pycom17g05440

phenolic glucoside malonyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0000138
Physical Location & Seq
Forward (+)
3864628 .. 3866169
1542 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g05440.1

Sequence Viewer

Length: 1542 bp
ATGGCATTGAAATTCATTCAAGTTTGCAGGGTAGCTCCACAACCAGGCTCGTCGGCCGCCTTGGCCATCCCAGCTCAAGGTGAGAAACTTACGTATGATGTAGATGCCGCTACGTCAGAAGCAATATCTCAAGTCAATCACACTCTACCAGCTCAAGAGGACTTGTCTCTTCCTCTAACCTTCATAGACCTCGTATGGCTAAGGTATCCACCCTTCCATCGCCTTTTCTTCTATGACATATTGCCTTGCTCTTCCCCGGCAGATACCCTACTCTTCTTTCATTCAATAATTATTCCAAAACTCAAAACCTCTCTCTCTATCGCCCTCCAACATTTTCTACCTCTAGCCGGAAACATCACTTGGCCCGAAAGCTCCCCCAAACCCACCCTAAATTATGTCAAAGGCGACACCGTTTCCCTCACAGTAGCCGAATCTGATGCCGATTTCCACGATCTCTCAAGCAATGAATTTAACATTGATGCCAATGAATACCACCCTCTTGTACCCCAACTGGCGAAATCTCACGAAAAAGCCGCCGTGATGGCGTTTCAAATCACCGTCTTTCCCAACAGCCGCGGCTTTTCCATTGGAATATCCATGCACCATGCAGTTGTTGACGGCAAGACTCTCTTCATGTTTCTGAAATCATGGACCCACATATGCAGACATGTTCATAAATCTGATGACATTGTTTTGCCTGATCAGCTCAAACCATTTTACGACAGACGGGGTCTGAAAGACCCCATCTGTCTCCAACTGGAAGAGATTTACTTGAATCAGTTTCTGAACATGGACAGACGACAAAATAATCGAAGCTTGAATGTTGCAGCCCGCTATGAATCTCTAGTGATATCAAATTCAACTCGGGGCAACTTCGAATTCACGAGCGCAAAGATTCAAGCTTTACGGAAATGGGTCAGGACCAGGAAACAACGGGAAGGAGATGATGGATCCGTTCATTTGTCGACATTTTCTCTAACGTGTGCCTACACATGGGTTTGCTTACTAAAAGCAGAGAGAGAAGAGCAAGAAGAAGTAAACGGGGGCCAAATAATACCTATGGCATTTAGCGTGGACTGCCGGTCGCGCTTAACCCCTCCTTTGCCTGGAAACTATTTTGGAAACTGTGTAACGGGCTCTGTAGCACTTGCAGAAAGAAAGGGACTTCTAGGAGAAGACGGGTTGGTTGTAGCGGTAAATGCGATTAGTGAAGCCATTAAGGGTTTGGATGGCGGGACTTTGACGAATGGCGCGGAGAATTTGGTTTCCATATTTTACCCTTCTGATGAACAGACTAGTAGTGGTGCTGGTCATCGGAGAGTATTTACGACCGCTGGATCGCATCGGTTTGACATGTACGATACTGATTTTGGATGGGGAAGGCCAAGGAGCACCGAAGTAGTTCGGATACATACAATCGGGATAATCACTTTTGCAGATGGCAAGAATGGTGGCGGAGCTGTTGACATCGGGTTGGTTTTGAAAAAACATCATATGGAGGCTTTTGCTTCGCTATTTGCTAAAGGTCTTGAAACCCTTTGA

Protein Analysis

514

Amino Acids

56.79

Weight (kDa)

6.32

Isoelectric Point (pI)

37.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 51 - 504 8.6e-52 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000138)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G29590 AT3G29635 AT3G29636 AT3G29670 AT3G29680 AT3G29690 AT3G29720 AT5G39050 AT5G39080 AT5G39090 AT5G61160
