Rh2CG222500

Dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000167
Physical Location & Seq
Forward (+)
22386628 .. 22387796
1169 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG222500.1

Sequence Viewer

Length: 846 bp
ATGTCTAACGAGCAAGAACGCCCCAGGATGGCATTTGGATGGGCTGCAAGAGATTCATCTGGTGTTCTCTCTCCCTTCAGTTTCTCCAGAAGGGAATCCGGAGACAAAGACGTGACATTCAAAGTGTTGTACTGTGGGATTTGCCATTCGGACCTTCACATGGTCAAGAATGAATGGGGCTTCTCTACCTATCCTCTGGTTCCCGGGCATGAGATTGTCGGTGAAGTAACGGAAGTAGGGAGCAATGTACAAAAATTCAAAGTTGGAGACAAAGTCGATGTTGGAGGCATAGTGGGAGCTTGTCGATCTTGTGATAGCTGTACCGACCATATTGAGAACTACTGCCCCAAACTAATACTCACGTACAGTGCCAAGTACTATGACGGAACCACCACATATGGCGGTTACTCTGACATTATGGTTGCAGATGAACACTTCGTAGTCCGTATCCCGGACAACCTACCCCTTGATTGTGCTGCTCCTCTCCTATGTGCCGGAATCACAACCTACAGCCCGTTGAGATATTTTGGACTTGACAAGCCTGGCATGCATGTGGGCGTGGTTGGCCTAGGTGGTTTAGGCCACGTCGCAGTGAAGTTTGCCAAGGCAATGGGAGTGAAGGTTACAGTGATCAGTACGTCCCCTAATAAGAAGGAGGAAGCAGTTAAACACCTAGGAGCTGATTCGTTTTTGGTTAGTCGTGACCAAGATCAAATGCAGGCTGCCATTGGTACCATGGATGGGATCATTGACACATTCTGCCCAACATCCTCTCTTGCCTTTGATTGGTTTGTTGAAGTCTCATGGAAAGCTTGTTATGCTTGGTGTACCAGAAAAGCCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

281

Amino Acids

30.66

Weight (kDa)

5.9

Isoelectric Point (pI)

41.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 34 - 152 1.1e-26 Alcohol dehydrogenase GroES-like domain
2-Hacid_dh_C PF02826 174 - 224 6.6e-06 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
ADH_zinc_N PF00107 192 - 254 1.6e-09 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000167)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G37980 AT4G37980 AT4G37990
fragaria_vesca FvH4_1g19210 FvH4_1g19220 FvH4_1g19240 FvH4_1g19240 FvH4_1g19261 FvH4_1g19262 FvH4_1g19263 FvH4_1g19263 FvH4_1g19265 FvH4_1g19281 FvH4_1g19281 FvH4_7g07240 FvH4_7g07240 FvH4_7g07240
malus_domestica MD01G1042500.v1.1 MD01G1042600.v1.1 MD01G1042800.v1.1 MD01G1042900.v1.1 MD05G1034100.v1.1 MD10G1035900.v1.1 MD10G1036000.v1.1 MD10G1155000.v1.1 MD10G1155100.v1.1 MD10G1155200.v1.1 MD10G1155400.v1.1 MD10G1155700.v1.1 MD10G1155800.v1.1 MD15G1308800.v1.1 MD15G1308900.v1.1
