Dehydrogenase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 374 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 45 |
| AccI | GTMKAC | 2 cut(s) | 11, 1094 |
| AccII | CGCG | 1 cut(s) | 708 |
| AciI | CCGC | 4 cut(s) | 482, 500, 579, 706 |
| AclWI | GGATC | 1 cut(s) | 310 |
| AcoI | YGGCCR | 2 cut(s) | 168, 429 |
| AcsI | RAATTY | 3 cut(s) | 86, 186, 260 |
| AfaI | GTAC | 5 cut(s) | 255, 310, 328, 383, 643 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 45, 166, 499, 794 |
| AgsI | TTSAA | 6 cut(s) | 85, 127, 265, 341, 805, 1090 |
| AjnI | CCWGG | 1 cut(s) | 53 |
| AluBI | AGCT | 3 cut(s) | 820, 856, 914 |
| AluI | AGCT | 3 cut(s) | 820, 856, 914 |
| Alw21I | GWGCWC | 2 cut(s) | 441, 838 |
| Alw26I | GTCTC | 3 cut(s) | 267, 813, 935 |
| AlwI | GGATC | 1 cut(s) | 310 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 724 |
| Ama87I | CYCGRG | 1 cut(s) | 91 |
| AoxI | GGCC | 3 cut(s) | 168, 429, 586 |
| ApeKI | GCWGC | 4 cut(s) | 50, 479, 728, 965 |
| ApoI | RAATTY | 3 cut(s) | 86, 186, 260 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 465 |
| AspLEI | GCGC | 1 cut(s) | 1035 |
| AspS9I | GGNCC | 1 cut(s) | 157 |
| AsuC2I | CCSGG | 2 cut(s) | 210, 549 |
| AsuHPI | GGTGA | 3 cut(s) | 16, 69, 640 |
| AvaI | CYCGRG | 1 cut(s) | 91 |
| AvaII | GGWCC | 1 cut(s) | 157 |
| BaeGI | GKGCMC | 1 cut(s) | 740 |
| BalI | TGGCCA | 2 cut(s) | 170, 431 |
| BanI | GGYRCC | 1 cut(s) | 374 |
| Bbv12I | GWGCWC | 2 cut(s) | 441, 838 |
| BbvI | GCAGC | 4 cut(s) | 62, 491, 715, 977 |
| BccI | CCATC | 3 cut(s) | 383, 740, 820 |
| BceAI | ACGGC | 1 cut(s) | 999 |
| BciT130I | CCWGG | 1 cut(s) | 55 |
| BciVI | GTATCC | 2 cut(s) | 354, 464 |
| BcnI | CCSGG | 2 cut(s) | 210, 549 |
| BcoDI | GTCTC | 3 cut(s) | 267, 813, 935 |
| BfaI | CTAG | 2 cut(s) | 584, 821 |
| BfmI | CTRYAG | 2 cut(s) | 12, 768 |
| BfoI | RGCGCY | 1 cut(s) | 1036 |
| BfuI | GTATCC | 2 cut(s) | 354, 464 |
| BisI | GCNGC | 6 cut(s) | 51, 480, 483, 706, 729, 966 |
| BlsI | GCNGC | 6 cut(s) | 52, 481, 484, 707, 730, 967 |
| BmcAI | AGTACT | 1 cut(s) | 383 |
| Bme1390I | CCNGG | 3 cut(s) | 55, 210, 549 |
| Bme18I | GGWCC | 1 cut(s) | 157 |
| BmeT110I | CYCGRG | 1 cut(s) | 91 |
| BmgT120I | GGNCC | 1 cut(s) | 157 |
| BmiI | GGNNCC | 4 cut(s) | 207, 376, 394, 486 |
| BmrFI | CCNGG | 3 cut(s) | 55, 210, 549 |
| BmsI | GCATC | 2 cut(s) | 281, 466 |
| Bpu10I | CCTNAGC | 1 cut(s) | 609 |
| BpuEI | CTTGAG | 2 cut(s) | 544, 872 |
| BpuMI | CCSGG | 2 cut(s) | 210, 549 |
