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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Alw21I | GWGCWC | 1 cut(s) | 74 |
| Bbv12I | GWGCWC | 1 cut(s) | 74 |
| BsiHKAI | GWGCWC | 1 cut(s) | 74 |
| Bsp1286I | GDGCHC | 1 cut(s) | 74 |
| Bsp143I | GATC | 1 cut(s) | 124 |
| BssMI | GATC | 1 cut(s) | 124 |
| Bst4CI | ACNGT | 3 cut(s) | 36, 121, 151 |
| BstC8I | GCNNGC | 1 cut(s) | 70 |
| BstKTI | GATC | 1 cut(s) | 127 |
| BstMBI | GATC | 1 cut(s) | 124 |
| BtsIMutI | CAGTG | 1 cut(s) | 32 |
| Cac8I | GCNNGC | 1 cut(s) | 70 |
| DpnI | GATC | 1 cut(s) | 126 |
| DpnII | GATC | 1 cut(s) | 124 |
| FaiI | YATR | 4 cut(s) | 90, 111, 130, 135 |
| FspEI | CC | 5 cut(s) | 39, 44, 45, 69, 88 |
| HinfI | GANTC | 1 cut(s) | 80 |
| Hpy188I | TCNGA | 1 cut(s) | 42 |
| Hpy188III | TCNNGA | 2 cut(s) | 16, 84 |
| HpyCH4III | ACNGT | 3 cut(s) | 36, 121, 151 |
| HpyCH4V | TGCA | 1 cut(s) | 4 |
| Kzo9I | GATC | 1 cut(s) | 124 |
| LpnPI | CCDG | 1 cut(s) | 69 |
| MaeIII | GTNAC | 1 cut(s) | 115 |
| MalI | GATC | 1 cut(s) | 126 |
| MboI | GATC | 1 cut(s) | 124 |
| MhlI | GDGCHC | 1 cut(s) | 74 |
| MnlI | CCTC | 1 cut(s) | 96 |
| NdeII | GATC | 1 cut(s) | 124 |
| PfeI | GAWTC | 1 cut(s) | 80 |
| Sau3AI | GATC | 1 cut(s) | 124 |
| SduI | GDGCHC | 1 cut(s) | 74 |
| SgeI | CNNG | 3 cut(s) | 28, 81, 96 |
| TaaI | ACNGT | 3 cut(s) | 36, 121, 151 |
| TaqI | TCGA | 1 cut(s) | 97 |
| TfiI | GAWTC | 1 cut(s) | 80 |
| TscAI | CASTG | 1 cut(s) | 39 |
| TspRI | CASTG | 1 cut(s) | 39 |