Rw4G032430

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000698
Physical Location & Seq
Reverse (-)
59569798 .. 59571302
1505 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G032430.1

Sequence Viewer

Length: 1281 bp
ATGGTTCAAGGAATTGGCAACCAACCTAGATTCAAATGGGTGATTGAGAGGGCAAAGAGCTTCACCATCTATATTTATGCACATCACAAGACTTTGGCATTGATGAGGAAGTTTACAAGGAAAAGAGATATAGTGAGGCCGGGAGTCACTAGATTTGCAACAGCTTTCCTCACTTTGCAAAGCTTGATGGAGAAGAAGAATGAGTTGAGAGCTATGATTACTAGTGATGAATGGAATGAAAGCAAGCATGCAAAGAGTGTAAAGGGGAAGGCGGCAGTGAATATTTCTTTGAGTGCTTCTTTTTGGAATGGGGTAAGTCTTTGCTTGAAGGTGTTTGCCCCTTTAGTCAAGGTGCTTCGCCTTGTTGATGGGGATAGAAAGCCATCAATGGGCTTTGTGTATGGAGAACTACTTAGAGTCAAGGAGGAGATTAAAATGGCATTCAAAGATCAAGAAACTCACTATCGTCCCATCCTTGACATTGTTGATGGAAAAGCTCGTGGTCGACTTGATAGTCCATTGCATTTAGCGGGTTACCTCTTGAACCCTTACTACACATATGCCAATCCAAGTCTTGAGAATCATAATGTGGTCATGGATGGGTTCTTCACTTGTGTTGAGACATTCTTTCCTGATGACATTCAAACTCAAAGTTTGGTGACAAATGTAGAATTGCACAAGTATTTGAAGAAAGATGATGGATTTGGAAGAGGTTTGGCTAAGGCGGGATGCGCACAAAATGATGACACTTATAATCCGGTTTTGTGGTGGAATATTTATGGAAACCTTGTACCAAGATTGCAAAGTATGGCTAAAAGGATACTTTCATTGACCACAAGCTCATCCGGATGTGAGAGAAATTGGAGCGCTTTTGAGGGGATCCATACAAAGAAAAGGAATAGACTAGATACGACGAGGTTGAACAATTTAGTTTATGTCCAATTGAATGCCAAGATCATCAACAAGAAGAGAAGAATGAAAGAGAAGAATGTGGATGTATTACTAGCATGTGAAGCTACTATGGCCCAAGGATGGATTGTGGATGGTAGTGATGAAGATGTAGATTCCGATCTTACTAGTGAAGTAGTTGGAGAGGGATCGGGACTGGGAGTGGATAGTAGCTTAGAGCATAGGAGAAGTAGTAGAATTCAAGAAATTAGGGAACTTCATGAGGAGGATTTTGTATCTGATGAAGAGGAAGAAGATGAGATGACTTTTGAGTTTGAGTCCGATGAGGAGGGAGTACTAGAGGGATATGGAGAAGAAGAATTTGAGGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659 Dimer_Tnp_hAT

Protein Analysis

426

Amino Acids

48.59

Weight (kDa)

5.81

Isoelectric Point (pI)