| Bsa29I | ATCGAT | 2 cut(s) | 1060, 1071 |
| BsaBI | GATNNNNATC | 1 cut(s) | 714 |
| BsaJI | CCNNGG | 3 cut(s) | 54, 732, 1026 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 264, 294 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 45, 166, 499, 794 |
| Bse1I | ACTGG | 1 cut(s) | 860 |
| Bse3DI | GCAATG | 1 cut(s) | 1020 |
| Bse8I | GATNNNNATC | 1 cut(s) | 714 |
| BseBI | CCWGG | 1 cut(s) | 55 |
| BseCI | ATCGAT | 2 cut(s) | 1060, 1071 |
| BseDI | CCNNGG | 3 cut(s) | 54, 732, 1026 |
| BseGI | GGATG | 2 cut(s) | 296, 751 |
| BseJI | GATNNNNATC | 1 cut(s) | 714 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 45, 166, 499, 794 |
| BseMI | GCAATG | 1 cut(s) | 1020 |
| BseNI | ACTGG | 1 cut(s) | 860 |
| BseRI | GAGGAG | 1 cut(s) | 477 |
| BseSI | GKGCMC | 1 cut(s) | 740 |
| BseXI | GCAGC | 4 cut(s) | 62, 491, 715, 977 |
| BseYI | CCCAGC | 1 cut(s) | 914 |
| Bsh1236I | CGCG | 1 cut(s) | 708 |
| BshFI | GGCC | 3 cut(s) | 170, 431, 588 |
| BshNI | GGYRCC | 1 cut(s) | 374 |
| BshVI | ATCGAT | 2 cut(s) | 1060, 1071 |
| BsiHKAI | GWGCWC | 2 cut(s) | 441, 838 |
| BsiHKCI | CYCGRG | 1 cut(s) | 91 |
| BsiSI | CCGG | 2 cut(s) | 210, 548 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 45, 166, 499, 794 |
| BsmAI | GTCTC | 3 cut(s) | 267, 813, 935 |
| BsmBI | CGTCTC | 1 cut(s) | 935 |
| BsnI | GGCC | 3 cut(s) | 170, 431, 588 |
| BsoBI | CYCGRG | 1 cut(s) | 91 |
| Bsp1286I | GDGCHC | 3 cut(s) | 441, 740, 838 |
| Bsp1407I | TGTACA | 1 cut(s) | 308 |
| Bsp143I | GATC | 2 cut(s) | 302, 715 |
| Bsp19I | CCATGG | 2 cut(s) | 732, 1026 |
| BspACI | CCGC | 4 cut(s) | 482, 500, 579, 706 |
| BspANI | GGCC | 3 cut(s) | 170, 431, 588 |
| BspDI | ATCGAT | 2 cut(s) | 1060, 1071 |
| BspFNI | CGCG | 1 cut(s) | 708 |
| BspLI | GGNNCC | 4 cut(s) | 207, 376, 394, 486 |
| BspMAI | CTGCAG | 1 cut(s) | 772 |
| BspPI | GGATC | 1 cut(s) | 310 |
| BspT107I | GGYRCC | 1 cut(s) | 374 |
| BsrDI | GCAATG | 1 cut(s) | 1020 |
| BsrGI | TGTACA | 1 cut(s) | 308 |
| BsrI | ACTGG | 1 cut(s) | 860 |
| BssECI | CCNNGG | 3 cut(s) | 54, 732, 1026 |
| BssMI | GATC | 2 cut(s) | 302, 715 |
| BssT1I | CCWWGG | 2 cut(s) | 732, 1026 |
| Bst2UI | CCWGG | 1 cut(s) | 55 |
| Bst4CI | ACNGT | 3 cut(s) | 140, 634, 763 |
| Bst6I | CTCTTC | 1 cut(s) | 91 |
| BstAUI | TGTACA | 1 cut(s) | 308 |
| BstC8I | GCNNGC | 1 cut(s) | 726 |
| BstDEI | CTNAG | 1 cut(s) | 609 |
| BstDSI | CCRYGG | 2 cut(s) | 732, 1026 |
| BstF5I | GGATG | 2 cut(s) | 296, 751 |
| BstFNI | CGCG | 1 cut(s) | 708 |