fragaria_vesca FvH4_6g46740 FvH4_6g46741 FvH4_6g46742 FvH4_6g46743 FvH4_6g46743 FvH4_6g46750 FvH4_6g46770 FvH4_6g46780 FvH4_6g48750 FvH4_6g48770 FvH4_7g01310 FvH4_7g01410
malus_domestica MD09G1060700.v1.1 MD09G1067500.v1.1 MD09G1067900.v1.1 MD09G1068000.v1.1 MD09G1068100.v1.1 MD09G1080400.v1.1 MD09G1080500.v1.1 MD17G1056000.v1.1 MD17G1056100.v1.1 MD17G1060500.v1.1 MD17G1060600.v1.1 MD17G1060700.v1.1 MD17G1060800.v1.1 MD17G1061000.v1.1 MD17G1070900.v1.1 MD17G1071300.v1.1 MD17G1071400.v1.1
prunus_persica Prupe.3G252700_v2.0.a1 Prupe.3G252800_v2.0.a1 Prupe.3G252900_v2.0.a1 Prupe.3G253000_v2.0.a1 Prupe.3G253100_v2.0.a1 Prupe.3G253200_v2.0.a1 Prupe.3G253300_v2.0.a1 Prupe.3G253400_v2.0.a1 Prupe.3G253500_v2.0.a1 Prupe.3G253600_v2.0.a1 Prupe.3G253700_v2.0.a1 Prupe.3G253800_v2.0.a1 Prupe.3G253900_v2.0.a1 Prupe.3G254000_v2.0.a1 Prupe.3G254200_v2.0.a1 Prupe.3G254300_v2.0.a1 Prupe.3G254400_v2.0.a1
pyrus_communis pycom09g00710 pycom111g05670 pycom111g05680 pycom111g05700 pycom111g05710 pycom111g05720 pycom12433g00160 pycom17g05440 pycom17g05520 pycom17g06020 pycom17g06030 pycom17g06040 pycom17g06050 pycom17g06060 pycom17g07080
rosa_chinensis RchiOBHm_Chr1g0317141 RchiOBHm_Chr1g0317151 RchiOBHm_Chr1g0317191 RchiOBHm_Chr1g0317201 RchiOBHm_Chr1g0317211 RchiOBHm_Chr1g0334681 RchiOBHm_Chr1g0334691 RchiOBHm_Chr2g0165681 RchiOBHm_Chr2g0165721 RchiOBHm_Chr2g0165731 RchiOBHm_Chr2g0165741 RchiOBHm_Chr2g0165751 RchiOBHm_Chr2g0165771 RchiOBHm_Chr2g0165781 RchiOBHm_Chr5g0029321 RchiOBHm_Chr5g0029331
rosa_laevigata RLG00000021545 RLG00000021546 RLG00000021548 RLG00000021549 RLG00000021550 RLG00000021551 RLG00000021552 RLG00000029433 RLG00000029434 RLG00000029435 RLG00000029439 RLG00000029441 RLG00000029442 RLG00000030618 RLG00000030619 RLG00000030620 RLG00000030622 RLG00000033153 RLG00000033154 RLG00000033155 RLG00000033156
