MD05G1034100.v1.1

Dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000167
Physical Location & Seq
Forward (+)
5462893 .. 5466016
3124 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1034100.v1.1.491

Sequence Viewer

Length: 1080 bp
ATGGTGGAATCTCCAGAACAACAACACCCCAAGAAAGCCTTTGGATGGGCTGCCAGAGACTCATCTGGTGTTCTCTCTCCCTTCAAATTCTCCAGAAGGGAACCAGGAAAGAACGACGTCACGTTCAAAGTATTATACTGTGGAATATGCCATACAGATCTTGACATGATTAAGAATGAATGGGGAAATTCTACCTATCCTCTACTTCCGGGGCACGAGATTGTTGGTGTAGTAACTGAGGTAGGCATCAAAGTAGAGAAATTCAAAGTTGGAGACCATGTCGGTGTTGGATATATGGCAGGATCTTGCCAATCTTGTGATAGTTGTTCCACCAATGCTGAGAATTACTGCCCCAAAATGATAGCGACTTTTGGTGACATATACCATGACGGAACCCCAACATATGGAGGTTACTCTGACATCATGGTCGCCAATGAGCAATTTGTAGTCCGTATTCCAGGCAGTCTACCTCTCGATGGCGCTGCTCCTCTCCTATGTGCTGGGGTTACAACTTACAGCCCCTTGAGGTATTTCGGACTTGATAAACCCGGTATGCATGTGGGTGTGGTTGGCGTCGGTGGCCTAGGTCACGTTGCCCTGAAGTTTGCAAAGGCTATGGGGCTCAAGGTCACAATGATCAGTACCTCCCCGAAGAAGAAAGAGGAAGCTATTGAAAACCTTGGTGCCGATTCCTTTTTGATCAGCCACGACGATGGCCAAATGCAGGGTGCCGTGGGCACAATGGATGGGATCATTGACACTGTTTCTGCAGTACACCCTCTCTTGCCTTTGGTTGGACTATTGAAGTCTAATGGAAAGCTTGTTCTGCTTGGAGTACCAGATAAGCCTCTTCAGCTACCAGTTTTTCCTTTGCTCATGGGGAGAAAGATAATAGCTGGTAGTTCTATTGGAGGAATGAAGGAGACCCAAGAGATGTTTGATTTCGCAGCTAAGCACAACATAACAGCGAACGTTGAAGTTATATCAGTTGATTATGCAAATACAGCTATGGAACGTCTTCTCAAAGGAGACATTAGATACCGTTTCGTCATTGACATTGGGAACACATTGCACGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

360

Amino Acids

38.58

Weight (kDa)

6.26

Isoelectric Point (pI)

30.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 36 - 153 2.7e-25 Alcohol dehydrogenase GroES-like domain
ADH_zinc_N PF00107 194 - 317 3.6e-19 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000167)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G37980 AT4G37980 AT4G37990
fragaria_vesca FvH4_1g19210 FvH4_1g19220 FvH4_1g19240 FvH4_1g19240 FvH4_1g19261 FvH4_1g19262 FvH4_1g19263 FvH4_1g19263 FvH4_1g19265 FvH4_1g19281 FvH4_1g19281 FvH4_7g07240 FvH4_7g07240 FvH4_7g07240
malus_domestica MD01G1042500.v1.1 MD01G1042600.v1.1 MD01G1042800.v1.1 MD01G1042900.v1.1 MD05G1034100.v1.1 MD10G1035900.v1.1 MD10G1036000.v1.1 MD10G1155000.v1.1 MD10G1155100.v1.1 MD10G1155200.v1.1 MD10G1155400.v1.1 MD10G1155700.v1.1 MD10G1155800.v1.1 MD15G1308800.v1.1 MD15G1308900.v1.1
prunus_persica Prupe.6G205600_v2.0.a1 Prupe.6G205700_v2.0.a1 Prupe.6G205800_v2.0.a1 Prupe.6G205900_v2.0.a1 Prupe.6G206300_v2.0.a1 Prupe.6G206800_v2.0.a1 Prupe.6G207100_v2.0.a1 Prupe.6G207200_v2.0.a1 Prupe.6G207300_v2.0.a1 Prupe.6G207400_v2.0.a1 Prupe.6G207500_v2.0.a1 Prupe.6G207600_v2.0.a1 Prupe.6G207700_v2.0.a1 Prupe.6G207900_v2.0.a1
