Dehydrogenase
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 20 |
| Acc65I | GGTACC | 1 cut(s) | 773 |
| AccB1I | GGYRCC | 1 cut(s) | 773 |
| AciI | CCGC | 3 cut(s) | 119, 415, 514 |
| AclWI | GGATC | 1 cut(s) | 794 |
| AcoI | YGGCCR | 1 cut(s) | 492 |
| AcsI | RAATTY | 1 cut(s) | 335 |
| AfaI | GTAC | 5 cut(s) | 330, 391, 446, 706, 775 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 119, 718, 783, 830 |
| AgsI | TTSAA | 3 cut(s) | 340, 806, 841 |
| AhlI | ACTAGT | 1 cut(s) | 181 |
| AjiI | CACGTC | 1 cut(s) | 655 |
| AjnI | CCWGG | 1 cut(s) | 584 |
| AluBI | AGCT | 4 cut(s) | 731, 856, 892, 922 |
| AluI | AGCT | 4 cut(s) | 731, 856, 892, 922 |
| Alw21I | GWGCWC | 1 cut(s) | 874 |
| Alw26I | GTCTC | 2 cut(s) | 190, 225 |
| Alw44I | GTGCAC | 1 cut(s) | 870 |
| AlwI | GGATC | 1 cut(s) | 794 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 585 |
| Ama87I | CYCGRG | 1 cut(s) | 284 |
| AoxI | GGCC | 3 cut(s) | 492, 634, 649 |
| ApaLI | GTGCAC | 1 cut(s) | 870 |
| ApeKI | GCWGC | 2 cut(s) | 545, 764 |
| ApoI | RAATTY | 1 cut(s) | 335 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 785, 817 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 173 |
| Asp718I | GGTACC | 1 cut(s) | 773 |
| AspA2I | CCTAGG | 2 cut(s) | 637, 742 |
| AspLEI | GCGC | 1 cut(s) | 440 |
| AspS9I | GGNCC | 1 cut(s) | 9 |
| AsuC2I | CCSGG | 5 cut(s) | 145, 195, 285, 286, 565 |
| AsuHPI | GGTGA | 2 cut(s) | 118, 697 |
| AvaI | CYCGRG | 1 cut(s) | 284 |
| AvaII | GGWCC | 1 cut(s) | 9 |
| AvrII | CCTAGG | 2 cut(s) | 637, 742 |
| BaeGI | GKGCMC | 1 cut(s) | 874 |
| BanI | GGYRCC | 1 cut(s) | 773 |
| BanII | GRGCYC | 1 cut(s) | 195 |
| Bbv12I | GWGCWC | 1 cut(s) | 874 |
| BbvI | GCAGC | 2 cut(s) | 532, 751 |
| BccI | CCATC | 2 cut(s) | 26, 776 |
| BceAI | ACGGC | 1 cut(s) | 74 |
| BciT130I | CCWGG | 1 cut(s) | 586 |
| BclI | TGATCA | 1 cut(s) | 699 |
| BcnI | CCSGG | 5 cut(s) | 145, 195, 285, 286, 565 |
| BcoDI | GTCTC | 2 cut(s) | 190, 225 |
| BcuI | ACTAGT | 1 cut(s) | 181 |
| BfaI | CTAG | 4 cut(s) | 182, 612, 638, 743 |
| BfmI | CTRYAG | 2 cut(s) | 466, 577 |
| BfoI | RGCGCY | 1 cut(s) | 441 |
| BfuAI | ACCTGC | 1 cut(s) | 20 |
| BisI | GCNGC | 3 cut(s) | 415, 546, 765 |
| BlnI | CCTAGG | 2 cut(s) | 637, 742 |
| BlsI | GCNGC | 3 cut(s) | 416, 547, 766 |
| BmcAI | AGTACT | 1 cut(s) | 446 |
| Bme1390I | CCNGG | 6 cut(s) | 145, 195, 285, 286, 565, 586 |
| Bme18I | GGWCC | 1 cut(s) | 9 |
| BmeT110I | CYCGRG | 1 cut(s) | 284 |
| BmgBI | CACGTC | 1 cut(s) | 655 |