rosa_multiflora Rmu_co7981466.1_g000001 Rmu_co8034280.1_g000001 Rmu_co8243107.1_g000001 Rmu_co8406929.1_g000001 Rmu_sc0000802.1_g000001 Rmu_sc0002295.1_g000005 Rmu_sc0003227.1_g000026 Rmu_sc0003227.1_g000028 Rmu_sc0003227.1_g000029 Rmu_sc0003227.1_g000030 Rmu_sc0003227.1_g000031 Rmu_sc0003689.1_g000001 Rmu_sc0003689.1_g000007 Rmu_sc0004137.1_g000001 Rmu_sc0004205.1_g000004 Rmu_sc0006595.1_g000001 Rmu_sc0006595.1_g000002 Rmu_sc0006595.1_g000003 Rmu_sc0006595.1_g000005 Rmu_sc0009268.1_g000003 Rmu_sc0009268.1_g000004 Rmu_sc0009268.1_g000005 Rmu_sc0009268.1_g000012 Rmu_sc0010198.1_g000001 Rmu_sc0010198.1_g000003 Rmu_sc0010463.1_g000008 Rmu_sc0011453.1_g000001 Rmu_sc0013848.1_g000001 Rmu_sc0013964.1_g000002 Rmu_sc0020734.1_g000001 Rmu_sc0021275.1_g000001 Rmu_sc0024807.1_g000001
rosa_roxburghii Rroxscaffold_1G00039060 Rroxscaffold_2G00085550 Rroxscaffold_2G00085570 Rroxscaffold_2G00085590 Rroxscaffold_2G00085600 Rroxscaffold_2G00085630 Rroxscaffold_2G00085660 Rroxscaffold_2G00085670 Rroxscaffold_4G00316670 Rroxscaffold_4G00316720 Rroxscaffold_4G00316730 Rroxscaffold_4G00330570 Rroxscaffold_4G00330590 Rroxscaffold_4G00330600 Rroxscaffold_4G00330620
rosa_rugosa Rorug01G0014900 Rorug01G0015000 Rorug01G0015200 Rorug01G0114800 Rorug01G0114800 Rorug02G0516600 Rorug02G0516700 Rorug02G0516800 Rorug02G0516900 Rorug02G0517000 Rorug02G0517100 Rorug02G0517200 Rorug02G0517300 Rorug05G0113300
rosa_samantha Rh1BG022500 Rh1BG022600 Rh1BG106200 Rh1BG106400 Rh1BG106500 Rh2AG557600 Rh2CG564900 Rh2CG565000 Rh2CG565100 Rh2CG565200 Rh2CG565400 Rh2CG565500 Rh2CG565600 Rh2CG565700 Rh5CG225500 Rh5CG225600
rosa_wichuraiana Rw0G011340 Rw0G011350 Rw0G011360 Rw0G016000 Rw0G016010 Rw0G016020 Rw0G016030 Rw1G001820 Rw1G001830 Rw1G011540 Rw1G011550 Rw2G048610 Rw2G048620 Rw2G048630 Rw5G018670 Rw5G018680 Rw5G018690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 965
AccII CGCG 3 cut(s) 576, 1087, 1253
AclWI GGATC 3 cut(s) 945, 958, 1345
AcoI YGGCCR 2 cut(s) 54, 63
AcsI RAATTY 5 cut(s) 11, 467, 856, 878, 1258
AfaI GTAC 2 cut(s) 504, 1358
AfiI CCNNNNNNNGG 5 cut(s) 44, 77, 347, 993, 1106
AflIII ACRYGT 3 cut(s) 667, 980, 1353
AhdI GACNNNNNGTC 1 cut(s) 1081
AhlI ACTAGT 1 cut(s) 1295
AjnI CCWGG 3 cut(s) 43, 923, 1105
AjuI GAANNNNNNNTTGG 2 cut(s) 343, 375
AluBI AGCT 8 cut(s) 35, 74, 152, 372, 706, 816, 902, 1460
AluI AGCT 8 cut(s) 35, 74, 152, 372, 706, 816, 902, 1460
