Dehydrogenase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 579, 729 |
| AclWI | GGATC | 2 cut(s) | 310, 758 |
| AcoI | YGGCCR | 1 cut(s) | 168 |
| AcsI | RAATTY | 1 cut(s) | 34 |
| AcvI | CACGTG | 1 cut(s) | 592 |
| AfaI | GTAC | 4 cut(s) | 137, 383, 404, 643 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 45, 166, 476, 794 |
| AgsI | TTSAA | 5 cut(s) | 85, 341, 674, 805, 1072 |
| AjnI | CCWGG | 3 cut(s) | 53, 208, 547 |
| AluBI | AGCT | 4 cut(s) | 668, 856, 968, 1080 |
| AluI | AGCT | 4 cut(s) | 668, 856, 968, 1080 |
| Alw21I | GWGCWC | 1 cut(s) | 838 |
| Alw26I | GTCTC | 3 cut(s) | 267, 520, 917 |
| Alw44I | GTGCAC | 1 cut(s) | 834 |
| AlwI | GGATC | 2 cut(s) | 310, 758 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 668, 1083 |
| AoxI | GGCC | 5 cut(s) | 120, 168, 586, 726, 1074 |
| ApaLI | GTGCAC | 1 cut(s) | 834 |
| ApeKI | GCWGC | 3 cut(s) | 50, 482, 947 |
| ApoI | RAATTY | 1 cut(s) | 34 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1017, 1049 |
| AspLEI | GCGC | 1 cut(s) | 1017 |
| AsuHPI | GGTGA | 2 cut(s) | 119, 326 |
| BaeGI | GKGCMC | 2 cut(s) | 740, 838 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 353, 386 |
| BalI | TGGCCA | 1 cut(s) | 170 |
| BauI | CACGAG | 1 cut(s) | 782 |
| BbrPI | CACGTG | 1 cut(s) | 592 |
| Bbv12I | GWGCWC | 1 cut(s) | 838 |
| BbvI | GCAGC | 3 cut(s) | 37, 469, 959 |
| BccI | CCATC | 5 cut(s) | 39, 383, 470, 740, 820 |
| BciT130I | CCWGG | 3 cut(s) | 55, 210, 549 |
| BclI | TGATCA | 1 cut(s) | 873 |
| BcoDI | GTCTC | 3 cut(s) | 267, 520, 917 |
| BfaI | CTAG | 2 cut(s) | 575, 584 |
| BfoI | RGCGCY | 1 cut(s) | 1018 |
| BisI | GCNGC | 4 cut(s) | 51, 483, 729, 948 |
| BlsI | GCNGC | 4 cut(s) | 52, 484, 730, 949 |
| BmcAI | AGTACT | 1 cut(s) | 383 |
| Bme1390I | CCNGG | 3 cut(s) | 55, 210, 549 |
| BmiI | GGNNCC | 3 cut(s) | 207, 376, 394 |
| BmrFI | CCNGG | 3 cut(s) | 55, 210, 549 |
| BmsI | GCATC | 3 cut(s) | 17, 281, 711 |
| Bpu10I | CCTNAGC | 1 cut(s) | 609 |
| BpuEI | CTTGAG | 3 cut(s) | 76, 544, 872 |
| BsaAI | YACGTR | 2 cut(s) | 369, 592 |
| BsaJI | CCNNGG | 4 cut(s) | 54, 209, 732, 1008 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 264, 294 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 45, 166, 476, 794 |
| Bse1I | ACTGG | 1 cut(s) | 109 |
| BseBI | CCWGG | 3 cut(s) | 55, 210, 549 |
| BseDI | CCNNGG | 4 cut(s) | 54, 209, 732, 1008 |
| BseGI | GGATG | 3 cut(s) | 50, 296, 751 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 45, 166, 476, 794 |
| BseMII | CTCAG | 2 cut(s) | 228, 450 |
| BseNI | ACTGG | 1 cut(s) | 109 |
| BseSI | GKGCMC | 2 cut(s) | 740, 838 |
| BseXI | GCAGC | 3 cut(s) | 37, 469, 959 |
| BsgI | GTGCAG | 1 cut(s) | 275 |
| BshFI | GGCC | 5 cut(s) | 122, 170, 588, 728, 1076 |
| BsiHKAI | GWGCWC | 1 cut(s) | 838 |
