Rroxscaffold_2G00133200

Dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000167
Physical Location & Seq
Reverse (-)
70321703 .. 70323448
1746 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00133200.1

Sequence Viewer

Length: 1041 bp
ATGGGCTGCAAGAGATTCATCTGGTGTTCTATCTCCTTCAGCTTCTCCAGAAGGGAATCCGGAGAGAAAGACGTGACATTCAAAGTGTTGTACTGTGGGATTTGCCATTCGGACCTTCACATGGTCAAGAATGAATGGGGCTTCTCTTCCTATCCTCTGGTTCCCGGGCATGAGATTGTCGGTGAAGTGACGGAAGTAGGGAGCAATGTACAAAAATTCAAAGTTGGAGACAAAGTCGGTGTTGGATGCATGGTGGGAGCTTGCCGATCTTGTGATAGTTGTACCGACCATCTTGAGAACTACTGCCCCAAACAGATACTCACGTACGGTGCGAAGTACTACGACGGAACCACCACCTATGGCGGCTACTCTGACATAATGGTGGCAGATGAACACTTCGTAGTCCGTATCCCGGACAACCTACCCCTTGATTGTGCTGCTCCTCTCCTATGTGCCGGAATCACAACCTACAGCCCGTTGAGATATTTTGGACTTGACAAGCCCGGCATGCATGTGGGCGTGGTTGGCCTAGGTGGTTTAGGCCACGTCGCTGTGAAGTTTGCCAAGGCAATGGGAGTGAAGGTAACAGTGATCAGTACGTCCCCTAATAAGGAGGAGGAAGCAGTTAAACACCTAGGAGCTGATTCCTTTTTGGTTAGTCGTAACCAAGATCAAATGCAGGCTGCCATTGGTACCATGGATGGGATCATTGACACAGTTTCTGCACAACATCCTCTCTTGCCTTTGATTGGTTTGTTGAAGTCTCATGGAAAGCTTGTTATGGTTGGTGCACCAGAAAAGCCTCTTGAGCTTCCGGTTTTTCCTTTACTCACGGGAAGGAAGATGGTAGCTGGTAGCGGCATTGGAGGTATGAAGGAGACACAAGAGATGATCGATTTTGCAGCCAAGCACAACATAACAGCAGACATCGAGGTTATCCCAATTGACTACTTGAACACTGCCATGGAGCGCCTTGCCAAAGCAGACGTCAGATACCGTTTTGTCATTGACATTGGAAACACACTGAAGGCTAGCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

346

Amino Acids

37.49

Weight (kDa)

6.41

Isoelectric Point (pI)

30.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 21 - 139 7.3e-28 Alcohol dehydrogenase GroES-like domain
2-Hacid_dh_C PF02826 161 - 211 6.9e-06 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
AlaDh_PNT_C PF01262 171 - 241 3.9e-06 Alanine dehydrogenase/PNT, C-terminal domain
ADH_zinc_N PF00107 179 - 302 4.3e-22 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000167)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G37980 AT4G37980 AT4G37990
fragaria_vesca FvH4_1g19210 FvH4_1g19220 FvH4_1g19240 FvH4_1g19240 FvH4_1g19261 FvH4_1g19262 FvH4_1g19263 FvH4_1g19263 FvH4_1g19265 FvH4_1g19281 FvH4_1g19281 FvH4_7g07240 FvH4_7g07240 FvH4_7g07240