Alw21I GWGCWC 1 cut(s) 1394
Alw26I GTCTC 2 cut(s) 171, 755
AlwI GGATC 3 cut(s) 945, 958, 1345
AlwNI CAGNNNCTG 1 cut(s) 784
Ama87I CYCGRG 1 cut(s) 864
AoxI GGCC 5 cut(s) 54, 63, 362, 1045, 1382
ApeKI GCWGC 1 cut(s) 827
ApoI RAATTY 5 cut(s) 11, 467, 856, 878, 1258
Asp700I GAANNNNTTC 1 cut(s) 1401
AspLEI GCGC 3 cut(s) 890, 1089, 1253
AspS9I GGNCC 4 cut(s) 363, 651, 921, 1045
AsuC2I CCSGG 1 cut(s) 257
AsuHPI GGTGA 2 cut(s) 92, 547
AsuII TTCGAA 1 cut(s) 876
AvaI CYCGRG 1 cut(s) 864
AvaII GGWCC 2 cut(s) 651, 921
BalI TGGCCA 1 cut(s) 65
BamHI GGATCC 1 cut(s) 950
BanII GRGCYC 1 cut(s) 1139
BarI GAAGNNNNNNTAC 2 cut(s) 197, 229
BauI CACGAG 1 cut(s) 883
BbsI GAAGAC 1 cut(s) 1182
Bbv12I GWGCWC 1 cut(s) 1394
BbvI GCAGC 1 cut(s) 839
BccI CCATC 8 cut(s) 74, 225, 535, 752, 941, 1223, 1368, 1433
BceAI ACGGC 2 cut(s) 521, 634
BcgI CGANNNNNNTGC 2 cut(s) 419, 453
BciT130I CCWGG 3 cut(s) 45, 925, 1107
BciVI GTATCC 2 cut(s) 216, 1401
BclI TGATCA 1 cut(s) 700
BcnI CCSGG 1 cut(s) 257
BcoDI GTCTC 2 cut(s) 171, 755
BcuI ACTAGT 1 cut(s) 1295
BfaI CTAG 4 cut(s) 344, 845, 1169, 1296
BfmI CTRYAG 1 cut(s) 1140
BfuI GTATCC 2 cut(s) 216, 1401
BglI GCCNNNNNGGC 2 cut(s) 62, 542
BisI GCNGC 6 cut(s) 57, 108, 534, 574, 577, 828
BlsI GCNGC 6 cut(s) 58, 109, 535, 575, 578, 829
Bme1390I CCNGG 4 cut(s) 45, 257, 925, 1107
Bme18I GGWCC 2 cut(s) 651, 921
BmeRI GACNNNNNGTC 1 cut(s) 1081
BmeT110I CYCGRG 1 cut(s) 864
BmgT120I GGNCC 4 cut(s) 363, 651, 921, 1045
BmiI GGNNCC 3 cut(s) 653, 952, 1046
BmrFI CCNGG 4 cut(s) 45, 257, 925, 1107
BmsI GCATC 4 cut(s) 94, 427, 469, 1351
BpiI GAAGAC 1 cut(s) 1182
Bpu10I CCTNAGC 1 cut(s) 200
Bpu14I TTCGAA 1 cut(s) 876
BpuEI CTTGAG 4 cut(s) 60, 114, 138, 442
BpuMI CCSGG 1 cut(s) 257
BsaAI YACGTR 1 cut(s) 93
BsaJI CCNNGG 4 cut(s) 60, 255, 574, 1385
Bsc4I CCNNNNNNNGG 5 cut(s) 44, 77, 347, 993, 1106
Bse118I RCCGGY 1 cut(s) 1080
Bse1I ACTGG 2 cut(s) 516, 762
Bse3DI GCAATG 1 cut(s) 469
BseBI CCWGG 3 cut(s) 45, 925, 1107
BseDI CCNNGG 4 cut(s) 60, 255, 574, 1385
BseGI GGATG 3 cut(s) 66, 1234, 1379
BseLI CCNNNNNNNGG 5 cut(s) 44, 77, 347, 993, 1106
BseMI GCAATG 1 cut(s) 469
BseNI ACTGG 2 cut(s) 516, 762
BseX3I CGGCCG 1 cut(s) 54
