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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1642 |
| AccII | CGCG | 1 cut(s) | 2222 |
| AciI | CCGC | 9 cut(s) | 50, 59, 271, 350, 914, 917, 1224, 1517, 2222 |
| AclWI | GGATC | 1 cut(s) | 1736 |
| AcoI | YGGCCR | 1 cut(s) | 1454 |
| AcsI | RAATTY | 4 cut(s) | 178, 652, 922, 1016 |
| AdeI | CACNNNGTG | 1 cut(s) | 473 |
| AfaI | GTAC | 1 cut(s) | 1855 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 714, 1290, 1376, 1452, 1642, 2222 |
| AjnI | CCWGG | 2 cut(s) | 1126, 2266 |
| AluBI | AGCT | 4 cut(s) | 543, 1170, 1706, 2003 |
| AluI | AGCT | 4 cut(s) | 543, 1170, 1706, 2003 |
| Alw21I | GWGCWC | 1 cut(s) | 1283 |
| Alw26I | GTCTC | 2 cut(s) | 1107, 1625 |
| AlwI | GGATC | 1 cut(s) | 1736 |
| Ama87I | CYCGRG | 1 cut(s) | 580 |
| AoxI | GGCC | 9 cut(s) | 326, 347, 362, 577, 819, 941, 1124, 1443, 1454 |
| ApeKI | GCWGC | 1 cut(s) | 212 |
| ApoI | RAATTY | 4 cut(s) | 178, 652, 922, 1016 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1069, 1101 |
| AseI | ATTAAT | 1 cut(s) | 441 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1560 |
| AspS9I | GGNCC | 6 cut(s) | 326, 362, 578, 819, 1221, 1758 |
| AsuC2I | CCSGG | 4 cut(s) | 581, 582, 817, 1834 |
| AsuHPI | GGTGA | 3 cut(s) | 1370, 1657, 2048 |
| AsuNHI | GCTAGC | 1 cut(s) | 2003 |
| AvaI | CYCGRG | 1 cut(s) | 580 |
| AvaII | GGWCC | 2 cut(s) | 1221, 1758 |
| BaeGI | GKGCMC | 1 cut(s) | 868 |
| BanII | GRGCYC | 1 cut(s) | 1966 |
| BbsI | GAAGAC | 3 cut(s) | 1419, 1563, 2076 |
| Bbv12I | GWGCWC | 1 cut(s) | 1283 |
| BbvI | GCAGC | 1 cut(s) | 224 |
| BccI | CCATC | 8 cut(s) | 33, 404, 1168, 1349, 1378, 1746, 2013, 2024 |
| BciT130I | CCWGG | 2 cut(s) | 1128, 2268 |
| BclI | TGATCA | 1 cut(s) | 1570 |
| BcnI | CCSGG | 4 cut(s) | 581, 582, 817, 1834 |
| BcoDI | GTCTC | 2 cut(s) | 1107, 1625 |
| BfaI | CTAG | 7 cut(s) | 492, 1137, 1800, 1892, 1916, 2004, 2118 |
| BisI | GCNGC | 4 cut(s) | 213, 350, 915, 1518 |
| BlsI | GCNGC | 4 cut(s) | 214, 351, 916, 1519 |
| BmcAI | AGTACT | 1 cut(s) | 1855 |
| Bme1390I | CCNGG | 6 cut(s) | 581, 582, 817, 1128, 1834, 2268 |
| Bme18I | GGWCC | 2 cut(s) | 1221, 1758 |
| BmeT110I | CYCGRG | 1 cut(s) | 580 |
| BmgT120I | GGNCC | 6 cut(s) | 326, 362, 578, 819, 1221, 1758 |
| BmiI | GGNNCC | 1 cut(s) | 820 |
| BmrFI | CCNGG | 6 cut(s) | 581, 582, 817, 1128, 1834, 2268 |
| BmsI | GCATC | 9 cut(s) | 52, 178, 389, 414, 436, 487, 700, 811, 1885 |
| BmtI | GCTAGC | 1 cut(s) | 2007 |
| BpiI | GAAGAC | 3 cut(s) | 1419, 1563, 2076 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 1383, 1415, 1948, 1980 |
| BpmI | CTGGAG | 1 cut(s) | 2250 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1248, 2105 |
| BpuEI | CTTGAG | 5 cut(s) | 644, 1385, 1649, 1950, 1998 |