| BmgT120I | GGNCC | 1 cut(s) | 9 |
| BmiI | GGNNCC | 3 cut(s) | 282, 457, 775 |
| BmrFI | CCNGG | 6 cut(s) | 145, 195, 285, 286, 565, 586 |
| BmsI | GCATC | 2 cut(s) | 344, 525 |
| BpmI | CTGGAG | 1 cut(s) | 607 |
| BpuEI | CTTGAG | 3 cut(s) | 230, 422, 908 |
| BpuMI | CCSGG | 5 cut(s) | 145, 195, 285, 286, 565 |
| BsaI | GGTCTC | 1 cut(s) | 225 |
| BsaJI | CCNNGG | 6 cut(s) | 284, 584, 637, 672, 742, 777 |
| BsaWI | WCCGGW | 1 cut(s) | 895 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 119, 718, 783, 830 |
| Bse1I | ACTGG | 1 cut(s) | 875 |
| Bse3DI | GCAATG | 3 cut(s) | 22, 331, 666 |
| BseBI | CCWGG | 1 cut(s) | 586 |
| BseDI | CCNNGG | 6 cut(s) | 284, 584, 637, 672, 742, 777 |
| BseGI | GGATG | 4 cut(s) | 359, 516, 787, 811 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 119, 718, 783, 830 |
| BseMI | GCAATG | 3 cut(s) | 22, 331, 666 |
| BseMII | CTCAG | 1 cut(s) | 180 |
| BseNI | ACTGG | 1 cut(s) | 875 |
| BseRI | GAGGAG | 1 cut(s) | 737 |
| BseSI | GKGCMC | 1 cut(s) | 874 |
| BseXI | GCAGC | 2 cut(s) | 532, 751 |
| BshFI | GGCC | 3 cut(s) | 494, 636, 651 |
| BshNI | GGYRCC | 1 cut(s) | 773 |
| BsiHKAI | GWGCWC | 1 cut(s) | 874 |
| BsiHKCI | CYCGRG | 1 cut(s) | 284 |
| BsiSI | CCGG | 5 cut(s) | 144, 195, 285, 564, 896 |
| BslFI | GGGAC | 2 cut(s) | 147, 694 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 119, 718, 783, 830 |
| BsmAI | GTCTC | 2 cut(s) | 190, 225 |
| BsmFI | GGGAC | 2 cut(s) | 147, 694 |
| BsnI | GGCC | 3 cut(s) | 494, 636, 651 |
| Bso31I | GGTCTC | 1 cut(s) | 225 |
| BsoBI | CYCGRG | 1 cut(s) | 284 |
| Bsp1286I | GDGCHC | 2 cut(s) | 195, 874 |
| Bsp1407I | TGTACA | 1 cut(s) | 328 |
| Bsp143I | GATC | 3 cut(s) | 374, 699, 786 |
| Bsp19I | CCATGG | 1 cut(s) | 777 |
| BspACI | CCGC | 3 cut(s) | 119, 415, 514 |
| BspANI | GGCC | 3 cut(s) | 494, 636, 651 |
| BspCNI | CTCAG | 1 cut(s) | 181 |
| BspLI | GGNNCC | 3 cut(s) | 282, 457, 775 |
| BspMI | ACCTGC | 1 cut(s) | 20 |
| BspPI | GGATC | 1 cut(s) | 794 |
| BspT107I | GGYRCC | 1 cut(s) | 773 |
| BspTNI | GGTCTC | 1 cut(s) | 225 |
| BsrDI | GCAATG | 3 cut(s) | 22, 331, 666 |
| BsrGI | TGTACA | 1 cut(s) | 328 |
| BsrI | ACTGG | 1 cut(s) | 875 |
| BssECI | CCNNGG | 6 cut(s) | 284, 584, 637, 672, 742, 777 |
| BssMI | GATC | 3 cut(s) | 374, 699, 786 |
| BssT1I | CCWWGG | 4 cut(s) | 637, 672, 742, 777 |
| Bst2UI | CCWGG | 1 cut(s) | 586 |
| Bst4CI | ACNGT | 3 cut(s) | 154, 697, 799 |
| BstAUI | TGTACA | 1 cut(s) | 328 |
| BstC8I | GCNNGC | 1 cut(s) | 617 |
| BstDEI | CTNAG | 1 cut(s) | 189 |
