Protein of unknown function (DUF 659)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 194 |
| AfaI | GTAC | 2 cut(s) | 90, 284 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 285 |
| AgsI | TTSAA | 3 cut(s) | 28, 155, 185 |
| AluBI | AGCT | 1 cut(s) | 150 |
| AluI | AGCT | 1 cut(s) | 150 |
| AoxI | GGCC | 1 cut(s) | 208 |
| ApoI | RAATTY | 1 cut(s) | 194 |
| AsuHPI | GGTGA | 1 cut(s) | 278 |
| BccI | CCATC | 1 cut(s) | 263 |
| BfaI | CTAG | 1 cut(s) | 257 |
| BmiI | GGNNCC | 1 cut(s) | 234 |
| BmsI | GCATC | 3 cut(s) | 40, 214, 279 |
| BsaAI | YACGTR | 1 cut(s) | 282 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 285 |
| BseGI | GGATG | 2 cut(s) | 229, 294 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 285 |
| BseRI | GAGGAG | 1 cut(s) | 111 |
| BshFI | GGCC | 1 cut(s) | 210 |
| BsiWI | CGTACG | 1 cut(s) | 282 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 285 |
| BsnI | GGCC | 1 cut(s) | 210 |
| Bsp1407I | TGTACA | 1 cut(s) | 88 |
| BspANI | GGCC | 1 cut(s) | 210 |
| BspLI | GGNNCC | 1 cut(s) | 234 |
| BsrGI | TGTACA | 1 cut(s) | 88 |
| BstAUI | TGTACA | 1 cut(s) | 88 |
| BstBAI | YACGTR | 1 cut(s) | 282 |
| BstF5I | GGATG | 2 cut(s) | 229, 294 |
| BsuRI | GGCC | 1 cut(s) | 210 |
| BtsCI | GGATG | 2 cut(s) | 229, 294 |
| Csp6I | GTAC | 2 cut(s) | 89, 283 |
| CviJI | RGCY | 2 cut(s) | 150, 210 |
| CviKI_1 | RGCY | 2 cut(s) | 150, 210 |
| CviQI | GTAC | 2 cut(s) | 89, 283 |
| DraI | TTTAAA | 1 cut(s) | 192 |
| FaiI | YATR | 5 cut(s) | 12, 37, 144, 230, 298 |
| FokI | GGATG | 1 cut(s) | 236 |
| FspBI | CTAG | 1 cut(s) | 257 |
| HaeIII | GGCC | 1 cut(s) | 210 |
| HphI | GGTGA | 1 cut(s) | 278 |
| Hpy166II | GTNNAC | 1 cut(s) | 276 |
| Hpy8I | GTNNAC | 1 cut(s) | 276 |
| HpyAV | CCTTC | 1 cut(s) | 13 |
| HpyCH4IV | ACGT | 1 cut(s) | 281 |
| HpySE526I | ACGT | 1 cut(s) | 281 |
| LweI | GCATC | 3 cut(s) | 40, 214, 279 |
| MaeI | CTAG | 1 cut(s) | 257 |
| MaeII | ACGT | 1 cut(s) | 281 |
| MaeIII | GTNAC | 2 cut(s) | 40, 169 |
| MluCI | AATT | 5 cut(s) | 23, 78, 98, 194, 237 |
| MnlI | CCTC | 4 cut(s) | 64, 125, 132, 208 |
| MseI | TTAA | 5 cut(s) | 65, 72, 84, 101, 191 |
| MslI | CAYNNNNRTG | 1 cut(s) | 36 |
| NlaIV | GGNNCC | 1 cut(s) | 234 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 250 |
| Pfl23II | CGTACG | 1 cut(s) | 282 |
| Ppu21I | YACGTR | 1 cut(s) | 282 |
| PspLI | CGTACG | 1 cut(s) | 282 |
| PspN4I | GGNNCC | 1 cut(s) | 234 |
| RsaI | GTAC | 2 cut(s) | 90, 284 |
| RsaNI | GTAC | 2 cut(s) | 89, 283 |
| RseI | CAYNNNNRTG | 1 cut(s) | 36 |
| SaqAI | TTAA | 5 cut(s) | 65, 72, 84, 101, 191 |
| SetI | ASST | 5 cut(s) | 50, 107, 152, 219, 284 |
| SfaNI | GCATC | 3 cut(s) | 40, 214, 279 |
| SgeI | CNNG | 5 cut(s) | 57, 224, 257, 269, 292 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 36 |
| Sse9I | AATT | 5 cut(s) | 23, 78, 98, 194, 237 |
| SspMI | CTAG | 1 cut(s) | 257 |
| TaiI | ACGT | 1 cut(s) | 284 |
| TaqI | TCGA | 1 cut(s) | 244 |
| TasI | AATT | 5 cut(s) | 23, 78, 98, 194, 237 |
| TatI | WGTACW | 1 cut(s) | 88 |
| Tru1I | TTAA | 5 cut(s) | 65, 72, 84, 101, 191 |
| Tru9I | TTAA | 5 cut(s) | 65, 72, 84, 101, 191 |
| TspDTI | ATGAA | 1 cut(s) | 111 |
| XapI | RAATTY | 1 cut(s) | 194 |
| XspI | CTAG | 1 cut(s) | 257 |