MD10G1036000.v1.1

Dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000167
Physical Location & Seq
Forward (+)
4725955 .. 4727103
1149 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1036000.v1.1.491

Sequence Viewer

Length: 588 bp
ATGGTGGCCAAGCATTTCGTAGTCCGTATTCCAGACAACCTACCTCTTGATGGCGCTGCTCCTCTCCTATGTGCTGGGGTTACAACTTACAGCCCCTTGAGGTATTTCGGACTTGATAAACCCGGTATGCATGTGGGTGTGGTTGGTGTAGGTGGTATCGGTCACGTTGCCGTGAAGTTTGCAAAGGCTATGGGGGTTAAGGTCACAGTGATCAATACCTCCCCGACTAAGAAAGATGATGCTATTGAAAACCTTGGTGCAGATTCCTTTTTGGTCAGCCGCGAGGAAGGCCAAATGCAGGGTGCCATGGGCACAATGGATGGCATCCTTGACACTGTTTCTGCAGTCCACCCTCTTTTGCCTTTGGTTGGATTATTGAAGTCTCATGGAAAGCTTGTTTTGCTTGGAGCACCAGATAAGCCTCTTCAGCTACCATGTCGTTGGAGGAATGAAGGAGACCCAAAGATGATTGATTTTGCAACTAAGCACAATATAACAGCAAACGTCGAAGTTATACCAGTTGATTATGCAAACACCGCTATGGAGGGTCTTATCAAAGCTAACGTTAGGTGCAAGTTTTGTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

196

Amino Acids

20.79

Weight (kDa)

8.34

Isoelectric Point (pI)