BseXI GCAGC 1 cut(s) 839
BseYI CCCAGC 1 cut(s) 70
Bsh1236I CGCG 3 cut(s) 576, 1087, 1253
Bsh1285I CGRYCG 3 cut(s) 57, 1085, 1332
BshFI GGCC 5 cut(s) 56, 65, 364, 1047, 1384
BsiEI CGRYCG 3 cut(s) 57, 1085, 1332
BsiHKAI GWGCWC 1 cut(s) 1394
BsiHKCI CYCGRG 1 cut(s) 864
BsiSI CCGG 3 cut(s) 257, 348, 1081
BslFI GGGAC 2 cut(s) 1176, 1249
BslI CCNNNNNNNGG 5 cut(s) 44, 77, 347, 993, 1106
BsmAI GTCTC 2 cut(s) 171, 755
BsmFI GGGAC 2 cut(s) 1176, 1249
BsnI GGCC 5 cut(s) 56, 65, 364, 1047, 1384
BsoBI CYCGRG 1 cut(s) 864
Bsp119I TTCGAA 1 cut(s) 876
Bsp1286I GDGCHC 2 cut(s) 1139, 1394
Bsp143I GATC 4 cut(s) 451, 700, 950, 1337
BspANI GGCC 5 cut(s) 56, 65, 364, 1047, 1384
BspFNI CGCG 3 cut(s) 576, 1087, 1253
BspLI GGNNCC 3 cut(s) 653, 952, 1046
BspPI GGATC 3 cut(s) 945, 958, 1345
BspQI GCTCTTC 2 cut(s) 256, 1017
BspT104I TTCGAA 1 cut(s) 876
BsrDI GCAATG 1 cut(s) 469
BsrFI RCCGGY 1 cut(s) 1080
BsrI ACTGG 2 cut(s) 516, 762
BssAI RCCGGY 1 cut(s) 1080
BssECI CCNNGG 4 cut(s) 60, 255, 574, 1385
BssMI GATC 4 cut(s) 451, 700, 950, 1337
BssSI CACGAG 1 cut(s) 883
BssT1I CCWWGG 2 cut(s) 60, 1385
Bst2BI CACGAG 1 cut(s) 883
Bst2UI CCWGG 3 cut(s) 45, 925, 1107
Bst4CI ACNGT 4 cut(s) 412, 424, 559, 1127
Bst6I CTCTTC 6 cut(s) 174, 256, 278, 635, 756, 1017
BstBAI YACGTR 1 cut(s) 93
BstBI TTCGAA 1 cut(s) 876
BstC8I GCNNGC 1 cut(s) 832
BstDEI CTNAG 1 cut(s) 200
BstDSI CCRYGG 1 cut(s) 574
BstF5I GGATG 3 cut(s) 66, 1234, 1379
BstFNI CGCG 3 cut(s) 576, 1087, 1253
BstHHI GCGC 3 cut(s) 890, 1089, 1253
BstKTI GATC 4 cut(s) 454, 703, 953, 1340
BstMAI GTCTC 2 cut(s) 171, 755
BstMBI GATC 4 cut(s) 451, 700, 950, 1337
BstMCI CGRYCG 3 cut(s) 57, 1085, 1332
BstMWI GCNNNNNNNGC 7 cut(s) 62, 71, 542, 703, 1077, 1086, 1199
BstNI CCWGG 3 cut(s) 45, 925, 1107
BstNSI RCATGY 2 cut(s) 671, 1357
BstSCI CCNGG 4 cut(s) 43, 255, 923, 1105
BstSFI CTRYAG 1 cut(s) 1140
BstSNI TACGTA 1 cut(s) 93
BstUI CGCG 3 cut(s) 576, 1087, 1253
BstV1I GCAGC 1 cut(s) 839
BstV2I GAAGAC 1 cut(s) 1182
BstX2I RGATCY 1 cut(s) 950
BstYI RGATCY 1 cut(s) 950
BstZI CGGCCG 1 cut(s) 54
BsuI GTATCC 2 cut(s) 216, 1401
BsuRI GGCC 5 cut(s) 56, 65, 364, 1047, 1384
BtgI CCRYGG 1 cut(s) 574
