MD09G1040500.v1.1

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000309
Physical Location & Seq
Reverse (-)
2587282 .. 2587791
510 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1040500.v1.1.491

Sequence Viewer

Length: 510 bp
ATGTTCGCCTCACACAGTTGCTCATTATTTCTTTTTACCGGAAACCCAAAATGGCCTTCTTCTAAGTACCATCATCTCACCTATGCTTTTGATCAAAGCACCCCAACTGAGGCCCGGGACGCAGTTGCGCGCGCTTTCGCAACATGGCAGGACACACACTTCTCGTTCAGTCAATCCCAAAACTATAAGAACGCGGATCTGACAATAAGTTTTGGTAAGGGTGATCATGGAGATGGGAACCCATTTGACGGCCGAGGTGGGATCCTTGCTCACGCATTTCCACCGACCAATGAGAGATTCCACTACGATGCCGATGAGGCGTGGGCTGTGGGAGCTGTGGCGGACGCTTATGACTTGGAGACTGTGGCCTTGCATGAAATCGGACATCTGTTGGGGCTACAACATAGCTCTGTTGAAGGAGCCGTCATGTCCCCTGGCGTTCGTAGTGGATTTACCCAAAGTTTGCATGCAGATGATATTCAAGGAATTAAAGCTTTATATAACACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

170

Amino Acids

18.46

Weight (kDa)

5.53

Isoelectric Point (pI)