prunus_persica Prupe.6G205600_v2.0.a1 Prupe.6G205700_v2.0.a1 Prupe.6G205800_v2.0.a1 Prupe.6G205900_v2.0.a1 Prupe.6G206300_v2.0.a1 Prupe.6G206800_v2.0.a1 Prupe.6G207100_v2.0.a1 Prupe.6G207200_v2.0.a1 Prupe.6G207300_v2.0.a1 Prupe.6G207400_v2.0.a1 Prupe.6G207500_v2.0.a1 Prupe.6G207600_v2.0.a1 Prupe.6G207700_v2.0.a1 Prupe.6G207900_v2.0.a1
pyrus_communis pycom01g06970 pycom01g06980 pycom01g06990 pycom05g02460 pycom08g00750 pycom10g13410 pycom15g27310 pycom15g27320
rosa_chinensis RchiOBHm_Chr1g0314861 RchiOBHm_Chr1g0345441 RchiOBHm_Chr1g0345451 RchiOBHm_Chr1g0345621 RchiOBHm_Chr1g0345751 RchiOBHm_Chr1g0345861 RchiOBHm_Chr1g0345871 RchiOBHm_Chr1g0345891 RchiOBHm_Chr2g0094851 RchiOBHm_Chr2g0110471 RchiOBHm_Chr2g0110511 RchiOBHm_Chr2g0110531 RchiOBHm_Chr2g0110541 RchiOBHm_Chr2g0110571 RchiOBHm_Chr5g0039381 RchiOBHm_Chr5g0039391 RchiOBHm_Chr6g0270311 RchiOBHm_Chr6g0270331 RchiOBHm_Chr6g0270411 RchiOBHm_Chr6g0270431
rosa_laevigata RLG00000000515 RLG00000013793 RLG00000013797 RLG00000013799 RLG00000016525 RLG00000017808 RLG00000017811 RLG00000017813 RLG00000017816 RLG00000017819 RLG00000017822 RLG00000017824 RLG00000017825 RLG00000028835
rosa_multiflora Rmu_co8225458.1_g000001 Rmu_sc0000389.1_g000006 Rmu_sc0000389.1_g000014 Rmu_sc0000389.1_g000023 Rmu_sc0001292.1_g000012 Rmu_sc0001292.1_g000026 Rmu_sc0002145.1_g000003 Rmu_sc0002329.1_g000033 Rmu_sc0002833.1_g000044 Rmu_sc0004316.1_g000012 Rmu_sc0004439.1_g000003 Rmu_sc0006422.1_g000028 Rmu_sc0009479.1_g000012 Rmu_sc0010489.1_g000009 Rmu_sc0010489.1_g000010 Rmu_sc0017837.1_g000003 Rmu_sc0032320.1_g000001
rosa_roxburghii Rroxscaffold_1G00050770 Rroxscaffold_2G00133110 Rroxscaffold_2G00133140 Rroxscaffold_2G00133200 Rroxscaffold_2G00133270 Rroxscaffold_2G00133290 Rroxscaffold_2G00133300 Rroxscaffold_2G00133350 Rroxscaffold_4G00292490 Rroxscaffold_4G00309020 Rroxscaffold_4G00331740 Rroxscaffold_4G00331800 Rroxscaffold_6G00404470 Rroxscaffold_7G00197950 Rroxscaffold_7G00197990 Rroxscaffold_7G00198020
rosa_rugosa Rorug01G0002500 Rorug02G0047200 Rorug02G0047300 Rorug02G0047300 Rorug02G0047400 Rorug02G0168000 Rorug02G0168000 Rorug02G0168100 Rorug02G0168100 Rorug02G0168400.1 Rorug03G0157600 Rorug03G0157700 Rorug05G0112600 Rorug06G0054600 Rorug06G0054700
