Rw7G030120

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000309
Physical Location & Seq
Forward (+)
42522018 .. 42523203
1186 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G030120.1

Sequence Viewer

Length: 885 bp
ATGGCATCCAAATCCAATCTTTCTCTCTTCACCATCACTCTCCTCCTTGCCCTTCTTACTCTCCTCTCTTATGCAACTGCTTCTCATCCCCACAACAAGAGCTCTTCCCCATTTGAATTCCTTGAGCATCTCAAGGGTTGCCACAAAGGTGATAAGGTCAAGGGCATCAATGACCTCAAGAAATACCTTGAAAAGTACGGTTACTTGAAATACAAGAATCACACCATCAAGACTTACCAGATCAATTACCACCTCAAGTCCACCGGAACATTGGATGGCAAAACAGTGTCGAAGATGATGACGCCTTGTTGTGGCGTGCCGGATATCATCAATGGCACCTCGTCCATGCGATCAGCCAAGAAAAGGCACCACCATGGTTCAATTCATACCACTGCTCATTACTCATTTTTTGAGGGAAATCTAAAATGGCCTTCCTCTAAATACCATCTCACTTACGGTTTCCTCCCCAACACCCCATCCGAAGCCACGAGCGCTGTCGCACGGGCTTTCGCAACATGGGCTGGGAACACTCACTTCACGTTTAGCCAGTCACAAAGTTACGAGAGTTCGGATTTGAAGATTAGTTTTCATAAGAGTGATCATGGAGACGGGAATCCATTTTATGGCCCCGGCGGGATCCTAGCCCATGCTTATAGCCCGTCGGATGGAAGATTCCACTACGACGCAGATGAGACTTGGTCTGTGGGTGCTACACCAGGCGCTTATGACTTGGAGACGGTGGCTTTGCATGAAATAGGGCACCTTCTGGGACTTGGACATAGCTCAGTGGAGGGAGCTATAATGGCCCCAGGTATTCCTCCGGGAGTGACTCGAAGTTTGCATGGGGACGATGTTCAAGGAATCAAGGCTTTATACAACGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

294

Amino Acids

32.15

Weight (kDa)

8.74

Isoelectric Point (pI)

