Prupe.3G278000_v2.0.a1

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0000309
Physical Location & Seq
Reverse (-)
25518624 .. 25519556
933 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G278000.1

Sequence Viewer

Length: 933 bp
ATGGCAATGGCATCATCAAAAGCTACAACTCTTTCTCTCTTCACAATCACTACCCTCCTCTTCATCCTCCTTTCTCTCCTCTCCTGTGCAAAAGCATCAGAAACCCACAACAAAACATCATCACCATTTGAGTTCCTTGACCATCTCAAGGGGTGTCACAAGGGTGACAAAGTTCAAGGCATCCAAGACCTCAAAAAATACCTCGAAAGGTTTGGTTACTTGAATGGCGATATCAACAACGATGACTTTGACGACCAGTTGGAGGCGGCCATCAAAACCTACCAAATCAATTACCATCTCAAGGCCACGGGAACATTGGATACAAAAACCGTATCGAAAATGATGATGCCTCGATGTGGTGTGCCAGATATCATCAATGGCACCACCGCTATGCGATCAGGCAAAAAAAGGCACCCTCACCACCACGGGGGGCACACAGTTGCTCATTATGCTTTCTTCCAAGGAAGCGCAAAATGGCCTGCCAATAAGTATCACCTCACCTATGGTTTTGCTCAAGGCACCCCGGCTAATGCCGTGGGCGCAGTTACACGTGCTTTTGCAACATGGGCAGGCAACACGCACTTCTCTTTCAGTCGGGCTAAAAGCGTTGACAGCGCTGATCTGAAGATCAGTTTTGGAAGTGGTGAACATGGAGATGGACAACCGTTTGATGGGCCAGGTGGGGTCCTAGCTCATGCTTATGCGCCAACAAATGGGAGATTTCACTACGATGCTGATGAGACGTGGGTTGTGGGTGCTGTGCCTGGGGGAATGGATTTGGAGACTGTGGCTTTGCATGAAATTGGTCATCTTCTAGGGCTTGCGCATAGCTCTGTTGAAGGAGCTGTCATGTTACCTGGAATCCGCGCTGGATTTACCCAGAGTTTGCATGCGGATGATATTCAAGGAATTAAAGCTTTATACAACACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

311

Amino Acids

33.45

Weight (kDa)

7.03

Isoelectric Point (pI)

