Dehydrogenase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 878 |
| AciI | CCGC | 3 cut(s) | 50, 878, 1080 |
| AclWI | GGATC | 1 cut(s) | 512 |
| AcsI | RAATTY | 2 cut(s) | 260, 334 |
| AfaI | GTAC | 5 cut(s) | 137, 229, 255, 383, 643 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 34, 45, 476, 794 |
| AgsI | TTSAA | 5 cut(s) | 85, 127, 805, 1000, 1072 |
| AjiI | CACGTC | 1 cut(s) | 118 |
| AluBI | AGCT | 4 cut(s) | 686, 856, 896, 968 |
| AluI | AGCT | 4 cut(s) | 686, 856, 896, 968 |
| Alw26I | GTCTC | 2 cut(s) | 520, 683 |
| AlwI | GGATC | 1 cut(s) | 512 |
| Ama87I | CYCGRG | 1 cut(s) | 209 |
| AoxI | GGCC | 2 cut(s) | 36, 535 |
| ApeKI | GCWGC | 3 cut(s) | 482, 593, 728 |
| ApoI | RAATTY | 2 cut(s) | 260, 334 |
| AseI | ATTAAT | 1 cut(s) | 672 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 981 |
| AspLEI | GCGC | 1 cut(s) | 500 |
| AspS9I | GGNCC | 1 cut(s) | 157 |
| AsuC2I | CCSGG | 3 cut(s) | 210, 211, 549 |
| AsuHPI | GGTGA | 1 cut(s) | 244 |
| AvaI | CYCGRG | 1 cut(s) | 209 |
| AvaII | GGWCC | 1 cut(s) | 157 |
| BaeGI | GKGCMC | 1 cut(s) | 740 |
| BbvI | GCAGC | 3 cut(s) | 469, 605, 715 |
| BccI | CCATC | 4 cut(s) | 39, 470, 740, 1003 |
| BceAI | ACGGC | 2 cut(s) | 581, 690 |
| BcnI | CCSGG | 3 cut(s) | 210, 211, 549 |
| BcoDI | GTCTC | 2 cut(s) | 520, 683 |
| BfaI | CTAG | 4 cut(s) | 54, 203, 575, 584 |
| BfmI | CTRYAG | 1 cut(s) | 768 |
| BisI | GCNGC | 5 cut(s) | 51, 483, 594, 729, 1080 |
| BlsI | GCNGC | 5 cut(s) | 52, 484, 595, 730, 1081 |
| Bme1390I | CCNGG | 3 cut(s) | 210, 211, 549 |
| Bme18I | GGWCC | 1 cut(s) | 157 |
| BmeT110I | CYCGRG | 1 cut(s) | 209 |
| BmgBI | CACGTC | 1 cut(s) | 118 |
| BmgT120I | GGNCC | 1 cut(s) | 157 |
| BmiI | GGNNCC | 2 cut(s) | 376, 394 |
| BmrFI | CCNGG | 3 cut(s) | 210, 211, 549 |
| BmsI | GCATC | 1 cut(s) | 281 |
| BpuEI | CTTGAG | 2 cut(s) | 76, 544 |
| BpuMI | CCSGG | 3 cut(s) | 210, 211, 549 |
| BsaJI | CCNNGG | 3 cut(s) | 209, 609, 732 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 264, 294 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 34, 45, 476, 794 |
| Bse1I | ACTGG | 2 cut(s) | 109, 876 |
| BseDI | CCNNGG | 3 cut(s) | 209, 609, 732 |
| BseGI | GGATG | 3 cut(s) | 50, 296, 751 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 34, 45, 476, 794 |
| BseNI | ACTGG | 2 cut(s) | 109, 876 |
| BseRI | GAGGAG | 1 cut(s) | 477 |
| BseSI | GKGCMC | 1 cut(s) | 740 |
| BseXI | GCAGC | 3 cut(s) | 469, 605, 715 |
| BseYI | CCCAGC | 1 cut(s) | 500 |
| Bsh1236I | CGCG | 1 cut(s) | 878 |
| BshFI | GGCC | 2 cut(s) | 38, 537 |
| BsiHKCI | CYCGRG | 1 cut(s) | 209 |
| BsiSI | CCGG | 2 cut(s) | 210, 549 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 34, 45, 476, 794 |
