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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 32 |
| AclWI | GGATC | 3 cut(s) | 292, 386, 399 |
| AcsI | RAATTY | 1 cut(s) | 373 |
| AcuI | CTGAAG | 2 cut(s) | 213, 300 |
| AgsI | TTSAA | 2 cut(s) | 127, 317 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 294 |
| AjnI | CCWGG | 2 cut(s) | 39, 394 |
| AluBI | AGCT | 1 cut(s) | 143 |
| AluI | AGCT | 1 cut(s) | 143 |
| AlwI | GGATC | 3 cut(s) | 292, 386, 399 |
| ApeKI | GCWGC | 1 cut(s) | 140 |
| ApoI | RAATTY | 1 cut(s) | 373 |
| AspS9I | GGNCC | 1 cut(s) | 266 |
| AsuHPI | GGTGA | 1 cut(s) | 218 |
| AvaII | GGWCC | 1 cut(s) | 266 |
| BamHI | GGATCC | 1 cut(s) | 391 |
| BbsI | GAAGAC | 1 cut(s) | 354 |
| BbvI | GCAGC | 1 cut(s) | 152 |
| BccI | CCATC | 1 cut(s) | 130 |
| BciT130I | CCWGG | 2 cut(s) | 41, 396 |
| BisI | GCNGC | 2 cut(s) | 32, 141 |
| BlsI | GCNGC | 2 cut(s) | 33, 142 |
| Bme1390I | CCNGG | 2 cut(s) | 41, 396 |
| Bme18I | GGWCC | 1 cut(s) | 266 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 294 |
| BmgT120I | GGNCC | 1 cut(s) | 266 |
| BmiI | GGNNCC | 1 cut(s) | 393 |
| BmrFI | CCNGG | 2 cut(s) | 41, 396 |
| BmsI | GCATC | 2 cut(s) | 21, 298 |
| BpiI | GAAGAC | 1 cut(s) | 354 |
| Bse3DI | GCAATG | 1 cut(s) | 273 |
| BseBI | CCWGG | 2 cut(s) | 41, 396 |
| BseMI | GCAATG | 1 cut(s) | 273 |
| BseRI | GAGGAG | 1 cut(s) | 181 |
| BseXI | GCAGC | 1 cut(s) | 152 |
| BsmI | GAATGC | 1 cut(s) | 307 |
| Bsp143I | GATC | 3 cut(s) | 224, 284, 391 |
| BspACI | CCGC | 1 cut(s) | 32 |
| BspLI | GGNNCC | 1 cut(s) | 393 |
| BspPI | GGATC | 3 cut(s) | 292, 386, 399 |
| BsrDI | GCAATG | 1 cut(s) | 273 |
| BssMI | GATC | 3 cut(s) | 224, 284, 391 |
| Bst2UI | CCWGG | 2 cut(s) | 41, 396 |
| Bst4CI | ACNGT | 3 cut(s) | 98, 340, 362 |
| BstDEI | CTNAG | 3 cut(s) | 144, 243, 456 |
| BstKTI | GATC | 3 cut(s) | 227, 287, 394 |
| BstMBI | GATC | 3 cut(s) | 224, 284, 391 |
| BstNI | CCWGG | 2 cut(s) | 41, 396 |
| BstSCI | CCNGG | 2 cut(s) | 39, 394 |
| BstV1I | GCAGC | 1 cut(s) | 152 |
| BstV2I | GAAGAC | 1 cut(s) | 354 |
| BstX2I | RGATCY | 1 cut(s) | 391 |
| BstYI | RGATCY | 1 cut(s) | 391 |
| BtsIMutI | CAGTG | 1 cut(s) | 103 |
| Cfr13I | GGNCC | 1 cut(s) | 266 |
| CviJI | RGCY | 1 cut(s) | 143 |
| CviKI_1 | RGCY | 1 cut(s) | 143 |
| DdeI | CTNAG | 3 cut(s) | 144, 243, 456 |
| DpnI | GATC | 3 cut(s) | 226, 286, 393 |
| DpnII | GATC | 3 cut(s) | 224, 284, 391 |
| DriI | GACNNNNNGTC | 1 cut(s) | 294 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 294 |
| Eco47I | GGWCC | 1 cut(s) | 266 |
| Eco57I | CTGAAG | 2 cut(s) | 213, 300 |
| EcoRII | CCWGG | 2 cut(s) | 39, 394 |
| EcoT22I | ATGCAT | 1 cut(s) | 313 |
| FaiI | YATR | 3 cut(s) | 234, 321, 401 |
| Fnu4HI | GCNGC | 2 cut(s) | 32, 141 |
| Fsp4HI | GCNGC | 2 cut(s) | 32, 141 |
| GluI | GCNGC | 2 cut(s) | 32, 141 |