| BpuMI | CCSGG | 4 cut(s) | 581, 582, 817, 1834 |
| BsaBI | GATNNNNATC | 1 cut(s) | 801 |
| BsaI | GGTCTC | 1 cut(s) | 1625 |
| BsaJI | CCNNGG | 3 cut(s) | 580, 816, 1486 |
| BsaWI | WCCGGW | 1 cut(s) | 1744 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 714, 1290, 1376, 1452, 1642, 2222 |
| Bse118I | RCCGGY | 1 cut(s) | 509 |
| Bse3DI | GCAATG | 1 cut(s) | 141 |
| Bse8I | GATNNNNATC | 1 cut(s) | 801 |
| BseBI | CCWGG | 2 cut(s) | 1128, 2268 |
| BseDI | CCNNGG | 3 cut(s) | 580, 816, 1486 |
| BseJI | GATNNNNATC | 1 cut(s) | 801 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 714, 1290, 1376, 1452, 1642, 2222 |
| BseMI | GCAATG | 1 cut(s) | 141 |
| BseSI | GKGCMC | 1 cut(s) | 868 |
| BseXI | GCAGC | 1 cut(s) | 224 |
| Bsh1236I | CGCG | 1 cut(s) | 2222 |
| BshFI | GGCC | 9 cut(s) | 328, 349, 364, 579, 821, 943, 1126, 1445, 1456 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1283 |
| BsiHKCI | CYCGRG | 1 cut(s) | 580 |
| BsiSI | CCGG | 5 cut(s) | 510, 581, 816, 1745, 1833 |
| BslFI | GGGAC | 1 cut(s) | 56 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 714, 1290, 1376, 1452, 1642, 2222 |
| BsmAI | GTCTC | 2 cut(s) | 1107, 1625 |
| BsmFI | GGGAC | 1 cut(s) | 56 |
| BsmI | GAATGC | 3 cut(s) | 513, 1046, 1427 |
| BsnI | GGCC | 9 cut(s) | 328, 349, 364, 579, 821, 943, 1126, 1445, 1456 |
| Bso31I | GGTCTC | 1 cut(s) | 1625 |
| BsoBI | CYCGRG | 1 cut(s) | 580 |
| Bsp1286I | GDGCHC | 3 cut(s) | 868, 1283, 1966 |
| Bsp143I | GATC | 5 cut(s) | 8, 1570, 1741, 2166, 2203 |
| BspACI | CCGC | 9 cut(s) | 50, 59, 271, 350, 914, 917, 1224, 1517, 2222 |
| BspANI | GGCC | 9 cut(s) | 328, 349, 364, 579, 821, 943, 1126, 1445, 1456 |
| BspFNI | CGCG | 1 cut(s) | 2222 |
| BspLI | GGNNCC | 1 cut(s) | 820 |
| BspOI | GCTAGC | 1 cut(s) | 2007 |
| BspPI | GGATC | 1 cut(s) | 1736 |
| BspQI | GCTCTTC | 1 cut(s) | 1690 |
| BspTNI | GGTCTC | 1 cut(s) | 1625 |
| BsrDI | GCAATG | 1 cut(s) | 141 |
| BsrFI | RCCGGY | 1 cut(s) | 509 |
| BssAI | RCCGGY | 1 cut(s) | 509 |
| BssECI | CCNNGG | 3 cut(s) | 580, 816, 1486 |
| BssMI | GATC | 5 cut(s) | 8, 1570, 1741, 2166, 2203 |
| Bst2UI | CCWGG | 2 cut(s) | 1128, 2268 |
| Bst4CI | ACNGT | 2 cut(s) | 1273, 2251 |
| Bst6I | CTCTTC | 4 cut(s) | 627, 669, 1178, 1690 |
| BstC8I | GCNNGC | 3 cut(s) | 511, 1228, 2005 |
| BstDEI | CTNAG | 6 cut(s) | 73, 611, 1239, 1248, 1707, 2105 |
| BstDSI | CCRYGG | 1 cut(s) | 1486 |
| BstEII | GGTNACC | 2 cut(s) | 584, 1004 |
| BstFNI | CGCG | 1 cut(s) | 2222 |
| BstKTI | GATC | 5 cut(s) | 11, 1573, 1744, 2169, 2206 |
| BstMAI | GTCTC | 2 cut(s) | 1107, 1625 |
| BstMBI | GATC | 5 cut(s) | 8, 1570, 1741, 2166, 2203 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 56, 197, 768, 2009 |
| BstNI | CCWGG | 2 cut(s) | 1128, 2268 |