| BsmAI | GTCTC | 2 cut(s) | 520, 683 |
| BsmBI | CGTCTC | 1 cut(s) | 683 |
| BsnI | GGCC | 2 cut(s) | 38, 537 |
| BsoBI | CYCGRG | 1 cut(s) | 209 |
| Bsp1286I | GDGCHC | 1 cut(s) | 740 |
| Bsp143I | GATC | 2 cut(s) | 504, 715 |
| Bsp19I | CCATGG | 1 cut(s) | 732 |
| BspACI | CCGC | 3 cut(s) | 50, 878, 1080 |
| BspANI | GGCC | 2 cut(s) | 38, 537 |
| BspFNI | CGCG | 1 cut(s) | 878 |
| BspLI | GGNNCC | 2 cut(s) | 376, 394 |
| BspMAI | CTGCAG | 1 cut(s) | 772 |
| BspPI | GGATC | 1 cut(s) | 512 |
| BsrI | ACTGG | 2 cut(s) | 109, 876 |
| BssECI | CCNNGG | 3 cut(s) | 209, 609, 732 |
| BssMI | GATC | 2 cut(s) | 504, 715 |
| BssT1I | CCWWGG | 2 cut(s) | 609, 732 |
| Bst4CI | ACNGT | 4 cut(s) | 140, 232, 634, 763 |
| BstC8I | GCNNGC | 3 cut(s) | 726, 949, 953 |
| BstDSI | CCRYGG | 1 cut(s) | 732 |
| BstF5I | GGATG | 3 cut(s) | 50, 296, 751 |
| BstFNI | CGCG | 1 cut(s) | 878 |
| BstHHI | GCGC | 1 cut(s) | 500 |
| BstKTI | GATC | 2 cut(s) | 507, 718 |
| BstMAI | GTCTC | 2 cut(s) | 520, 683 |
| BstMBI | GATC | 2 cut(s) | 504, 715 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 776, 853 |
| BstNSI | RCATGY | 1 cut(s) | 593 |
| BstSCI | CCNGG | 3 cut(s) | 208, 209, 547 |
| BstSFI | CTRYAG | 1 cut(s) | 768 |
| BstSLI | GKGCMC | 1 cut(s) | 740 |
| BstUI | CGCG | 1 cut(s) | 878 |
| BstV1I | GCAGC | 3 cut(s) | 469, 605, 715 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 337 |
| BsuRI | GGCC | 2 cut(s) | 38, 537 |
| BtgI | CCRYGG | 1 cut(s) | 732 |
| BtgZI | GCGATG | 1 cut(s) | 1022 |
| BtrI | CACGTC | 1 cut(s) | 118 |
| BtsCI | GGATG | 3 cut(s) | 50, 296, 751 |
| BtsIMutI | CAGTG | 2 cut(s) | 639, 869 |
| Cac8I | GCNNGC | 3 cut(s) | 726, 949, 953 |
| CfoI | GCGC | 1 cut(s) | 500 |
| Cfr13I | GGNCC | 1 cut(s) | 157 |
| Cfr9I | CCCGGG | 1 cut(s) | 209 |
| CseI | GACGC | 1 cut(s) | 1004 |
| Csp6I | GTAC | 5 cut(s) | 136, 228, 254, 382, 642 |
| CviAII | CATG | 7 cut(s) | 215, 397, 404, 424, 533, 590, 733 |
| CviQI | GTAC | 5 cut(s) | 136, 228, 254, 382, 642 |
| DpnI | GATC | 2 cut(s) | 506, 717 |
| DpnII | GATC | 2 cut(s) | 504, 715 |
| DraI | TTTAAA | 2 cut(s) | 265, 918 |
| Eco130I | CCWWGG | 2 cut(s) | 609, 732 |
| Eco147I | AGGCCT | 1 cut(s) | 38 |
| Eco47I | GGWCC | 1 cut(s) | 157 |
| Eco88I | CYCGRG | 1 cut(s) | 209 |
| EcoT14I | CCWWGG | 2 cut(s) | 609, 732 |
| EcoT22I | ATGCAT | 1 cut(s) | 558 |
| ErhI | CCWWGG | 2 cut(s) | 609, 732 |
| Esp3I | CGTCTC | 1 cut(s) | 683 |
| FaeI | CATG | 7 cut(s) | 218, 400, 407, 427, 536, 593, 736 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 23, 55 |
| FatI | CATG | 7 cut(s) | 214, 396, 403, 423, 532, 589, 732 |
