pycom17g03680

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0000309
Physical Location & Seq
Forward (+)
2472916 .. 2479774
6859 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 927 bp
ATGGCATCACAAAACACCCTTTCTCTCTTCACAGTCACTCTCCTCGTCCTCCTTTCTCTCCTCTCCCAACCAACATCGTCAGAAACACACAACCAAACAACTTCACCATTCGAGTTCCTTAACCATCTCAAGGGCTGTCACAAGGGTGACAAAGTCCAAGGCATCCAAGACCTAAAAAAGTACCTTGAAAAATTTGGATATTTGAATAGCTATTCCGATAATGATGACTTCGACGACATCTTGGAGTCGGCGATCAAAACCTACCAAGTCAATTACCACCTCAAGGCTACCGGAACCTTGGATGCCAAAACAGTGTCGAGAATGACGATGCCACGGTGTGGTGTCCCAGATATCATTAATGGTACCACCTCCATGCGATCGGGAAAGAAAAGGCAACCTCATCACCGTGGCGGAAATGGTTCGACTCACACAGTTGCTCATTTTTCTTTCTTAAACGGAAACCCTAAATGGCCTTCGTCTAAGTACCAACTCACCTATGCTTTTGATCAAAGCACCCCAGCTGAGGCTCAGGCCGCAGTTGCACGAGCTTTTGCAACATGGGCAGGAAACACACACTTCTCGTTCAGTCAAGCCCAAAGCTACGAAAACGCGGATCTGAAGATCAGTTTTGGCAGGGGAAATCATGGAGATGGGAGCCCATTTGACGGCCGAGGTGGGACTCTTGCTCATGCATTTCCACCAACCAATGGGAGATTCCACTACGACGCCGATGAGCCGTGGGCTGTGGGGGCTGTGGAGGGTGCTTATGACTTGGAAACCGTGGCTTTGCATGAAATCGGGCATCTGCTTGGGCTAGGACATAGCTCTGTTGAAGGAGCTGTCATGTCTTCCGGTGTTCGTACTGGATTTACCCAAAGTTTGCATGCGGATGATATTCAAGGAATTAAAGCTTTATATAAAACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

309

Amino Acids

33.53

Weight (kDa)

6.59

Isoelectric Point (pI)