42.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimer_Tnp_hAT PF05699 244 - 315 5.1e-12 hAT family C-terminal dimerisation region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000698)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G43260 AT5G31412
fragaria_vesca FvH4_2g24751 FvH4_3g29751 FvH4_3g29752 FvH4_3g32705 FvH4_4g20841 FvH4_5g39742 FvH4_6g06672 FvH4_6g28272 FvH4_6g28273 FvH4_7g00215
malus_domestica MD07G1147000.v1.1
prunus_persica Prupe.4G255800_v2.0.a1
pyrus_communis pycom09g15950 pycom10g13140
rosa_chinensis RchiOBHm_Chr1g0314441 RchiOBHm_Chr1g0314451 RchiOBHm_Chr4g0430441 RchiOBHm_Chr4g0430831 RchiOBHm_Chr5g0003001 RchiOBHm_Chr5g0033441 RchiOBHm_Chr6g0251571 RchiOBHm_Chr6g0288501
rosa_multiflora Rmu_co8007098.1_g000001 Rmu_co8211970.1_g000002 Rmu_sc0000146.1_g000031 Rmu_sc0000555.1_g000022 Rmu_sc0000968.1_g000005 Rmu_sc0001017.1_g000028 Rmu_sc0001208.1_g000044 Rmu_sc0001526.1_g000038 Rmu_sc0002226.1_g000026 Rmu_sc0002284.1_g000006 Rmu_sc0002776.1_g000004 Rmu_sc0003369.1_g000011 Rmu_sc0003632.1_g000005 Rmu_sc0004180.1_g000007 Rmu_sc0004180.1_g000008 Rmu_sc0005014.1_g000005 Rmu_sc0005023.1_g000011 Rmu_sc0007296.1_g000009 Rmu_sc0011153.1_g000002 Rmu_sc0011153.1_g000003 Rmu_sc0018472.1_g000003 Rmu_sc0020646.1_g000001 Rmu_sc0022773.1_g000001 Rmu_sc0022773.1_g000002 Rmu_sc0023555.1_g000001 Rmu_sc0023788.1_g000001 Rmu_ssc0000409.1_g000026 Rmu_ssc0000421.1_g000039
rosa_roxburghii Rroxscaffold_2G00091530 Rroxscaffold_5G00355490 Rroxscaffold_6G00405690 Rroxscaffold_6G00412290 Rroxscaffold_7G00195870
rosa_rugosa Rorug05G0244000
rosa_samantha Rh3DG075100
rosa_wichuraiana Rw0G014340 Rw1G023020 Rw2G020120 Rw2G033790 Rw2G035670 Rw3G022290 Rw3G023070 Rw3G023080 Rw4G003460 Rw4G017840 Rw4G032430 Rw5G024280 Rw5G044440 Rw6G027410 Rw7G020330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 753
AasI GACNNNNNNGTC 1 cut(s) 513
Acc16I TGCGCA 1 cut(s) 733
AccI GTMKAC 1 cut(s) 505
AccIII TCCGGA 1 cut(s) 845
AciI CCGC 3 cut(s) 272, 530, 725
AclWI GGATC 3 cut(s) 874, 887, 1105
AcsI RAATTY 2 cut(s) 1146, 1268
AfaI GTAC 2 cut(s) 792, 1245
AfeI AGCGCT 1 cut(s) 868
AfiI CCNNNNNNNGG 2 cut(s) 389, 1032
AhlI ACTAGT 2 cut(s) 221, 1076
AluBI AGCT 8 cut(s) 60, 164, 183, 212, 497, 840, 1016, 1122
AluI AGCT 8 cut(s) 60, 164, 183, 212, 497, 840, 1016, 1122
Alw26I GTCTC 1 cut(s) 614
AlwI GGATC 3 cut(s) 874, 887, 1105
Aor13HI TCCGGA 1 cut(s) 845
Aor51HI AGCGCT 1 cut(s) 868
AoxI GGCC 2 cut(s) 137, 1023
ApoI RAATTY 2 cut(s) 1146, 1268
AspLEI GCGC 2 cut(s) 734, 869
AspS9I GGNCC 1 cut(s) 1024
AsuC2I CCSGG 1 cut(s) 141
AsuHPI GGTGA 3 cut(s) 52, 55, 670
BamHI GGATCC 1 cut(s) 879
BauI CACGAG 1 cut(s) 498
BciVI GTATCC 1 cut(s) 813
BcnI CCSGG 1 cut(s) 141
BcoDI GTCTC 1 cut(s) 614
BcuI ACTAGT 2 cut(s) 221, 1076
BfaI CTAG 7 cut(s) 27, 150, 222, 905, 1004, 1077, 1247