| BstDSI | CCRYGG | 1 cut(s) | 777 |
| BstF5I | GGATG | 4 cut(s) | 359, 516, 787, 811 |
| BstH2I | RGCGCY | 1 cut(s) | 441 |
| BstHHI | GCGC | 1 cut(s) | 440 |
| BstKTI | GATC | 3 cut(s) | 377, 702, 789 |
| BstMAI | GTCTC | 2 cut(s) | 190, 225 |
| BstMBI | GATC | 3 cut(s) | 374, 699, 786 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 616, 648, 889 |
| BstNI | CCWGG | 1 cut(s) | 586 |
| BstNSI | RCATGY | 2 cut(s) | 244, 619 |
| BstSCI | CCNGG | 6 cut(s) | 143, 193, 283, 284, 563, 584 |
| BstSFI | CTRYAG | 2 cut(s) | 466, 577 |
| BstSLI | GKGCMC | 1 cut(s) | 874 |
| BstV1I | GCAGC | 2 cut(s) | 532, 751 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 679 |
| BsuRI | GGCC | 3 cut(s) | 494, 636, 651 |
| BtgI | CCRYGG | 1 cut(s) | 777 |
| BtrI | CACGTC | 1 cut(s) | 655 |
| BtsCI | GGATG | 4 cut(s) | 359, 516, 787, 811 |
| BtsIMutI | CAGTG | 2 cut(s) | 307, 702 |
| BveI | ACCTGC | 1 cut(s) | 20 |
| Cac8I | GCNNGC | 1 cut(s) | 617 |
| CaiI | CAGNNNCTG | 1 cut(s) | 585 |
| CfoI | GCGC | 1 cut(s) | 440 |
| Cfr13I | GGNCC | 1 cut(s) | 9 |
| Cfr9I | CCCGGG | 1 cut(s) | 284 |
| Csp6I | GTAC | 5 cut(s) | 329, 390, 445, 705, 774 |
| CviAII | CATG | 7 cut(s) | 6, 241, 290, 616, 778, 848, 913 |
| CviQI | GTAC | 5 cut(s) | 329, 390, 445, 705, 774 |
| DdeI | CTNAG | 1 cut(s) | 189 |
| DpnI | GATC | 3 cut(s) | 376, 701, 788 |
| DpnII | GATC | 3 cut(s) | 374, 699, 786 |
| EaeI | YGGCCR | 1 cut(s) | 492 |
| Eco130I | CCWWGG | 4 cut(s) | 637, 672, 742, 777 |
| Eco24I | GRGCYC | 1 cut(s) | 195 |
| Eco31I | GGTCTC | 1 cut(s) | 225 |
| Eco47I | GGWCC | 1 cut(s) | 9 |
| Eco88I | CYCGRG | 1 cut(s) | 284 |
| EcoRII | CCWGG | 1 cut(s) | 584 |
| EcoT14I | CCWWGG | 4 cut(s) | 637, 672, 742, 777 |
| EcoT22I | ATGCAT | 2 cut(s) | 359, 621 |
| EcoT38I | GRGCYC | 1 cut(s) | 195 |
| ErhI | CCWWGG | 4 cut(s) | 637, 672, 742, 777 |
| FaeI | CATG | 7 cut(s) | 9, 244, 293, 619, 781, 851, 916 |
| FaqI | GGGAC | 2 cut(s) | 147, 694 |
| FatI | CATG | 7 cut(s) | 5, 240, 289, 615, 777, 847, 912 |
| FauI | CCCGC | 1 cut(s) | 112 |
| FauNDI | CATATG | 1 cut(s) | 621 |
| FbaI | TGATCA | 1 cut(s) | 699 |
| Fnu4HI | GCNGC | 3 cut(s) | 415, 546, 765 |
| FokI | GGATG | 4 cut(s) | 366, 503, 794, 798 |
| FriOI | GRGCYC | 1 cut(s) | 195 |
| Fsp4HI | GCNGC | 3 cut(s) | 415, 546, 765 |
| FspBI | CTAG | 4 cut(s) | 182, 612, 638, 743 |
| GlaI | GCGC | 1 cut(s) | 439 |
| GluI | GCNGC | 3 cut(s) | 415, 546, 765 |
| GsuI | CTGGAG | 1 cut(s) | 607 |
| HaeII | RGCGCY | 1 cut(s) | 441 |