| BstH2I | RGCGCY | 1 cut(s) | 1036 |
| BstHHI | GCGC | 1 cut(s) | 1035 |
| BstKTI | GATC | 2 cut(s) | 305, 718 |
| BstMAI | GTCTC | 3 cut(s) | 267, 813, 935 |
| BstMBI | GATC | 2 cut(s) | 302, 715 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 585, 853, 930 |
| BstNI | CCWGG | 1 cut(s) | 55 |
| BstNSI | RCATGY | 1 cut(s) | 560 |
| BstSCI | CCNGG | 3 cut(s) | 53, 208, 547 |
| BstSFI | CTRYAG | 2 cut(s) | 12, 768 |
| BstSLI | GKGCMC | 1 cut(s) | 740 |
| BstUI | CGCG | 1 cut(s) | 708 |
| BstV1I | GCAGC | 4 cut(s) | 62, 491, 715, 977 |
| Bsu15I | ATCGAT | 2 cut(s) | 1060, 1071 |
| BsuI | GTATCC | 2 cut(s) | 354, 464 |
| BsuRI | GGCC | 3 cut(s) | 170, 431, 588 |
| BsuTUI | ATCGAT | 2 cut(s) | 1060, 1071 |
| BtgI | CCRYGG | 2 cut(s) | 732, 1026 |
| BtgZI | GCGATG | 1 cut(s) | 723 |
| BtsCI | GGATG | 2 cut(s) | 296, 751 |
| Cac8I | GCNNGC | 1 cut(s) | 726 |
| CaiI | CAGNNNCTG | 1 cut(s) | 724 |
| CfoI | GCGC | 1 cut(s) | 1035 |
| Cfr13I | GGNCC | 1 cut(s) | 157 |
| ClaI | ATCGAT | 2 cut(s) | 1060, 1071 |
| CseI | GACGC | 1 cut(s) | 952 |
| Csp6I | GTAC | 5 cut(s) | 254, 309, 327, 382, 642 |
| CviQI | GTAC | 5 cut(s) | 254, 309, 327, 382, 642 |
| DdeI | CTNAG | 1 cut(s) | 609 |
| DpnI | GATC | 2 cut(s) | 304, 717 |
| DpnII | GATC | 2 cut(s) | 302, 715 |
| EaeI | YGGCCR | 2 cut(s) | 168, 429 |
| Eam1104I | CTCTTC | 1 cut(s) | 91 |
| EarI | CTCTTC | 1 cut(s) | 91 |
| Eco130I | CCWWGG | 2 cut(s) | 732, 1026 |
| Eco32I | GATATC | 1 cut(s) | 1058 |
| Eco47I | GGWCC | 1 cut(s) | 157 |
| Eco88I | CYCGRG | 1 cut(s) | 91 |
| EcoRII | CCWGG | 1 cut(s) | 53 |
| EcoRV | GATATC | 1 cut(s) | 1058 |
| EcoT14I | CCWWGG | 2 cut(s) | 732, 1026 |
| EcoT22I | ATGCAT | 2 cut(s) | 296, 558 |
| ErhI | CCWWGG | 2 cut(s) | 732, 1026 |
| Esp3I | CGTCTC | 1 cut(s) | 935 |
| FalI | AAGNNNNNCTT | 1 cut(s) | 1082 |
| FauI | CCCGC | 1 cut(s) | 493 |
| FauNDI | CATATG | 1 cut(s) | 403 |
| FblI | GTMKAC | 2 cut(s) | 11, 1094 |
| Fnu4HI | GCNGC | 6 cut(s) | 51, 480, 483, 706, 729, 966 |
| FokI | GGATG | 2 cut(s) | 303, 758 |
| Fsp4HI | GCNGC | 6 cut(s) | 51, 480, 483, 706, 729, 966 |
| FspBI | CTAG | 2 cut(s) | 584, 821 |
| GlaI | GCGC | 1 cut(s) | 1034 |
| GluI | GCNGC | 6 cut(s) | 51, 480, 483, 706, 729, 966 |
| GsaI | CCCAGC | 1 cut(s) | 918 |
| HaeII | RGCGCY | 1 cut(s) | 1036 |
| HaeIII | GGCC | 3 cut(s) | 170, 431, 588 |
| HapII | CCGG | 2 cut(s) | 210, 548 |
| HgaI | GACGC | 1 cut(s) | 952 |
| HhaI | GCGC | 1 cut(s) | 1035 |
| Hin6I | GCGC | 1 cut(s) | 1033 |