25.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 825
AccB1I GGYRCC 3 cut(s) 380, 411, 518
AccB7I CCANNNNNTGG 1 cut(s) 713
AccII CGCG 1 cut(s) 867
AciI CCGC 4 cut(s) 266, 387, 865, 893
AcoI YGGCCR 1 cut(s) 267
AcuI CTGAAG 1 cut(s) 644
AcvI CACGTG 1 cut(s) 551
AfeI AGCGCT 1 cut(s) 616
AfiI CCNNNNNNNGG 7 cut(s) 148, 262, 301, 356, 427, 671, 713
AflIII ACRYGT 1 cut(s) 548
AgsI TTSAA 4 cut(s) 176, 223, 839, 905
AjiI CACGTC 1 cut(s) 744
AjnI CCWGG 3 cut(s) 676, 763, 856
AleI CACNNNNGTG 1 cut(s) 162
AluBI AGCT 5 cut(s) 23, 692, 831, 845, 917
AluI AGCT 5 cut(s) 23, 692, 831, 845, 917
Alw26I GTCTC 2 cut(s) 734, 776
Aor51HI AGCGCT 1 cut(s) 616
AoxI GGCC 4 cut(s) 267, 303, 476, 674
ArsI GACNNNNNNTTYG 2 cut(s) 775, 807
AspLEI GCGC 6 cut(s) 470, 542, 617, 706, 826, 869
AspS9I GGNCC 2 cut(s) 674, 685
AsuC2I CCSGG 1 cut(s) 524
AsuHPI GGTGA 6 cut(s) 114, 176, 410, 485, 490, 656
AvaII GGWCC 1 cut(s) 685
BaeGI GKGCMC 1 cut(s) 435
BanI GGYRCC 3 cut(s) 380, 411, 518
BbrPI CACGTG 1 cut(s) 551
BccI CCATC 5 cut(s) 150, 278, 303, 650, 665
BceAI ACGGC 1 cut(s) 518
BciT130I CCWGG 3 cut(s) 678, 765, 858
BciVI GTATCC 1 cut(s) 313
BcnI CCSGG 1 cut(s) 524
BcoDI GTCTC 2 cut(s) 734, 776
BfaI CTAG 2 cut(s) 689, 815
BfoI RGCGCY 1 cut(s) 618
BfuI GTATCC 1 cut(s) 313
BisI GCNGC 1 cut(s) 267
BlsI GCNGC 1 cut(s) 268
Bme1390I CCNGG 4 cut(s) 524, 678, 765, 858
Bme18I GGWCC 1 cut(s) 685
BmgBI CACGTC 1 cut(s) 744
BmgT120I GGNCC 2 cut(s) 674, 685
BmiI GGNNCC 4 cut(s) 382, 413, 520, 686
BmrFI CCNGG 4 cut(s) 524, 678, 765, 858
BmsI GCATC 5 cut(s) 20, 104, 189, 336, 721
BpuEI CTTGAG 3 cut(s) 131, 284, 498
BpuMI CCSGG 1 cut(s) 524
BsaAI YACGTR 1 cut(s) 551
BsaJI CCNNGG 6 cut(s) 306, 424, 460, 522, 534, 764
Bsc4I CCNNNNNNNGG 7 cut(s) 148, 262, 301, 356, 427, 671, 713
Bse1I ACTGG 1 cut(s) 256
Bse3DI GCAATG 1 cut(s) 12
BseBI CCWGG 3 cut(s) 678, 765, 858
BseDI CCNNGG 6 cut(s) 306, 424, 460, 522, 534, 764
BseGI GGATG 3 cut(s) 63, 180, 901
BseLI CCNNNNNNNGG 7 cut(s) 148, 262, 301, 356, 427, 671, 713
BseMI GCAATG 1 cut(s) 12
BseNI ACTGG 1 cut(s) 256
BseRI GAGGAG 2 cut(s) 47, 68
BseSI GKGCMC 1 cut(s) 435
Bsh1236I CGCG 1 cut(s) 867
BshFI GGCC 4 cut(s) 269, 305, 478, 676
BshNI GGYRCC 3 cut(s) 380, 411, 518
BsiSI CCGG 1 cut(s) 524
BslI CCNNNNNNNGG 7 cut(s) 148, 262, 301, 356, 427, 671, 713
BsmAI GTCTC 2 cut(s) 734, 776
BsmBI CGTCTC 1 cut(s) 734
BsnI GGCC 4 cut(s) 269, 305, 478, 676
Bsp1286I GDGCHC 1 cut(s) 435
Bsp143I GATC 3 cut(s) 395, 619, 627
BspACI CCGC 4 cut(s) 266, 387, 865, 893
BspANI GGCC 4 cut(s) 269, 305, 478, 676
BspFNI CGCG 1 cut(s) 867
BspLI GGNNCC 4 cut(s) 382, 413, 520, 686
BspT107I GGYRCC 3 cut(s) 380, 411, 518
BsrDI GCAATG 1 cut(s) 12
