Rw5G024280

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000698
Physical Location & Seq
Reverse (-)
34515565 .. 34518042
2478 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G024280.1

Sequence Viewer

Length: 651 bp
ATGGCATCGTCCGGAGGATCTAATGCTACTATTACTACGAATCAAGTTGATAGATCATTGAGGCGTGGGTCAACCAATGCTGGATGGGACTTTGTGGTGTTGGCGGATCCACACAACTTAGATAAATTAAAGTTTGGATGGTGGTCCAATTATGGAAATAGAACACCCAAATTGCAAAAAATGGCTAGAAGAATACTCTCTTCAACTACAAGTTCATCCGGGTGCGAGAGAAATTGGAGTACTTTTGAGGGAATCCATTCAAAGAAAAGGAATAGACTAGATGTTTCAAGGTTTAACAATCTACTCTATGTCCAATTCAATGCCAAGATCATCAACAAAAAAAGAAGGGCTCAAGAGTTGGGTGTAGATGTGCTACTTGGTAATGAAGCTAGTAGGGCTCAATGGTGGATTGTTGATGGTGGTAATGAAGAAGATGACTTAGATATTACTAGTGAAATGGAGGGAGAGGCTTCAGGAGTGGATAGTGGGCCTAGGAGAAGCTCTAGAAATGTAGAGGTAAGAGAACTTCATGATGAAGATTTTGTATCGGATGAGGACACGGAAGAGGAGGGAGAAGAAGAGGATGTTGAGTTTGCGTCCAATACCGAAGGAGTCTTGGATGGATATGGAGAAGAAGAGTTAGAGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT

Protein Analysis

216

Amino Acids

24.3

Weight (kDa)

4.65

Isoelectric Point (pI)