malus_domestica MD01G1042500.v1.1 MD01G1042600.v1.1 MD01G1042800.v1.1 MD01G1042900.v1.1 MD05G1034100.v1.1 MD10G1035900.v1.1 MD10G1036000.v1.1 MD10G1155000.v1.1 MD10G1155100.v1.1 MD10G1155200.v1.1 MD10G1155400.v1.1 MD10G1155700.v1.1 MD10G1155800.v1.1 MD15G1308800.v1.1 MD15G1308900.v1.1
prunus_persica Prupe.6G205600_v2.0.a1 Prupe.6G205700_v2.0.a1 Prupe.6G205800_v2.0.a1 Prupe.6G205900_v2.0.a1 Prupe.6G206300_v2.0.a1 Prupe.6G206800_v2.0.a1 Prupe.6G207100_v2.0.a1 Prupe.6G207200_v2.0.a1 Prupe.6G207300_v2.0.a1 Prupe.6G207400_v2.0.a1 Prupe.6G207500_v2.0.a1 Prupe.6G207600_v2.0.a1 Prupe.6G207700_v2.0.a1 Prupe.6G207900_v2.0.a1
pyrus_communis pycom01g06970 pycom01g06980 pycom01g06990 pycom05g02460 pycom08g00750 pycom10g13410 pycom15g27310 pycom15g27320
rosa_chinensis RchiOBHm_Chr1g0314861 RchiOBHm_Chr1g0345441 RchiOBHm_Chr1g0345451 RchiOBHm_Chr1g0345621 RchiOBHm_Chr1g0345751 RchiOBHm_Chr1g0345861 RchiOBHm_Chr1g0345871 RchiOBHm_Chr1g0345891 RchiOBHm_Chr2g0094851 RchiOBHm_Chr2g0110471 RchiOBHm_Chr2g0110511 RchiOBHm_Chr2g0110531 RchiOBHm_Chr2g0110541 RchiOBHm_Chr2g0110571 RchiOBHm_Chr5g0039381 RchiOBHm_Chr5g0039391 RchiOBHm_Chr6g0270311 RchiOBHm_Chr6g0270331 RchiOBHm_Chr6g0270411 RchiOBHm_Chr6g0270431
rosa_laevigata RLG00000000515 RLG00000013793 RLG00000013797 RLG00000013799 RLG00000016525 RLG00000017808 RLG00000017811 RLG00000017813 RLG00000017816 RLG00000017819 RLG00000017822 RLG00000017824 RLG00000017825 RLG00000028835
rosa_multiflora Rmu_co8225458.1_g000001 Rmu_sc0000389.1_g000006 Rmu_sc0000389.1_g000014 Rmu_sc0000389.1_g000023 Rmu_sc0001292.1_g000012 Rmu_sc0001292.1_g000026 Rmu_sc0002145.1_g000003 Rmu_sc0002329.1_g000033 Rmu_sc0002833.1_g000044 Rmu_sc0004316.1_g000012 Rmu_sc0004439.1_g000003 Rmu_sc0006422.1_g000028 Rmu_sc0009479.1_g000012 Rmu_sc0010489.1_g000009 Rmu_sc0010489.1_g000010 Rmu_sc0017837.1_g000003 Rmu_sc0032320.1_g000001
rosa_roxburghii Rroxscaffold_1G00050770 Rroxscaffold_2G00133110 Rroxscaffold_2G00133140 Rroxscaffold_2G00133200 Rroxscaffold_2G00133270 Rroxscaffold_2G00133290 Rroxscaffold_2G00133300 Rroxscaffold_2G00133350 Rroxscaffold_4G00292490 Rroxscaffold_4G00309020 Rroxscaffold_4G00331740 Rroxscaffold_4G00331800 Rroxscaffold_6G00404470 Rroxscaffold_7G00197950 Rroxscaffold_7G00197990 Rroxscaffold_7G00198020