| HaeIII | GGCC | 3 cut(s) | 494, 636, 651 |
| HapII | CCGG | 5 cut(s) | 144, 195, 285, 564, 896 |
| HhaI | GCGC | 1 cut(s) | 440 |
| Hin1II | CATG | 7 cut(s) | 9, 244, 293, 619, 781, 851, 916 |
| Hin6I | GCGC | 1 cut(s) | 438 |
| HinP1I | GCGC | 1 cut(s) | 438 |
| HindIII | AAGCTT | 1 cut(s) | 854 |
| HpaII | CCGG | 5 cut(s) | 144, 195, 285, 564, 896 |
| HphI | GGTGA | 2 cut(s) | 118, 697 |
| Hpy166II | GTNNAC | 1 cut(s) | 872 |
| Hpy188I | TCNGA | 4 cut(s) | 61, 179, 190, 481 |
| Hpy188III | TCNNGA | 2 cut(s) | 247, 887 |
| Hpy8I | GTNNAC | 1 cut(s) | 872 |
| Hpy99I | CGWCG | 2 cut(s) | 455, 659 |
| HpyAV | CCTTC | 2 cut(s) | 245, 682 |
| HpyCH4III | ACNGT | 3 cut(s) | 154, 697, 799 |
| HpyCH4IV | ACGT | 2 cut(s) | 654, 707 |
| HpyCH4V | TGCA | 4 cut(s) | 15, 357, 619, 872 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 616, 648, 889 |
| HpyF3I | CTNAG | 1 cut(s) | 189 |
| HpySE526I | ACGT | 2 cut(s) | 654, 707 |
| Hsp92II | CATG | 7 cut(s) | 9, 244, 293, 619, 781, 851, 916 |
| HspAI | GCGC | 1 cut(s) | 438 |
| KpnI | GGTACC | 1 cut(s) | 777 |
| Ksp22I | TGATCA | 1 cut(s) | 699 |
| Kzo9I | GATC | 3 cut(s) | 374, 699, 786 |
| LmnI | GCTCC | 3 cut(s) | 321, 553, 746 |
| Lsp1109I | GCAGC | 2 cut(s) | 532, 751 |
| LweI | GCATC | 2 cut(s) | 344, 525 |
| MaeI | CTAG | 4 cut(s) | 182, 612, 638, 743 |
| MaeII | ACGT | 2 cut(s) | 654, 707 |
| MaeIII | GTNAC | 4 cut(s) | 124, 307, 473, 691 |
| MalI | GATC | 3 cut(s) | 376, 701, 788 |
| MboI | GATC | 3 cut(s) | 374, 699, 786 |
| MboII | GAAGA | 1 cut(s) | 41 |
| MhlI | GDGCHC | 2 cut(s) | 195, 874 |
| MluCI | AATT | 1 cut(s) | 335 |
| MmeI | TCCRAC | 1 cut(s) | 331 |
| MnlI | CCTC | 3 cut(s) | 715, 718, 894 |
| Mph1103I | ATGCAT | 2 cut(s) | 359, 621 |
| MroXI | GAANNNNTTC | 1 cut(s) | 173 |
| MseI | TTAA | 1 cut(s) | 735 |
| MslI | CAYNNNNRTG | 3 cut(s) | 556, 620, 624 |
| MspI | CCGG | 5 cut(s) | 144, 195, 285, 564, 896 |
| MspR9I | CCNGG | 6 cut(s) | 145, 195, 285, 286, 565, 586 |
| MvaI | CCWGG | 1 cut(s) | 586 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 616, 648, 889 |
| NciI | CCSGG | 5 cut(s) | 145, 195, 285, 286, 565 |
| NcoI | CCATGG | 1 cut(s) | 777 |
| NdeI | CATATG | 1 cut(s) | 621 |
| NdeII | GATC | 3 cut(s) | 374, 699, 786 |
| NlaIII | CATG | 7 cut(s) | 9, 244, 293, 619, 781, 851, 916 |
| NlaIV | GGNNCC | 3 cut(s) | 282, 457, 775 |
| NmuCI | GTSAC | 2 cut(s) | 124, 307 |
| NsiI | ATGCAT | 2 cut(s) | 359, 621 |
| NspI | RCATGY | 2 cut(s) | 244, 619 |
| PaeI | GCATGC | 1 cut(s) | 619 |