28.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh_C PF02826 34 - 84 8.3e-07 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
ADH_zinc_N PF00107 52 - 145 1e-13 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000167)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G37980 AT4G37980 AT4G37990
fragaria_vesca FvH4_1g19210 FvH4_1g19220 FvH4_1g19240 FvH4_1g19240 FvH4_1g19261 FvH4_1g19262 FvH4_1g19263 FvH4_1g19263 FvH4_1g19265 FvH4_1g19281 FvH4_1g19281 FvH4_7g07240 FvH4_7g07240 FvH4_7g07240
malus_domestica MD01G1042500.v1.1 MD01G1042600.v1.1 MD01G1042800.v1.1 MD01G1042900.v1.1 MD05G1034100.v1.1 MD10G1035900.v1.1 MD10G1036000.v1.1 MD10G1155000.v1.1 MD10G1155100.v1.1 MD10G1155200.v1.1 MD10G1155400.v1.1 MD10G1155700.v1.1 MD10G1155800.v1.1 MD15G1308800.v1.1 MD15G1308900.v1.1
prunus_persica Prupe.6G205600_v2.0.a1 Prupe.6G205700_v2.0.a1 Prupe.6G205800_v2.0.a1 Prupe.6G205900_v2.0.a1 Prupe.6G206300_v2.0.a1 Prupe.6G206800_v2.0.a1 Prupe.6G207100_v2.0.a1 Prupe.6G207200_v2.0.a1 Prupe.6G207300_v2.0.a1 Prupe.6G207400_v2.0.a1 Prupe.6G207500_v2.0.a1 Prupe.6G207600_v2.0.a1 Prupe.6G207700_v2.0.a1 Prupe.6G207900_v2.0.a1
pyrus_communis pycom01g06970 pycom01g06980 pycom01g06990 pycom05g02460 pycom08g00750 pycom10g13410 pycom15g27310 pycom15g27320
rosa_chinensis RchiOBHm_Chr1g0314861 RchiOBHm_Chr1g0345441 RchiOBHm_Chr1g0345451 RchiOBHm_Chr1g0345621 RchiOBHm_Chr1g0345751 RchiOBHm_Chr1g0345861 RchiOBHm_Chr1g0345871 RchiOBHm_Chr1g0345891 RchiOBHm_Chr2g0094851 RchiOBHm_Chr2g0110471 RchiOBHm_Chr2g0110511 RchiOBHm_Chr2g0110531 RchiOBHm_Chr2g0110541 RchiOBHm_Chr2g0110571 RchiOBHm_Chr5g0039381 RchiOBHm_Chr5g0039391 RchiOBHm_Chr6g0270311 RchiOBHm_Chr6g0270331 RchiOBHm_Chr6g0270411 RchiOBHm_Chr6g0270431
rosa_laevigata RLG00000000515 RLG00000013793 RLG00000013797 RLG00000013799 RLG00000016525 RLG00000017808 RLG00000017811 RLG00000017813 RLG00000017816 RLG00000017819 RLG00000017822 RLG00000017824 RLG00000017825 RLG00000028835
rosa_multiflora Rmu_co8225458.1_g000001 Rmu_sc0000389.1_g000006 Rmu_sc0000389.1_g000014 Rmu_sc0000389.1_g000023 Rmu_sc0001292.1_g000012 Rmu_sc0001292.1_g000026 Rmu_sc0002145.1_g000003 Rmu_sc0002329.1_g000033 Rmu_sc0002833.1_g000044 Rmu_sc0004316.1_g000012 Rmu_sc0004439.1_g000003 Rmu_sc0006422.1_g000028 Rmu_sc0009479.1_g000012 Rmu_sc0010489.1_g000009 Rmu_sc0010489.1_g000010 Rmu_sc0017837.1_g000003 Rmu_sc0032320.1_g000001