| BstPI | GGTNACC | 2 cut(s) | 584, 1004 |
| BstSCI | CCNGG | 6 cut(s) | 579, 580, 815, 1126, 1832, 2266 |
| BstSLI | GKGCMC | 1 cut(s) | 868 |
| BstUI | CGCG | 1 cut(s) | 2222 |
| BstV1I | GCAGC | 1 cut(s) | 224 |
| BstV2I | GAAGAC | 3 cut(s) | 1419, 1563, 2076 |
| BsuRI | GGCC | 9 cut(s) | 328, 349, 364, 579, 821, 943, 1126, 1445, 1456 |
| BtgI | CCRYGG | 1 cut(s) | 1486 |
| Cac8I | GCNNGC | 3 cut(s) | 511, 1228, 2005 |
| Cfr10I | RCCGGY | 1 cut(s) | 509 |
| Cfr13I | GGNCC | 6 cut(s) | 326, 362, 578, 819, 1221, 1758 |
| Cfr9I | CCCGGG | 1 cut(s) | 580 |
| CseI | GACGC | 1 cut(s) | 416 |
| Csp6I | GTAC | 1 cut(s) | 1854 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1666, 1701 |
| CviAII | CATG | 8 cut(s) | 171, 257, 524, 668, 963, 988, 1574, 1582 |
| CviQI | GTAC | 1 cut(s) | 1854 |
| DdeI | CTNAG | 6 cut(s) | 73, 611, 1239, 1248, 1707, 2105 |
| DpnI | GATC | 5 cut(s) | 10, 1572, 1743, 2168, 2205 |
| DpnII | GATC | 5 cut(s) | 8, 1570, 1741, 2166, 2203 |
| DraIII | CACNNNGTG | 1 cut(s) | 473 |
| EaeI | YGGCCR | 1 cut(s) | 1454 |
| Eam1104I | CTCTTC | 4 cut(s) | 627, 669, 1178, 1690 |
| EarI | CTCTTC | 4 cut(s) | 627, 669, 1178, 1690 |
| EciI | GGCGGA | 1 cut(s) | 74 |
| Eco24I | GRGCYC | 1 cut(s) | 1966 |
| Eco31I | GGTCTC | 1 cut(s) | 1625 |
| Eco47I | GGWCC | 2 cut(s) | 1221, 1758 |
| Eco88I | CYCGRG | 1 cut(s) | 580 |
| Eco91I | GGTNACC | 2 cut(s) | 584, 1004 |
| EcoO109I | RGGNCCY | 1 cut(s) | 819 |
| EcoO65I | GGTNACC | 2 cut(s) | 584, 1004 |
| EcoRI | GAATTC | 1 cut(s) | 178 |
| EcoRII | CCWGG | 2 cut(s) | 1126, 2266 |
| EcoT22I | ATGCAT | 2 cut(s) | 258, 851 |
| EcoT38I | GRGCYC | 1 cut(s) | 1966 |
| FaeI | CATG | 8 cut(s) | 174, 260, 527, 671, 966, 991, 1577, 1585 |
| FaqI | GGGAC | 1 cut(s) | 56 |
| FatI | CATG | 8 cut(s) | 170, 256, 523, 667, 962, 987, 1573, 1581 |
| FauI | CCCGC | 3 cut(s) | 264, 910, 2215 |
| FbaI | TGATCA | 1 cut(s) | 1570 |
| Fnu4HI | GCNGC | 4 cut(s) | 213, 350, 915, 1518 |
| FriOI | GRGCYC | 1 cut(s) | 1966 |
| Fsp4HI | GCNGC | 4 cut(s) | 213, 350, 915, 1518 |
| FspBI | CTAG | 7 cut(s) | 492, 1137, 1800, 1892, 1916, 2004, 2118 |
| GluI | GCNGC | 4 cut(s) | 213, 350, 915, 1518 |
| GsuI | CTGGAG | 1 cut(s) | 2250 |
| HaeIII | GGCC | 9 cut(s) | 328, 349, 364, 579, 821, 943, 1126, 1445, 1456 |
| HapII | CCGG | 5 cut(s) | 510, 581, 816, 1745, 1833 |
| HgaI | GACGC | 1 cut(s) | 416 |
| Hin1II | CATG | 8 cut(s) | 174, 260, 527, 671, 966, 991, 1577, 1585 |
| HincII | GTYRAC | 2 cut(s) | 431, 841 |
| HindII | GTYRAC | 2 cut(s) | 431, 841 |
| HindIII | AAGCTT | 1 cut(s) | 1168 |
| HinfI | GANTC | 7 cut(s) | 398, 797, 982, 1131, 1866, 2051, 2081 |
| HpaII | CCGG | 5 cut(s) | 510, 581, 816, 1745, 1833 |
| HphI | GGTGA | 3 cut(s) | 1370, 1657, 2048 |
| Hpy166II | GTNNAC | 2 cut(s) | 431, 841 |