| FauI | CCCGC | 1 cut(s) | 871 |
| Fnu4HI | GCNGC | 5 cut(s) | 51, 483, 594, 729, 1080 |
| FokI | GGATG | 3 cut(s) | 57, 303, 758 |
| Fsp4HI | GCNGC | 5 cut(s) | 51, 483, 594, 729, 1080 |
| FspBI | CTAG | 4 cut(s) | 54, 203, 575, 584 |
| GlaI | GCGC | 1 cut(s) | 499 |
| GluI | GCNGC | 5 cut(s) | 51, 483, 594, 729, 1080 |
| GsaI | CCCAGC | 1 cut(s) | 504 |
| HaeIII | GGCC | 2 cut(s) | 38, 537 |
| HapII | CCGG | 2 cut(s) | 210, 549 |
| HgaI | GACGC | 1 cut(s) | 1004 |
| HhaI | GCGC | 1 cut(s) | 500 |
| Hin1II | CATG | 7 cut(s) | 218, 400, 407, 427, 536, 593, 736 |
| Hin6I | GCGC | 1 cut(s) | 498 |
| HinP1I | GCGC | 1 cut(s) | 498 |
| HinfI | GANTC | 2 cut(s) | 59, 689 |
| HpaII | CCGG | 2 cut(s) | 210, 549 |
| HphI | GGTGA | 1 cut(s) | 244 |
| Hpy166II | GTNNAC | 3 cut(s) | 710, 775, 836 |
| Hpy188I | TCNGA | 2 cut(s) | 157, 418 |
| Hpy188III | TCNNGA | 5 cut(s) | 14, 93, 172, 458, 473 |
| Hpy8I | GTNNAC | 3 cut(s) | 710, 775, 836 |
| Hpy99I | CGWCG | 1 cut(s) | 392 |
| HpyAV | CCTTC | 6 cut(s) | 28, 90, 91, 106, 170, 652 |
| HpyCH4III | ACNGT | 4 cut(s) | 140, 232, 634, 763 |
| HpyCH4IV | ACGT | 1 cut(s) | 117 |
| HpyCH4V | TGCA | 6 cut(s) | 556, 593, 724, 770, 906, 947 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 776, 853 |
| HpySE526I | ACGT | 1 cut(s) | 117 |
| Hsp92II | CATG | 7 cut(s) | 218, 400, 407, 427, 536, 593, 736 |
| HspAI | GCGC | 1 cut(s) | 498 |
| Kzo9I | GATC | 2 cut(s) | 504, 715 |
| LmnI | GCTCC | 2 cut(s) | 490, 1012 |
| Lsp1109I | GCAGC | 3 cut(s) | 469, 605, 715 |
| LweI | GCATC | 1 cut(s) | 281 |
| MaeI | CTAG | 4 cut(s) | 54, 203, 575, 584 |
| MaeII | ACGT | 1 cut(s) | 117 |
| MaeIII | GTNAC | 3 cut(s) | 118, 232, 628 |
| MalI | GATC | 2 cut(s) | 506, 717 |
| MboI | GATC | 2 cut(s) | 504, 715 |
| MboII | GAAGA | 3 cut(s) | 43, 674, 898 |
| MfeI | CAATTG | 1 cut(s) | 987 |
| MhlI | GDGCHC | 1 cut(s) | 740 |
| MluCI | AATT | 5 cut(s) | 260, 322, 334, 861, 987 |
| MmeI | TCCRAC | 2 cut(s) | 250, 268 |
| MnlI | CCTC | 6 cut(s) | 210, 480, 498, 655, 875, 905 |
| Mph1103I | ATGCAT | 1 cut(s) | 558 |
| MroXI | GAANNNNTTC | 1 cut(s) | 981 |
| MseI | TTAA | 5 cut(s) | 264, 624, 672, 917, 1084 |
| MslI | CAYNNNNRTG | 2 cut(s) | 425, 561 |
| MspA1I | CMGCKG | 1 cut(s) | 968 |
| MspI | CCGG | 2 cut(s) | 210, 549 |
| MspR9I | CCNGG | 3 cut(s) | 210, 211, 549 |
| MunI | CAATTG | 1 cut(s) | 987 |
| MvnI | CGCG | 1 cut(s) | 878 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 776, 853 |
| NciI | CCSGG | 3 cut(s) | 210, 211, 549 |
| NcoI | CCATGG | 1 cut(s) | 732 |
| NdeII | GATC | 2 cut(s) | 504, 715 |
| NlaIII | CATG | 7 cut(s) | 218, 400, 407, 427, 536, 593, 736 |