rosa_roxburghii Rroxscaffold_1G00050770 Rroxscaffold_2G00133110 Rroxscaffold_2G00133140 Rroxscaffold_2G00133200 Rroxscaffold_2G00133270 Rroxscaffold_2G00133290 Rroxscaffold_2G00133300 Rroxscaffold_2G00133350 Rroxscaffold_4G00292490 Rroxscaffold_4G00309020 Rroxscaffold_4G00331740 Rroxscaffold_4G00331800 Rroxscaffold_6G00404470 Rroxscaffold_7G00197950 Rroxscaffold_7G00197990 Rroxscaffold_7G00198020
rosa_rugosa Rorug01G0002500 Rorug02G0047200 Rorug02G0047300 Rorug02G0047300 Rorug02G0047400 Rorug02G0168000 Rorug02G0168000 Rorug02G0168100 Rorug02G0168100 Rorug02G0168400.1 Rorug03G0157600 Rorug03G0157700 Rorug05G0112600 Rorug06G0054600 Rorug06G0054700
rosa_samantha Rh1AG013300 Rh1AG195600 Rh1AG195700 Rh1AG196000 Rh1BG004900 Rh1BG114300 Rh2AG132300 Rh2BG095500 Rh2BG229700 Rh2BG229800 Rh2CG097400 Rh2CG221500 Rh2CG221800 Rh2CG222100 Rh2CG222200 Rh2CG222500 Rh2CG222600 Rh2CG345000 Rh5BG269700 Rh6AG172900 Rh6AG173100 Rh6CG080500 Rh6DG075900 Rh6DG164700 Rh6DG164900 Rh6DG165200 Rh6DG165300
rosa_wichuraiana Rw0G015390 Rw1G000600 Rw1G016130 Rw1G016190 Rw2G007140 Rw2G016970 Rw2G016990 Rw2G017000 Rw3G018910 Rw3G018920 Rw3G018930 Rw5G024830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 302
AccII CGCG 1 cut(s) 282
AciI CCGC 2 cut(s) 280, 537
AclI AACGTT 1 cut(s) 564
AcoI YGGCCR 1 cut(s) 6
AcuI CTGAAG 1 cut(s) 410
AfiI CCNNNNNNNGG 3 cut(s) 50, 298, 368
AgsI TTSAA 2 cut(s) 248, 379
AluBI AGCT 3 cut(s) 394, 430, 560
AluI AGCT 3 cut(s) 394, 430, 560
Alw21I GWGCWC 1 cut(s) 412
Alw26I GTCTC 2 cut(s) 387, 450
AoxI GGCC 2 cut(s) 6, 289
ApeKI GCWGC 1 cut(s) 56
AspLEI GCGC 1 cut(s) 56
AsuC2I CCSGG 1 cut(s) 123
BaeGI GKGCMC 1 cut(s) 314
BalI TGGCCA 1 cut(s) 8
BanI GGYRCC 1 cut(s) 302
Bbv12I GWGCWC 1 cut(s) 412
BbvI GCAGC 1 cut(s) 43
BccI CCATC 2 cut(s) 44, 314
BceAI ACGGC 1 cut(s) 155
BclI TGATCA 1 cut(s) 210
BcnI CCSGG 1 cut(s) 123
BcoDI GTCTC 2 cut(s) 387, 450
BfmI CTRYAG 1 cut(s) 342
BfoI RGCGCY 1 cut(s) 57
BisI GCNGC 2 cut(s) 57, 280
BlsI GCNGC 2 cut(s) 58, 281
Bme1390I CCNGG 1 cut(s) 123
BmiI GGNNCC 1 cut(s) 304
BmrFI CCNGG 1 cut(s) 123
BmsI GCATC 2 cut(s) 229, 333
BpuEI CTTGAG 1 cut(s) 118
BpuMI CCSGG 1 cut(s) 123
BsaI GGTCTC 1 cut(s) 450
BsaJI CCNNGG 2 cut(s) 253, 306
Bsc4I CCNNNNNNNGG 3 cut(s) 50, 298, 368
Bse1I ACTGG 1 cut(s) 518
BseDI CCNNGG 2 cut(s) 253, 306
BseGI GGATG 2 cut(s) 324, 325
BseLI CCNNNNNNNGG 3 cut(s) 50, 298, 368
BseNI ACTGG 1 cut(s) 518
BseRI GAGGAG 1 cut(s) 51
BseSI GKGCMC 1 cut(s) 314
BseXI GCAGC 1 cut(s) 43
BseYI CCCAGC 1 cut(s) 74
BsgI GTGCAG 1 cut(s) 279