| Hpy188I | TCNGA | 2 cut(s) | 2056, 2208 |
| Hpy8I | GTNNAC | 2 cut(s) | 431, 841 |
| Hpy99I | CGWCG | 1 cut(s) | 2251 |
| HpyAV | CCTTC | 9 cut(s) | 256, 317, 451, 622, 761, 1030, 1580, 1953, 2220 |
| HpyCH4III | ACNGT | 2 cut(s) | 1273, 2251 |
| HpyCH4IV | ACGT | 1 cut(s) | 2211 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 56, 197, 768, 2009 |
| HpyF3I | CTNAG | 6 cut(s) | 73, 611, 1239, 1248, 1707, 2105 |
| HpySE526I | ACGT | 1 cut(s) | 2211 |
| Hsp92II | CATG | 8 cut(s) | 174, 260, 527, 671, 966, 991, 1577, 1585 |
| KroI | GCCGGC | 1 cut(s) | 509 |
| KroNI | GCCGGC | 1 cut(s) | 511 |
| Ksp22I | TGATCA | 1 cut(s) | 1570 |
| Kzo9I | GATC | 5 cut(s) | 8, 1570, 1741, 2166, 2203 |
| LguI | GCTCTTC | 1 cut(s) | 1690 |
| LmnI | GCTCC | 2 cut(s) | 224, 1330 |
| LpnPI | CCDG | 8 cut(s) | 523, 594, 829, 1113, 1140, 1758, 1846, 2253 |
| Lsp1109I | GCAGC | 1 cut(s) | 224 |
| LweI | GCATC | 9 cut(s) | 52, 178, 389, 414, 436, 487, 700, 811, 1885 |
| MaeI | CTAG | 7 cut(s) | 492, 1137, 1800, 1892, 1916, 2004, 2118 |
| MaeII | ACGT | 1 cut(s) | 2211 |
| MaeIII | GTNAC | 7 cut(s) | 584, 719, 886, 1004, 1132, 1300, 1645 |
| MalI | GATC | 5 cut(s) | 10, 1572, 1743, 2168, 2205 |
| MboI | GATC | 5 cut(s) | 8, 1570, 1741, 2166, 2203 |
| MfeI | CAATTG | 1 cut(s) | 405 |
| MhlI | GDGCHC | 3 cut(s) | 868, 1283, 1966 |
| MlyI | GAGTC | 3 cut(s) | 1140, 2045, 2090 |
| MmeI | TCCRAC | 3 cut(s) | 16, 620, 1729 |
| Mph1103I | ATGCAT | 2 cut(s) | 258, 851 |
| MroNI | GCCGGC | 1 cut(s) | 509 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1560 |
| MseI | TTAA | 7 cut(s) | 234, 441, 756, 1335, 1419, 1817, 1947 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1834, 2199 |
| MspI | CCGG | 5 cut(s) | 510, 581, 816, 1745, 1833 |
| MspR9I | CCNGG | 6 cut(s) | 581, 582, 817, 1128, 1834, 2268 |
| MunI | CAATTG | 1 cut(s) | 405 |
| Mva1269I | GAATGC | 3 cut(s) | 513, 1046, 1427 |
| MvaI | CCWGG | 2 cut(s) | 1128, 2268 |
| MvnI | CGCG | 1 cut(s) | 2222 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 56, 197, 768, 2009 |
| NaeI | GCCGGC | 1 cut(s) | 511 |
| NciI | CCSGG | 4 cut(s) | 581, 582, 817, 1834 |
| NdeII | GATC | 5 cut(s) | 8, 1570, 1741, 2166, 2203 |
| NgoMIV | GCCGGC | 1 cut(s) | 509 |
| NheI | GCTAGC | 1 cut(s) | 2003 |
| NlaIII | CATG | 8 cut(s) | 174, 260, 527, 671, 966, 991, 1577, 1585 |
| NlaIV | GGNNCC | 1 cut(s) | 820 |
| NmuCI | GTSAC | 4 cut(s) | 719, 886, 1132, 1645 |
| NsiI | ATGCAT | 2 cut(s) | 258, 851 |
| PciSI | GCTCTTC | 1 cut(s) | 1690 |
| PctI | GAATGC | 3 cut(s) | 513, 1046, 1427 |
| PdiI | GCCGGC | 1 cut(s) | 511 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1560 |
| PfeI | GAWTC | 4 cut(s) | 398, 797, 982, 1866 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1642 |
