Rorug07G0248700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000782
Physical Location & Seq
Forward (+)
22993480 .. 22993980
501 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0248700.1

Sequence Viewer

Length: 501 bp
ATGGACCTTCTCTCTAATGCTTTTATTGCTTCTTTCTCCAAAGCCTCAAAGATTTTTTGGCGTATAACTTGTTGTAACCTCCTGTGGTGCACTTGGAATGAAAGGAATAAACATAAATTTGATGGGGGGCAGTTCTCATTTGCATGTTTCAAGCACTTCTTCGTAGGCTTTCTAAAGGATTCAGCGGGGTTGGCTTTTTCTCCCTCAGTGTATCCCTCCTCCTTGCCAATGTTTACTTTTCTACAGTTGTCACAATTGAGAGTTCATGCCCCACGCTTCATTTGCGTGGAATGGTATCCGCCACTGCATCATCCTTGCCTAAAGATTAATATAGATGGTTCCTTCAGAAGCCCGGTGATTGCGGGGTTTGGTGGGATTGTACGTGATCATGACGGTGGGTTTGTGCTTGCCTTTGCTTCTAAGGTCAGTGTTTGCAGCGCTTTGGATGCGGAAATATTAGCTTTTCTTGAAGCAGTTCAAGTTGCAAGGCTTCGGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

166

Amino Acids

18.64

Weight (kDa)

8.74

Isoelectric Point (pI)