| BsiWI | CGTACG | 1 cut(s) | 402 |
| BslFI | GGGAC | 1 cut(s) | 71 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 45, 166, 476, 794 |
| BsmAI | GTCTC | 3 cut(s) | 267, 520, 917 |
| BsmFI | GGGAC | 1 cut(s) | 71 |
| BsmI | GAATGC | 2 cut(s) | 453, 770 |
| BsnI | GGCC | 5 cut(s) | 122, 170, 588, 728, 1076 |
| Bsp1286I | GDGCHC | 2 cut(s) | 740, 838 |
| Bsp143I | GATC | 5 cut(s) | 302, 311, 331, 750, 873 |
| Bsp19I | CCATGG | 2 cut(s) | 732, 1008 |
| BspACI | CCGC | 2 cut(s) | 579, 729 |
| BspANI | GGCC | 5 cut(s) | 122, 170, 588, 728, 1076 |
| BspCNI | CTCAG | 2 cut(s) | 229, 451 |
| BspLI | GGNNCC | 3 cut(s) | 207, 376, 394 |
| BspPI | GGATC | 2 cut(s) | 310, 758 |
| BsrI | ACTGG | 1 cut(s) | 109 |
| BssECI | CCNNGG | 4 cut(s) | 54, 209, 732, 1008 |
| BssMI | GATC | 5 cut(s) | 302, 311, 331, 750, 873 |
| BssSI | CACGAG | 1 cut(s) | 782 |
| BssT1I | CCWWGG | 2 cut(s) | 732, 1008 |
| Bst2BI | CACGAG | 1 cut(s) | 782 |
| Bst2UI | CCWGG | 3 cut(s) | 55, 210, 549 |
| Bst4CI | ACNGT | 3 cut(s) | 140, 634, 763 |
| BstBAI | YACGTR | 2 cut(s) | 369, 592 |
| BstC8I | GCNNGC | 3 cut(s) | 502, 726, 1078 |
| BstDEI | CTNAG | 4 cut(s) | 237, 459, 609, 651 |
| BstDSI | CCRYGG | 2 cut(s) | 732, 1008 |
| BstF5I | GGATG | 3 cut(s) | 50, 296, 751 |
| BstH2I | RGCGCY | 1 cut(s) | 1018 |
| BstHHI | GCGC | 1 cut(s) | 1017 |
| BstKTI | GATC | 5 cut(s) | 305, 314, 334, 753, 876 |
| BstMAI | GTCTC | 3 cut(s) | 267, 520, 917 |
| BstMBI | GATC | 5 cut(s) | 302, 311, 331, 750, 873 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 585, 853 |
| BstNI | CCWGG | 3 cut(s) | 55, 210, 549 |
| BstNSI | RCATGY | 1 cut(s) | 560 |
| BstSCI | CCNGG | 3 cut(s) | 53, 208, 547 |
| BstSLI | GKGCMC | 2 cut(s) | 740, 838 |
| BstV1I | GCAGC | 3 cut(s) | 37, 469, 959 |
| BstX2I | RGATCY | 1 cut(s) | 302 |
| BstYI | RGATCY | 1 cut(s) | 302 |
| BsuRI | GGCC | 5 cut(s) | 122, 170, 588, 728, 1076 |
| BtgI | CCRYGG | 2 cut(s) | 732, 1008 |
| BtsCI | GGATG | 3 cut(s) | 50, 296, 751 |
| BtsI | GCAGTG | 2 cut(s) | 712, 1002 |
| BtsIMutI | CAGTG | 3 cut(s) | 639, 712, 1002 |
| Cac8I | GCNNGC | 3 cut(s) | 502, 726, 1078 |
| CaiI | CAGNNNCTG | 2 cut(s) | 668, 1083 |
| CfoI | GCGC | 1 cut(s) | 1017 |
| CsiI | ACCWGGT | 1 cut(s) | 547 |
| Csp6I | GTAC | 4 cut(s) | 136, 382, 403, 642 |
| CviQI | GTAC | 4 cut(s) | 136, 382, 403, 642 |
| DdeI | CTNAG | 4 cut(s) | 237, 459, 609, 651 |
| DpnI | GATC | 5 cut(s) | 304, 313, 333, 752, 875 |
| DpnII | GATC | 5 cut(s) | 302, 311, 331, 750, 873 |
| DraI | TTTAAA | 1 cut(s) | 127 |
| EaeI | YGGCCR | 1 cut(s) | 168 |
| Eco130I | CCWWGG | 2 cut(s) | 732, 1008 |
| Eco32I | GATATC | 1 cut(s) | 1040 |
| Eco72I | CACGTG | 1 cut(s) | 592 |
| EcoRI | GAATTC | 1 cut(s) | 34 |
| EcoRII | CCWGG | 3 cut(s) | 53, 208, 547 |
| EcoRV | GATATC | 1 cut(s) | 1040 |