Bsh1236I CGCG 1 cut(s) 282
BshFI GGCC 2 cut(s) 8, 291
BshNI GGYRCC 1 cut(s) 302
BsiHKAI GWGCWC 1 cut(s) 412
BsiSI CCGG 1 cut(s) 123
BslI CCNNNNNNNGG 3 cut(s) 50, 298, 368
BsmAI GTCTC 2 cut(s) 387, 450
BsnI GGCC 2 cut(s) 8, 291
Bso31I GGTCTC 1 cut(s) 450
Bsp1286I GDGCHC 2 cut(s) 314, 412
Bsp143I GATC 1 cut(s) 210
Bsp19I CCATGG 1 cut(s) 306
BspACI CCGC 2 cut(s) 280, 537
BspANI GGCC 2 cut(s) 8, 291
BspFNI CGCG 1 cut(s) 282
BspLI GGNNCC 1 cut(s) 304
BspMAI CTGCAG 1 cut(s) 346
BspT107I GGYRCC 1 cut(s) 302
BspTNI GGTCTC 1 cut(s) 450
BsrI ACTGG 1 cut(s) 518
BssECI CCNNGG 2 cut(s) 253, 306
BssMI GATC 1 cut(s) 210
BssT1I CCWWGG 2 cut(s) 253, 306
Bst4CI ACNGT 2 cut(s) 208, 337
Bst6I CTCTTC 1 cut(s) 429
BstDEI CTNAG 2 cut(s) 228, 483
BstDSI CCRYGG 1 cut(s) 306
BstF5I GGATG 2 cut(s) 324, 325
BstFNI CGCG 1 cut(s) 282
BstH2I RGCGCY 1 cut(s) 57
BstHHI GCGC 1 cut(s) 56
BstKTI GATC 1 cut(s) 213
BstMAI GTCTC 2 cut(s) 387, 450
BstMBI GATC 1 cut(s) 210
BstMWI GCNNNNNNNGC 4 cut(s) 288, 400, 427, 536
BstNSI RCATGY 1 cut(s) 134
BstSCI CCNGG 1 cut(s) 121
BstSFI CTRYAG 1 cut(s) 342
BstSLI GKGCMC 1 cut(s) 314
BstUI CGCG 1 cut(s) 282
BstV1I GCAGC 1 cut(s) 43
BstXI CCANNNNNNTGG 1 cut(s) 441
BsuRI GGCC 2 cut(s) 8, 291
BtgI CCRYGG 1 cut(s) 306
BtsCI GGATG 2 cut(s) 324, 325
BtsIMutI CAGTG 2 cut(s) 213, 333
CfoI GCGC 1 cut(s) 56
CviAII CATG 4 cut(s) 131, 307, 386, 435
CviJI RGCY 9 cut(s) 8, 93, 188, 279, 291, 394, 421, 430, 560
CviKI_1 RGCY 9 cut(s) 8, 93, 188, 279, 291, 394, 421, 430, 560
DdeI CTNAG 2 cut(s) 228, 483
DpnI GATC 1 cut(s) 212
DpnII GATC 1 cut(s) 210
EaeI YGGCCR 1 cut(s) 6
Eam1104I CTCTTC 1 cut(s) 429
EarI CTCTTC 1 cut(s) 429
Eco130I CCWWGG 2 cut(s) 253, 306
Eco31I GGTCTC 1 cut(s) 450
Eco57I CTGAAG 1 cut(s) 410
EcoT14I CCWWGG 2 cut(s) 253, 306
EcoT22I ATGCAT 1 cut(s) 132
ErhI CCWWGG 2 cut(s) 253, 306
FaeI CATG 4 cut(s) 134, 310, 389, 438
FatI CATG 4 cut(s) 130, 306, 385, 434
FbaI TGATCA 1 cut(s) 210
Fnu4HI GCNGC 2 cut(s) 57, 280
FokI GGATG 2 cut(s) 311, 332
Fsp4HI GCNGC 2 cut(s) 57, 280
GlaI GCGC 1 cut(s) 55
GluI GCNGC 2 cut(s) 57, 280
GsaI CCCAGC 1 cut(s) 78
HaeII RGCGCY 1 cut(s) 57
HaeIII GGCC 2 cut(s) 8, 291
HapII CCGG 1 cut(s) 123
HhaI GCGC 1 cut(s) 56
Hin1II CATG 4 cut(s) 134, 310, 389, 438
Hin6I GCGC 1 cut(s) 54
HinP1I GCGC 1 cut(s) 54
HindIII AAGCTT 1 cut(s) 392
HinfI GANTC 1 cut(s) 263
HpaII CCGG 1 cut(s) 123
Hpy166II GTNNAC 1 cut(s) 349
Hpy188I TCNGA 1 cut(s) 110
Hpy188III TCNNGA 2 cut(s) 32, 47
Hpy8I GTNNAC 1 cut(s) 349