28.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 53 - 108 2.4e-11 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 156 - 306 2.5e-46 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 362
AccB1I GGYRCC 1 cut(s) 362
AccB7I CCANNNNNTGG 2 cut(s) 338, 707
AccII CGCG 1 cut(s) 611
AciI CCGC 4 cut(s) 411, 534, 611, 887
AclWI GGATC 1 cut(s) 621
AcoI YGGCCR 1 cut(s) 667
AcsI RAATTY 1 cut(s) 191
AcuI CTGAAG 1 cut(s) 638
AcyI GRCGYC 1 cut(s) 726
AdeI CACNNNGTG 1 cut(s) 338
AfaI GTAC 4 cut(s) 182, 364, 485, 862
AfiI CCNNNNNNNGG 6 cut(s) 130, 283, 338, 523, 665, 707
AgsI TTSAA 4 cut(s) 188, 205, 833, 899
AjuI GAANNNNNNNTTGG 2 cut(s) 698, 730
AleI CACNNNNGTG 1 cut(s) 144
AluBI AGCT 7 cut(s) 210, 521, 548, 600, 825, 839, 911
AluI AGCT 7 cut(s) 210, 521, 548, 600, 825, 839, 911
AlwI GGATC 1 cut(s) 621
AoxI GGCC 3 cut(s) 470, 531, 667
ApoI RAATTY 1 cut(s) 191
AseI ATTAAT 1 cut(s) 357
Asp700I GAANNNNTTC 1 cut(s) 418
Asp718I GGTACC 1 cut(s) 362
AsuHPI GGTGA 4 cut(s) 96, 158, 395, 484
BanI GGYRCC 1 cut(s) 362
BanII GRGCYC 1 cut(s) 659
BauI CACGAG 1 cut(s) 543
BbsI GAAGAC 1 cut(s) 840
BbvCI CCTCAGC 1 cut(s) 522
BccI CCATC 2 cut(s) 132, 644
BceAI ACGGC 2 cut(s) 682, 721
BclI TGATCA 1 cut(s) 505
BfaI CTAG 1 cut(s) 815
BisI GCNGC 1 cut(s) 534
BlsI GCNGC 1 cut(s) 535
BmiI GGNNCC 3 cut(s) 295, 364, 656
BmsI GCATC 5 cut(s) 14, 171, 292, 318, 811
BpiI GAAGAC 1 cut(s) 840
Bpu10I CCTNAGC 2 cut(s) 522, 528
BpuEI CTTGAG 2 cut(s) 113, 266
BsaHI GRCGYC 1 cut(s) 726
BsaJI CCNNGG 7 cut(s) 157, 297, 332, 406, 670, 737, 780
BsaWI WCCGGW 2 cut(s) 290, 851
Bsc4I CCNNNNNNNGG 6 cut(s) 130, 283, 338, 523, 665, 707
Bse1I ACTGG 1 cut(s) 868
BseDI CCNNGG 7 cut(s) 157, 297, 332, 406, 670, 737, 780
BseGI GGATG 3 cut(s) 162, 307, 895
BseLI CCNNNNNNNGG 6 cut(s) 130, 283, 338, 523, 665, 707
BseMII CTCAG 2 cut(s) 513, 542
BseNI ACTGG 1 cut(s) 868
BseRI GAGGAG 2 cut(s) 32, 50
BseX3I CGGCCG 1 cut(s) 667
BseYI CCCAGC 1 cut(s) 517
Bsh1236I CGCG 1 cut(s) 611
Bsh1285I CGRYCG 2 cut(s) 380, 670
BshFI GGCC 3 cut(s) 472, 533, 669
BshNI GGYRCC 1 cut(s) 362
BsiEI CGRYCG 2 cut(s) 380, 670
BsiSI CCGG 2 cut(s) 291, 852
BslFI GGGAC 2 cut(s) 329, 691
BslI CCNNNNNNNGG 6 cut(s) 130, 283, 338, 523, 665, 707
BsmFI GGGAC 2 cut(s) 329, 691
BsnI GGCC 3 cut(s) 472, 533, 669
Bsp1286I GDGCHC 1 cut(s) 659
Bsp143I GATC 5 cut(s) 252, 377, 505, 613, 621
BspACI CCGC 4 cut(s) 411, 534, 611, 887
BspANI GGCC 3 cut(s) 472, 533, 669
BspCNI CTCAG 2 cut(s) 514, 541
BspFNI CGCG 1 cut(s) 611
BspLI GGNNCC 3 cut(s) 295, 364, 656
BspPI GGATC 1 cut(s) 621
BspT107I GGYRCC 1 cut(s) 362
BsrI ACTGG 1 cut(s) 868
BssECI CCNNGG 7 cut(s) 157, 297, 332, 406, 670, 737, 780
BssMI GATC 5 cut(s) 252, 377, 505, 613, 621
BssNI GRCGYC 1 cut(s) 726
BssSI CACGAG 1 cut(s) 543
BssT1I CCWWGG 2 cut(s) 157, 297