| EcoT14I | CCWWGG | 2 cut(s) | 732, 1008 |
| EcoT22I | ATGCAT | 2 cut(s) | 296, 558 |
| ErhI | CCWWGG | 2 cut(s) | 732, 1008 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 23, 55 |
| FaqI | GGGAC | 1 cut(s) | 71 |
| FbaI | TGATCA | 1 cut(s) | 873 |
| Fnu4HI | GCNGC | 4 cut(s) | 51, 483, 729, 948 |
| FokI | GGATG | 3 cut(s) | 57, 303, 758 |
| Fsp4HI | GCNGC | 4 cut(s) | 51, 483, 729, 948 |
| FspBI | CTAG | 2 cut(s) | 575, 584 |
| GlaI | GCGC | 1 cut(s) | 1016 |
| GluI | GCNGC | 4 cut(s) | 51, 483, 729, 948 |
| HaeII | RGCGCY | 1 cut(s) | 1018 |
| HaeIII | GGCC | 5 cut(s) | 122, 170, 588, 728, 1076 |
| HhaI | GCGC | 1 cut(s) | 1017 |
| Hin6I | GCGC | 1 cut(s) | 1015 |
| HinP1I | GCGC | 1 cut(s) | 1015 |
| HinfI | GANTC | 3 cut(s) | 463, 678, 689 |
| HphI | GGTGA | 2 cut(s) | 119, 326 |
| Hpy166II | GTNNAC | 1 cut(s) | 836 |
| Hpy188I | TCNGA | 5 cut(s) | 157, 418, 873, 999, 1037 |
| Hpy188III | TCNNGA | 6 cut(s) | 14, 93, 458, 473, 851, 1083 |
| Hpy8I | GTNNAC | 1 cut(s) | 836 |
| HpyAV | CCTTC | 8 cut(s) | 90, 170, 652, 799, 876, 913, 1028, 1066 |
| HpyCH4III | ACNGT | 3 cut(s) | 140, 634, 763 |
| HpyCH4IV | ACGT | 3 cut(s) | 368, 401, 591 |
| HpyCH4V | TGCA | 7 cut(s) | 256, 294, 556, 724, 770, 836, 947 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 585, 853 |
| HpyF3I | CTNAG | 4 cut(s) | 237, 459, 609, 651 |
| HpySE526I | ACGT | 3 cut(s) | 368, 401, 591 |
| HspAI | GCGC | 1 cut(s) | 1015 |
| Ksp22I | TGATCA | 1 cut(s) | 873 |
| Kzo9I | GATC | 5 cut(s) | 302, 311, 331, 750, 873 |
| LmnI | GCTCC | 3 cut(s) | 490, 1012, 1085 |
| Lsp1109I | GCAGC | 3 cut(s) | 37, 469, 959 |
| LweI | GCATC | 3 cut(s) | 17, 281, 711 |
| MabI | ACCWGGT | 1 cut(s) | 547 |
| MaeI | CTAG | 2 cut(s) | 575, 584 |
| MaeII | ACGT | 3 cut(s) | 368, 401, 591 |
| MaeIII | GTNAC | 4 cut(s) | 232, 410, 628, 707 |
| MalI | GATC | 5 cut(s) | 304, 313, 333, 752, 875 |
| MboI | GATC | 5 cut(s) | 302, 311, 331, 750, 873 |
| MboII | GAAGA | 2 cut(s) | 667, 898 |
| MfeI | CAATTG | 1 cut(s) | 987 |
| MflI | RGATCY | 1 cut(s) | 302 |
| MhlI | GDGCHC | 2 cut(s) | 740, 838 |
| MlsI | TGGCCA | 1 cut(s) | 170 |
| MluCI | AATT | 3 cut(s) | 34, 85, 987 |
| MluNI | TGGCCA | 1 cut(s) | 170 |
| MlyI | GAGTC | 1 cut(s) | 672 |
| MmeI | TCCRAC | 2 cut(s) | 250, 268 |
| MnlI | CCTC | 8 cut(s) | 232, 401, 480, 500, 655, 858, 879, 970 |
| Mox20I | TGGCCA | 1 cut(s) | 170 |
| Mph1103I | ATGCAT | 2 cut(s) | 296, 558 |
| MscI | TGGCCA | 1 cut(s) | 170 |
| MseI | TTAA | 2 cut(s) | 126, 624 |
| MslI | CAYNNNNRTG | 2 cut(s) | 425, 1007 |
| Msp20I | TGGCCA | 1 cut(s) | 170 |
| MspA1I | CMGCKG | 1 cut(s) | 968 |
| MspR9I | CCNGG | 3 cut(s) | 55, 210, 549 |
| MunI | CAATTG | 1 cut(s) | 987 |
| Mva1269I | GAATGC | 2 cut(s) | 453, 770 |