Hpy99I CGWCG 1 cut(s) 509
HpyAV CCTTC 2 cut(s) 281, 446
HpyCH4III ACNGT 2 cut(s) 208, 337
HpyCH4IV ACGT 3 cut(s) 165, 504, 564
HpyCH4V TGCA 8 cut(s) 130, 182, 260, 298, 344, 479, 530, 573
HpyF10VI GCNNNNNNNGC 4 cut(s) 288, 400, 427, 536
HpyF3I CTNAG 2 cut(s) 228, 483
HpySE526I ACGT 3 cut(s) 165, 504, 564
Hsp92II CATG 4 cut(s) 134, 310, 389, 438
HspAI GCGC 1 cut(s) 54
Ksp22I TGATCA 1 cut(s) 210
Kzo9I GATC 1 cut(s) 210
LmnI GCTCC 2 cut(s) 64, 407
LpnPI CCDG 6 cut(s) 45, 60, 136, 284, 426, 531
Lsp1109I GCAGC 1 cut(s) 43
LweI GCATC 2 cut(s) 229, 333
MaeII ACGT 3 cut(s) 165, 504, 564
MaeIII GTNAC 3 cut(s) 79, 161, 202
MalI GATC 1 cut(s) 212
MboI GATC 1 cut(s) 210
MboII GAAGA 1 cut(s) 416
MhlI GDGCHC 2 cut(s) 314, 412
MlsI TGGCCA 1 cut(s) 8
MluNI TGGCCA 1 cut(s) 8
MmeI TCCRAC 2 cut(s) 349, 422
MnlI CCTC 9 cut(s) 54, 72, 93, 229, 277, 363, 432, 438, 538
Mox20I TGGCCA 1 cut(s) 8
Mph1103I ATGCAT 1 cut(s) 132
MscI TGGCCA 1 cut(s) 8
MseI TTAA 1 cut(s) 198
MslI CAYNNNNRTG 2 cut(s) 135, 539
Msp20I TGGCCA 1 cut(s) 8
MspI CCGG 1 cut(s) 123
MspR9I CCNGG 1 cut(s) 123
MvnI CGCG 1 cut(s) 282
MwoI GCNNNNNNNGC 4 cut(s) 288, 400, 427, 536
NciI CCSGG 1 cut(s) 123
NcoI CCATGG 1 cut(s) 306
NdeII GATC 1 cut(s) 210
NlaIII CATG 4 cut(s) 134, 310, 389, 438
NlaIV GGNNCC 1 cut(s) 304
NmuCI GTSAC 2 cut(s) 161, 202
NsiI ATGCAT 1 cut(s) 132
NspI RCATGY 1 cut(s) 134
PfeI GAWTC 1 cut(s) 263
PkrI GCNGC 2 cut(s) 58, 281
Psp1406I AACGTT 1 cut(s) 564
PspFI CCCAGC 1 cut(s) 74
PspN4I GGNNCC 1 cut(s) 304
PstI CTGCAG 1 cut(s) 346
RseI CAYNNNNRTG 2 cut(s) 135, 539
SaqAI TTAA 1 cut(s) 198
SatI GCNGC 2 cut(s) 57, 280
Sau3AI GATC 1 cut(s) 210
ScrFI CCNGG 1 cut(s) 123
SduI GDGCHC 2 cut(s) 314, 412
SfaNI GCATC 2 cut(s) 229, 333
SfcI CTRYAG 1 cut(s) 342
SmiMI CAYNNNNRTG 2 cut(s) 135, 539
SmlI CTYRAG 1 cut(s) 97
SmoI CTYRAG 1 cut(s) 97
SsiI CCGC 2 cut(s) 280, 537
StyD4I CCNGG 1 cut(s) 121
StyI CCWWGG 2 cut(s) 253, 306
TaaI ACNGT 2 cut(s) 208, 337
TaiI ACGT 3 cut(s) 168, 507, 567
TaqI TCGA 1 cut(s) 507
TaqII GACCGA 1 cut(s) 149
TauI GCSGC 1 cut(s) 282
TfiI GAWTC 1 cut(s) 263
Tru1I TTAA 1 cut(s) 198
Tru9I TTAA 1 cut(s) 198
TscAI CASTG 2 cut(s) 213, 340
TseFI GTSAC 2 cut(s) 161, 202
TseI GCWGC 1 cut(s) 56
Tsp45I GTSAC 2 cut(s) 161, 202
TspDTI ATGAA 1 cut(s) 465
TspGWI ACGGA 1 cut(s) 14
TspRI CASTG 2 cut(s) 213, 340
XceI RCATGY 1 cut(s) 134
XcmI CCANNNNNNNNNTGG 1 cut(s) 313
Zsp2I ATGCAT 1 cut(s) 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.