pyrus_communis pycom01g06970 pycom01g06980 pycom01g06990 pycom05g02460 pycom08g00750 pycom10g13410 pycom15g27310 pycom15g27320
rosa_chinensis RchiOBHm_Chr1g0314861 RchiOBHm_Chr1g0345441 RchiOBHm_Chr1g0345451 RchiOBHm_Chr1g0345621 RchiOBHm_Chr1g0345751 RchiOBHm_Chr1g0345861 RchiOBHm_Chr1g0345871 RchiOBHm_Chr1g0345891 RchiOBHm_Chr2g0094851 RchiOBHm_Chr2g0110471 RchiOBHm_Chr2g0110511 RchiOBHm_Chr2g0110531 RchiOBHm_Chr2g0110541 RchiOBHm_Chr2g0110571 RchiOBHm_Chr5g0039381 RchiOBHm_Chr5g0039391 RchiOBHm_Chr6g0270311 RchiOBHm_Chr6g0270331 RchiOBHm_Chr6g0270411 RchiOBHm_Chr6g0270431
rosa_laevigata RLG00000000515 RLG00000013793 RLG00000013797 RLG00000013799 RLG00000016525 RLG00000017808 RLG00000017811 RLG00000017813 RLG00000017816 RLG00000017819 RLG00000017822 RLG00000017824 RLG00000017825 RLG00000028835
rosa_multiflora Rmu_co8225458.1_g000001 Rmu_sc0000389.1_g000006 Rmu_sc0000389.1_g000014 Rmu_sc0000389.1_g000023 Rmu_sc0001292.1_g000012 Rmu_sc0001292.1_g000026 Rmu_sc0002145.1_g000003 Rmu_sc0002329.1_g000033 Rmu_sc0002833.1_g000044 Rmu_sc0004316.1_g000012 Rmu_sc0004439.1_g000003 Rmu_sc0006422.1_g000028 Rmu_sc0009479.1_g000012 Rmu_sc0010489.1_g000009 Rmu_sc0010489.1_g000010 Rmu_sc0017837.1_g000003 Rmu_sc0032320.1_g000001
rosa_roxburghii Rroxscaffold_1G00050770 Rroxscaffold_2G00133110 Rroxscaffold_2G00133140 Rroxscaffold_2G00133200 Rroxscaffold_2G00133270 Rroxscaffold_2G00133290 Rroxscaffold_2G00133300 Rroxscaffold_2G00133350 Rroxscaffold_4G00292490 Rroxscaffold_4G00309020 Rroxscaffold_4G00331740 Rroxscaffold_4G00331800 Rroxscaffold_6G00404470 Rroxscaffold_7G00197950 Rroxscaffold_7G00197990 Rroxscaffold_7G00198020
rosa_rugosa Rorug01G0002500 Rorug02G0047200 Rorug02G0047300 Rorug02G0047300 Rorug02G0047400 Rorug02G0168000 Rorug02G0168000 Rorug02G0168100 Rorug02G0168100 Rorug02G0168400.1 Rorug03G0157600 Rorug03G0157700 Rorug05G0112600 Rorug06G0054600 Rorug06G0054700
rosa_samantha Rh1AG013300 Rh1AG195600 Rh1AG195700 Rh1AG196000 Rh1BG004900 Rh1BG114300 Rh2AG132300 Rh2BG095500 Rh2BG229700 Rh2BG229800 Rh2CG097400 Rh2CG221500 Rh2CG221800 Rh2CG222100 Rh2CG222200 Rh2CG222500 Rh2CG222600 Rh2CG345000 Rh5BG269700 Rh6AG172900 Rh6AG173100 Rh6CG080500 Rh6DG075900 Rh6DG164700 Rh6DG164900 Rh6DG165200 Rh6DG165300