rosa_rugosa Rorug01G0002500 Rorug02G0047200 Rorug02G0047300 Rorug02G0047300 Rorug02G0047400 Rorug02G0168000 Rorug02G0168000 Rorug02G0168100 Rorug02G0168100 Rorug02G0168400.1 Rorug03G0157600 Rorug03G0157700 Rorug05G0112600 Rorug06G0054600 Rorug06G0054700
rosa_samantha Rh1AG013300 Rh1AG195600 Rh1AG195700 Rh1AG196000 Rh1BG004900 Rh1BG114300 Rh2AG132300 Rh2BG095500 Rh2BG229700 Rh2BG229800 Rh2CG097400 Rh2CG221500 Rh2CG221800 Rh2CG222100 Rh2CG222200 Rh2CG222500 Rh2CG222600 Rh2CG345000 Rh5BG269700 Rh6AG172900 Rh6AG173100 Rh6CG080500 Rh6DG075900 Rh6DG164700 Rh6DG164900 Rh6DG165200 Rh6DG165300
rosa_wichuraiana Rw0G015390 Rw1G000600 Rw1G016130 Rw1G016190 Rw2G007140 Rw2G016970 Rw2G016990 Rw2G017000 Rw3G018910 Rw3G018920 Rw3G018930 Rw5G024830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 990
Acc65I GGTACC 1 cut(s) 692
AccB1I GGYRCC 1 cut(s) 692
AccIII TCCGGA 1 cut(s) 59
AciI CCGC 2 cut(s) 363, 858
AclWI GGATC 1 cut(s) 713
AcsI RAATTY 1 cut(s) 215
AcuI CTGAAG 1 cut(s) 22
AcyI GRCGYC 1 cut(s) 987
AfaI GTAC 7 cut(s) 92, 210, 283, 326, 338, 598, 694
AfiI CCNNNNNNNGG 5 cut(s) 121, 412, 610, 702, 749
AgsI TTSAA 4 cut(s) 82, 220, 760, 955
AjiI CACGTC 2 cut(s) 73, 547
AluBI AGCT 7 cut(s) 42, 260, 641, 775, 811, 851, 1035
AluI AGCT 7 cut(s) 42, 260, 641, 775, 811, 851, 1035
Alw21I GWGCWC 1 cut(s) 793
Alw26I GTCTC 3 cut(s) 222, 768, 872
Alw44I GTGCAC 1 cut(s) 789
AlwI GGATC 1 cut(s) 713
AlwNI CAGNNNCTG 1 cut(s) 722
Ama87I CYCGRG 1 cut(s) 164
Aor13HI TCCGGA 1 cut(s) 59
AoxI GGCC 2 cut(s) 526, 541
ApaLI GTGCAC 1 cut(s) 789
ApeKI GCWGC 4 cut(s) 6, 437, 683, 902
ApoI RAATTY 1 cut(s) 215
ArsI GACNNNNNNTTYG 2 cut(s) 972, 1004
Asp718I GGTACC 1 cut(s) 692
AspA2I CCTAGG 2 cut(s) 529, 634
AspLEI GCGC 1 cut(s) 972
AspS9I GGNCC 1 cut(s) 112
AsuC2I CCSGG 4 cut(s) 165, 166, 413, 504
AsuHPI GGTGA 1 cut(s) 194
AsuNHI GCTAGC 1 cut(s) 1031
AvaI CYCGRG 1 cut(s) 164
AvaII GGWCC 1 cut(s) 112
AvrII CCTAGG 2 cut(s) 529, 634
BaeGI GKGCMC 1 cut(s) 793
BaeI ACNNNNGTAYC 2 cut(s) 308, 341
BanI GGYRCC 1 cut(s) 692
Bbv12I GWGCWC 1 cut(s) 793
BbvI GCAGC 3 cut(s) 424, 670, 914
BccI CCATC 3 cut(s) 297, 695, 838
BciVI GTATCC 1 cut(s) 419
BclI TGATCA 1 cut(s) 591
BcnI CCSGG 4 cut(s) 165, 166, 413, 504
BcoDI GTCTC 3 cut(s) 222, 768, 872