| HinP1I | GCGC | 1 cut(s) | 1033 |
| HinfI | GANTC | 2 cut(s) | 59, 689 |
| HpaII | CCGG | 2 cut(s) | 210, 548 |
| HphI | GGTGA | 3 cut(s) | 16, 69, 640 |
| Hpy166II | GTNNAC | 4 cut(s) | 12, 775, 1018, 1095 |
| Hpy188I | TCNGA | 5 cut(s) | 157, 272, 418, 536, 1055 |
| Hpy188III | TCNNGA | 3 cut(s) | 91, 93, 338 |
| Hpy8I | GTNNAC | 4 cut(s) | 12, 775, 1018, 1095 |
| Hpy99I | CGWCG | 1 cut(s) | 881 |
| HpyAV | CCTTC | 5 cut(s) | 91, 476, 894, 931, 1046 |
| HpyCH4III | ACNGT | 3 cut(s) | 140, 634, 763 |
| HpyCH4V | TGCA | 7 cut(s) | 294, 479, 556, 770, 890, 896, 965 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 585, 853, 930 |
| HpyF3I | CTNAG | 1 cut(s) | 609 |
| HspAI | GCGC | 1 cut(s) | 1033 |
| Kzo9I | GATC | 2 cut(s) | 302, 715 |
| LmnI | GCTCC | 4 cut(s) | 246, 490, 833, 1030 |
| Lsp1109I | GCAGC | 4 cut(s) | 62, 491, 715, 977 |
| LweI | GCATC | 2 cut(s) | 281, 466 |
| MaeI | CTAG | 2 cut(s) | 584, 821 |
| MaeIII | GTNAC | 3 cut(s) | 232, 410, 628 |
| MalI | GATC | 2 cut(s) | 304, 717 |
| MboI | GATC | 2 cut(s) | 302, 715 |
| MboII | GAAGA | 4 cut(s) | 108, 674, 725, 916 |
| MfeI | CAATTG | 1 cut(s) | 891 |
| MhlI | GDGCHC | 3 cut(s) | 441, 740, 838 |
| MlsI | TGGCCA | 2 cut(s) | 170, 431 |
| MluCI | AATT | 7 cut(s) | 86, 186, 219, 260, 322, 343, 891 |
| MluNI | TGGCCA | 2 cut(s) | 170, 431 |
| MmeI | TCCRAC | 3 cut(s) | 250, 268, 811 |
| MnlI | CCTC | 7 cut(s) | 170, 232, 401, 498, 655, 789, 988 |
| Mox20I | TGGCCA | 2 cut(s) | 170, 431 |
| Mph1103I | ATGCAT | 2 cut(s) | 296, 558 |
| MroXI | GAANNNNTTC | 1 cut(s) | 465 |
| MscI | TGGCCA | 2 cut(s) | 170, 431 |
| MseI | TTAA | 2 cut(s) | 624, 1041 |
| MslI | CAYNNNNRTG | 4 cut(s) | 425, 561, 681, 1025 |
| Msp20I | TGGCCA | 2 cut(s) | 170, 431 |
| MspA1I | CMGCKG | 1 cut(s) | 482 |
| MspI | CCGG | 2 cut(s) | 210, 548 |
| MspR9I | CCNGG | 3 cut(s) | 55, 210, 549 |
| MunI | CAATTG | 1 cut(s) | 891 |
| MvaI | CCWGG | 1 cut(s) | 55 |
| MvnI | CGCG | 1 cut(s) | 708 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 585, 853, 930 |
| NciI | CCSGG | 2 cut(s) | 210, 549 |
| NcoI | CCATGG | 2 cut(s) | 732, 1026 |
| NdeI | CATATG | 1 cut(s) | 403 |
| NdeII | GATC | 2 cut(s) | 302, 715 |
| NlaIV | GGNNCC | 4 cut(s) | 207, 376, 394, 486 |
| NmuCI | GTSAC | 2 cut(s) | 232, 628 |
| NsiI | ATGCAT | 2 cut(s) | 296, 558 |
| NspI | RCATGY | 1 cut(s) | 560 |
| PaeR7I | CTCGAG | 1 cut(s) | 91 |
| PdmI | GAANNNNTTC | 1 cut(s) | 465 |
| PfeI | GAWTC | 2 cut(s) | 59, 689 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 45 |