rosa_wichuraiana Rw0G015390 Rw1G000600 Rw1G016130 Rw1G016190 Rw2G007140 Rw2G016970 Rw2G016990 Rw2G017000 Rw3G018910 Rw3G018920 Rw3G018930 Rw5G024830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 425
AatII GACGTC 1 cut(s) 120
AccB1I GGYRCC 2 cut(s) 683, 728
AccB7I CCANNNNNTGG 1 cut(s) 404
AccI GTMKAC 1 cut(s) 466
AclI AACGTT 1 cut(s) 972
AclWI GGATC 2 cut(s) 310, 758
AcoI YGGCCR 1 cut(s) 715
AcsI RAATTY 3 cut(s) 86, 187, 260
AcuI CTGAAG 2 cut(s) 620, 836
AcyI GRCGYC 2 cut(s) 117, 573
AfaI GTAC 3 cut(s) 643, 774, 837
AfiI CCNNNNNNNGG 5 cut(s) 45, 404, 476, 724, 794
AgsI TTSAA 6 cut(s) 85, 127, 265, 674, 805, 977
AjiI CACGTC 1 cut(s) 1075
AjnI CCWGG 2 cut(s) 103, 457
AloI GAACNNNNNNTCC 2 cut(s) 807, 839
AluBI AGCT 6 cut(s) 668, 820, 856, 896, 950, 1007
AluI AGCT 6 cut(s) 668, 820, 856, 896, 950, 1007
Alw26I GTCTC 4 cut(s) 51, 267, 917, 1023
AlwI GGATC 2 cut(s) 310, 758
AoxI GGCC 2 cut(s) 580, 715
ApeKI GCWGC 3 cut(s) 50, 482, 947
ApoI RAATTY 3 cut(s) 86, 187, 260
Asp700I GAANNNNTTC 1 cut(s) 1017
AspA2I CCTAGG 1 cut(s) 583
AspLEI GCGC 1 cut(s) 482
AsuC2I CCSGG 2 cut(s) 210, 549
AsuHPI GGTGA 1 cut(s) 386
AvrII CCTAGG 1 cut(s) 583
BaeGI GKGCMC 2 cut(s) 216, 740
BalI TGGCCA 1 cut(s) 717
BanI GGYRCC 2 cut(s) 683, 728
BanII GRGCYC 1 cut(s) 624
BauI CACGAG 1 cut(s) 215
BbsI GAAGAC 1 cut(s) 1010
BbvI GCAGC 3 cut(s) 37, 469, 959
BccI CCATC 4 cut(s) 39, 470, 707, 740
BceAI ACGGC 1 cut(s) 716
BcgI CGANNNNNNTGC 2 cut(s) 464, 498
BciT130I CCWGG 2 cut(s) 105, 459
BclI TGATCA 2 cut(s) 636, 699
BcnI CCSGG 2 cut(s) 210, 549
BcoDI GTCTC 4 cut(s) 51, 267, 917, 1023
BfaI CTAG 2 cut(s) 584, 1078
BfmI CTRYAG 1 cut(s) 768
BfoI RGCGCY 1 cut(s) 483
BglII AGATCT 1 cut(s) 157
BisI GCNGC 3 cut(s) 51, 483, 948
BlnI CCTAGG 1 cut(s) 583
BlpI GCTNAGC 1 cut(s) 951
BlsI GCNGC 3 cut(s) 52, 484, 949
Bme1390I CCNGG 4 cut(s) 105, 210, 459, 549
BmgBI CACGTC 1 cut(s) 1075
BmiI GGNNCC 4 cut(s) 102, 394, 685, 730
BmrFI CCNGG 4 cut(s) 105, 210, 459, 549
BmsI GCATC 1 cut(s) 255
BpiI GAAGAC 1 cut(s) 1010
BpmI CTGGAG 1 cut(s) 76
Bpu1102I GCTNAGC 1 cut(s) 951
BpuEI CTTGAG 2 cut(s) 544, 608
BpuMI CCSGG 2 cut(s) 210, 549
BsaHI GRCGYC 2 cut(s) 117, 573
BsaI GGTCTC 2 cut(s) 267, 917
BsaJI CCNNGG 4 cut(s) 209, 583, 679, 732
BsaXI ACNNNNNCTCC 2 cut(s) 264, 294
Bsc4I CCNNNNNNNGG 5 cut(s) 45, 404, 476, 724, 794
Bse1I ACTGG 1 cut(s) 860
Bse3DI GCAATG 1 cut(s) 1067
BseBI CCWGG 2 cut(s) 105, 459
BseDI CCNNGG 4 cut(s) 209, 583, 679, 732
BseGI GGATG 2 cut(s) 50, 751