BfaI CTAG 3 cut(s) 530, 635, 1032
BfmI CTRYAG 1 cut(s) 469
BfoI RGCGCY 1 cut(s) 973
BfuI GTATCC 1 cut(s) 419
BisI GCNGC 6 cut(s) 7, 364, 438, 684, 859, 903
BlnI CCTAGG 2 cut(s) 529, 634
BlsI GCNGC 6 cut(s) 8, 365, 439, 685, 860, 904
BmcAI AGTACT 1 cut(s) 338
Bme1390I CCNGG 4 cut(s) 165, 166, 413, 504
Bme18I GGWCC 1 cut(s) 112
BmeT110I CYCGRG 1 cut(s) 164
BmgBI CACGTC 2 cut(s) 73, 547
BmgT120I GGNCC 1 cut(s) 112
BmiI GGNNCC 3 cut(s) 162, 349, 694
BmrFI CCNGG 4 cut(s) 165, 166, 413, 504
BmsI GCATC 1 cut(s) 236
BmtI GCTAGC 1 cut(s) 1035
BpmI CTGGAG 1 cut(s) 31
BpuEI CTTGAG 2 cut(s) 314, 827
BpuMI CCSGG 4 cut(s) 165, 166, 413, 504
Bsa29I ATCGAT 1 cut(s) 894
BsaAI YACGTR 1 cut(s) 324
BsaHI GRCGYC 1 cut(s) 987
BsaJI CCNNGG 6 cut(s) 164, 529, 564, 634, 696, 963
BsaWI WCCGGW 2 cut(s) 59, 814
BsaXI ACNNNNNCTCC 4 cut(s) 219, 249, 567, 597
Bsc4I CCNNNNNNNGG 5 cut(s) 121, 412, 610, 702, 749
Bse3DI GCAATG 2 cut(s) 211, 576
BseAI TCCGGA 1 cut(s) 59
BseCI ATCGAT 1 cut(s) 894
BseDI CCNNGG 6 cut(s) 164, 529, 564, 634, 696, 963
BseGI GGATG 3 cut(s) 251, 706, 730
BseLI CCNNNNNNNGG 5 cut(s) 121, 412, 610, 702, 749
BseMI GCAATG 2 cut(s) 211, 576
BseRI GAGGAG 2 cut(s) 432, 629
BseSI GKGCMC 1 cut(s) 793
BseXI GCAGC 3 cut(s) 424, 670, 914
BsgI GTGCAG 1 cut(s) 708
BshFI GGCC 2 cut(s) 528, 543
BshNI GGYRCC 1 cut(s) 692
BshVI ATCGAT 1 cut(s) 894
BsiHKAI GWGCWC 1 cut(s) 793
BsiHKCI CYCGRG 1 cut(s) 164
BsiSI CCGG 6 cut(s) 60, 165, 413, 456, 504, 815
BsiWI CGTACG 1 cut(s) 324
BslFI GGGAC 1 cut(s) 586
BslI CCNNNNNNNGG 5 cut(s) 121, 412, 610, 702, 749
BsmAI GTCTC 3 cut(s) 222, 768, 872
BsmFI GGGAC 1 cut(s) 586
BsnI GGCC 2 cut(s) 528, 543
BsoBI CYCGRG 1 cut(s) 164
Bsp1286I GDGCHC 1 cut(s) 793
Bsp13I TCCGGA 1 cut(s) 59
Bsp1407I TGTACA 1 cut(s) 208
Bsp143I GATC 5 cut(s) 266, 591, 670, 705, 891
Bsp19I CCATGG 2 cut(s) 696, 963
BspACI CCGC 2 cut(s) 363, 858
BspANI GGCC 2 cut(s) 528, 543
BspDI ATCGAT 1 cut(s) 894
BspEI TCCGGA 1 cut(s) 59
BspLI GGNNCC 3 cut(s) 162, 349, 694
BspOI GCTAGC 1 cut(s) 1035
BspPI GGATC 1 cut(s) 713
BspT107I GGYRCC 1 cut(s) 692
BsrDI GCAATG 2 cut(s) 211, 576
BsrGI TGTACA 1 cut(s) 208
BssECI CCNNGG 6 cut(s) 164, 529, 564, 634, 696, 963