| MvaI | CCWGG | 3 cut(s) | 55, 210, 549 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 585, 853 |
| NcoI | CCATGG | 2 cut(s) | 732, 1008 |
| NdeII | GATC | 5 cut(s) | 302, 311, 331, 750, 873 |
| NlaIV | GGNNCC | 3 cut(s) | 207, 376, 394 |
| NmuCI | GTSAC | 2 cut(s) | 232, 707 |
| NsiI | ATGCAT | 2 cut(s) | 296, 558 |
| NspI | RCATGY | 1 cut(s) | 560 |
| PctI | GAATGC | 2 cut(s) | 453, 770 |
| PfeI | GAWTC | 2 cut(s) | 463, 689 |
| Pfl23II | CGTACG | 1 cut(s) | 402 |
| PkrI | GCNGC | 4 cut(s) | 52, 484, 730, 949 |
| PleI | GAGTC | 1 cut(s) | 672 |
| PmaCI | CACGTG | 1 cut(s) | 592 |
| PmlI | CACGTG | 1 cut(s) | 592 |
| PpsI | GAGTC | 1 cut(s) | 672 |
| Ppu21I | YACGTR | 2 cut(s) | 369, 592 |
| Psp6I | CCWGG | 3 cut(s) | 53, 208, 547 |
| PspCI | CACGTG | 1 cut(s) | 592 |
| PspGI | CCWGG | 3 cut(s) | 53, 208, 547 |
| PspLI | CGTACG | 1 cut(s) | 402 |
| PspN4I | GGNNCC | 3 cut(s) | 207, 376, 394 |
| PstNI | CAGNNNCTG | 2 cut(s) | 668, 1083 |
| PsuI | RGATCY | 1 cut(s) | 302 |
| PvuII | CAGCTG | 1 cut(s) | 968 |
| RsaI | GTAC | 4 cut(s) | 137, 383, 404, 643 |
| RsaNI | GTAC | 4 cut(s) | 136, 382, 403, 642 |
| RseI | CAYNNNNRTG | 2 cut(s) | 425, 1007 |
| SaqAI | TTAA | 2 cut(s) | 126, 624 |
| SatI | GCNGC | 4 cut(s) | 51, 483, 729, 948 |
| Sau3AI | GATC | 5 cut(s) | 302, 311, 331, 750, 873 |
| ScaI | AGTACT | 1 cut(s) | 383 |
| SchI | GAGTC | 1 cut(s) | 672 |
| ScrFI | CCNGG | 3 cut(s) | 55, 210, 549 |
| SduI | GDGCHC | 2 cut(s) | 740, 838 |
| SexAI | ACCWGGT | 1 cut(s) | 547 |
| SfaNI | GCATC | 3 cut(s) | 17, 281, 711 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 425, 1007 |
| SmlI | CTYRAG | 3 cut(s) | 91, 523, 851 |
| SmoI | CTYRAG | 3 cut(s) | 91, 523, 851 |
| Sse9I | AATT | 3 cut(s) | 34, 85, 987 |
| SsiI | CCGC | 2 cut(s) | 579, 729 |
| SspMI | CTAG | 2 cut(s) | 575, 584 |
| StyD4I | CCNGG | 3 cut(s) | 53, 208, 547 |
| StyI | CCWWGG | 2 cut(s) | 732, 1008 |
| TaaI | ACNGT | 3 cut(s) | 140, 634, 763 |
| TaiI | ACGT | 3 cut(s) | 371, 404, 594 |
| TasI | AATT | 3 cut(s) | 34, 85, 987 |
| TatI | WGTACW | 2 cut(s) | 135, 381 |
| TauI | GCSGC | 1 cut(s) | 731 |
| TfiI | GAWTC | 2 cut(s) | 463, 689 |
| Tru1I | TTAA | 2 cut(s) | 126, 624 |
| Tru9I | TTAA | 2 cut(s) | 126, 624 |
| TscAI | CASTG | 3 cut(s) | 639, 712, 1009 |
| TseFI | GTSAC | 2 cut(s) | 232, 707 |
| TseI | GCWGC | 3 cut(s) | 50, 482, 947 |
| Tsp45I | GTSAC | 2 cut(s) | 232, 707 |
| TspDTI | ATGAA | 4 cut(s) | 192, 433, 681, 932 |
| TspGWI | ACGGA | 1 cut(s) | 420 |
| TspRI | CASTG | 3 cut(s) | 639, 712, 1009 |
| VneI | GTGCAC | 1 cut(s) | 834 |
| XapI | RAATTY | 1 cut(s) | 34 |
| XceI | RCATGY | 1 cut(s) | 560 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 739 |
| XspI | CTAG | 2 cut(s) | 575, 584 |
| ZrmI | AGTACT | 1 cut(s) | 383 |
| Zsp2I | ATGCAT | 2 cut(s) | 296, 558 |