Bst2BI CACGAG 1 cut(s) 543
Bst4CI ACNGT 6 cut(s) 34, 313, 336, 407, 433, 781
Bst6I CTCTTC 1 cut(s) 32
BstACI GRCGYC 1 cut(s) 726
BstC8I GCNNGC 1 cut(s) 885
BstDEI CTNAG 3 cut(s) 480, 522, 528
BstDSI CCRYGG 4 cut(s) 332, 406, 737, 780
BstF5I GGATG 3 cut(s) 162, 307, 895
BstFNI CGCG 1 cut(s) 611
BstKTI GATC 5 cut(s) 255, 380, 508, 616, 624
BstMBI GATC 5 cut(s) 252, 377, 505, 613, 621
BstMCI CGRYCG 2 cut(s) 380, 670
BstMWI GCNNNNNNNGC 4 cut(s) 533, 539, 560, 749
BstNSI RCATGY 1 cut(s) 887
BstUI CGCG 1 cut(s) 611
BstV2I GAAGAC 1 cut(s) 840
BstX2I RGATCY 1 cut(s) 613
BstYI RGATCY 1 cut(s) 613
BstZI CGGCCG 1 cut(s) 667
BsuRI GGCC 3 cut(s) 472, 533, 669
BtgI CCRYGG 4 cut(s) 332, 406, 737, 780
BtsCI GGATG 3 cut(s) 162, 307, 895
BtsIMutI CAGTG 1 cut(s) 318
Cac8I GCNNGC 1 cut(s) 885
CseI GACGC 1 cut(s) 734
Csp6I GTAC 4 cut(s) 181, 363, 484, 861
CviAII CATG 7 cut(s) 373, 558, 644, 689, 791, 844, 884
CviQI GTAC 4 cut(s) 181, 363, 484, 861
DdeI CTNAG 3 cut(s) 480, 522, 528
DpnI GATC 5 cut(s) 254, 379, 507, 615, 623
DpnII GATC 5 cut(s) 252, 377, 505, 613, 621
DraIII CACNNNGTG 1 cut(s) 338
EaeI YGGCCR 1 cut(s) 667
EagI CGGCCG 1 cut(s) 667
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
EciI GGCGGA 1 cut(s) 426
EclXI CGGCCG 1 cut(s) 667
Eco130I CCWWGG 2 cut(s) 157, 297
Eco24I GRGCYC 1 cut(s) 659
Eco32I GATATC 1 cut(s) 352
Eco52I CGGCCG 1 cut(s) 667
Eco57I CTGAAG 1 cut(s) 638
EcoRV GATATC 1 cut(s) 352
EcoT14I CCWWGG 2 cut(s) 157, 297
EcoT22I ATGCAT 1 cut(s) 694
EcoT38I GRGCYC 1 cut(s) 659
ErhI CCWWGG 2 cut(s) 157, 297
FaeI CATG 7 cut(s) 376, 561, 647, 692, 794, 847, 887
FaqI GGGAC 2 cut(s) 329, 691
FatI CATG 7 cut(s) 372, 557, 643, 688, 790, 843, 883
FbaI TGATCA 1 cut(s) 505
Fnu4HI GCNGC 1 cut(s) 534
FokI GGATG 3 cut(s) 149, 314, 902
FriOI GRGCYC 1 cut(s) 659
Fsp4HI GCNGC 1 cut(s) 534
FspBI CTAG 1 cut(s) 815
GluI GCNGC 1 cut(s) 534
GsaI CCCAGC 1 cut(s) 521
HaeIII GGCC 3 cut(s) 472, 533, 669
HapII CCGG 2 cut(s) 291, 852
HgaI GACGC 1 cut(s) 734
Hin1I GRCGYC 1 cut(s) 726
Hin1II CATG 7 cut(s) 376, 561, 647, 692, 794, 847, 887
HindIII AAGCTT 1 cut(s) 909
HinfI GANTC 4 cut(s) 245, 424, 679, 714
HpaII CCGG 2 cut(s) 291, 852
HphI GGTGA 4 cut(s) 96, 158, 395, 484
Hpy188I TCNGA 3 cut(s) 82, 217, 618
Hpy188III TCNNGA 2 cut(s) 318, 381
Hpy99I CGWCG 2 cut(s) 236, 728
HpyAV CCTTC 2 cut(s) 483, 827
HpyCH4III ACNGT 6 cut(s) 34, 313, 336, 407, 433, 781
HpyCH4V TGCA 5 cut(s) 542, 554, 692, 790, 883
HpyF10VI GCNNNNNNNGC 4 cut(s) 533, 539, 560, 749
HpyF3I CTNAG 3 cut(s) 480, 522, 528
Hsp92I GRCGYC 1 cut(s) 726
Hsp92II CATG 7 cut(s) 376, 561, 647, 692, 794, 847, 887
KpnI GGTACC 1 cut(s) 366
Ksp22I TGATCA 1 cut(s) 505
Kzo9I GATC 5 cut(s) 252, 377, 505, 613, 621
LmnI GCTCC 2 cut(s) 654, 836
LpnPI CCDG 8 cut(s) 304, 360, 515, 531, 549, 619, 849, 865