BssMI GATC 5 cut(s) 266, 591, 670, 705, 891
BssNI GRCGYC 1 cut(s) 987
BssT1I CCWWGG 5 cut(s) 529, 564, 634, 696, 963
Bst4CI ACNGT 5 cut(s) 95, 329, 589, 718, 998
Bst6I CTCTTC 1 cut(s) 151
BstACI GRCGYC 1 cut(s) 987
BstAUI TGTACA 1 cut(s) 208
BstBAI YACGTR 1 cut(s) 324
BstC8I GCNNGC 4 cut(s) 262, 509, 681, 1033
BstDSI CCRYGG 2 cut(s) 696, 963
BstF5I GGATG 3 cut(s) 251, 706, 730
BstH2I RGCGCY 1 cut(s) 973
BstHHI GCGC 1 cut(s) 972
BstKTI GATC 5 cut(s) 269, 594, 673, 708, 894
BstMAI GTCTC 3 cut(s) 222, 768, 872
BstMBI GATC 5 cut(s) 266, 591, 670, 705, 891
BstMWI GCNNNNNNNGC 3 cut(s) 508, 525, 808
BstNSI RCATGY 2 cut(s) 511, 515
BstSCI CCNGG 4 cut(s) 163, 164, 411, 502
BstSFI CTRYAG 1 cut(s) 469
BstSLI GKGCMC 1 cut(s) 793
BstV1I GCAGC 3 cut(s) 424, 670, 914
BstXI CCANNNNNNTGG 1 cut(s) 571
Bsu15I ATCGAT 1 cut(s) 894
BsuI GTATCC 1 cut(s) 419
BsuRI GGCC 2 cut(s) 528, 543
BsuTUI ATCGAT 1 cut(s) 894
BtgI CCRYGG 2 cut(s) 696, 963
BtrI CACGTC 2 cut(s) 73, 547
BtsCI GGATG 3 cut(s) 251, 706, 730
BtsI GCAGTG 1 cut(s) 957
BtsIMutI CAGTG 3 cut(s) 594, 957, 1022
Cac8I GCNNGC 4 cut(s) 262, 509, 681, 1033
CaiI CAGNNNCTG 1 cut(s) 722
CfoI GCGC 1 cut(s) 972
Cfr13I GGNCC 1 cut(s) 112
Cfr9I CCCGGG 1 cut(s) 164
ClaI ATCGAT 1 cut(s) 894
Csp6I GTAC 7 cut(s) 91, 209, 282, 325, 337, 597, 693
CviAII CATG 8 cut(s) 121, 170, 250, 508, 512, 697, 767, 964
CviQI GTAC 7 cut(s) 91, 209, 282, 325, 337, 597, 693
DpnI GATC 5 cut(s) 268, 593, 672, 707, 893
DpnII GATC 5 cut(s) 266, 591, 670, 705, 891
Eam1104I CTCTTC 1 cut(s) 151
EarI CTCTTC 1 cut(s) 151
Eco130I CCWWGG 5 cut(s) 529, 564, 634, 696, 963
Eco47I GGWCC 1 cut(s) 112
Eco57I CTGAAG 1 cut(s) 22
Eco88I CYCGRG 1 cut(s) 164
EcoT14I CCWWGG 5 cut(s) 529, 564, 634, 696, 963
EcoT22I ATGCAT 2 cut(s) 251, 513
ErhI CCWWGG 5 cut(s) 529, 564, 634, 696, 963
FaeI CATG 8 cut(s) 124, 173, 253, 511, 515, 700, 770, 967
FaqI GGGAC 1 cut(s) 586
FatI CATG 8 cut(s) 120, 169, 249, 507, 511, 696, 766, 963
FbaI TGATCA 1 cut(s) 591
Fnu4HI GCNGC 6 cut(s) 7, 364, 438, 684, 859, 903
FokI GGATG 3 cut(s) 258, 713, 717
Fsp4HI GCNGC 6 cut(s) 7, 364, 438, 684, 859, 903
FspBI CTAG 3 cut(s) 530, 635, 1032
GlaI GCGC 1 cut(s) 971
GluI GCNGC 6 cut(s) 7, 364, 438, 684, 859, 903
