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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 292 |
| AccB1I | GGYRCC | 1 cut(s) | 292 |
| AclWI | GGATC | 3 cut(s) | 76, 119, 180 |
| AcuI | CTGAAG | 2 cut(s) | 50, 264 |
| AfaI | GTAC | 1 cut(s) | 294 |
| AgsI | TTSAA | 2 cut(s) | 161, 389 |
| Alw21I | GWGCWC | 1 cut(s) | 407 |
| AlwI | GGATC | 3 cut(s) | 76, 119, 180 |
| ApeKI | GCWGC | 1 cut(s) | 53 |
| Asp718I | GGTACC | 1 cut(s) | 292 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 273, 306 |
| BanI | GGYRCC | 1 cut(s) | 292 |
| Bbv12I | GWGCWC | 1 cut(s) | 407 |
| BbvI | GCAGC | 1 cut(s) | 40 |
| BccI | CCATC | 2 cut(s) | 136, 418 |
| BciVI | GTATCC | 1 cut(s) | 274 |
| BfaI | CTAG | 1 cut(s) | 149 |
| BfuI | GTATCC | 1 cut(s) | 274 |
| BisI | GCNGC | 1 cut(s) | 54 |
| BlsI | GCNGC | 1 cut(s) | 55 |
| BmiI | GGNNCC | 1 cut(s) | 294 |
| BmsI | GCATC | 3 cut(s) | 118, 142, 257 |
| Bse1I | ACTGG | 1 cut(s) | 334 |
| Bse3DI | GCAATG | 1 cut(s) | 117 |
| BseGI | GGATG | 1 cut(s) | 109 |
| BseMI | GCAATG | 1 cut(s) | 117 |
| BseMII | CTCAG | 2 cut(s) | 155, 363 |
| BseNI | ACTGG | 1 cut(s) | 334 |
| BseRI | GAGGAG | 2 cut(s) | 34, 325 |
| BseXI | GCAGC | 1 cut(s) | 40 |
| BshNI | GGYRCC | 1 cut(s) | 292 |
| BsiHKAI | GWGCWC | 1 cut(s) | 407 |
| BsmI | GAATGC | 1 cut(s) | 263 |
| Bsp1286I | GDGCHC | 1 cut(s) | 407 |
| Bsp143I | GATC | 4 cut(s) | 68, 73, 124, 172 |
| BspCNI | CTCAG | 2 cut(s) | 154, 364 |
| BspLI | GGNNCC | 1 cut(s) | 294 |
| BspPI | GGATC | 3 cut(s) | 76, 119, 180 |
| BspT107I | GGYRCC | 1 cut(s) | 292 |
| BsrDI | GCAATG | 1 cut(s) | 117 |
| BsrI | ACTGG | 1 cut(s) | 334 |
| BssMI | GATC | 4 cut(s) | 68, 73, 124, 172 |
| Bst6I | CTCTTC | 1 cut(s) | 26 |
| BstDEI | CTNAG | 4 cut(s) | 87, 141, 207, 372 |
| BstF5I | GGATG | 1 cut(s) | 109 |
| BstKTI | GATC | 4 cut(s) | 71, 76, 127, 175 |
| BstMBI | GATC | 4 cut(s) | 68, 73, 124, 172 |
| BstV1I | GCAGC | 1 cut(s) | 40 |
| BstX2I | RGATCY | 1 cut(s) | 68 |
| BstYI | RGATCY | 1 cut(s) | 68 |
| BsuI | GTATCC | 1 cut(s) | 274 |
| BtsCI | GGATG | 1 cut(s) | 109 |
| BtsI | GCAGTG | 1 cut(s) | 53 |
| BtsIMutI | CAGTG | 2 cut(s) | 53, 341 |
| Csp6I | GTAC | 1 cut(s) | 293 |
| CviAII | CATG | 1 cut(s) | 428 |
| CviQI | GTAC | 1 cut(s) | 293 |
| DdeI | CTNAG | 4 cut(s) | 87, 141, 207, 372 |
| DpnI | GATC | 4 cut(s) | 70, 75, 126, 174 |
| DpnII | GATC | 4 cut(s) | 68, 73, 124, 172 |
| Eam1104I | CTCTTC | 1 cut(s) | 26 |
| EarI | CTCTTC | 1 cut(s) | 26 |
| Eco57I | CTGAAG | 2 cut(s) | 50, 264 |
| EcoT22I | ATGCAT | 1 cut(s) | 157 |
| FaeI | CATG | 1 cut(s) | 431 |
| FaiI | YATR | 5 cut(s) | 165, 305, 401, 423, 429 |