| NlaIV | GGNNCC | 2 cut(s) | 376, 394 |
| NmuCI | GTSAC | 3 cut(s) | 118, 232, 628 |
| NsiI | ATGCAT | 1 cut(s) | 558 |
| NspI | RCATGY | 1 cut(s) | 593 |
| PceI | AGGCCT | 1 cut(s) | 38 |
| PdmI | GAANNNNTTC | 1 cut(s) | 981 |
| PfeI | GAWTC | 2 cut(s) | 59, 689 |
| PkrI | GCNGC | 5 cut(s) | 52, 484, 595, 730, 1081 |
| PshBI | ATTAAT | 1 cut(s) | 672 |
| PspFI | CCCAGC | 1 cut(s) | 500 |
| PspN4I | GGNNCC | 2 cut(s) | 376, 394 |
| PspPI | GGNCC | 1 cut(s) | 157 |
| PstI | CTGCAG | 1 cut(s) | 772 |
| PvuII | CAGCTG | 1 cut(s) | 968 |
| RsaI | GTAC | 5 cut(s) | 137, 229, 255, 383, 643 |
| RsaNI | GTAC | 5 cut(s) | 136, 228, 254, 382, 642 |
| RseI | CAYNNNNRTG | 2 cut(s) | 425, 561 |
| SaqAI | TTAA | 5 cut(s) | 264, 624, 672, 917, 1084 |
| SatI | GCNGC | 5 cut(s) | 51, 483, 594, 729, 1080 |
| Sau3AI | GATC | 2 cut(s) | 504, 715 |
| Sau96I | GGNCC | 1 cut(s) | 157 |
| ScrFI | CCNGG | 3 cut(s) | 210, 211, 549 |
| SduI | GDGCHC | 1 cut(s) | 740 |
| SfaNI | GCATC | 1 cut(s) | 281 |
| SfcI | CTRYAG | 1 cut(s) | 768 |
| SinI | GGWCC | 1 cut(s) | 157 |
| SmaI | CCCGGG | 1 cut(s) | 211 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 425, 561 |
| SmlI | CTYRAG | 2 cut(s) | 91, 523 |
| SmoI | CTYRAG | 2 cut(s) | 91, 523 |
| Sse9I | AATT | 5 cut(s) | 260, 322, 334, 861, 987 |
| SseBI | AGGCCT | 1 cut(s) | 38 |
| SsiI | CCGC | 3 cut(s) | 50, 878, 1080 |
| SspMI | CTAG | 4 cut(s) | 54, 203, 575, 584 |
| StuI | AGGCCT | 1 cut(s) | 38 |
| StyD4I | CCNGG | 3 cut(s) | 208, 209, 547 |
| StyI | CCWWGG | 2 cut(s) | 609, 732 |
| TaaI | ACNGT | 4 cut(s) | 140, 232, 634, 763 |
| TaiI | ACGT | 1 cut(s) | 120 |
| TaqI | TCGA | 2 cut(s) | 975, 1053 |
| TasI | AATT | 5 cut(s) | 260, 322, 334, 861, 987 |
| TatI | WGTACW | 2 cut(s) | 135, 253 |
| TauI | GCSGC | 2 cut(s) | 53, 1082 |
| TfiI | GAWTC | 2 cut(s) | 59, 689 |
| Tru1I | TTAA | 5 cut(s) | 264, 624, 672, 917, 1084 |
| Tru9I | TTAA | 5 cut(s) | 264, 624, 672, 917, 1084 |
| TscAI | CASTG | 2 cut(s) | 639, 876 |
| TseFI | GTSAC | 3 cut(s) | 118, 232, 628 |
| TseI | GCWGC | 3 cut(s) | 482, 593, 728 |
| Tsp45I | GTSAC | 3 cut(s) | 118, 232, 628 |
| TspDTI | ATGAA | 4 cut(s) | 433, 450, 521, 681 |
| TspGWI | ACGGA | 2 cut(s) | 405, 440 |
| TspMI | CCCGGG | 1 cut(s) | 209 |
| TspRI | CASTG | 2 cut(s) | 639, 876 |
| VpaK11BI | GGWCC | 1 cut(s) | 157 |
| VspI | ATTAAT | 1 cut(s) | 672 |
| XapI | RAATTY | 2 cut(s) | 260, 334 |
| XceI | RCATGY | 1 cut(s) | 593 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 739 |
| XmaI | CCCGGG | 1 cut(s) | 209 |
| XmnI | GAANNNNTTC | 1 cut(s) | 981 |
| XspI | CTAG | 4 cut(s) | 54, 203, 575, 584 |
| Zsp2I | ATGCAT | 1 cut(s) | 558 |