(s) 34, 137, 188, 856
MalI GATC 1 cut(s) 18
MboI GATC 1 cut(s) 16
MboII GAAGA 3 cut(s) 484, 711, 785
MhlI GDGCHC 2 cut(s) 243, 351
MluCI AATT 6 cut(s) 274, 411, 586, 646, 724, 894
MlyI GAGTC 1 cut(s) 864
MmeI TCCRAC 1 cut(s) 282
MseI TTAA 3 cut(s) 120, 864, 897
MslI CAYNNNNRTG 2 cut(s) 219, 374
MspA1I CMGCKG 3 cut(s) 83, 719, 734
MspCI CTTAAG 1 cut(s) 119
MspI CCGG 3 cut(s) 294, 498, 851
MspR9I CCNGG 5 cut(s) 498, 660, 747, 851, 852
Mva1269I GAATGC 1 cut(s) 601
MvaI CCWGG 2 cut(s) 660, 747
MvnI CGCG 1 cut(s) 734
MwoI GCNNNNNNNGC 3 cut(s) 521, 548, 833
NciI CCSGG 3 cut(s) 498, 851, 852
NdeII GATC 1 cut(s) 16
NlaIII CATG 5 cut(s) 379, 549, 635, 680, 835
NlaIV GGNNCC 4 cut(s) 6, 367, 502, 751
NmuCI GTSAC 3 cut(s) 34, 137, 856
PcsI WCGNNNNNNNCGW 1 cut(s) 231
PctI GAATGC 1 cut(s) 601
PfeI GAWTC 1 cut(s) 702
PflFI GACNNNGTC 1 cut(s) 143
PkrI GCNGC 5 cut(s) 82, 85, 385, 718, 838
PleI GAGTC 1 cut(s) 863
PpsI GAGTC 1 cut(s) 863
Psp124BI GAGCTC 1 cut(s) 243
Psp1406I AACGTT 2 cut(s) 299, 912
Psp6I CCWGG 2 cut(s) 658, 745
PspGI CCWGG 2 cut(s) 658, 745
PspN4I GGNNCC 4 cut(s) 6, 367, 502, 751
PspPI GGNCC 4 cut(s) 580, 656, 673, 729
PsyI GACNNNGTC 1 cut(s) 143
PvuII CAGCTG 1 cut(s) 719
RsaI GTAC 1 cut(s) 173
RsaNI GTAC 1 cut(s) 172
RseI CAYNNNNRTG 2 cut(s) 219, 374
SacI GAGCTC 1 cut(s) 243
SacII CCGCGG 1 cut(s) 735
SaqAI TTAA 3 cut(s) 120, 864, 897
SatI GCNGC 5 cut(s) 81, 84, 384, 717, 837
Sau3AI GATC 1 cut(s) 16
Sau96I GGNCC 4 cut(s) 580, 656, 673, 729
SchI GAGTC 1 cut(s) 864
ScrFI CCNGG 5 cut(s) 498, 660, 747, 851, 852
SduI GDGCHC 2 cut(s) 243, 351
SexAI ACCWGGT 1 cut(s) 745
SfaNI GCATC 4 cut(s) 124, 162, 321, 799
SfcI CTRYAG 1 cut(s) 525
Sfr303I CCGCGG 1 cut(s) 735
SgrBI CCGCGG 1 cut(s) 735
SinI GGWCC 1 cut(s) 729
SmaI CCCGGG 1 cut(s) 852
SmiMI CAYNNNNRTG 2 cut(s) 219, 374
SmlI CTYRAG 5 cut(s) 101, 110, 119, 164, 284
SmoI CTYRAG 5 cut(s) 101, 110, 119, 164, 284
Sse9I AATT 6 cut(s) 274, 411, 586, 646, 724, 894
SsiI CCGC 5 cut(s) 83, 98, 663, 732, 734
SspMI CTAG 1 cut(s) 552
SstI GAGCTC 1 cut(s) 243
StyD4I CCNGG 5 cut(s) 496, 658, 745, 849, 850
TaaI ACNGT 2 cut(s) 407, 424
TaiI ACGT 2 cut(s) 302, 915
TaqI TCGA 2 cut(s) 234, 711
TasI AATT 6 cut(s) 274, 411, 586, 646, 724, 894
TauI GCSGC 1 cut(s) 86
TfiI GAWTC 1 cut(s) 702
Tru1I TTAA 3 cut(s) 120, 864, 897
Tru9I TTAA 3 cut(s) 120, 864, 897
TscAI CASTG 2 cut(s) 427, 822
TseFI GTSAC 3 cut(s) 34, 137, 856
TseI GCWGC 4 cut(s) 80, 383, 716, 836
Tsp45I GTSAC 3 cut(s) 34, 137, 856
TspDTI ATGAA 2 cut(s) 203, 404
TspGWI ACGGA 1 cut(s) 707
TspMI CCCGGG 1 cut(s) 850
TspRI CASTG 2 cut(s) 427, 822
Tth111I GACNNNGTC 1 cut(s) 143
Vha464I CTTAAG 1 cut(s) 119
VpaK11BI GGWCC 1 cut(s) 729
XcmI CCANNNNNNNNNTGG 1 cut(s) 298
XmaI CCCGGG 1 cut(s) 850
XspI CTAG 1 cut(s) 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.