BtgZI GCGATG 1 cut(s) 203
BtsCI GGATG 3 cut(s) 66, 1234, 1379
Cac8I GCNNGC 1 cut(s) 832
CaiI CAGNNNCTG 1 cut(s) 784
CfoI GCGC 3 cut(s) 890, 1089, 1253
Cfr10I RCCGGY 1 cut(s) 1080
Cfr13I GGNCC 4 cut(s) 363, 651, 921, 1045
Cfr42I CCGCGG 1 cut(s) 577
Csp6I GTAC 2 cut(s) 503, 1357
CspCI CAANNNNNGTGG 2 cut(s) 1432, 1467
CviAII CATG 8 cut(s) 598, 605, 634, 648, 668, 790, 993, 1354
CviQI GTAC 2 cut(s) 503, 1357
DdeI CTNAG 1 cut(s) 200
DpnI GATC 4 cut(s) 453, 702, 952, 1339
DpnII GATC 4 cut(s) 451, 700, 950, 1337
DriI GACNNNNNGTC 1 cut(s) 1081
EaeI YGGCCR 2 cut(s) 54, 63
EagI CGGCCG 1 cut(s) 54
Eam1104I CTCTTC 6 cut(s) 174, 256, 278, 635, 756, 1017
Eam1105I GACNNNNNGTC 1 cut(s) 1081
EarI CTCTTC 6 cut(s) 174, 256, 278, 635, 756, 1017
EciI GGCGGA 1 cut(s) 1470
EclXI CGGCCG 1 cut(s) 54
Eco105I TACGTA 1 cut(s) 93
Eco130I CCWWGG 2 cut(s) 60, 1385
Eco24I GRGCYC 1 cut(s) 1139
Eco32I GATATC 1 cut(s) 852
Eco47I GGWCC 2 cut(s) 651, 921
Eco52I CGGCCG 1 cut(s) 54
Eco88I CYCGRG 1 cut(s) 864
EcoRI GAATTC 1 cut(s) 878
EcoRII CCWGG 3 cut(s) 43, 923, 1105
EcoRV GATATC 1 cut(s) 852
EcoT14I CCWWGG 2 cut(s) 60, 1385
EcoT38I GRGCYC 1 cut(s) 1139
ErhI CCWWGG 2 cut(s) 60, 1385
FaeI CATG 8 cut(s) 601, 608, 637, 651, 671, 793, 996, 1357
FalI AAGNNNNNCTT 2 cut(s) 614, 646
FaqI GGGAC 2 cut(s) 1176, 1249
FatI CATG 8 cut(s) 597, 604, 633, 647, 667, 789, 992, 1353
FauI CCCGC 2 cut(s) 839, 1226
FauNDI CATATG 2 cut(s) 659, 1494
FbaI TGATCA 1 cut(s) 700
FblI GTMKAC 1 cut(s) 965
Fnu4HI GCNGC 6 cut(s) 57, 108, 534, 574, 577, 828
FokI GGATG 3 cut(s) 53, 1241, 1386
FriOI GRGCYC 1 cut(s) 1139
Fsp4HI GCNGC 6 cut(s) 57, 108, 534, 574, 577, 828
FspBI CTAG 4 cut(s) 344, 845, 1169, 1296
GlaI GCGC 3 cut(s) 889, 1088, 1252
GluI GCNGC 6 cut(s) 57, 108, 534, 574, 577, 828
GsaI CCCAGC 1 cut(s) 74
HaeIII GGCC 5 cut(s) 56, 65, 364, 1047, 1384
HapII CCGG 3 cut(s) 257, 348, 1081
HhaI GCGC 3 cut(s) 890, 1089, 1253
Hin1II CATG 8 cut(s) 601, 608, 637, 651, 671, 793, 996, 1357
Hin6I GCGC 3 cut(s) 888, 1087, 1251
HinP1I GCGC 3 cut(s) 888, 1087, 1251
HincII GTYRAC 3 cut(s) 616, 966, 1465
HindII GTYRAC 3 cut(s) 616, 966, 1465
HindIII AAGCTT 2 cut(s) 814, 900