32.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M10 PF00413 142 - 292 3.3e-44 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 335, 366, 759
AccB7I CCANNNNNTGG 1 cut(s) 623
AciI CCGC 1 cut(s) 633
AclWI GGATC 2 cut(s) 631, 644
AcsI RAATTY 1 cut(s) 116
AcyI GRCGYC 1 cut(s) 302
AfaI GTAC 1 cut(s) 197
AfeI AGCGCT 1 cut(s) 493
AfiI CCNNNNNNNGG 4 cut(s) 311, 363, 623, 665
AgsI TTSAA 6 cut(s) 116, 191, 208, 381, 577, 857
AjnI CCWGG 2 cut(s) 715, 808
AleI CACNNNNGTG 1 cut(s) 147
AluBI AGCT 3 cut(s) 102, 783, 797
AluI AGCT 3 cut(s) 102, 783, 797
Alw21I GWGCWC 1 cut(s) 104
Alw26I GTCTC 3 cut(s) 600, 686, 728
AlwI GGATC 2 cut(s) 631, 644
Aor51HI AGCGCT 1 cut(s) 493
AoxI GGCC 3 cut(s) 428, 625, 804
ApoI RAATTY 1 cut(s) 116
ArsI GACNNNNNNTTYG 4 cut(s) 727, 759, 820, 852
AspLEI GCGC 2 cut(s) 494, 722
AspS9I GGNCC 2 cut(s) 626, 805
AsuC2I CCSGG 2 cut(s) 630, 822
AsuHPI GGTGA 2 cut(s) 22, 161
BaeGI GKGCMC 1 cut(s) 762
BamHI GGATCC 1 cut(s) 636
BanI GGYRCC 3 cut(s) 335, 366, 759
BanII GRGCYC 1 cut(s) 104
BauI CACGAG 1 cut(s) 487
Bbv12I GWGCWC 1 cut(s) 104
BccI CCATC 6 cut(s) 41, 233, 269, 453, 484, 659
BciT130I CCWGG 2 cut(s) 717, 810
BclI TGATCA 1 cut(s) 598
BcnI CCSGG 2 cut(s) 630, 822
BcoDI GTCTC 3 cut(s) 600, 686, 728
BfaI CTAG 1 cut(s) 641
BfoI RGCGCY 2 cut(s) 495, 723
Bme1390I CCNGG 4 cut(s) 630, 717, 810, 822
BmgT120I GGNCC 2 cut(s) 626, 805
BmiI GGNNCC 6 cut(s) 337, 368, 628, 638, 761, 807
BmrFI CCNGG 4 cut(s) 630, 717, 810, 822
BmsI GCATC 3 cut(s) 14, 136, 174
BpuEI CTTGAG 4 cut(s) 116, 143, 161, 239
BpuMI CCSGG 2 cut(s) 630, 822
BsaHI GRCGYC 1 cut(s) 302
BsaJI CCNNGG 3 cut(s) 373, 628, 808
BsaWI WCCGGW 1 cut(s) 263
Bsc4I CCNNNNNNNGG 4 cut(s) 311, 363, 623, 665
Bse1I ACTGG 1 cut(s) 547
BseBI CCWGG 2 cut(s) 717, 810
BseDI CCNNGG 3 cut(s) 373, 628, 808
BseGI GGATG 5 cut(s) 5, 85, 280, 476, 670
BseLI CCNNNNNNNGG 4 cut(s) 311, 363, 623, 665
BseMII CTCAG 1 cut(s) 798
BseNI ACTGG 1 cut(s) 547
BseRI GAGGAG 2 cut(s) 32, 53
BseSI GKGCMC 1 cut(s) 762
BseYI CCCAGC 1 cut(s) 521
BshFI GGCC 3 cut(s) 430, 627, 806
BshNI GGYRCC 3 cut(s) 335, 366, 759
BsiHKAI GWGCWC 1 cut(s) 104
BsiSI CCGG 4 cut(s) 264, 320, 630, 821
BslFI GGGAC 2 cut(s) 783, 860
BslI CCNNNNNNNGG 4 cut(s) 311, 363, 623, 665
BsmAI GTCTC 3 cut(s) 600, 686, 728
BsmBI CGTCTC 2 cut(s) 600, 728
BsmFI GGGAC 2 cut(s) 783, 860
BsnI GGCC 3 cut(s) 430, 627, 806
Bsp1286I GDGCHC 2 cut(s) 104, 762
Bsp143I GATC 4 cut(s) 240, 350, 598, 636
Bsp19I CCATGG 1 cut(s) 373
BspACI CCGC 1 cut(s) 633
BspANI GGCC 3 cut(s) 430, 627, 806
BspCNI CTCAG 1 cut(s) 797
BspLI GGNNCC 6 cut(s) 337, 368, 628, 638, 761, 807
BspPI GGATC 2 cut(s) 631, 644
BspQI GCTCTTC 1 cut(s) 109
BspT107I GGYRCC 3 cut(s) 335, 366, 759
BsrI ACTGG 1 cut(s) 547
BssECI CCNNGG 3 cut(s) 373, 628, 808