rosa_samantha Rh1AG013300 Rh1AG195600 Rh1AG195700 Rh1AG196000 Rh1BG004900 Rh1BG114300 Rh2AG132300 Rh2BG095500 Rh2BG229700 Rh2BG229800 Rh2CG097400 Rh2CG221500 Rh2CG221800 Rh2CG222100 Rh2CG222200 Rh2CG222500 Rh2CG222600 Rh2CG345000 Rh5BG269700 Rh6AG172900 Rh6AG173100 Rh6CG080500 Rh6DG075900 Rh6DG164700 Rh6DG164900 Rh6DG165200 Rh6DG165300
rosa_wichuraiana Rw0G015390 Rw1G000600 Rw1G016130 Rw1G016190 Rw2G007140 Rw2G016970 Rw2G016990 Rw2G017000 Rw3G018910 Rw3G018920 Rw3G018930 Rw5G024830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 731
AccB1I GGYRCC 1 cut(s) 731
AccIII TCCGGA 1 cut(s) 98
AciI CCGC 1 cut(s) 402
AclWI GGATC 1 cut(s) 752
AcsI RAATTY 1 cut(s) 254
AcuI CTGAAG 1 cut(s) 61
AfaI GTAC 8 cut(s) 131, 249, 322, 365, 377, 637, 733, 829
AfiI CCNNNNNNNGG 5 cut(s) 28, 160, 451, 741, 786
AgsI TTSAA 3 cut(s) 121, 259, 797
AjiI CACGTC 2 cut(s) 112, 586
AjnI CCWGG 2 cut(s) 23, 541
AluBI AGCT 4 cut(s) 299, 318, 680, 812
AluI AGCT 4 cut(s) 299, 318, 680, 812
Alw26I GTCTC 3 cut(s) 96, 261, 805
AlwI GGATC 1 cut(s) 752
Ama87I CYCGRG 1 cut(s) 203
Aor13HI TCCGGA 1 cut(s) 98
AoxI GGCC 2 cut(s) 565, 580
ApeKI GCWGC 3 cut(s) 44, 476, 722
ApoI RAATTY 1 cut(s) 254
Asp718I GGTACC 1 cut(s) 731
AspA2I CCTAGG 2 cut(s) 568, 673
AspS9I GGNCC 1 cut(s) 151
AsuC2I CCSGG 3 cut(s) 204, 205, 452
AsuHPI GGTGA 1 cut(s) 233
AvaI CYCGRG 1 cut(s) 203
AvaII GGWCC 1 cut(s) 151
AvrII CCTAGG 2 cut(s) 568, 673
BanI GGYRCC 1 cut(s) 731
BbvI GCAGC 3 cut(s) 31, 463, 709
BccI CCATC 3 cut(s) 22, 33, 734
BciT130I CCWGG 2 cut(s) 25, 543
BciVI GTATCC 1 cut(s) 458
BclI TGATCA 1 cut(s) 630
BcnI CCSGG 3 cut(s) 204, 205, 452
BcoDI GTCTC 3 cut(s) 96, 261, 805
BfaI CTAG 2 cut(s) 569, 674
BfmI CTRYAG 1 cut(s) 508
BfuI GTATCC 1 cut(s) 458
BisI GCNGC 3 cut(s) 45, 477, 723
BlnI CCTAGG 2 cut(s) 568, 673
BlsI GCNGC 3 cut(s) 46, 478, 724
BmcAI AGTACT 1 cut(s) 377
Bme1390I CCNGG 5 cut(s) 25, 204, 205, 452, 543
Bme18I GGWCC 1 cut(s) 151
BmeT110I CYCGRG 1 cut(s) 203
BmgBI CACGTC 2 cut(s) 112, 586
BmgT120I GGNCC 1 cut(s) 151
BmiI GGNNCC 3 cut(s) 201, 388, 733
BmrFI CCNGG 5 cut(s) 25, 204, 205, 452, 543
BpmI CTGGAG 1 cut(s) 70
BpuMI CCSGG 3 cut(s) 204, 205, 452
BsaAI YACGTR 1 cut(s) 363
BsaJI CCNNGG 6 cut(s) 23, 203, 568, 603, 673, 735