BseLI CCNNNNNNNGG 5 cut(s) 45, 404, 476, 724, 794
BseMI GCAATG 1 cut(s) 1067
BseMII CTCAG 2 cut(s) 228, 330
BseNI ACTGG 1 cut(s) 860
BseRI GAGGAG 1 cut(s) 477
BseSI GKGCMC 2 cut(s) 216, 740
BseXI GCAGC 3 cut(s) 37, 469, 959
BseYI CCCAGC 1 cut(s) 500
BshFI GGCC 2 cut(s) 582, 717
BshNI GGYRCC 2 cut(s) 683, 728
BsiSI CCGG 2 cut(s) 209, 549
BslI CCNNNNNNNGG 5 cut(s) 45, 404, 476, 724, 794
BsmAI GTCTC 4 cut(s) 51, 267, 917, 1023
BsnI GGCC 2 cut(s) 582, 717
Bso31I GGTCTC 2 cut(s) 267, 917
Bsp1286I GDGCHC 3 cut(s) 216, 624, 740
Bsp143I GATC 5 cut(s) 157, 302, 636, 699, 750
Bsp1720I GCTNAGC 1 cut(s) 951
BspANI GGCC 2 cut(s) 582, 717
BspCNI CTCAG 2 cut(s) 229, 331
BspLI GGNNCC 4 cut(s) 102, 394, 685, 730
BspMAI CTGCAG 1 cut(s) 772
BspPI GGATC 2 cut(s) 310, 758
BspT107I GGYRCC 2 cut(s) 683, 728
BspTNI GGTCTC 2 cut(s) 267, 917
BsrDI GCAATG 1 cut(s) 1067
BsrI ACTGG 1 cut(s) 860
BssECI CCNNGG 4 cut(s) 209, 583, 679, 732
BssMI GATC 5 cut(s) 157, 302, 636, 699, 750
BssNI GRCGYC 2 cut(s) 117, 573
BssSI CACGAG 1 cut(s) 215
BssT1I CCWWGG 2 cut(s) 583, 679
Bst2BI CACGAG 1 cut(s) 215
Bst2UI CCWGG 2 cut(s) 105, 459
Bst4CI ACNGT 3 cut(s) 140, 763, 1043
Bst6I CTCTTC 1 cut(s) 855
BstACI GRCGYC 2 cut(s) 117, 573
BstDEI CTNAG 3 cut(s) 237, 339, 951
BstDSI CCRYGG 1 cut(s) 732
BstF5I GGATG 2 cut(s) 50, 751
BstH2I RGCGCY 1 cut(s) 483
BstHHI GCGC 1 cut(s) 482
BstKTI GATC 5 cut(s) 160, 305, 639, 702, 753
BstMAI GTCTC 4 cut(s) 51, 267, 917, 1023
BstMBI GATC 5 cut(s) 157, 302, 636, 699, 750
BstMWI GCNNNNNNNGC 4 cut(s) 579, 826, 853, 1004
BstNI CCWGG 2 cut(s) 105, 459
BstNSI RCATGY 1 cut(s) 560
BstSCI CCNGG 4 cut(s) 103, 208, 457, 547
BstSFI CTRYAG 1 cut(s) 768
BstSLI GKGCMC 2 cut(s) 216, 740
BstV1I GCAGC 3 cut(s) 37, 469, 959
BstV2I GAAGAC 1 cut(s) 1010
BstX2I RGATCY 2 cut(s) 157, 302
BstXI CCANNNNNNTGG 1 cut(s) 713
BstYI RGATCY 2 cut(s) 157, 302
BsuRI GGCC 2 cut(s) 582, 717
BtgI CCRYGG 1 cut(s) 732
BtrI CACGTC 1 cut(s) 1075
BtsCI GGATG 2 cut(s) 50, 751
BtsIMutI CAGTG 1 cut(s) 759
CfoI GCGC 1 cut(s) 482
CseI GACGC 1 cut(s) 562
Csp6I GTAC 3 cut(s) 642, 773, 836
CviAII CATG 6 cut(s) 166, 278, 386, 424, 557, 877
CviQI GTAC 3 cut(s) 642, 773, 836
DdeI CTNAG 3 cut(s) 237, 339, 951
DpnI GATC 5 cut(s) 159, 304, 638, 701, 752
DpnII GATC 5 cut(s) 157, 302, 636, 699, 750
DrdI GACNNNNNNGTC 1 cut(s) 425
DseDI GACNNNNNNGTC 1 cut(s) 425
EaeI YGGCCR 1 cut(s) 715
Eam1104I CTCTTC 1 cut(s) 855
EarI CTCTTC 1 cut(s) 855
Eco130I CCWWGG 2 cut(s) 583, 679