GsuI CTGGAG 1 cut(s) 31
HaeII RGCGCY 1 cut(s) 973
HaeIII GGCC 2 cut(s) 528, 543
HapII CCGG 6 cut(s) 60, 165, 413, 456, 504, 815
HhaI GCGC 1 cut(s) 972
Hin1I GRCGYC 1 cut(s) 987
Hin1II CATG 8 cut(s) 124, 173, 253, 511, 515, 700, 770, 967
Hin6I GCGC 1 cut(s) 970
HinP1I GCGC 1 cut(s) 970
HindIII AAGCTT 1 cut(s) 773
HinfI GANTC 4 cut(s) 15, 56, 459, 644
HpaII CCGG 6 cut(s) 60, 165, 413, 456, 504, 815
HphI GGTGA 1 cut(s) 194
Hpy166II GTNNAC 1 cut(s) 791
Hpy188I TCNGA 3 cut(s) 112, 373, 992
Hpy188III TCNNGA 5 cut(s) 48, 60, 127, 293, 806
Hpy8I GTNNAC 1 cut(s) 791
Hpy99I CGWCG 2 cut(s) 347, 551
HpyAV CCTTC 7 cut(s) 45, 46, 125, 574, 831, 868, 1021
HpyCH4III ACNGT 5 cut(s) 95, 329, 589, 718, 998
HpyCH4IV ACGT 5 cut(s) 72, 323, 546, 599, 987
HpyCH4V TGCA 7 cut(s) 9, 249, 511, 679, 725, 791, 902
HpyF10VI GCNNNNNNNGC 3 cut(s) 508, 525, 808
HpySE526I ACGT 5 cut(s) 72, 323, 546, 599, 987
Hsp92I GRCGYC 1 cut(s) 987
Hsp92II CATG 8 cut(s) 124, 173, 253, 511, 515, 700, 770, 967
HspAI GCGC 1 cut(s) 970
Kpn2I TCCGGA 1 cut(s) 59
KpnI GGTACC 1 cut(s) 696
Ksp22I TGATCA 1 cut(s) 591
Kzo9I GATC 5 cut(s) 266, 591, 670, 705, 891
LmnI GCTCC 5 cut(s) 201, 257, 445, 638, 967
Lsp1109I GCAGC 3 cut(s) 424, 670, 914
LweI GCATC 1 cut(s) 236
MaeI CTAG 3 cut(s) 530, 635, 1032
MaeII ACGT 5 cut(s) 72, 323, 546, 599, 987
MaeIII GTNAC 4 cut(s) 73, 187, 583, 662
MalI GATC 5 cut(s) 268, 593, 672, 707, 893
MboI GATC 5 cut(s) 266, 591, 670, 705, 891
MboII GAAGA 2 cut(s) 138, 853
MfeI CAATTG 1 cut(s) 942
MhlI GDGCHC 1 cut(s) 793
MluCI AATT 2 cut(s) 215, 942
MmeI TCCRAC 2 cut(s) 205, 223
MnlI CCTC 8 cut(s) 165, 453, 607, 610, 744, 813, 860, 925
Mph1103I ATGCAT 2 cut(s) 251, 513
MroI TCCGGA 1 cut(s) 59
MseI TTAA 2 cut(s) 627, 1039
MslI CAYNNNNRTG 3 cut(s) 380, 512, 962
MspI CCGG 6 cut(s) 60, 165, 413, 456, 504, 815
MspR9I CCNGG 4 cut(s) 165, 166, 413, 504
MunI CAATTG 1 cut(s) 942
MwoI GCNNNNNNNGC 3 cut(s) 508, 525, 808
NciI CCSGG 4 cut(s) 165, 166, 413, 504
NcoI CCATGG 2 cut(s) 696, 963
NdeII GATC 5 cut(s) 266, 591, 670, 705, 891
NheI GCTAGC 1 cut(s) 1031
NlaIII CATG 8 cut(s) 124, 173, 253, 511, 515, 700, 770, 967
NlaIV GGNNCC 3 cut(s) 162, 349, 694
NmuCI GTSAC 2 cut(s) 73, 187
NsiI ATGCAT 2 cut(s) 251, 513