| HincII | GTYRAC | 1 cut(s) | 238 |
| HindII | GTYRAC | 1 cut(s) | 238 |
| HinfI | GANTC | 1 cut(s) | 289 |
| HpaI | GTTAAC | 1 cut(s) | 238 |
| HphI | GGTGA | 1 cut(s) | 218 |
| Hpy166II | GTNNAC | 2 cut(s) | 238, 269 |
| Hpy188I | TCNGA | 2 cut(s) | 133, 300 |
| Hpy188III | TCNNGA | 2 cut(s) | 16, 108 |
| Hpy8I | GTNNAC | 2 cut(s) | 238, 269 |
| HpyAV | CCTTC | 2 cut(s) | 197, 275 |
| HpyCH4III | ACNGT | 3 cut(s) | 98, 340, 362 |
| HpyCH4V | TGCA | 5 cut(s) | 4, 103, 311, 344, 367 |
| HpyF3I | CTNAG | 3 cut(s) | 144, 243, 456 |
| KspAI | GTTAAC | 1 cut(s) | 238 |
| Kzo9I | GATC | 3 cut(s) | 224, 284, 391 |
| LmnI | GCTCC | 1 cut(s) | 9 |
| LpnPI | CCDG | 6 cut(s) | 26, 39, 53, 85, 381, 408 |
| Lsp1109I | GCAGC | 1 cut(s) | 152 |
| LweI | GCATC | 2 cut(s) | 21, 298 |
| MaeIII | GTNAC | 2 cut(s) | 151, 451 |
| MalI | GATC | 3 cut(s) | 226, 286, 393 |
| MboI | GATC | 3 cut(s) | 224, 284, 391 |
| MboII | GAAGA | 3 cut(s) | 206, 221, 359 |
| MflI | RGATCY | 1 cut(s) | 391 |
| MluCI | AATT | 4 cut(s) | 229, 332, 373, 420 |
| MlyI | GAGTC | 1 cut(s) | 283 |
| MnlI | CCTC | 3 cut(s) | 152, 159, 240 |
| Mph1103I | ATGCAT | 1 cut(s) | 313 |
| MseI | TTAA | 1 cut(s) | 237 |
| MspA1I | CMGCKG | 1 cut(s) | 34 |
| MspR9I | CCNGG | 2 cut(s) | 41, 396 |
| Mva1269I | GAATGC | 1 cut(s) | 307 |
| MvaI | CCWGG | 2 cut(s) | 41, 396 |
| NdeII | GATC | 3 cut(s) | 224, 284, 391 |
| NlaIV | GGNNCC | 1 cut(s) | 393 |
| NmuCI | GTSAC | 2 cut(s) | 151, 451 |
| NsiI | ATGCAT | 1 cut(s) | 313 |
| PctI | GAATGC | 1 cut(s) | 307 |
| PkrI | GCNGC | 2 cut(s) | 33, 142 |
| PleI | GAGTC | 1 cut(s) | 283 |
| PpsI | GAGTC | 1 cut(s) | 283 |
| Psp6I | CCWGG | 2 cut(s) | 39, 394 |
| PspGI | CCWGG | 2 cut(s) | 39, 394 |
| PspN4I | GGNNCC | 1 cut(s) | 393 |
| PspPI | GGNCC | 1 cut(s) | 266 |
| PsuI | RGATCY | 1 cut(s) | 391 |
| SaqAI | TTAA | 1 cut(s) | 237 |
| SatI | GCNGC | 2 cut(s) | 32, 141 |
| Sau3AI | GATC | 3 cut(s) | 224, 284, 391 |
| Sau96I | GGNCC | 1 cut(s) | 266 |
| SchI | GAGTC | 1 cut(s) | 283 |
| ScrFI | CCNGG | 2 cut(s) | 41, 396 |
| SetI | ASST | 5 cut(s) | 145, 208, 251, 400, 451 |
| SfaNI | GCATC | 2 cut(s) | 21, 298 |
| SinI | GGWCC | 1 cut(s) | 266 |
| Sse9I | AATT | 4 cut(s) | 229, 332, 373, 420 |
| SsiI | CCGC | 1 cut(s) | 32 |
| StyD4I | CCNGG | 2 cut(s) | 39, 394 |
| TaaI | ACNGT | 3 cut(s) | 98, 340, 362 |
| TaqI | TCGA | 2 cut(s) | 287, 292 |
| TasI | AATT | 4 cut(s) | 229, 332, 373, 420 |
| TauI | GCSGC | 1 cut(s) | 34 |
| Tru1I | TTAA | 1 cut(s) | 237 |
| Tru9I | TTAA | 1 cut(s) | 237 |
| TscAI | CASTG | 1 cut(s) | 103 |
| TseFI | GTSAC | 2 cut(s) | 151, 451 |
| TseI | GCWGC | 1 cut(s) | 140 |
| Tsp45I | GTSAC | 2 cut(s) | 151, 451 |
| TspRI | CASTG | 1 cut(s) | 103 |
| VpaK11BI | GGWCC | 1 cut(s) | 266 |
| XapI | RAATTY | 1 cut(s) | 373 |
| Zsp2I | ATGCAT | 1 cut(s) | 313 |