Eco24I GRGCYC 1 cut(s) 624
Eco31I GGTCTC 2 cut(s) 267, 917
Eco57I CTGAAG 2 cut(s) 620, 836
EcoRII CCWGG 2 cut(s) 103, 457
EcoT14I CCWWGG 2 cut(s) 583, 679
EcoT22I ATGCAT 1 cut(s) 558
EcoT38I GRGCYC 1 cut(s) 624
ErhI CCWWGG 2 cut(s) 583, 679
FaeI CATG 6 cut(s) 169, 281, 389, 427, 560, 880
FalI AAGNNNNNCTT 2 cut(s) 23, 55
FatI CATG 6 cut(s) 165, 277, 385, 423, 556, 876
FauNDI CATATG 1 cut(s) 403
FbaI TGATCA 2 cut(s) 636, 699
FblI GTMKAC 1 cut(s) 466
Fnu4HI GCNGC 3 cut(s) 51, 483, 948
FokI GGATG 2 cut(s) 57, 758
FriOI GRGCYC 1 cut(s) 624
Fsp4HI GCNGC 3 cut(s) 51, 483, 948
FspBI CTAG 2 cut(s) 584, 1078
GlaI GCGC 1 cut(s) 481
GluI GCNGC 3 cut(s) 51, 483, 948
GsaI CCCAGC 1 cut(s) 504
GsuI CTGGAG 1 cut(s) 76
HaeII RGCGCY 1 cut(s) 483
HaeIII GGCC 2 cut(s) 582, 717
HapII CCGG 2 cut(s) 209, 549
HgaI GACGC 1 cut(s) 562
HhaI GCGC 1 cut(s) 482
Hin1I GRCGYC 2 cut(s) 117, 573
Hin1II CATG 6 cut(s) 169, 281, 389, 427, 560, 880
Hin6I GCGC 1 cut(s) 480
HinP1I GCGC 1 cut(s) 480
HindIII AAGCTT 1 cut(s) 818
HinfI GANTC 3 cut(s) 8, 59, 689
HpaII CCGG 2 cut(s) 209, 549
HphI GGTGA 1 cut(s) 386
Hpy166II GTNNAC 2 cut(s) 467, 775
Hpy188I TCNGA 2 cut(s) 418, 536
Hpy188III TCNNGA 4 cut(s) 14, 93, 161, 473
Hpy8I GTNNAC 2 cut(s) 467, 775
Hpy99I CGWCG 3 cut(s) 119, 578, 713
HpyAV CCTTC 3 cut(s) 90, 91, 913
HpyCH4III ACNGT 3 cut(s) 140, 763, 1043
HpyCH4IV ACGT 6 cut(s) 117, 122, 591, 972, 1015, 1074
HpyCH4V TGCA 6 cut(s) 556, 608, 724, 770, 998, 1072
HpyF10VI GCNNNNNNNGC 4 cut(s) 579, 826, 853, 1004
HpyF3I CTNAG 3 cut(s) 237, 339, 951
HpySE526I ACGT 6 cut(s) 117, 122, 591, 972, 1015, 1074
Hsp92I GRCGYC 2 cut(s) 117, 573
Hsp92II CATG 6 cut(s) 169, 281, 389, 427, 560, 880
HspAI GCGC 1 cut(s) 480
Ksp22I TGATCA 2 cut(s) 636, 699
Kzo9I GATC 5 cut(s) 157, 302, 636, 699, 750
LmnI GCTCC 1 cut(s) 490
Lsp1109I GCAGC 3 cut(s) 37, 469, 959
LweI GCATC 1 cut(s) 255
MaeI CTAG 2 cut(s) 584, 1078
MaeII ACGT 6 cut(s) 117, 122, 591, 972, 1015, 1074
MaeIII GTNAC 7 cut(s) 118, 232, 374, 410, 505, 587, 628
MalI GATC 5 cut(s) 159, 304, 638, 701, 752
MboI GATC 5 cut(s) 157, 302, 636, 699, 750
MboII GAAGA 4 cut(s) 664, 667, 842, 1010
MflI RGATCY 2 cut(s) 157, 302
MhlI GDGCHC 3 cut(s) 216, 624, 740
MlsI TGGCCA 1 cut(s) 717
MluCI AATT 5 cut(s) 86, 187, 260, 343, 440
MluNI TGGCCA 1 cut(s) 717
MlyI GAGTC 1 cut(s) 53
MmeI TCCRAC 3 cut(s) 250, 268, 775
Mox20I TGGCCA 1 cut(s) 717
Mph1103I ATGCAT 1 cut(s) 558
MroXI GAANNNNTTC 1 cut(s) 1017