NspI RCATGY 2 cut(s) 511, 515
PaeI GCATGC 1 cut(s) 511
PcsI WCGNNNNNNNCGW 1 cut(s) 329
PfeI GAWTC 4 cut(s) 15, 56, 459, 644
Pfl23II CGTACG 1 cut(s) 324
PflFI GACNNNGTC 1 cut(s) 233
PfoI TCCNGGA 1 cut(s) 411
PkrI GCNGC 6 cut(s) 8, 365, 439, 685, 860, 904
Ppu21I YACGTR 1 cut(s) 324
PspLI CGTACG 1 cut(s) 324
PspN4I GGNNCC 3 cut(s) 162, 349, 694
PspPI GGNCC 1 cut(s) 112
PstNI CAGNNNCTG 1 cut(s) 722
PsyI GACNNNGTC 1 cut(s) 233
RsaI GTAC 7 cut(s) 92, 210, 283, 326, 338, 598, 694
RsaNI GTAC 7 cut(s) 91, 209, 282, 325, 337, 597, 693
RseI CAYNNNNRTG 3 cut(s) 380, 512, 962
SaqAI TTAA 2 cut(s) 627, 1039
SatI GCNGC 6 cut(s) 7, 364, 438, 684, 859, 903
Sau3AI GATC 5 cut(s) 266, 591, 670, 705, 891
Sau96I GGNCC 1 cut(s) 112
ScaI AGTACT 1 cut(s) 338
ScrFI CCNGG 4 cut(s) 165, 166, 413, 504
SduI GDGCHC 1 cut(s) 793
SfaNI GCATC 1 cut(s) 236
SfcI CTRYAG 1 cut(s) 469
SinI GGWCC 1 cut(s) 112
SmaI CCCGGG 1 cut(s) 166
SmiMI CAYNNNNRTG 3 cut(s) 380, 512, 962
SmlI CTYRAG 2 cut(s) 293, 806
SmoI CTYRAG 2 cut(s) 293, 806
SphI GCATGC 1 cut(s) 511
Sse9I AATT 2 cut(s) 215, 942
SsiI CCGC 2 cut(s) 363, 858
SspMI CTAG 3 cut(s) 530, 635, 1032
StyD4I CCNGG 4 cut(s) 163, 164, 411, 502
StyI CCWWGG 5 cut(s) 529, 564, 634, 696, 963
TaaI ACNGT 5 cut(s) 95, 329, 589, 718, 998
TaiI ACGT 5 cut(s) 75, 326, 549, 602, 990
TaqI TCGA 2 cut(s) 894, 930
TasI AATT 2 cut(s) 215, 942
TatI WGTACW 3 cut(s) 90, 208, 336
TauI GCSGC 2 cut(s) 366, 861
TfiI GAWTC 4 cut(s) 15, 56, 459, 644
Tru1I TTAA 2 cut(s) 627, 1039
Tru9I TTAA 2 cut(s) 627, 1039
TscAI CASTG 3 cut(s) 594, 964, 1029
TseFI GTSAC 2 cut(s) 73, 187
TseI GCWGC 4 cut(s) 6, 437, 683, 902
Tsp45I GTSAC 2 cut(s) 73, 187
TspDTI ATGAA 4 cut(s) 7, 147, 405, 887
TspGWI ACGGA 3 cut(s) 206, 360, 395
TspMI CCCGGG 1 cut(s) 164
TspRI CASTG 3 cut(s) 594, 964, 1029
Tth111I GACNNNGTC 1 cut(s) 233
VneI GTGCAC 1 cut(s) 789
VpaK11BI GGWCC 1 cut(s) 112
XapI RAATTY 1 cut(s) 215
XceI RCATGY 2 cut(s) 511, 515
XcmI CCANNNNNNNNNTGG 1 cut(s) 694
XmaI CCCGGG 1 cut(s) 164
XmaJI CCTAGG 2 cut(s) 529, 634
XspI CTAG 3 cut(s) 530, 635, 1032
ZraI GACGTC 1 cut(s) 988
ZrmI AGTACT 1 cut(s) 338
Zsp2I ATGCAT 2 cut(s) 251, 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.