BsaWI WCCGGW 1 cut(s) 98
BsaXI ACNNNNNCTCC 6 cut(s) 258, 276, 288, 306, 606, 636
Bsc4I CCNNNNNNNGG 5 cut(s) 28, 160, 451, 741, 786
Bse3DI GCAATG 2 cut(s) 250, 615
BseAI TCCGGA 1 cut(s) 98
BseBI CCWGG 2 cut(s) 25, 543
BseDI CCNNGG 6 cut(s) 23, 203, 568, 603, 673, 735
BseGI GGATG 4 cut(s) 33, 44, 745, 767
BseLI CCNNNNNNNGG 5 cut(s) 28, 160, 451, 741, 786
BseMI GCAATG 2 cut(s) 250, 615
BseRI GAGGAG 1 cut(s) 471
BseXI GCAGC 3 cut(s) 31, 463, 709
BshFI GGCC 2 cut(s) 567, 582
BshNI GGYRCC 1 cut(s) 731
BsiHKCI CYCGRG 1 cut(s) 203
BsiSI CCGG 4 cut(s) 99, 204, 452, 495
BslFI GGGAC 1 cut(s) 625
BslI CCNNNNNNNGG 5 cut(s) 28, 160, 451, 741, 786
BsmAI GTCTC 3 cut(s) 96, 261, 805
BsmFI GGGAC 1 cut(s) 625
BsnI GGCC 2 cut(s) 567, 582
BsoBI CYCGRG 1 cut(s) 203
Bsp13I TCCGGA 1 cut(s) 98
Bsp1407I TGTACA 1 cut(s) 247
Bsp143I GATC 4 cut(s) 305, 630, 709, 744
Bsp19I CCATGG 1 cut(s) 735
BspACI CCGC 1 cut(s) 402
BspANI GGCC 2 cut(s) 567, 582
BspEI TCCGGA 1 cut(s) 98
BspLI GGNNCC 3 cut(s) 201, 388, 733
BspPI GGATC 1 cut(s) 752
BspT107I GGYRCC 1 cut(s) 731
BsrDI GCAATG 2 cut(s) 250, 615
BsrGI TGTACA 1 cut(s) 247
BssECI CCNNGG 6 cut(s) 23, 203, 568, 603, 673, 735
BssMI GATC 4 cut(s) 305, 630, 709, 744
BssT1I CCWWGG 4 cut(s) 568, 603, 673, 735
Bst2UI CCWGG 2 cut(s) 25, 543
Bst4CI ACNGT 3 cut(s) 134, 368, 628
BstAUI TGTACA 1 cut(s) 247
BstBAI YACGTR 1 cut(s) 363
BstC8I GCNNGC 2 cut(s) 548, 720
BstDSI CCRYGG 1 cut(s) 735
BstF5I GGATG 4 cut(s) 33, 44, 745, 767
BstKTI GATC 4 cut(s) 308, 633, 712, 747
BstMAI GTCTC 3 cut(s) 96, 261, 805
BstMBI GATC 4 cut(s) 305, 630, 709, 744
BstMWI GCNNNNNNNGC 3 cut(s) 547, 564, 818
BstNI CCWGG 2 cut(s) 25, 543
BstNSI RCATGY 2 cut(s) 550, 554
BstSCI CCNGG 5 cut(s) 23, 202, 203, 450, 541
BstSFI CTRYAG 1 cut(s) 508
BstV1I GCAGC 3 cut(s) 31, 463, 709
BstXI CCANNNNNNTGG 1 cut(s) 610
BsuI GTATCC 1 cut(s) 458
BsuRI GGCC 2 cut(s) 567, 582
BtgI CCRYGG 1 cut(s) 735
BtrI CACGTC 2 cut(s) 112, 586
BtsCI GGATG 4 cut(s) 33, 44, 745, 767
BtsI GCAGTG 1 cut(s) 597
BtsIMutI CAGTG 3 cut(s) 373, 597, 633
Cac8I GCNNGC 2 cut(s) 548, 720
Cfr13I GGNCC 1 cut(s) 151
Cfr9I CCCGGG 1 cut(s) 203
Csp6I GTAC 8 cut(s) 130, 248, 321, 364, 376, 636, 732, 828