43.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 110 - 163 5.1e-06 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 185, 299, 362, 449
AcsI RAATTY 1 cut(s) 116
AcuI CTGAAG 1 cut(s) 328
AfaI GTAC 1 cut(s) 381
AfeI AGCGCT 1 cut(s) 439
AgsI TTSAA 3 cut(s) 151, 470, 479
AluBI AGCT 1 cut(s) 461
AluI AGCT 1 cut(s) 461
Alw21I GWGCWC 1 cut(s) 92
Alw44I GTGCAC 1 cut(s) 88
Aor51HI AGCGCT 1 cut(s) 439
ApaLI GTGCAC 1 cut(s) 88
ApeKI GCWGC 1 cut(s) 435
ApoI RAATTY 1 cut(s) 116
ArsI GACNNNNNNTTYG 2 cut(s) 383, 415
AseI ATTAAT 1 cut(s) 327
Asp700I GAANNNNTTC 1 cut(s) 474
AspLEI GCGC 1 cut(s) 440
AspS9I GGNCC 1 cut(s) 4
AsuC2I CCSGG 1 cut(s) 353
AsuHPI GGTGA 1 cut(s) 367
AvaII GGWCC 1 cut(s) 4
BaeGI GKGCMC 1 cut(s) 92
Bbv12I GWGCWC 1 cut(s) 92
BbvI GCAGC 1 cut(s) 447
BccI CCATC 2 cut(s) 116, 329
BciVI GTATCC 2 cut(s) 222, 306
BclI TGATCA 1 cut(s) 385
BcnI CCSGG 1 cut(s) 353
BfmI CTRYAG 1 cut(s) 242
BfoI RGCGCY 1 cut(s) 441
BfuI GTATCC 2 cut(s) 222, 306
BisI GCNGC 1 cut(s) 436
BlsI GCNGC 1 cut(s) 437
Bme1390I CCNGG 1 cut(s) 353
Bme18I GGWCC 1 cut(s) 4
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 1 cut(s) 340
BmrFI CCNGG 1 cut(s) 353
BmsI GCATC 2 cut(s) 316, 436
BpuMI CCSGG 1 cut(s) 353
BsaAI YACGTR 1 cut(s) 383
BseGI GGATG 2 cut(s) 310, 451
BseMII CTCAG 1 cut(s) 219
BseRI GAGGAG 1 cut(s) 208
BseSI GKGCMC 1 cut(s) 92
BseXI GCAGC 1 cut(s) 447
BsiHKAI GWGCWC 1 cut(s) 92
BsiSI CCGG 1 cut(s) 353
Bsp1286I GDGCHC 1 cut(s) 92
Bsp143I GATC 1 cut(s) 385
BspACI CCGC 4 cut(s) 185, 299, 362, 449
BspCNI CTCAG 1 cut(s) 218
BspHI TCATGA 1 cut(s) 388
BspLI GGNNCC 1 cut(s) 340
BssMI GATC 1 cut(s) 385
Bst4CI ACNGT 2 cut(s) 246, 395
BstBAI YACGTR 1 cut(s) 383
BstC8I GCNNGC 1 cut(s) 408
BstDEI CTNAG 2 cut(s) 205, 420
BstF5I GGATG 2 cut(s) 310, 451
BstH2I RGCGCY 1 cut(s) 441
BstHHI GCGC 1 cut(s) 440
BstKTI GATC 1 cut(s) 388
BstMBI GATC 1 cut(s) 385
BstMWI GCNNNNNNNGC 4 cut(s) 26, 191, 282, 446
BstNSI RCATGY 1 cut(s) 147
BstSCI CCNGG 1 cut(s) 351
BstSFI CTRYAG 1 cut(s) 242
BstSLI GKGCMC 1 cut(s) 92
BstV1I GCAGC 1 cut(s) 447
BsuI GTATCC 2 cut(s) 222, 306
BtsCI GGATG 2 cut(s) 310, 451
BtsI GCAGTG 1 cut(s) 302
BtsIMutI CAGTG 3 cut(s) 213, 302, 433
Cac8I GCNNGC 1 cut(s) 408
CciI TCATGA 1 cut(s) 388
CfoI GCGC 1 cut(s) 440
Cfr13I GGNCC 1 cut(s) 4
Csp6I GTAC 1 cut(s) 380
CviAII CATG 3 cut(s) 144, 266, 389
CviJI RGCY 6 cut(s) 44, 168, 194, 351, 461, 490
CviKI_1 RGCY 6 cut(s) 44, 168, 194, 351, 461, 490
CviQI GTAC 1 cut(s) 380
DdeI CTNAG 2 cut(s) 205, 420
DpnI GATC 1 cut(s) 387
DpnII GATC 1 cut(s) 385
EciI GGCGGA 1 cut(s) 288
Eco47I GGWCC 1 cut(s) 4
Eco47III AGCGCT 1 cut(s) 439
Eco57I CTGAAG 1 cut(s) 328
FaeI CATG 3 cut(s) 147, 269, 392
FaiI YATR 6 cut(s) 65, 114, 145, 267, 332, 390
FalI AAGNNNNNCTT 2 cut(s) 143, 175
FatI CATG 3 cut(s) 143, 265, 388
FauI CCCGC 2 cut(s) 178, 355
FbaI TGATCA 1 cut(s) 385