| FatI | CATG | 1 cut(s) | 427 |
| Fnu4HI | GCNGC | 1 cut(s) | 54 |
| FokI | GGATG | 1 cut(s) | 96 |
| Fsp4HI | GCNGC | 1 cut(s) | 54 |
| FspBI | CTAG | 1 cut(s) | 149 |
| GluI | GCNGC | 1 cut(s) | 54 |
| Hin1II | CATG | 1 cut(s) | 431 |
| HincII | GTYRAC | 1 cut(s) | 82 |
| HindII | GTYRAC | 1 cut(s) | 82 |
| HinfI | GANTC | 3 cut(s) | 145, 188, 389 |
| HpaI | GTTAAC | 1 cut(s) | 82 |
| Hpy166II | GTNNAC | 1 cut(s) | 82 |
| Hpy188I | TCNGA | 4 cut(s) | 73, 144, 235, 244 |
| Hpy188III | TCNNGA | 1 cut(s) | 149 |
| Hpy8I | GTNNAC | 1 cut(s) | 82 |
| HpyCH4V | TGCA | 2 cut(s) | 109, 155 |
| HpyF3I | CTNAG | 4 cut(s) | 87, 141, 207, 372 |
| Hsp92II | CATG | 1 cut(s) | 431 |
| KpnI | GGTACC | 1 cut(s) | 296 |
| KspAI | GTTAAC | 1 cut(s) | 82 |
| Kzo9I | GATC | 4 cut(s) | 68, 73, 124, 172 |
| LpnPI | CCDG | 2 cut(s) | 210, 347 |
| Lsp1109I | GCAGC | 1 cut(s) | 40 |
| LweI | GCATC | 3 cut(s) | 118, 142, 257 |
| MaeI | CTAG | 1 cut(s) | 149 |
| MalI | GATC | 4 cut(s) | 70, 75, 126, 174 |
| MboI | GATC | 4 cut(s) | 68, 73, 124, 172 |
| MboII | GAAGA | 4 cut(s) | 17, 43, 192, 393 |
| MflI | RGATCY | 1 cut(s) | 68 |
| MhlI | GDGCHC | 1 cut(s) | 407 |
| MluCI | AATT | 3 cut(s) | 323, 357, 384 |
| Mph1103I | ATGCAT | 1 cut(s) | 157 |
| MseI | TTAA | 2 cut(s) | 81, 201 |
| Mva1269I | GAATGC | 1 cut(s) | 263 |
| NdeII | GATC | 4 cut(s) | 68, 73, 124, 172 |
| NlaIII | CATG | 1 cut(s) | 431 |
| NlaIV | GGNNCC | 1 cut(s) | 294 |
| NsiI | ATGCAT | 1 cut(s) | 157 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 337 |
| PctI | GAATGC | 1 cut(s) | 263 |
| PfeI | GAWTC | 3 cut(s) | 145, 188, 389 |
| PkrI | GCNGC | 1 cut(s) | 55 |
| PspN4I | GGNNCC | 1 cut(s) | 294 |
| PsuI | RGATCY | 1 cut(s) | 68 |
| RsaI | GTAC | 1 cut(s) | 294 |
| RsaNI | GTAC | 1 cut(s) | 293 |
| SaqAI | TTAA | 2 cut(s) | 81, 201 |
| SatI | GCNGC | 1 cut(s) | 54 |
| Sau3AI | GATC | 4 cut(s) | 68, 73, 124, 172 |
| SduI | GDGCHC | 1 cut(s) | 407 |
| SetI | ASST | 4 cut(s) | 95, 142, 215, 291 |
| SfaNI | GCATC | 3 cut(s) | 118, 142, 257 |
| SgeI | CNNG | 9 cut(s) | 89, 161, 188, 209, 264, 274, 346, 353, 389 |
| Sse9I | AATT | 3 cut(s) | 323, 357, 384 |
| SspMI | CTAG | 1 cut(s) | 149 |
| TasI | AATT | 3 cut(s) | 323, 357, 384 |
| TfiI | GAWTC | 3 cut(s) | 145, 188, 389 |
| Tru1I | TTAA | 2 cut(s) | 81, 201 |
| Tru9I | TTAA | 2 cut(s) | 81, 201 |
| TscAI | CASTG | 2 cut(s) | 53, 341 |
| TseI | GCWGC | 1 cut(s) | 53 |
| TspDTI | ATGAA | 3 cut(s) | 218, 332, 416 |
| TspRI | CASTG | 2 cut(s) | 53, 341 |
| XbaI | TCTAGA | 1 cut(s) | 148 |
| XspI | CTAG | 1 cut(s) | 149 |
| Zsp2I | ATGCAT | 1 cut(s) | 157 |