BssMI GATC 4 cut(s) 240, 350, 598, 636
BssNI GRCGYC 1 cut(s) 302
BssSI CACGAG 1 cut(s) 487
BssT1I CCWWGG 1 cut(s) 373
Bst2BI CACGAG 1 cut(s) 487
Bst2UI CCWGG 2 cut(s) 717, 810
Bst4CI ACNGT 4 cut(s) 200, 286, 458, 739
Bst6I CTCTTC 2 cut(s) 32, 109
BstACI GRCGYC 1 cut(s) 302
BstC8I GCNNGC 1 cut(s) 317
BstDEI CTNAG 1 cut(s) 784
BstDSI CCRYGG 1 cut(s) 373
BstF5I GGATG 5 cut(s) 5, 85, 280, 476, 670
BstH2I RGCGCY 2 cut(s) 495, 723
BstHHI GCGC 2 cut(s) 494, 722
BstKTI GATC 4 cut(s) 243, 353, 601, 639
BstMAI GTCTC 3 cut(s) 600, 686, 728
BstMBI GATC 4 cut(s) 240, 350, 598, 636
BstMWI GCNNNNNNNGC 3 cut(s) 491, 518, 803
BstNI CCWGG 2 cut(s) 717, 810
BstSCI CCNGG 4 cut(s) 628, 715, 808, 820
BstSLI GKGCMC 1 cut(s) 762
BstX2I RGATCY 1 cut(s) 636
BstYI RGATCY 1 cut(s) 636
BsuRI GGCC 3 cut(s) 430, 627, 806
BtgI CCRYGG 1 cut(s) 373
BtsCI GGATG 5 cut(s) 5, 85, 280, 476, 670
BtsI GCAGTG 1 cut(s) 390
BtsIMutI CAGTG 3 cut(s) 291, 390, 792
Cac8I GCNNGC 1 cut(s) 317
CfoI GCGC 2 cut(s) 494, 722
Cfr13I GGNCC 2 cut(s) 626, 805
CseI GACGC 2 cut(s) 310, 692
Csp6I GTAC 1 cut(s) 196
CviAII CATG 7 cut(s) 346, 374, 516, 602, 647, 749, 842
CviQI GTAC 1 cut(s) 196
DdeI CTNAG 1 cut(s) 784
DpnI GATC 4 cut(s) 242, 352, 600, 638
DpnII GATC 4 cut(s) 240, 350, 598, 636
Eam1104I CTCTTC 2 cut(s) 32, 109
EarI CTCTTC 2 cut(s) 32, 109
Ecl136II GAGCTC 1 cut(s) 102
Eco130I CCWWGG 1 cut(s) 373
Eco24I GRGCYC 1 cut(s) 104
Eco32I GATATC 1 cut(s) 325
Eco47III AGCGCT 1 cut(s) 493
Eco53kI GAGCTC 1 cut(s) 102
EcoICRI GAGCTC 1 cut(s) 102
EcoRI GAATTC 1 cut(s) 116
EcoRII CCWGG 2 cut(s) 715, 808
EcoRV GATATC 1 cut(s) 325
EcoT14I CCWWGG 1 cut(s) 373
EcoT38I GRGCYC 1 cut(s) 104
ErhI CCWWGG 1 cut(s) 373
Esp3I CGTCTC 2 cut(s) 600, 728
FaeI CATG 7 cut(s) 349, 377, 519, 605, 650, 752, 845
FaqI GGGAC 2 cut(s) 783, 860
FatI CATG 7 cut(s) 345, 373, 515, 601, 646, 748, 841
FauI CCCGC 1 cut(s) 626
FbaI TGATCA 1 cut(s) 598
FokI GGATG 4 cut(s) 72, 287, 463, 677
FriOI GRGCYC 1 cut(s) 104
FspBI CTAG 1 cut(s) 641
GlaI GCGC 2 cut(s) 493, 721
GsaI CCCAGC 1 cut(s) 525
HaeII RGCGCY 2 cut(s) 495, 723
HaeIII GGCC 3 cut(s) 430, 627, 806
HapII CCGG 4 cut(s) 264, 320, 630, 821
HgaI GACGC 2 cut(s) 310, 692
HhaI GCGC 2 cut(s) 494, 722
Hin1I GRCGYC 1 cut(s) 302
Hin1II CATG 7 cut(s) 349, 377, 519, 605, 650, 752, 845
Hin6I GCGC 2 cut(s) 492, 720
HinP1I GCGC 2 cut(s) 492, 720
HinfI GANTC 5 cut(s) 217, 613, 672, 829, 861
HpaII CCGG 4 cut(s) 264, 320, 630, 821
HphI GGTGA 2 cut(s) 22, 161
Hpy166II GTNNAC 1 cut(s) 261
Hpy188I TCNGA 4 cut(s) 481, 571, 664, 884
Hpy188III TCNNGA 2 cut(s) 178, 229
Hpy8I GTNNAC 1 cut(s) 261
Hpy99I CGWCG 2 cut(s) 664, 686
HpyAV CCTTC 3 cut(s) 62, 441, 773
HpyCH4III ACNGT 4 cut(s) 200, 286, 458, 739
HpyCH4IV ACGT 2 cut(s) 539, 879
HpyCH4V TGCA 3 cut(s) 74, 748, 841