Fnu4HI GCNGC 1 cut(s) 436
FokI GGATG 2 cut(s) 297, 458
Fsp4HI GCNGC 1 cut(s) 436
GlaI GCGC 1 cut(s) 439
GluI GCNGC 1 cut(s) 436
HaeII RGCGCY 1 cut(s) 441
HapII CCGG 1 cut(s) 353
HhaI GCGC 1 cut(s) 440
Hin1II CATG 3 cut(s) 147, 269, 392
Hin6I GCGC 1 cut(s) 438
HinP1I GCGC 1 cut(s) 438
HinfI GANTC 1 cut(s) 179
HpaII CCGG 1 cut(s) 353
HphI GGTGA 1 cut(s) 367
Hpy166II GTNNAC 2 cut(s) 90, 234
Hpy188I TCNGA 1 cut(s) 347
Hpy188III TCNNGA 3 cut(s) 389, 467, 494
Hpy8I GTNNAC 2 cut(s) 90, 234
HpyAV CCTTC 2 cut(s) 17, 352
HpyCH4III ACNGT 2 cut(s) 246, 395
HpyCH4IV ACGT 1 cut(s) 382
HpyCH4V TGCA 5 cut(s) 90, 143, 307, 435, 485
HpyF10VI GCNNNNNNNGC 4 cut(s) 26, 191, 282, 446
HpyF3I CTNAG 2 cut(s) 205, 420
HpySE526I ACGT 1 cut(s) 382
Hsp92II CATG 3 cut(s) 147, 269, 392
HspAI GCGC 1 cut(s) 438
Ksp22I TGATCA 1 cut(s) 385
Kzo9I GATC 1 cut(s) 385
LpnPI CCDG 2 cut(s) 95, 366
Lsp1109I GCAGC 1 cut(s) 447
LweI GCATC 2 cut(s) 316, 436
MaeII ACGT 1 cut(s) 382
MaeIII GTNAC 2 cut(s) 74, 249
MalI GATC 1 cut(s) 387
MboI GATC 1 cut(s) 385
MboII GAAGA 1 cut(s) 151
MfeI CAATTG 1 cut(s) 254
MhlI GDGCHC 1 cut(s) 92
MluCI AATT 2 cut(s) 116, 254
MnlI CCTC 5 cut(s) 55, 89, 214, 226, 229
MroXI GAANNNNTTC 1 cut(s) 474
MseI TTAA 1 cut(s) 327
MslI CAYNNNNRTG 3 cut(s) 142, 284, 393
MspA1I CMGCKG 1 cut(s) 185
MspI CCGG 1 cut(s) 353
MspR9I CCNGG 1 cut(s) 353
MunI CAATTG 1 cut(s) 254
MwoI GCNNNNNNNGC 4 cut(s) 26, 191, 282, 446
NciI CCSGG 1 cut(s) 353
NdeII GATC 1 cut(s) 385
NlaIII CATG 3 cut(s) 147, 269, 392
NlaIV GGNNCC 1 cut(s) 340
NmuCI GTSAC 1 cut(s) 249
NspI RCATGY 1 cut(s) 147
PagI TCATGA 1 cut(s) 388
PdmI GAANNNNTTC 1 cut(s) 474
PfeI GAWTC 1 cut(s) 179
PkrI GCNGC 1 cut(s) 437
Ppu21I YACGTR 1 cut(s) 383
PshBI ATTAAT 1 cut(s) 327
PspN4I GGNNCC 1 cut(s) 340
PspPI GGNCC 1 cut(s) 4
RsaI GTAC 1 cut(s) 381
RsaNI GTAC 1 cut(s) 380
RseI CAYNNNNRTG 3 cut(s) 142, 284, 393
SaqAI TTAA 1 cut(s) 327
SatI GCNGC 1 cut(s) 436
Sau3AI GATC 1 cut(s) 385
Sau96I GGNCC 1 cut(s) 4
ScrFI CCNGG 1 cut(s) 353
SduI GDGCHC 1 cut(s) 92
SetI ASST 5 cut(s) 9, 81, 385, 426, 463
SfaNI GCATC 2 cut(s) 316, 436
SfcI CTRYAG 1 cut(s) 242
SinI GGWCC 1 cut(s) 4
SmiMI CAYNNNNRTG 3 cut(s) 142, 284, 393
Sse9I AATT 2 cut(s) 116, 254
SsiI CCGC 4 cut(s) 185, 299, 362, 449
SspI AATATT 1 cut(s) 456
StyD4I CCNGG 1 cut(s) 351
TaaI ACNGT 2 cut(s) 246, 395
TaiI ACGT 1 cut(s) 385
TasI AATT 2 cut(s) 116, 254
TfiI GAWTC 1 cut(s) 179
Tru1I TTAA 1 cut(s) 327
Tru9I TTAA 1 cut(s) 327
TscAI CASTG 3 cut(s) 213, 309, 433
TseFI GTSAC 1 cut(s) 249
TseI GCWGC 1 cut(s) 435
Tsp45I GTSAC 1 cut(s) 249
TspDTI ATGAA 3 cut(s) 114, 254, 268
TspRI CASTG 3 cut(s) 213, 309, 433
VneI GTGCAC 1 cut(s) 88
VpaK11BI GGWCC 1 cut(s) 4
VspI ATTAAT 1 cut(s) 327
XapI RAATTY 1 cut(s) 116
XceI RCATGY 1 cut(s) 147
XmnI GAANNNNTTC 1 cut(s) 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.