51.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimer_Tnp_hAT PF05699 47 - 107 2.3e-12 hAT family C-terminal dimerisation region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000698)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G43260 AT5G31412
fragaria_vesca FvH4_2g24751 FvH4_3g29751 FvH4_3g29752 FvH4_3g32705 FvH4_4g20841 FvH4_5g39742 FvH4_6g06672 FvH4_6g28272 FvH4_6g28273 FvH4_7g00215
malus_domestica MD07G1147000.v1.1
prunus_persica Prupe.4G255800_v2.0.a1
pyrus_communis pycom09g15950 pycom10g13140
rosa_chinensis RchiOBHm_Chr1g0314441 RchiOBHm_Chr1g0314451 RchiOBHm_Chr4g0430441 RchiOBHm_Chr4g0430831 RchiOBHm_Chr5g0003001 RchiOBHm_Chr5g0033441 RchiOBHm_Chr6g0251571 RchiOBHm_Chr6g0288501
rosa_multiflora Rmu_co8007098.1_g000001 Rmu_co8211970.1_g000002 Rmu_sc0000146.1_g000031 Rmu_sc0000555.1_g000022 Rmu_sc0000968.1_g000005 Rmu_sc0001017.1_g000028 Rmu_sc0001208.1_g000044 Rmu_sc0001526.1_g000038 Rmu_sc0002226.1_g000026 Rmu_sc0002284.1_g000006 Rmu_sc0002776.1_g000004 Rmu_sc0003369.1_g000011 Rmu_sc0003632.1_g000005 Rmu_sc0004180.1_g000007 Rmu_sc0004180.1_g000008 Rmu_sc0005014.1_g000005 Rmu_sc0005023.1_g000011 Rmu_sc0007296.1_g000009 Rmu_sc0011153.1_g000002 Rmu_sc0011153.1_g000003 Rmu_sc0018472.1_g000003 Rmu_sc0020646.1_g000001 Rmu_sc0022773.1_g000001 Rmu_sc0022773.1_g000002 Rmu_sc0023555.1_g000001 Rmu_sc0023788.1_g000001 Rmu_ssc0000409.1_g000026 Rmu_ssc0000421.1_g000039
rosa_roxburghii Rroxscaffold_2G00091530 Rroxscaffold_5G00355490 Rroxscaffold_6G00405690 Rroxscaffold_6G00412290 Rroxscaffold_7G00195870
rosa_rugosa Rorug05G0244000
rosa_samantha Rh3DG075100
rosa_wichuraiana Rw0G014340 Rw1G023020 Rw2G020120 Rw2G033790 Rw2G035670 Rw3G022290 Rw3G023070 Rw3G023080 Rw4G003460 Rw4G017840 Rw4G032430 Rw5G024280 Rw5G044440 Rw6G027410 Rw7G020330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 11
AciI CCGC 1 cut(s) 104
AclWI GGATC 3 cut(s) 25, 101, 114
AcuI CTGAAG 1 cut(s) 456
AfaI GTAC 1 cut(s) 241
AgsI TTSAA 4 cut(s) 204, 261, 288, 319
AhlI ACTAGT 1 cut(s) 449
AluBI AGCT 2 cut(s) 389, 501
AluI AGCT 2 cut(s) 389, 501
AlwI GGATC 3 cut(s) 25, 101, 114
Aor13HI TCCGGA 1 cut(s) 11
AoxI GGCC 1 cut(s) 488
Asp700I GAANNNNTTC 1 cut(s) 256
AspA2I CCTAGG 1 cut(s) 491
AspS9I GGNCC 2 cut(s) 144, 488
AsuC2I CCSGG 1 cut(s) 220
AvaII GGWCC 1 cut(s) 144
AvrII CCTAGG 1 cut(s) 491
BamHI GGATCC 1 cut(s) 106
BanII GRGCYC 2 cut(s) 352, 400
BarI GAAGNNNNNNTAC 2 cut(s) 510, 542
BccI CCATC 4 cut(s) 78, 132, 410, 614
BcnI CCSGG 1 cut(s) 220
BcuI ACTAGT 1 cut(s) 449
BfaI CTAG 6 cut(s) 186, 278, 390, 450, 492, 504
BlnI CCTAGG 1 cut(s) 491
BmcAI AGTACT 1 cut(s) 241
Bme1390I CCNGG 1 cut(s) 220
Bme18I GGWCC 1 cut(s) 144
BmgT120I GGNCC 2 cut(s) 144, 488
BmiI GGNNCC 1 cut(s) 108
BmrFI CCNGG 1 cut(s) 220
BmsI GCATC 1 cut(s) 14
BpuEI CTTGAG 1 cut(s) 336
BpuMI CCSGG 1 cut(s) 220
BsaJI CCNNGG 1 cut(s) 491
BsaWI WCCGGW 1 cut(s) 11
BseAI TCCGGA 1 cut(s) 11
BseDI CCNNGG 1 cut(s) 491
BseGI GGATG 6 cut(s) 89, 143, 215, 556, 589, 625
BseRI GAGGAG 1 cut(s) 581
BshFI GGCC 1 cut(s) 490
BsiSI CCGG 2 cut(s) 12, 219
BslFI GGGAC 1 cut(s) 101
BsmFI GGGAC 1 cut(s) 101
BsnI GGCC 1 cut(s) 490
Bsp1286I GDGCHC 2 cut(s) 352, 400
Bsp13I TCCGGA 1 cut(s) 11
Bsp143I GATC 4 cut(s) 17, 53, 106, 327
BspACI CCGC 1 cut(s) 104
BspANI GGCC 1 cut(s) 490
BspEI TCCGGA 1 cut(s) 11
BspHI TCATGA 1 cut(s) 529
BspLI GGNNCC 1 cut(s) 108
BspPI GGATC 3 cut(s) 25, 101, 114
BssECI CCNNGG 1 cut(s) 491
BssMI GATC 4 cut(s) 17, 53, 106, 327
BssT1I CCWWGG 1 cut(s) 491
Bst6I CTCTTC 4 cut(s) 205, 558, 573, 630
BstDEI CTNAG 2 cut(s) 118, 439
BstF5I GGATG 6 cut(s) 89, 143, 215, 556, 589, 625
BstKTI GATC 4 cut(s) 20, 56, 109, 330