38.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M10 PF00413 18 - 167 1.8e-45 Matrixin
Reprolysin_3 PF13582 30 - 136 8.2e-06 Metallo-peptidase family M12B Reprolysin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 130, 132, 194
AciI CCGC 2 cut(s) 194, 341
AclWI GGATC 3 cut(s) 204, 256, 269
AcoI YGGCCR 1 cut(s) 250
AfaI GTAC 1 cut(s) 68
AfiI CCNNNNNNNGG 2 cut(s) 109, 248
AgsI TTSAA 2 cut(s) 416, 482
AjnI CCWGG 1 cut(s) 433
AjuI GAANNNNNNNTTGG 4 cut(s) 40, 72, 281, 313
AluBI AGCT 3 cut(s) 335, 408, 494
AluI AGCT 3 cut(s) 335, 408, 494
Alw26I GTCTC 1 cut(s) 353
AlwI GGATC 3 cut(s) 204, 256, 269
Ama87I CYCGRG 1 cut(s) 114
AoxI GGCC 4 cut(s) 53, 111, 250, 366
AspLEI GCGC 3 cut(s) 130, 132, 134
AspS9I GGNCC 1 cut(s) 112
AsuC2I CCSGG 2 cut(s) 115, 116
AsuHPI GGTGA 2 cut(s) 70, 233
AvaI CYCGRG 1 cut(s) 114
BamHI GGATCC 1 cut(s) 261
BccI CCATC 2 cut(s) 78, 227
BceAI ACGGC 2 cut(s) 265, 407
BciT130I CCWGG 1 cut(s) 435
BclI TGATCA 2 cut(s) 91, 223
BcnI CCSGG 2 cut(s) 115, 116
BcoDI GTCTC 1 cut(s) 353
BglI GCCNNNNNGGC 1 cut(s) 317
Bme1390I CCNGG 3 cut(s) 115, 116, 435
BmeT110I CYCGRG 1 cut(s) 114
BmgT120I GGNCC 1 cut(s) 112
BmiI GGNNCC 3 cut(s) 239, 263, 421
BmrFI CCNGG 3 cut(s) 115, 116, 435
BmsI GCATC 1 cut(s) 298
BpuMI CCSGG 2 cut(s) 115, 116
BsaJI CCNNGG 3 cut(s) 114, 253, 433
BsaWI WCCGGW 1 cut(s) 38
Bsc4I CCNNNNNNNGG 2 cut(s) 109, 248
BseBI CCWGG 1 cut(s) 435
BseDI CCNNGG 3 cut(s) 114, 253, 433
BseLI CCNNNNNNNGG 2 cut(s) 109, 248
BseMII CTCAG 1 cut(s) 99
BsePI GCGCGC 2 cut(s) 128, 130
BseX3I CGGCCG 1 cut(s) 250
Bsh1236I CGCG 3 cut(s) 130, 132, 194
Bsh1285I CGRYCG 1 cut(s) 253
BshFI GGCC 4 cut(s) 55, 113, 252, 368
BsiEI CGRYCG 1 cut(s) 253
BsiHKCI CYCGRG 1 cut(s) 114
BsiSI CCGG 2 cut(s) 39, 115
BslFI GGGAC 2 cut(s) 131, 415
BslI CCNNNNNNNGG 2 cut(s) 109, 248
BsmAI GTCTC 1 cut(s) 353
BsmFI GGGAC 2 cut(s) 131, 415
BsnI GGCC 4 cut(s) 55, 113, 252, 368
BsoBI CYCGRG 1 cut(s) 114
Bsp143I GATC 4 cut(s) 91, 196, 223, 261
BspACI CCGC 2 cut(s) 194, 341
BspANI GGCC 4 cut(s) 55, 113, 252, 368
BspCNI CTCAG 1 cut(s) 100
BspFNI CGCG 3 cut(s) 130, 132, 194
BspLI GGNNCC 3 cut(s) 239, 263, 421
BspPI GGATC 3 cut(s) 204, 256, 269
BssECI CCNNGG 3 cut(s) 114, 253, 433
BssHII GCGCGC 2 cut(s) 128, 130
BssMI GATC 4 cut(s) 91, 196, 223, 261
Bst2UI CCWGG 1 cut(s) 435
Bst4CI ACNGT 2 cut(s) 17, 364
BstC8I GCNNGC 3 cut(s) 130, 132, 468
BstDEI CTNAG 2 cut(s) 63, 108
BstFNI CGCG 3 cut(s) 130, 132, 194
BstHHI GCGC 3 cut(s) 130, 132, 134
BstKTI GATC 4 cut(s) 94, 199, 226, 264
BstMAI GTCTC 1 cut(s) 353
BstMBI GATC 4 cut(s) 91, 196, 223, 261
BstMCI CGRYCG 1 cut(s) 253
BstMWI GCNNNNNNNGC 3 cut(s) 119, 317, 332
BstNI CCWGG 1 cut(s) 435
BstNSI RCATGY 1 cut(s) 470
BstSCI CCNGG 3 cut(s) 113, 114, 433
BstUI CGCG 3 cut(s) 130, 132, 194
BstX2I RGATCY 2 cut(s) 196, 261
BstYI RGATCY 2 cut(s) 196, 261
BstZI CGGCCG 1 cut(s) 250
BsuRI GGCC 4 cut(s) 55, 113, 252, 368
Cac8I GCNNGC 3 cut(s) 130, 132, 468
CfoI GCGC 3 cut(s) 130, 132, 134
Cfr13I GGNCC 1 cut(s) 112
Cfr9I CCCGGG 1 cut(s) 114