| PkrI | GCNGC | 4 cut(s) | 214, 351, 916, 1519 |
| PleI | GAGTC | 3 cut(s) | 1139, 2045, 2089 |
| PpsI | GAGTC | 3 cut(s) | 1139, 2045, 2089 |
| PshBI | ATTAAT | 1 cut(s) | 441 |
| Psp6I | CCWGG | 2 cut(s) | 1126, 2266 |
| PspEI | GGTNACC | 2 cut(s) | 584, 1004 |
| PspGI | CCWGG | 2 cut(s) | 1126, 2266 |
| PspN4I | GGNNCC | 1 cut(s) | 820 |
| PspPI | GGNCC | 6 cut(s) | 326, 362, 578, 819, 1221, 1758 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1523, 1555 |
| RsaI | GTAC | 1 cut(s) | 1855 |
| RsaNI | GTAC | 1 cut(s) | 1854 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1834, 2199 |
| SapI | GCTCTTC | 1 cut(s) | 1690 |
| SaqAI | TTAA | 7 cut(s) | 234, 441, 756, 1335, 1419, 1817, 1947 |
| SatI | GCNGC | 4 cut(s) | 213, 350, 915, 1518 |
| Sau3AI | GATC | 5 cut(s) | 8, 1570, 1741, 2166, 2203 |
| Sau96I | GGNCC | 6 cut(s) | 326, 362, 578, 819, 1221, 1758 |
| ScaI | AGTACT | 1 cut(s) | 1855 |
| SchI | GAGTC | 3 cut(s) | 1140, 2045, 2090 |
| ScrFI | CCNGG | 6 cut(s) | 581, 582, 817, 1128, 1834, 2268 |
| SduI | GDGCHC | 3 cut(s) | 868, 1283, 1966 |
| SfaNI | GCATC | 9 cut(s) | 52, 178, 389, 414, 436, 487, 700, 811, 1885 |
| SinI | GGWCC | 2 cut(s) | 1221, 1758 |
| SmaI | CCCGGG | 1 cut(s) | 582 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1834, 2199 |
| SmlI | CTYRAG | 5 cut(s) | 659, 1400, 1628, 1965, 2013 |
| SmoI | CTYRAG | 5 cut(s) | 659, 1400, 1628, 1965, 2013 |
| SsiI | CCGC | 9 cut(s) | 50, 59, 271, 350, 914, 917, 1224, 1517, 2222 |
| SspMI | CTAG | 7 cut(s) | 492, 1137, 1800, 1892, 1916, 2004, 2118 |
| StyD4I | CCNGG | 6 cut(s) | 579, 580, 815, 1126, 1832, 2266 |
| TaaI | ACNGT | 2 cut(s) | 1273, 2251 |
| TaiI | ACGT | 1 cut(s) | 2214 |
| TaqII | GACCGA | 2 cut(s) | 722, 738 |
| TatI | WGTACW | 1 cut(s) | 1853 |
| TauI | GCSGC | 3 cut(s) | 352, 917, 1520 |
| TfiI | GAWTC | 4 cut(s) | 398, 797, 982, 1866 |
| Tru1I | TTAA | 7 cut(s) | 234, 441, 756, 1335, 1419, 1817, 1947 |
| Tru9I | TTAA | 7 cut(s) | 234, 441, 756, 1335, 1419, 1817, 1947 |
| TseFI | GTSAC | 4 cut(s) | 719, 886, 1132, 1645 |
| TseI | GCWGC | 1 cut(s) | 212 |
| Tsp45I | GTSAC | 4 cut(s) | 719, 886, 1132, 1645 |
| TspGWI | ACGGA | 2 cut(s) | 200, 905 |
| TspMI | CCCGGG | 1 cut(s) | 580 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1642 |
| VpaK11BI | GGWCC | 2 cut(s) | 1221, 1758 |
| VspI | ATTAAT | 1 cut(s) | 441 |
| XapI | RAATTY | 4 cut(s) | 178, 652, 922, 1016 |
| XbaI | TCTAGA | 1 cut(s) | 2117 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 951 |
| XmaI | CCCGGG | 1 cut(s) | 580 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1560 |
| XspI | CTAG | 7 cut(s) | 492, 1137, 1800, 1892, 1916, 2004, 2118 |
| ZrmI | AGTACT | 1 cut(s) | 1855 |
| Zsp2I | ATGCAT | 2 cut(s) | 258, 851 |