BstMBI GATC 4 cut(s) 17, 53, 106, 327
BstMWI GCNNNNNNNGC 1 cut(s) 395
BstSCI CCNGG 1 cut(s) 218
BstX2I RGATCY 2 cut(s) 17, 106
BstYI RGATCY 2 cut(s) 17, 106
BsuRI GGCC 1 cut(s) 490
BtsCI GGATG 6 cut(s) 89, 143, 215, 556, 589, 625
CciI TCATGA 1 cut(s) 529
Cfr13I GGNCC 2 cut(s) 144, 488
CseI GACGC 1 cut(s) 585
Csp6I GTAC 1 cut(s) 240
CviAII CATG 1 cut(s) 530
CviJI RGCY 7 cut(s) 185, 350, 389, 398, 470, 490, 501
CviKI_1 RGCY 7 cut(s) 185, 350, 389, 398, 470, 490, 501
CviQI GTAC 1 cut(s) 240
DdeI CTNAG 2 cut(s) 118, 439
DpnI GATC 4 cut(s) 19, 55, 108, 329
DpnII GATC 4 cut(s) 17, 53, 106, 327
Eam1104I CTCTTC 4 cut(s) 205, 558, 573, 630
EarI CTCTTC 4 cut(s) 205, 558, 573, 630
EciI GGCGGA 1 cut(s) 119
Eco130I CCWWGG 1 cut(s) 491
Eco24I GRGCYC 2 cut(s) 352, 400
Eco47I GGWCC 1 cut(s) 144
Eco57I CTGAAG 1 cut(s) 456
EcoT14I CCWWGG 1 cut(s) 491
EcoT38I GRGCYC 2 cut(s) 352, 400
ErhI CCWWGG 1 cut(s) 491
FaeI CATG 1 cut(s) 533
FaiI YATR 4 cut(s) 153, 309, 531, 627
FaqI GGGAC 1 cut(s) 101
FatI CATG 1 cut(s) 529
FokI GGATG 6 cut(s) 96, 150, 202, 563, 596, 632
FriOI GRGCYC 2 cut(s) 352, 400
FspBI CTAG 6 cut(s) 186, 278, 390, 450, 492, 504
HaeIII GGCC 1 cut(s) 490
HapII CCGG 2 cut(s) 12, 219
HgaI GACGC 1 cut(s) 585
Hin1II CATG 1 cut(s) 533
HincII GTYRAC 1 cut(s) 72
HindII GTYRAC 1 cut(s) 72
HinfI GANTC 3 cut(s) 40, 252, 612
HpaII CCGG 2 cut(s) 12, 219
Hpy166II GTNNAC 1 cut(s) 72
Hpy188I TCNGA 1 cut(s) 550
Hpy188III TCNNGA 5 cut(s) 12, 353, 474, 504, 530
Hpy8I GTNNAC 1 cut(s) 72
HpyAV CCTTC 2 cut(s) 339, 602
HpyCH4V TGCA 1 cut(s) 175
HpyF10VI GCNNNNNNNGC 1 cut(s) 395
HpyF3I CTNAG 2 cut(s) 118, 439
Hsp92II CATG 1 cut(s) 533
Kpn2I TCCGGA 1 cut(s) 11
Kzo9I GATC 4 cut(s) 17, 53, 106, 327
LpnPI CCDG 4 cut(s) 25, 66, 232, 459
LweI GCATC 1 cut(s) 14
MaeI CTAG 6 cut(s) 186, 278, 390, 450, 492, 504
MalI GATC 4 cut(s) 19, 55, 108, 329
MboI GATC 4 cut(s) 17, 53, 106, 327
MflI RGATCY 2 cut(s) 17, 106
MhlI GDGCHC 2 cut(s) 352, 400
MluCI AATT 5 cut(s) 125, 148, 170, 232, 314
MlyI GAGTC 1 cut(s) 621
MroI TCCGGA 1 cut(s) 11
MroXI GAANNNNTTC 1 cut(s) 256
MseI TTAA 2 cut(s) 128, 294
MslI CAYNNNNRTG 1 cut(s) 220
MspI CCGG 2 cut(s) 12, 219
MspR9I CCNGG 1 cut(s) 220
MwoI GCNNNNNNNGC 1 cut(s) 395
NciI CCSGG 1 cut(s) 220
NdeII GATC 4 cut(s) 17, 53, 106, 327
NlaIII CATG 1 cut(s) 533
NlaIV GGNNCC 1 cut(s) 108
PagI TCATGA 1 cut(s) 529
PdmI GAANNNNTTC 1 cut(s) 256
PfeI GAWTC 2 cut(s) 40, 252
PleI GAGTC 1 cut(s) 620
PpsI GAGTC 1 cut(s) 620
PspN4I GGNNCC 1 cut(s) 108
PspPI GGNCC 2 cut(s) 144, 488
PsuI RGATCY 2 cut(s) 17, 106
RsaI GTAC 1 cut(s) 241
RsaNI GTAC 1 cut(s) 240
RseI CAYNNNNRTG 1 cut(s) 220
SaqAI TTAA 2 cut(s) 128, 294
Sau3AI GATC 4 cut(s) 17, 53, 106, 327
Sau96I GGNCC 2 cut(s) 144, 488
ScaI AGTACT 1 cut(s) 241
SchI GAGTC 1 cut(s) 621
ScrFI CCNGG 1 cut(s) 220
SduI GDGCHC 2 cut(s) 352, 400
SetI ASST 5 cut(s) 293, 391, 503, 519, 648
SfaNI GCATC 1 cut(s) 14
SinI GGWCC 1 cut(s) 144
SmiMI CAYNNNNRTG 1 cut(s) 220
SmlI CTYRAG 1 cut(s) 351
SmoI CTYRAG 1 cut(s) 351
SpeI ACTAGT 1 cut(s) 449
Sse9I AATT 5 cut(s) 125, 148, 170, 232, 314
SsiI CCGC 1 cut(s) 104
SspMI CTAG 6 cut(s) 186, 278, 390, 450, 492, 504
StyD4I CCNGG 1 cut(s) 218
StyI CCWWGG 1 cut(s) 491
TasI AATT 5 cut(s) 125, 148, 170, 232, 314
TatI WGTACW 1 cut(s) 239
TfiI GAWTC 2 cut(s) 40, 252
Tru1I TTAA 2 cut(s) 128, 294
Tru9I TTAA 2 cut(s) 128, 294
TspDTI ATGAA 5 cut(s) 204, 399, 441, 518, 549
TspGWI ACGGA 1 cut(s) 575
VpaK11BI GGWCC 1 cut(s) 144
XbaI TCTAGA 1 cut(s) 503
XmaJI CCTAGG 1 cut(s) 491
XmnI GAANNNNTTC 1 cut(s) 256
XspI CTAG 6 cut(s) 186, 278, 390, 450, 492, 504
ZrmI AGTACT 1 cut(s) 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.