CseI GACGC 2 cut(s) 128, 353
Csp6I GTAC 1 cut(s) 67
CviAII CATG 5 cut(s) 144, 227, 374, 427, 467
CviQI GTAC 1 cut(s) 67
DdeI CTNAG 2 cut(s) 63, 108
DpnI GATC 4 cut(s) 93, 198, 225, 263
DpnII GATC 4 cut(s) 91, 196, 223, 261
EaeI YGGCCR 1 cut(s) 250
EagI CGGCCG 1 cut(s) 250
EciI GGCGGA 1 cut(s) 356
EclXI CGGCCG 1 cut(s) 250
Eco52I CGGCCG 1 cut(s) 250
Eco88I CYCGRG 1 cut(s) 114
EcoRII CCWGG 1 cut(s) 433
FaeI CATG 5 cut(s) 147, 230, 377, 430, 470
FaqI GGGAC 2 cut(s) 131, 415
FatI CATG 5 cut(s) 143, 226, 373, 426, 466
FbaI TGATCA 2 cut(s) 91, 223
GlaI GCGC 3 cut(s) 129, 131, 133
HaeIII GGCC 4 cut(s) 55, 113, 252, 368
HapII CCGG 2 cut(s) 39, 115
HgaI GACGC 2 cut(s) 128, 353
HhaI GCGC 3 cut(s) 130, 132, 134
Hin1II CATG 5 cut(s) 147, 230, 377, 430, 470
Hin6I GCGC 3 cut(s) 128, 130, 132
HinP1I GCGC 3 cut(s) 128, 130, 132
HindIII AAGCTT 1 cut(s) 492
HinfI GANTC 1 cut(s) 297
HpaII CCGG 2 cut(s) 39, 115
HphI GGTGA 2 cut(s) 70, 233
Hpy188I TCNGA 2 cut(s) 201, 383
HpyAV CCTTC 2 cut(s) 66, 410
HpyCH4III ACNGT 2 cut(s) 17, 364
HpyCH4V TGCA 3 cut(s) 373, 466, 470
HpyF10VI GCNNNNNNNGC 3 cut(s) 119, 317, 332
HpyF3I CTNAG 2 cut(s) 63, 108
Hsp92II CATG 5 cut(s) 147, 230, 377, 430, 470
HspAI GCGC 3 cut(s) 128, 130, 132
Ksp22I TGATCA 2 cut(s) 91, 223
Kzo9I GATC 4 cut(s) 91, 196, 223, 261
LmnI GCTCC 2 cut(s) 332, 419
LpnPI CCDG 5 cut(s) 52, 128, 134, 420, 447
LweI GCATC 1 cut(s) 298
MalI GATC 4 cut(s) 93, 198, 225, 263
MboI GATC 4 cut(s) 91, 196, 223, 261
MboII GAAGA 1 cut(s) 51
MflI RGATCY 2 cut(s) 196, 261
MluCI AATT 1 cut(s) 486
MnlI CCTC 4 cut(s) 19, 103, 248, 310
MseI TTAA 1 cut(s) 489
MslI CAYNNNNRTG 3 cut(s) 231, 306, 471
MspI CCGG 2 cut(s) 39, 115
MspR9I CCNGG 3 cut(s) 115, 116, 435
MvaI CCWGG 1 cut(s) 435
MvnI CGCG 3 cut(s) 130, 132, 194
MwoI GCNNNNNNNGC 3 cut(s) 119, 317, 332
NciI CCSGG 2 cut(s) 115, 116
NdeII GATC 4 cut(s) 91, 196, 223, 261
NlaIII CATG 5 cut(s) 147, 230, 377, 430, 470
NlaIV GGNNCC 3 cut(s) 239, 263, 421
NmeAIII GCCGAG 1 cut(s) 278
NspI RCATGY 1 cut(s) 470
PaeI GCATGC 1 cut(s) 470
PauI GCGCGC 2 cut(s) 128, 130
PfeI GAWTC 1 cut(s) 297
Psp6I CCWGG 1 cut(s) 433
PspGI CCWGG 1 cut(s) 433
PspN4I GGNNCC 3 cut(s) 239, 263, 421
PspPI GGNCC 1 cut(s) 112
PsuI RGATCY 2 cut(s) 196, 261
PteI GCGCGC 2 cut(s) 128, 130
RsaI GTAC 1 cut(s) 68
RsaNI GTAC 1 cut(s) 67
RseI CAYNNNNRTG 3 cut(s) 231, 306, 471
SaqAI TTAA 1 cut(s) 489
Sau3AI GATC 4 cut(s) 91, 196, 223, 261
Sau96I GGNCC 1 cut(s) 112
ScrFI CCNGG 3 cut(s) 115, 116, 435
SetI ASST 5 cut(s) 83, 259, 337, 410, 496
SfaNI GCATC 1 cut(s) 298
SmaI CCCGGG 1 cut(s) 116
SmiMI CAYNNNNRTG 3 cut(s) 231, 306, 471
SphI GCATGC 1 cut(s) 470
Sse9I AATT 1 cut(s) 486
SsiI CCGC 2 cut(s) 194, 341
StyD4I CCNGG 3 cut(s) 113, 114, 433
TaaI ACNGT 2 cut(s) 17, 364
TasI AATT 1 cut(s) 486
TfiI GAWTC 1 cut(s) 297
Tru1I TTAA 1 cut(s) 489
Tru9I TTAA 1 cut(s) 489
TspDTI ATGAA 1 cut(s) 390
TspMI CCCGGG 1 cut(s) 114
XceI RCATGY 1 cut(s) 470
XmaI CCCGGG 1 cut(s) 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.