HpyF10VI GCNNNNNNNGC 3 cut(s) 491, 518, 803
HpyF3I CTNAG 1 cut(s) 784
HpySE526I ACGT 2 cut(s) 539, 879
Hsp92I GRCGYC 1 cut(s) 302
Hsp92II CATG 7 cut(s) 349, 377, 519, 605, 650, 752, 845
HspAI GCGC 2 cut(s) 492, 720
Ksp22I TGATCA 1 cut(s) 598
Kzo9I GATC 4 cut(s) 240, 350, 598, 636
LguI GCTCTTC 1 cut(s) 109
LmnI GCTCC 1 cut(s) 794
LweI GCATC 3 cut(s) 14, 136, 174
MaeI CTAG 1 cut(s) 641
MaeII ACGT 2 cut(s) 539, 879
MaeIII GTNAC 4 cut(s) 200, 549, 557, 826
MalI GATC 4 cut(s) 242, 352, 600, 638
MboI GATC 4 cut(s) 240, 350, 598, 636
MboII GAAGA 5 cut(s) 19, 96, 304, 589, 681
MflI RGATCY 1 cut(s) 636
MhlI GDGCHC 2 cut(s) 104, 762
MluCI AATT 3 cut(s) 116, 244, 381
MlyI GAGTC 1 cut(s) 823
MmeI TCCRAC 1 cut(s) 642
MslI CAYNNNNRTG 3 cut(s) 147, 372, 594
MspI CCGG 4 cut(s) 264, 320, 630, 821
MspR9I CCNGG 4 cut(s) 630, 717, 810, 822
MvaI CCWGG 2 cut(s) 717, 810
MwoI GCNNNNNNNGC 3 cut(s) 491, 518, 803
NciI CCSGG 2 cut(s) 630, 822
NcoI CCATGG 1 cut(s) 373
NdeII GATC 4 cut(s) 240, 350, 598, 636
NlaIII CATG 7 cut(s) 349, 377, 519, 605, 650, 752, 845
NlaIV GGNNCC 6 cut(s) 337, 368, 628, 638, 761, 807
NmuCI GTSAC 2 cut(s) 549, 826
OliI CACNNNNGTG 1 cut(s) 147
PciSI GCTCTTC 1 cut(s) 109
PfeI GAWTC 4 cut(s) 217, 613, 672, 861
PflFI GACNNNGTC 1 cut(s) 697
PflMI CCANNNNNTGG 1 cut(s) 623
PfoI TCCNGGA 1 cut(s) 820
PleI GAGTC 1 cut(s) 823
PpsI GAGTC 1 cut(s) 823
Psp124BI GAGCTC 1 cut(s) 104
Psp6I CCWGG 2 cut(s) 715, 808
PspFI CCCAGC 1 cut(s) 521
PspGI CCWGG 2 cut(s) 715, 808
PspN4I GGNNCC 6 cut(s) 337, 368, 628, 638, 761, 807
PspPI GGNCC 2 cut(s) 626, 805
PsuI RGATCY 1 cut(s) 636
PsyI GACNNNGTC 1 cut(s) 697
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
RseI CAYNNNNRTG 3 cut(s) 147, 372, 594
SacI GAGCTC 1 cut(s) 104
SapI GCTCTTC 1 cut(s) 109
Sau3AI GATC 4 cut(s) 240, 350, 598, 636
Sau96I GGNCC 2 cut(s) 626, 805
SchI GAGTC 1 cut(s) 823
ScrFI CCNGG 4 cut(s) 630, 717, 810, 822
SduI GDGCHC 2 cut(s) 104, 762
SfaNI GCATC 3 cut(s) 14, 136, 174
SmiMI CAYNNNNRTG 3 cut(s) 147, 372, 594
SmlI CTYRAG 4 cut(s) 122, 131, 176, 254
SmoI CTYRAG 4 cut(s) 122, 131, 176, 254
Sse9I AATT 3 cut(s) 116, 244, 381
SsiI CCGC 1 cut(s) 633
SspMI CTAG 1 cut(s) 641
SstI GAGCTC 1 cut(s) 104
StyD4I CCNGG 4 cut(s) 628, 715, 808, 820
StyI CCWWGG 1 cut(s) 373
TaaI ACNGT 4 cut(s) 200, 286, 458, 739
TaiI ACGT 2 cut(s) 542, 882
TaqI TCGA 2 cut(s) 290, 832
TasI AATT 3 cut(s) 116, 244, 381
TfiI GAWTC 4 cut(s) 217, 613, 672, 861
TscAI CASTG 3 cut(s) 291, 397, 792
TseFI GTSAC 2 cut(s) 549, 826
Tsp45I GTSAC 2 cut(s) 549, 826
TspDTI ATGAA 3 cut(s) 374, 578, 765
TspRI CASTG 3 cut(s) 291, 397, 792
Tth111I GACNNNGTC 1 cut(s) 697
Van91I CCANNNNNTGG 1 cut(s) 623
XapI RAATTY 1 cut(s) 116
XcmI CCANNNNNNNNNTGG 1 cut(s) 268
XspI CTAG 1 cut(s) 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.