HinfI GANTC 5 cut(s) 431, 625, 775, 839, 895
HpaII CCGG 3 cut(s) 257, 348, 1081
HphI GGTGA 2 cut(s) 92, 547
Hpy166II GTNNAC 5 cut(s) 616, 966, 1039, 1075, 1465
Hpy188I TCNGA 9 cut(s) 118, 436, 642, 682, 735, 786, 1285, 1317, 1407
Hpy188III TCNNGA 6 cut(s) 155, 524, 883, 919, 1420, 1529
Hpy8I GTNNAC 5 cut(s) 616, 966, 1039, 1075, 1465
Hpy99I CGWCG 1 cut(s) 55
HpyAV CCTTC 5 cut(s) 190, 223, 932, 1290, 1374
HpyCH4III ACNGT 4 cut(s) 412, 424, 559, 1127
HpyCH4IV ACGT 3 cut(s) 92, 113, 980
HpyCH4V TGCA 7 cut(s) 27, 601, 608, 663, 827, 1151, 1436
HpyF10VI GCNNNNNNNGC 7 cut(s) 62, 71, 542, 703, 1077, 1086, 1199
HpyF3I CTNAG 1 cut(s) 200
HpySE526I ACGT 3 cut(s) 92, 113, 980
Hsp92II CATG 8 cut(s) 601, 608, 637, 651, 671, 793, 996, 1357
HspAI GCGC 3 cut(s) 888, 1087, 1251
Ksp22I TGATCA 1 cut(s) 700
KspI CCGCGG 1 cut(s) 577
Kzo9I GATC 4 cut(s) 451, 700, 950, 1337
LguI GCTCTTC 2 cut(s) 256, 1017
LmnI GCTCC 4 cut(s) 40, 377, 1389, 1457
Lsp1109I GCAGC 1 cut(s) 839
LweI GCATC 4 cut(s) 94, 427, 469, 1351
MaeI CTAG 4 cut(s) 344, 845, 1169, 1296
MaeII ACGT 3 cut(s) 92, 113, 980
MaeIII GTNAC 1 cut(s) 1129
MalI GATC 4 cut(s) 453, 702, 952, 1339
MboI GATC 4 cut(s) 451, 700, 950, 1337
MboII GAAGA 9 cut(s) 161, 220, 243, 265, 622, 773, 1034, 1043, 1187
MflI RGATCY 1 cut(s) 950
MhlI GDGCHC 2 cut(s) 1139, 1394
MlsI TGGCCA 1 cut(s) 65
MluCI AATT 7 cut(s) 11, 288, 391, 467, 856, 878, 1258
MluNI TGGCCA 1 cut(s) 65
MlyI GAGTC 1 cut(s) 619
MmeI TCCRAC 2 cut(s) 352, 778
Mox20I TGGCCA 1 cut(s) 65
MroXI GAANNNNTTC 1 cut(s) 1401
MscI TGGCCA 1 cut(s) 65
MseI TTAA 3 cut(s) 471, 1091, 1218
Msp20I TGGCCA 1 cut(s) 65
MspA1I CMGCKG 2 cut(s) 576, 1334
MspI CCGG 3 cut(s) 257, 348, 1081
MspR9I CCNGG 4 cut(s) 45, 257, 925, 1107
MvaI CCWGG 3 cut(s) 45, 925, 1107
MvnI CGCG 3 cut(s) 576, 1087, 1253
MwoI GCNNNNNNNGC 7 cut(s) 62, 71, 542, 703, 1077, 1086, 1199
NciI CCSGG 1 cut(s) 257
NdeI CATATG 2 cut(s) 659, 1494
NdeII GATC 4 cut(s) 451, 700, 950, 1337
NlaIII CATG 8 cut(s) 601, 608, 637, 651, 671, 793, 996, 1357
NlaIV GGNNCC 3 cut(s) 653, 952, 1046
NspI RCATGY 2 cut(s) 671, 1357
NspV TTCGAA 1 cut(s) 876
PciI ACATGT 2 cut(s) 667, 1353