BsrI ACTGG 1 cut(s) 256
BssECI CCNNGG 6 cut(s) 306, 424, 460, 522, 534, 764
BssMI GATC 3 cut(s) 395, 619, 627
BssT1I CCWWGG 1 cut(s) 460
Bst2UI CCWGG 3 cut(s) 678, 765, 858
Bst4CI ACNGT 4 cut(s) 331, 439, 666, 787
Bst6I CTCTTC 2 cut(s) 44, 65
BstBAI YACGTR 1 cut(s) 551
BstC8I GCNNGC 4 cut(s) 480, 571, 822, 891
BstDSI CCRYGG 3 cut(s) 306, 424, 534
BstF5I GGATG 3 cut(s) 63, 180, 901
BstFNI CGCG 1 cut(s) 867
BstH2I RGCGCY 1 cut(s) 618
BstHHI GCGC 6 cut(s) 470, 542, 617, 706, 826, 869
BstKTI GATC 3 cut(s) 398, 622, 630
BstMAI GTCTC 2 cut(s) 734, 776
BstMBI GATC 3 cut(s) 395, 619, 627
BstMWI GCNNNNNNNGC 4 cut(s) 449, 539, 566, 612
BstNI CCWGG 3 cut(s) 678, 765, 858
BstNSI RCATGY 1 cut(s) 893
BstSCI CCNGG 4 cut(s) 522, 676, 763, 856
BstSLI GKGCMC 1 cut(s) 435
BstUI CGCG 1 cut(s) 867
BsuI GTATCC 1 cut(s) 313
BsuRI GGCC 4 cut(s) 269, 305, 478, 676
BtgI CCRYGG 3 cut(s) 306, 424, 534
BtrI CACGTC 1 cut(s) 744
BtsCI GGATG 3 cut(s) 63, 180, 901
Cac8I GCNNGC 4 cut(s) 480, 571, 822, 891
CfoI GCGC 6 cut(s) 470, 542, 617, 706, 826, 869
Cfr13I GGNCC 2 cut(s) 674, 685
CviAII CATG 6 cut(s) 564, 650, 695, 797, 850, 890
DpnI GATC 3 cut(s) 397, 621, 629
DpnII GATC 3 cut(s) 395, 619, 627
EaeI YGGCCR 1 cut(s) 267
Eam1104I CTCTTC 2 cut(s) 44, 65
EarI CTCTTC 2 cut(s) 44, 65
Eco130I CCWWGG 1 cut(s) 460
Eco32I GATATC 2 cut(s) 232, 370
Eco47I GGWCC 1 cut(s) 685
Eco47III AGCGCT 1 cut(s) 616
Eco57I CTGAAG 1 cut(s) 644
Eco72I CACGTG 1 cut(s) 551
EcoO109I RGGNCCY 1 cut(s) 685
EcoRII CCWGG 3 cut(s) 676, 763, 856
EcoRV GATATC 2 cut(s) 232, 370
EcoT14I CCWWGG 1 cut(s) 460
ErhI CCWWGG 1 cut(s) 460
Esp3I CGTCTC 1 cut(s) 734
FaeI CATG 6 cut(s) 567, 653, 698, 800, 853, 893
FatI CATG 6 cut(s) 563, 649, 694, 796, 849, 889
Fnu4HI GCNGC 1 cut(s) 267
FokI GGATG 3 cut(s) 50, 167, 908
Fsp4HI GCNGC 1 cut(s) 267
FspBI CTAG 2 cut(s) 689, 815
FspI TGCGCA 1 cut(s) 825
GlaI GCGC 6 cut(s) 469, 541, 616, 705, 825, 868
GluI GCNGC 1 cut(s) 267
HaeII RGCGCY 1 cut(s) 618
HaeIII GGCC 4 cut(s) 269, 305, 478, 676
HapII CCGG 1 cut(s) 524
HhaI GCGC 6 cut(s) 470, 542, 617, 706, 826, 869
Hin1II CATG 6 cut(s) 567, 653, 698, 800, 853, 893
Hin6I GCGC 6 cut(s) 468, 540, 615, 704, 824, 867
HinP1I GCGC 6 cut(s) 468, 540, 615, 704, 824, 867
HincII GTYRAC 1 cut(s) 610
HindII GTYRAC 1 cut(s) 610
HindIII AAGCTT 1 cut(s) 915
HinfI GANTC 1 cut(s) 861
HpaII CCGG 1 cut(s) 524
HphI GGTGA 6 cut(s) 114, 176, 410, 485, 490, 656
Hpy166II GTNNAC 2 cut(s) 610, 647
Hpy188I TCNGA 2 cut(s) 100, 624
Hpy8I GTNNAC 2 cut(s) 610, 647
HpyAV CCTTC 1 cut(s) 833
HpyCH4III ACNGT 4 cut(s) 331, 439, 666, 787
HpyCH4IV ACGT 2 cut(s) 550, 743
HpyCH4V TGCA 4 cut(s) 89, 560, 796, 889
HpyF10VI GCNNNNNNNGC 4 cut(s) 449, 539, 566, 612