CviAII CATG 6 cut(s) 160, 209, 547, 551, 736, 804
CviQI GTAC 8 cut(s) 130, 248, 321, 364, 376, 636, 732, 828
DpnI GATC 4 cut(s) 307, 632, 711, 746
DpnII GATC 4 cut(s) 305, 630, 709, 744
Eco130I CCWWGG 4 cut(s) 568, 603, 673, 735
Eco47I GGWCC 1 cut(s) 151
Eco57I CTGAAG 1 cut(s) 61
Eco88I CYCGRG 1 cut(s) 203
EcoRII CCWGG 2 cut(s) 23, 541
EcoT14I CCWWGG 4 cut(s) 568, 603, 673, 735
EcoT22I ATGCAT 1 cut(s) 552
ErhI CCWWGG 4 cut(s) 568, 603, 673, 735
FaeI CATG 6 cut(s) 163, 212, 550, 554, 739, 807
FaqI GGGAC 1 cut(s) 625
FatI CATG 6 cut(s) 159, 208, 546, 550, 735, 803
FauNDI CATATG 1 cut(s) 397
FbaI TGATCA 1 cut(s) 630
Fnu4HI GCNGC 3 cut(s) 45, 477, 723
FokI GGATG 4 cut(s) 40, 51, 752, 754
Fsp4HI GCNGC 3 cut(s) 45, 477, 723
FspBI CTAG 2 cut(s) 569, 674
GluI GCNGC 3 cut(s) 45, 477, 723
GsuI CTGGAG 1 cut(s) 70
HaeIII GGCC 2 cut(s) 567, 582
HapII CCGG 4 cut(s) 99, 204, 452, 495
Hin1II CATG 6 cut(s) 163, 212, 550, 554, 739, 807
HindIII AAGCTT 1 cut(s) 810
HinfI GANTC 4 cut(s) 53, 95, 498, 683
HpaII CCGG 4 cut(s) 99, 204, 452, 495
HphI GGTGA 1 cut(s) 233
Hpy166II GTNNAC 1 cut(s) 828
Hpy188I TCNGA 2 cut(s) 151, 412
Hpy188III TCNNGA 5 cut(s) 87, 99, 166, 701, 843
Hpy8I GTNNAC 1 cut(s) 828
Hpy99I CGWCG 1 cut(s) 590
HpyAV CCTTC 5 cut(s) 84, 85, 164, 613, 646
HpyCH4III ACNGT 3 cut(s) 134, 368, 628
HpyCH4IV ACGT 4 cut(s) 111, 362, 585, 638
HpyCH4V TGCA 4 cut(s) 47, 425, 550, 718
HpyF10VI GCNNNNNNNGC 3 cut(s) 547, 564, 818
HpySE526I ACGT 4 cut(s) 111, 362, 585, 638
Hsp92II CATG 6 cut(s) 163, 212, 550, 554, 739, 807
Kpn2I TCCGGA 1 cut(s) 98
KpnI GGTACC 1 cut(s) 735
Ksp22I TGATCA 1 cut(s) 630
Kzo9I GATC 4 cut(s) 305, 630, 709, 744
LmnI GCTCC 4 cut(s) 240, 296, 484, 677
Lsp1109I GCAGC 3 cut(s) 31, 463, 709
MaeI CTAG 2 cut(s) 569, 674
MaeII ACGT 4 cut(s) 111, 362, 585, 638
MaeIII GTNAC 5 cut(s) 112, 226, 404, 622, 701
MalI GATC 4 cut(s) 307, 632, 711, 746
MboI GATC 4 cut(s) 305, 630, 709, 744
MluCI AATT 1 cut(s) 254
MmeI TCCRAC 2 cut(s) 244, 262
MnlI CCTC 5 cut(s) 204, 278, 492, 649, 781
Mph1103I ATGCAT 1 cut(s) 552
MroI TCCGGA 1 cut(s) 98
MseI TTAA 1 cut(s) 666
MslI CAYNNNNRTG 2 cut(s) 37, 551
MspI CCGG 4 cut(s) 99, 204, 452, 495
MspR9I CCNGG 5 cut(s) 25, 204, 205, 452, 543