| PfoI | TCCNGGA | 1 cut(s) | 208 |
| PkrI | GCNGC | 6 cut(s) | 52, 481, 484, 707, 730, 967 |
| Psp6I | CCWGG | 1 cut(s) | 53 |
| PspFI | CCCAGC | 1 cut(s) | 914 |
| PspGI | CCWGG | 1 cut(s) | 53 |
| PspN4I | GGNNCC | 4 cut(s) | 207, 376, 394, 486 |
| PspPI | GGNCC | 1 cut(s) | 157 |
| PstI | CTGCAG | 1 cut(s) | 772 |
| PstNI | CAGNNNCTG | 1 cut(s) | 724 |
| RsaI | GTAC | 5 cut(s) | 255, 310, 328, 383, 643 |
| RsaNI | GTAC | 5 cut(s) | 254, 309, 327, 382, 642 |
| RseI | CAYNNNNRTG | 4 cut(s) | 425, 561, 681, 1025 |
| SaqAI | TTAA | 2 cut(s) | 624, 1041 |
| SatI | GCNGC | 6 cut(s) | 51, 480, 483, 706, 729, 966 |
| Sau3AI | GATC | 2 cut(s) | 302, 715 |
| Sau96I | GGNCC | 1 cut(s) | 157 |
| ScaI | AGTACT | 1 cut(s) | 383 |
| ScrFI | CCNGG | 3 cut(s) | 55, 210, 549 |
| SduI | GDGCHC | 3 cut(s) | 441, 740, 838 |
| SfaNI | GCATC | 2 cut(s) | 281, 466 |
| SfcI | CTRYAG | 2 cut(s) | 12, 768 |
| Sfr274I | CTCGAG | 1 cut(s) | 91 |
| SinI | GGWCC | 1 cut(s) | 157 |
| SlaI | CTCGAG | 1 cut(s) | 91 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 425, 561, 681, 1025 |
| SmlI | CTYRAG | 3 cut(s) | 91, 523, 851 |
| SmoI | CTYRAG | 3 cut(s) | 91, 523, 851 |
| Sse9I | AATT | 7 cut(s) | 86, 186, 219, 260, 322, 343, 891 |
| SsiI | CCGC | 4 cut(s) | 482, 500, 579, 706 |
| SspMI | CTAG | 2 cut(s) | 584, 821 |
| StyD4I | CCNGG | 3 cut(s) | 53, 208, 547 |
| StyI | CCWWGG | 2 cut(s) | 732, 1026 |
| TaaI | ACNGT | 3 cut(s) | 140, 634, 763 |
| TaqI | TCGA | 3 cut(s) | 92, 1060, 1071 |
| TasI | AATT | 7 cut(s) | 86, 186, 219, 260, 322, 343, 891 |
| TatI | WGTACW | 3 cut(s) | 253, 308, 381 |
| TauI | GCSGC | 2 cut(s) | 485, 708 |
| TfiI | GAWTC | 2 cut(s) | 59, 689 |
| Tru1I | TTAA | 2 cut(s) | 624, 1041 |
| Tru9I | TTAA | 2 cut(s) | 624, 1041 |
| TseFI | GTSAC | 2 cut(s) | 232, 628 |
| TseI | GCWGC | 4 cut(s) | 50, 479, 728, 965 |
| Tsp45I | GTSAC | 2 cut(s) | 232, 628 |
| TspDTI | ATGAA | 4 cut(s) | 51, 231, 726, 950 |
| TspGWI | ACGGA | 3 cut(s) | 251, 440, 828 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 45 |
| VpaK11BI | GGWCC | 1 cut(s) | 157 |
| XapI | RAATTY | 3 cut(s) | 86, 186, 260 |
| XceI | RCATGY | 1 cut(s) | 560 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 739 |
| XhoI | CTCGAG | 1 cut(s) | 91 |
| XmiI | GTMKAC | 2 cut(s) | 11, 1094 |
| XmnI | GAANNNNTTC | 1 cut(s) | 465 |
| XspI | CTAG | 2 cut(s) | 584, 821 |
| ZrmI | AGTACT | 1 cut(s) | 383 |
| Zsp2I | ATGCAT | 2 cut(s) | 296, 558 |