BfoI RGCGCY 1 cut(s) 870
BfuI GTATCC 1 cut(s) 813
BisI GCNGC 1 cut(s) 273
BlsI GCNGC 1 cut(s) 274
BmcAI AGTACT 1 cut(s) 1245
Bme1390I CCNGG 1 cut(s) 141
BmgT120I GGNCC 1 cut(s) 1024
BmiI GGNNCC 1 cut(s) 881
BmrFI CCNGG 1 cut(s) 141
BmrI ACTGGG 1 cut(s) 1115
BmsI GCATC 1 cut(s) 719
BmuI ACTGGG 1 cut(s) 1115
Bpu10I CCTNAGC 1 cut(s) 720
BpuEI CTTGAG 1 cut(s) 596
BpuMI CCSGG 1 cut(s) 141
BsaBI GATNNNNATC 1 cut(s) 1068
BsaJI CCNNGG 1 cut(s) 1027
BsaWI WCCGGW 2 cut(s) 757, 845
BsaXI ACNNNNNCTCC 2 cut(s) 1126, 1156
Bsc4I CCNNNNNNNGG 2 cut(s) 389, 1032
Bse1I ACTGG 1 cut(s) 1110
Bse3DI GCAATG 1 cut(s) 518
Bse8I GATNNNNATC 1 cut(s) 1068
BseAI TCCGGA 1 cut(s) 845
BseDI CCNNGG 1 cut(s) 1027
BseGI GGATG 8 cut(s) 471, 604, 734, 842, 854, 1000, 1037, 1048
BseJI GATNNNNATC 1 cut(s) 1068
BseLI CCNNNNNNNGG 2 cut(s) 389, 1032
BseMI GCAATG 1 cut(s) 518
BseNI ACTGG 1 cut(s) 1110
BseRI GAGGAG 3 cut(s) 440, 1187, 1250
BshFI GGCC 2 cut(s) 139, 1025
BsiSI CCGG 3 cut(s) 140, 758, 846
BslFI GGGAC 2 cut(s) 453, 1116
BslI CCNNNNNNNGG 2 cut(s) 389, 1032
BsmAI GTCTC 1 cut(s) 614
BsmFI GGGAC 2 cut(s) 453, 1116
BsmI GAATGC 2 cut(s) 440, 952
BsnI GGCC 2 cut(s) 139, 1025
Bsp13I TCCGGA 1 cut(s) 845
Bsp143I GATC 5 cut(s) 448, 879, 954, 1069, 1097
BspACI CCGC 3 cut(s) 272, 530, 725
BspANI GGCC 2 cut(s) 139, 1025
BspEI TCCGGA 1 cut(s) 845
BspHI TCATGA 1 cut(s) 1168
BspLI GGNNCC 1 cut(s) 881
BspPI GGATC 3 cut(s) 874, 887, 1105
BsrDI GCAATG 1 cut(s) 518
BsrI ACTGG 1 cut(s) 1110
BssECI CCNNGG 1 cut(s) 1027
BssMI GATC 5 cut(s) 448, 879, 954, 1069, 1097
BssSI CACGAG 1 cut(s) 498
BssT1I CCWWGG 1 cut(s) 1027
Bst2BI CACGAG 1 cut(s) 498
Bst6I CTCTTC 3 cut(s) 703, 962, 1188
BstC8I GCNNGC 2 cut(s) 245, 249
BstDEI CTNAG 3 cut(s) 413, 720, 1123
BstEII GGTNACC 1 cut(s) 533
BstF5I GGATG 8 cut(s) 471, 604, 734, 842, 854, 1000, 1037, 1048
BstH2I RGCGCY 1 cut(s) 870
BstHHI GCGC 2 cut(s) 734, 869
BstKTI GATC 5 cut(s) 451, 882, 957, 1072, 1100
BstMAI GTCTC 1 cut(s) 614
BstMBI GATC 5 cut(s) 448, 879, 954, 1069, 1097
BstMWI GCNNNNNNNGC 3 cut(s) 731, 1013, 1022
BstNSI RCATGY 2 cut(s) 251, 1011
BstPI GGTNACC 1 cut(s) 533
BstSCI CCNGG 1 cut(s) 139
BstX2I RGATCY 1 cut(s) 879
BstYI RGATCY 1 cut(s) 879
BsuI GTATCC 1 cut(s) 813
BsuRI GGCC 2 cut(s) 139, 1025
BtsCI GGATG 8 cut(s) 471, 604, 734, 842, 854, 1000, 1037, 1048
BtsI GCAGTG 1 cut(s) 282
BtsIMutI CAGTG 1 cut(s) 282
Cac8I GCNNGC 2 cut(s) 245, 249
CciI TCATGA 1 cut(s) 1168
CfoI GCGC 2 cut(s) 734, 869
Cfr13I GGNCC 1 cut(s) 1024
Csp6I GTAC 2 cut(s) 791, 1244
CviAII CATG 4 cut(s) 248, 595, 1008, 1169
CviQI GTAC 2 cut(s) 791, 1244
DdeI CTNAG 3 cut(s) 413, 720, 1123
DpnI GATC 5 cut(s) 450, 881, 956, 1071, 1099
DpnII GATC 5 cut(s) 448, 879, 954, 1069, 1097
DrdI GACNNNNNNGTC 1 cut(s) 513
DseDI GACNNNNNNGTC 1 cut(s) 513
Eam1104I CTCTTC 3 cut(s) 703, 962, 1188
EarI CTCTTC 3 cut(s) 703, 962, 1188