MscI TGGCCA 1 cut(s) 717
MseI TTAA 1 cut(s) 171
MslI CAYNNNNRTG 3 cut(s) 282, 561, 711
Msp20I TGGCCA 1 cut(s) 717
MspI CCGG 2 cut(s) 209, 549
MspR9I CCNGG 4 cut(s) 105, 210, 459, 549
MvaI CCWGG 2 cut(s) 105, 459
MwoI GCNNNNNNNGC 4 cut(s) 579, 826, 853, 1004
NciI CCSGG 2 cut(s) 210, 549
NdeI CATATG 1 cut(s) 403
NdeII GATC 5 cut(s) 157, 302, 636, 699, 750
NlaIII CATG 6 cut(s) 169, 281, 389, 427, 560, 880
NlaIV GGNNCC 4 cut(s) 102, 394, 685, 730
NmuCI GTSAC 4 cut(s) 118, 374, 587, 628
NsiI ATGCAT 1 cut(s) 558
NspI RCATGY 1 cut(s) 560
PdmI GAANNNNTTC 1 cut(s) 1017
PfeI GAWTC 2 cut(s) 8, 689
PflFI GACNNNGTC 1 cut(s) 278
PflMI CCANNNNNTGG 1 cut(s) 404
PkrI GCNGC 3 cut(s) 52, 484, 949
PleI GAGTC 1 cut(s) 53
PpsI GAGTC 1 cut(s) 53
Psp1406I AACGTT 1 cut(s) 972
Psp6I CCWGG 2 cut(s) 103, 457
PspFI CCCAGC 1 cut(s) 500
PspGI CCWGG 2 cut(s) 103, 457
PspN4I GGNNCC 4 cut(s) 102, 394, 685, 730
PstI CTGCAG 1 cut(s) 772
PsuI RGATCY 2 cut(s) 157, 302
PsyI GACNNNGTC 1 cut(s) 278
RsaI GTAC 3 cut(s) 643, 774, 837
RsaNI GTAC 3 cut(s) 642, 773, 836
RseI CAYNNNNRTG 3 cut(s) 282, 561, 711
SaqAI TTAA 1 cut(s) 171
SatI GCNGC 3 cut(s) 51, 483, 948
Sau3AI GATC 5 cut(s) 157, 302, 636, 699, 750
SchI GAGTC 1 cut(s) 53
ScrFI CCNGG 4 cut(s) 105, 210, 459, 549
SduI GDGCHC 3 cut(s) 216, 624, 740
SfaNI GCATC 1 cut(s) 255
SfcI CTRYAG 1 cut(s) 768
SmiMI CAYNNNNRTG 3 cut(s) 282, 561, 711
SmlI CTYRAG 2 cut(s) 523, 623
SmoI CTYRAG 2 cut(s) 523, 623
Sse9I AATT 5 cut(s) 86, 187, 260, 343, 440
SspMI CTAG 2 cut(s) 584, 1078
StyD4I CCNGG 4 cut(s) 103, 208, 457, 547
StyI CCWWGG 2 cut(s) 583, 679
TaaI ACNGT 3 cut(s) 140, 763, 1043
TaiI ACGT 6 cut(s) 120, 125, 594, 975, 1018, 1077
TaqI TCGA 1 cut(s) 474
TasI AATT 5 cut(s) 86, 187, 260, 343, 440
TatI WGTACW 1 cut(s) 772
TfiI GAWTC 2 cut(s) 8, 689
Tru1I TTAA 1 cut(s) 171
Tru9I TTAA 1 cut(s) 171
TscAI CASTG 1 cut(s) 766
TseFI GTSAC 4 cut(s) 118, 374, 587, 628
TseI GCWGC 3 cut(s) 50, 482, 947
Tsp45I GTSAC 4 cut(s) 118, 374, 587, 628
TspDTI ATGAA 2 cut(s) 192, 932
TspGWI ACGGA 2 cut(s) 405, 440
TspRI CASTG 1 cut(s) 766
Tth111I GACNNNGTC 1 cut(s) 278
Van91I CCANNNNNTGG 1 cut(s) 404
XapI RAATTY 3 cut(s) 86, 187, 260
XceI RCATGY 1 cut(s) 560
XcmI CCANNNNNNNNNTGG 1 cut(s) 284
XmaJI CCTAGG 1 cut(s) 583
XmiI GTMKAC 1 cut(s) 466
XmnI GAANNNNTTC 1 cut(s) 1017
XspI CTAG 2 cut(s) 584, 1078
ZraI GACGTC 1 cut(s) 118
Zsp2I ATGCAT 1 cut(s) 558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.