HpySE526I ACGT 2 cut(s) 550, 743
Hsp92II CATG 6 cut(s) 567, 653, 698, 800, 853, 893
HspAI GCGC 6 cut(s) 468, 540, 615, 704, 824, 867
Kzo9I GATC 3 cut(s) 395, 619, 627
LmnI GCTCC 1 cut(s) 842
LweI GCATC 5 cut(s) 20, 104, 189, 336, 721
MaeI CTAG 2 cut(s) 689, 815
MaeII ACGT 2 cut(s) 550, 743
MaeIII GTNAC 5 cut(s) 155, 164, 215, 544, 852
MalI GATC 3 cut(s) 397, 621, 629
MboI GATC 3 cut(s) 395, 619, 627
MboII GAAGA 5 cut(s) 31, 52, 448, 637, 803
MhlI GDGCHC 1 cut(s) 435
MluCI AATT 3 cut(s) 289, 801, 909
MmeI TCCRAC 1 cut(s) 240
MseI TTAA 1 cut(s) 912
MslI CAYNNNNRTG 6 cut(s) 162, 389, 654, 699, 729, 894
MspI CCGG 1 cut(s) 524
MspR9I CCNGG 4 cut(s) 524, 678, 765, 858
MvaI CCWGG 3 cut(s) 678, 765, 858
MvnI CGCG 1 cut(s) 867
MwoI GCNNNNNNNGC 4 cut(s) 449, 539, 566, 612
NciI CCSGG 1 cut(s) 524
NdeII GATC 3 cut(s) 395, 619, 627
NlaIII CATG 6 cut(s) 567, 653, 698, 800, 853, 893
NlaIV GGNNCC 4 cut(s) 382, 413, 520, 686
NmuCI GTSAC 2 cut(s) 155, 164
NsbI TGCGCA 1 cut(s) 825
NspI RCATGY 1 cut(s) 893
OliI CACNNNNGTG 1 cut(s) 162
PaeI GCATGC 1 cut(s) 893
PfeI GAWTC 1 cut(s) 861
PflMI CCANNNNNTGG 1 cut(s) 713
PkrI GCNGC 1 cut(s) 268
PmaCI CACGTG 1 cut(s) 551
PmlI CACGTG 1 cut(s) 551
Ppu21I YACGTR 1 cut(s) 551
PpuMI RGGWCCY 1 cut(s) 685
Psp5II RGGWCCY 1 cut(s) 685
Psp6I CCWGG 3 cut(s) 676, 763, 856
PspCI CACGTG 1 cut(s) 551
PspGI CCWGG 3 cut(s) 676, 763, 856
PspN4I GGNNCC 4 cut(s) 382, 413, 520, 686
PspPI GGNCC 2 cut(s) 674, 685
PspPPI RGGWCCY 1 cut(s) 685
PsrI GAACNNNNNNTAC 2 cut(s) 304, 336
RseI CAYNNNNRTG 6 cut(s) 162, 389, 654, 699, 729, 894
SaqAI TTAA 1 cut(s) 912
SatI GCNGC 1 cut(s) 267
Sau3AI GATC 3 cut(s) 395, 619, 627
Sau96I GGNCC 2 cut(s) 674, 685
ScrFI CCNGG 4 cut(s) 524, 678, 765, 858
SduI GDGCHC 1 cut(s) 435
SfaNI GCATC 5 cut(s) 20, 104, 189, 336, 721
SinI GGWCC 1 cut(s) 685
SmiMI CAYNNNNRTG 6 cut(s) 162, 389, 654, 699, 729, 894
SmlI CTYRAG 3 cut(s) 146, 299, 513
SmoI CTYRAG 3 cut(s) 146, 299, 513
SphI GCATGC 1 cut(s) 893
Sse9I AATT 3 cut(s) 289, 801, 909
SsiI CCGC 4 cut(s) 266, 387, 865, 893
SspMI CTAG 2 cut(s) 689, 815
StyD4I CCNGG 4 cut(s) 522, 676, 763, 856
StyI CCWWGG 1 cut(s) 460
TaaI ACNGT 4 cut(s) 331, 439, 666, 787
TaiI ACGT 2 cut(s) 553, 746
TaqI TCGA 3 cut(s) 204, 335, 352
TasI AATT 3 cut(s) 289, 801, 909
TauI GCSGC 1 cut(s) 269
TfiI GAWTC 1 cut(s) 861
Tru1I TTAA 1 cut(s) 912
Tru9I TTAA 1 cut(s) 912
TseFI GTSAC 2 cut(s) 155, 164
Tsp45I GTSAC 2 cut(s) 155, 164
TspDTI ATGAA 2 cut(s) 52, 813
Van91I CCANNNNNTGG 1 cut(s) 713
VpaK11BI GGWCC 1 cut(s) 685
XceI RCATGY 1 cut(s) 893
XcmI CCANNNNNNNNNTGG 1 cut(s) 313
XspI CTAG 2 cut(s) 689, 815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.