LweI GCATC 5 cut(s) 14, 171, 292, 318, 811
MaeI CTAG 1 cut(s) 815
MaeIII GTNAC 3 cut(s) 34, 137, 146
MalI GATC 5 cut(s) 254, 379, 507, 615, 623
MboI GATC 5 cut(s) 252, 377, 505, 613, 621
MboII GAAGA 3 cut(s) 19, 631, 840
MflI RGATCY 1 cut(s) 613
MhlI GDGCHC 1 cut(s) 659
MluCI AATT 3 cut(s) 191, 271, 903
MlyI GAGTC 3 cut(s) 254, 418, 673
MnlI CCTC 9 cut(s) 53, 59, 71, 290, 379, 408, 517, 665, 751
Mph1103I ATGCAT 1 cut(s) 694
MroXI GAANNNNTTC 1 cut(s) 418
MseI TTAA 4 cut(s) 120, 357, 452, 906
MslI CAYNNNNRTG 5 cut(s) 144, 371, 405, 648, 888
MspA1I CMGCKG 1 cut(s) 521
MspI CCGG 2 cut(s) 291, 852
MvnI CGCG 1 cut(s) 611
MwoI GCNNNNNNNGC 4 cut(s) 533, 539, 560, 749
NdeII GATC 5 cut(s) 252, 377, 505, 613, 621
NlaIII CATG 7 cut(s) 376, 561, 647, 692, 794, 847, 887
NlaIV GGNNCC 3 cut(s) 295, 364, 656
NmeAIII GCCGAG 1 cut(s) 695
NmuCI GTSAC 3 cut(s) 34, 137, 146
NsiI ATGCAT 1 cut(s) 694
NspI RCATGY 1 cut(s) 887
OliI CACNNNNGTG 1 cut(s) 144
PaeI GCATGC 1 cut(s) 887
PcsI WCGNNNNNNNCGW 1 cut(s) 323
PdmI GAANNNNTTC 1 cut(s) 418
PfeI GAWTC 1 cut(s) 714
PflFI GACNNNGTC 1 cut(s) 152
PflMI CCANNNNNTGG 2 cut(s) 338, 707
PkrI GCNGC 1 cut(s) 535
Ple19I CGATCG 1 cut(s) 380
PleI GAGTC 3 cut(s) 253, 418, 673
PpsI GAGTC 3 cut(s) 253, 418, 673
PshBI ATTAAT 1 cut(s) 357
PspFI CCCAGC 1 cut(s) 517
PspN4I GGNNCC 3 cut(s) 295, 364, 656
PsuI RGATCY 1 cut(s) 613
PsyI GACNNNGTC 1 cut(s) 152
PvuI CGATCG 1 cut(s) 380
PvuII CAGCTG 1 cut(s) 521
RsaI GTAC 4 cut(s) 182, 364, 485, 862
RsaNI GTAC 4 cut(s) 181, 363, 484, 861
RseI CAYNNNNRTG 5 cut(s) 144, 371, 405, 648, 888
SaqAI TTAA 4 cut(s) 120, 357, 452, 906
SatI GCNGC 1 cut(s) 534
Sau3AI GATC 5 cut(s) 252, 377, 505, 613, 621
SchI GAGTC 3 cut(s) 254, 418, 673
SduI GDGCHC 1 cut(s) 659
SfaNI GCATC 5 cut(s) 14, 171, 292, 318, 811
SmiMI CAYNNNNRTG 5 cut(s) 144, 371, 405, 648, 888
SmlI CTYRAG 2 cut(s) 128, 281
SmoI CTYRAG 2 cut(s) 128, 281
SphI GCATGC 1 cut(s) 887
Sse9I AATT 3 cut(s) 191, 271, 903
SsiI CCGC 4 cut(s) 411, 534, 611, 887
SspMI CTAG 1 cut(s) 815
StyI CCWWGG 2 cut(s) 157, 297
TaaI ACNGT 6 cut(s) 34, 313, 336, 407, 433, 781
TaqI TCGA 4 cut(s) 111, 231, 317, 422
TasI AATT 3 cut(s) 191, 271, 903
TauI GCSGC 1 cut(s) 536
TfiI GAWTC 1 cut(s) 714
Tru1I TTAA 4 cut(s) 120, 357, 452, 906
Tru9I TTAA 4 cut(s) 120, 357, 452, 906
TscAI CASTG 1 cut(s) 318
TseFI GTSAC 3 cut(s) 34, 137, 146
Tsp45I GTSAC 3 cut(s) 34, 137, 146
TspDTI ATGAA 1 cut(s) 807
TspGWI ACGGA 1 cut(s) 471
TspRI CASTG 1 cut(s) 318
Tth111I GACNNNGTC 1 cut(s) 152
Van91I CCANNNNNTGG 2 cut(s) 338, 707
VspI ATTAAT 1 cut(s) 357
XapI RAATTY 1 cut(s) 191
XceI RCATGY 1 cut(s) 887
XmnI GAANNNNTTC 1 cut(s) 418
XspI CTAG 1 cut(s) 815
Zsp2I ATGCAT 1 cut(s) 694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.