PciSI GCTCTTC 2 cut(s) 256, 1017
PdmI GAANNNNTTC 1 cut(s) 1401
PfeI GAWTC 4 cut(s) 431, 775, 839, 895
PflFI GACNNNGTC 1 cut(s) 729
PkrI GCNGC 6 cut(s) 58, 109, 535, 575, 578, 829
PleI GAGTC 1 cut(s) 619
PpsI GAGTC 1 cut(s) 619
Ppu21I YACGTR 1 cut(s) 93
PscI ACATGT 2 cut(s) 667, 1353
Psp6I CCWGG 3 cut(s) 43, 923, 1105
PspFI CCCAGC 1 cut(s) 70
PspGI CCWGG 3 cut(s) 43, 923, 1105
PspN4I GGNNCC 3 cut(s) 653, 952, 1046
PspPI GGNCC 4 cut(s) 363, 651, 921, 1045
PstNI CAGNNNCTG 1 cut(s) 784
PsuI RGATCY 1 cut(s) 950
PsyI GACNNNGTC 1 cut(s) 729
RsaI GTAC 2 cut(s) 504, 1358
RsaNI GTAC 2 cut(s) 503, 1357
SacII CCGCGG 1 cut(s) 577
SalI GTCGAC 1 cut(s) 964
SapI GCTCTTC 2 cut(s) 256, 1017
SaqAI TTAA 3 cut(s) 471, 1091, 1218
SatI GCNGC 6 cut(s) 57, 108, 534, 574, 577, 828
Sau3AI GATC 4 cut(s) 451, 700, 950, 1337
Sau96I GGNCC 4 cut(s) 363, 651, 921, 1045
SchI GAGTC 1 cut(s) 619
ScrFI CCNGG 4 cut(s) 45, 257, 925, 1107
SduI GDGCHC 2 cut(s) 1139, 1394
SfaNI GCATC 4 cut(s) 94, 427, 469, 1351
SfcI CTRYAG 1 cut(s) 1140
SfiI GGCCNNNNNGGCC 1 cut(s) 62
Sfr303I CCGCGG 1 cut(s) 577
SfuI TTCGAA 1 cut(s) 876
SgrBI CCGCGG 1 cut(s) 577
SinI GGWCC 2 cut(s) 651, 921
SmlI CTYRAG 4 cut(s) 75, 129, 153, 457
SmoI CTYRAG 4 cut(s) 75, 129, 153, 457
SnaBI TACGTA 1 cut(s) 93
SpeI ACTAGT 1 cut(s) 1295
Sse9I AATT 7 cut(s) 11, 288, 391, 467, 856, 878, 1258
SspMI CTAG 4 cut(s) 344, 845, 1169, 1296
StyD4I CCNGG 4 cut(s) 43, 255, 923, 1105
StyI CCWWGG 2 cut(s) 60, 1385
TaaI ACNGT 4 cut(s) 412, 424, 559, 1127
TaiI ACGT 3 cut(s) 95, 116, 983
TaqI TCGA 3 cut(s) 811, 876, 965
TasI AATT 7 cut(s) 11, 288, 391, 467, 856, 878, 1258
TauI GCSGC 5 cut(s) 59, 110, 536, 576, 579
TfiI GAWTC 4 cut(s) 431, 775, 839, 895
Tru1I TTAA 3 cut(s) 471, 1091, 1218
Tru9I TTAA 3 cut(s) 471, 1091, 1218
TseI GCWGC 1 cut(s) 827
TspGWI ACGGA 2 cut(s) 922, 943
Tth111I GACNNNGTC 1 cut(s) 729
VpaK11BI GGWCC 2 cut(s) 651, 921
XapI RAATTY 5 cut(s) 11, 467, 856, 878, 1258
XceI RCATGY 2 cut(s) 671, 1357
XcmI CCANNNNNNNNNTGG 1 cut(s) 1222
XmiI GTMKAC 1 cut(s) 965
XmnI GAANNNNTTC 1 cut(s) 1401
XspI CTAG 4 cut(s) 344, 845, 1169, 1296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.