MvaI CCWGG 2 cut(s) 25, 543
MwoI GCNNNNNNNGC 3 cut(s) 547, 564, 818
NciI CCSGG 3 cut(s) 204, 205, 452
NcoI CCATGG 1 cut(s) 735
NdeI CATATG 1 cut(s) 397
NdeII GATC 4 cut(s) 305, 630, 709, 744
NlaIII CATG 6 cut(s) 163, 212, 550, 554, 739, 807
NlaIV GGNNCC 3 cut(s) 201, 388, 733
NmuCI GTSAC 2 cut(s) 112, 701
NsiI ATGCAT 1 cut(s) 552
NspI RCATGY 2 cut(s) 550, 554
PaeI GCATGC 1 cut(s) 550
PfeI GAWTC 4 cut(s) 53, 95, 498, 683
PflFI GACNNNGTC 1 cut(s) 272
PfoI TCCNGGA 1 cut(s) 450
PkrI GCNGC 3 cut(s) 46, 478, 724
Ppu21I YACGTR 1 cut(s) 363
Psp6I CCWGG 2 cut(s) 23, 541
PspGI CCWGG 2 cut(s) 23, 541
PspN4I GGNNCC 3 cut(s) 201, 388, 733
PspPI GGNCC 1 cut(s) 151
PsyI GACNNNGTC 1 cut(s) 272
RsaI GTAC 8 cut(s) 131, 249, 322, 365, 377, 637, 733, 829
RsaNI GTAC 8 cut(s) 130, 248, 321, 364, 376, 636, 732, 828
RseI CAYNNNNRTG 2 cut(s) 37, 551
SaqAI TTAA 1 cut(s) 666
SatI GCNGC 3 cut(s) 45, 477, 723
Sau3AI GATC 4 cut(s) 305, 630, 709, 744
Sau96I GGNCC 1 cut(s) 151
ScaI AGTACT 1 cut(s) 377
ScrFI CCNGG 5 cut(s) 25, 204, 205, 452, 543
SfcI CTRYAG 1 cut(s) 508
SinI GGWCC 1 cut(s) 151
SmaI CCCGGG 1 cut(s) 205
SmiMI CAYNNNNRTG 2 cut(s) 37, 551
SphI GCATGC 1 cut(s) 550
Sse9I AATT 1 cut(s) 254
SsiI CCGC 1 cut(s) 402
SspMI CTAG 2 cut(s) 569, 674
StyD4I CCNGG 5 cut(s) 23, 202, 203, 450, 541
StyI CCWWGG 4 cut(s) 568, 603, 673, 735
TaaI ACNGT 3 cut(s) 134, 368, 628
TaiI ACGT 4 cut(s) 114, 365, 588, 641
TaqI TCGA 2 cut(s) 276, 304
TasI AATT 1 cut(s) 254
TatI WGTACW 3 cut(s) 129, 247, 375
TfiI GAWTC 4 cut(s) 53, 95, 498, 683
Tru1I TTAA 1 cut(s) 666
Tru9I TTAA 1 cut(s) 666
TscAI CASTG 3 cut(s) 373, 597, 633
TseFI GTSAC 2 cut(s) 112, 701
TseI GCWGC 3 cut(s) 44, 476, 722
Tsp45I GTSAC 2 cut(s) 112, 701
TspDTI ATGAA 3 cut(s) 45, 186, 444
TspGWI ACGGA 3 cut(s) 245, 399, 434
TspMI CCCGGG 1 cut(s) 203
TspRI CASTG 3 cut(s) 373, 597, 633
Tth111I GACNNNGTC 1 cut(s) 272
VpaK11BI GGWCC 1 cut(s) 151
XapI RAATTY 1 cut(s) 254
XceI RCATGY 2 cut(s) 550, 554
XcmI CCANNNNNNNNNTGG 1 cut(s) 733
XmaI CCCGGG 1 cut(s) 203
XmaJI CCTAGG 2 cut(s) 568, 673
XspI CTAG 2 cut(s) 569, 674
ZrmI AGTACT 1 cut(s) 377
Zsp2I ATGCAT 1 cut(s) 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.