| PdmI | GAANNNNTTC | 1 cut(s) | 173 |
| PflFI | GACNNNGTC | 1 cut(s) | 296 |
| PkrI | GCNGC | 3 cut(s) | 416, 547, 766 |
| Psp6I | CCWGG | 1 cut(s) | 584 |
| PspGI | CCWGG | 1 cut(s) | 584 |
| PspN4I | GGNNCC | 3 cut(s) | 282, 457, 775 |
| PspPI | GGNCC | 1 cut(s) | 9 |
| PstNI | CAGNNNCTG | 1 cut(s) | 585 |
| PsyI | GACNNNGTC | 1 cut(s) | 296 |
| RsaI | GTAC | 5 cut(s) | 330, 391, 446, 706, 775 |
| RsaNI | GTAC | 5 cut(s) | 329, 390, 445, 705, 774 |
| RseI | CAYNNNNRTG | 3 cut(s) | 556, 620, 624 |
| SaqAI | TTAA | 1 cut(s) | 735 |
| SatI | GCNGC | 3 cut(s) | 415, 546, 765 |
| Sau3AI | GATC | 3 cut(s) | 374, 699, 786 |
| Sau96I | GGNCC | 1 cut(s) | 9 |
| ScaI | AGTACT | 1 cut(s) | 446 |
| ScrFI | CCNGG | 6 cut(s) | 145, 195, 285, 286, 565, 586 |
| SduI | GDGCHC | 2 cut(s) | 195, 874 |
| SfaNI | GCATC | 2 cut(s) | 344, 525 |
| SfcI | CTRYAG | 2 cut(s) | 466, 577 |
| SinI | GGWCC | 1 cut(s) | 9 |
| SmaI | CCCGGG | 1 cut(s) | 286 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 556, 620, 624 |
| SmlI | CTYRAG | 3 cut(s) | 245, 401, 887 |
| SmoI | CTYRAG | 3 cut(s) | 245, 401, 887 |
| SpeI | ACTAGT | 1 cut(s) | 181 |
| SphI | GCATGC | 1 cut(s) | 619 |
| Sse9I | AATT | 1 cut(s) | 335 |
| SsiI | CCGC | 3 cut(s) | 119, 415, 514 |
| SspI | AATATT | 2 cut(s) | 173, 209 |
| SspMI | CTAG | 4 cut(s) | 182, 612, 638, 743 |
| StyD4I | CCNGG | 6 cut(s) | 143, 193, 283, 284, 563, 584 |
| StyI | CCWWGG | 4 cut(s) | 637, 672, 742, 777 |
| TaaI | ACNGT | 3 cut(s) | 154, 697, 799 |
| TaiI | ACGT | 2 cut(s) | 657, 710 |
| TasI | AATT | 1 cut(s) | 335 |
| TatI | WGTACW | 2 cut(s) | 328, 444 |
| TauI | GCSGC | 1 cut(s) | 417 |
| Tru1I | TTAA | 1 cut(s) | 735 |
| Tru9I | TTAA | 1 cut(s) | 735 |
| TscAI | CASTG | 2 cut(s) | 307, 702 |
| TseFI | GTSAC | 2 cut(s) | 124, 307 |
| TseI | GCWGC | 2 cut(s) | 545, 764 |
| Tsp45I | GTSAC | 2 cut(s) | 124, 307 |
| TspDTI | ATGAA | 5 cut(s) | 42, 58, 496, 513, 677 |
| TspGWI | ACGGA | 1 cut(s) | 468 |
| TspMI | CCCGGG | 1 cut(s) | 284 |
| TspRI | CASTG | 2 cut(s) | 307, 702 |
| Tth111I | GACNNNGTC | 1 cut(s) | 296 |
| VneI | GTGCAC | 1 cut(s) | 870 |
| VpaK11BI | GGWCC | 1 cut(s) | 9 |
| XapI | RAATTY | 1 cut(s) | 335 |
| XceI | RCATGY | 2 cut(s) | 244, 619 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 775 |
| XmaI | CCCGGG | 1 cut(s) | 284 |
| XmaJI | CCTAGG | 2 cut(s) | 637, 742 |
| XmnI | GAANNNNTTC | 1 cut(s) | 173 |
| XspI | CTAG | 4 cut(s) | 182, 612, 638, 743 |
| ZrmI | AGTACT | 1 cut(s) | 446 |
| Zsp2I | ATGCAT | 2 cut(s) | 359, 621 |