Eco130I CCWWGG 1 cut(s) 1027
Eco47III AGCGCT 1 cut(s) 868
Eco91I GGTNACC 1 cut(s) 533
EcoO65I GGTNACC 1 cut(s) 533
EcoRI GAATTC 1 cut(s) 1146
EcoT14I CCWWGG 1 cut(s) 1027
ErhI CCWWGG 1 cut(s) 1027
FaeI CATG 4 cut(s) 251, 598, 1011, 1172
FaqI GGGAC 2 cut(s) 453, 1116
FatI CATG 4 cut(s) 247, 594, 1007, 1168
FauI CCCGC 2 cut(s) 523, 718
FauNDI CATATG 1 cut(s) 559
FblI GTMKAC 1 cut(s) 505
Fnu4HI GCNGC 1 cut(s) 273
FokI GGATG 8 cut(s) 458, 611, 741, 829, 861, 1007, 1044, 1055
Fsp4HI GCNGC 1 cut(s) 273
FspAI RTGCGCAY 1 cut(s) 733
FspBI CTAG 7 cut(s) 27, 150, 222, 905, 1004, 1077, 1247
FspI TGCGCA 1 cut(s) 733
GlaI GCGC 2 cut(s) 733, 868
GluI GCNGC 1 cut(s) 273
HaeII RGCGCY 1 cut(s) 870
HaeIII GGCC 2 cut(s) 139, 1025
HapII CCGG 3 cut(s) 140, 758, 846
HhaI GCGC 2 cut(s) 734, 869
Hin1II CATG 4 cut(s) 251, 598, 1011, 1172
Hin6I GCGC 2 cut(s) 732, 867
HinP1I GCGC 2 cut(s) 732, 867
HincII GTYRAC 1 cut(s) 506
HindII GTYRAC 1 cut(s) 506
HindIII AAGCTT 1 cut(s) 181
HinfI GANTC 6 cut(s) 30, 144, 417, 580, 1064, 1226
HpaII CCGG 3 cut(s) 140, 758, 846
HphI GGTGA 3 cut(s) 52, 55, 670
Hpy166II GTNNAC 2 cut(s) 114, 506
Hpy188I TCNGA 3 cut(s) 1069, 1189, 1231
Hpy188III TCNNGA 8 cut(s) 452, 541, 575, 632, 846, 1101, 1151, 1169
Hpy8I GTNNAC 2 cut(s) 114, 506
Hpy99I CGWCG 1 cut(s) 916
HpyAV CCTTC 2 cut(s) 262, 322
HpyCH4V TGCA 7 cut(s) 80, 158, 178, 251, 523, 676, 802
HpyF10VI GCNNNNNNNGC 3 cut(s) 731, 1013, 1022
HpyF3I CTNAG 3 cut(s) 413, 720, 1123
Hsp92II CATG 4 cut(s) 251, 598, 1011, 1172
HspAI GCGC 2 cut(s) 732, 867
Kpn2I TCCGGA 1 cut(s) 845
Kzo9I GATC 5 cut(s) 448, 879, 954, 1069, 1097
LmnI GCTCC 1 cut(s) 864
LpnPI CCDG 5 cut(s) 153, 645, 771, 859, 1091
LweI GCATC 1 cut(s) 719
MaeI CTAG 7 cut(s) 27, 150, 222, 905, 1004, 1077, 1247
MaeIII GTNAC 3 cut(s) 145, 533, 658
MalI GATC 5 cut(s) 450, 881, 956, 1071, 1099
MboI GATC 5 cut(s) 448, 879, 954, 1069, 1097
MfeI CAATTG 1 cut(s) 941
MflI RGATCY 1 cut(s) 879
MluCI AATT 8 cut(s) 12, 671, 859, 925, 941, 1146, 1155, 1268
MlyI GAGTC 3 cut(s) 153, 426, 1235
MmeI TCCRAC 1 cut(s) 1069
MroI TCCGGA 1 cut(s) 845
MseI TTAA 1 cut(s) 432
MslI CAYNNNNRTG 1 cut(s) 847
MspI CCGG 3 cut(s) 140, 758, 846
MspR9I CCNGG 1 cut(s) 141
MunI CAATTG 1 cut(s) 941
Mva1269I GAATGC 2 cut(s) 440, 952
MwoI GCNNNNNNNGC 3 cut(s) 731, 1013, 1022
NciI CCSGG 1 cut(s) 141
NdeI CATATG 1 cut(s) 559
NdeII GATC 5 cut(s) 448, 879, 954, 1069, 1097
NlaIII CATG 4 cut(s) 251, 598, 1011, 1172
NlaIV GGNNCC 1 cut(s) 881
NmuCI GTSAC 2 cut(s) 145, 658
NsbI TGCGCA 1 cut(s) 733
NspI RCATGY 2 cut(s) 251, 1011
PaeI GCATGC 1 cut(s) 251
PagI TCATGA 1 cut(s) 1168
PctI GAATGC 2 cut(s) 440, 952
PfeI GAWTC 3 cut(s) 30, 580, 1064
PkrI GCNGC 1 cut(s) 274
PleI GAGTC 3 cut(s) 152, 425, 1234
PpsI GAGTC 3 cut(s) 152, 425, 1234
PsiI TTATAA 1 cut(s) 753
PspEI GGTNACC 1 cut(s) 533
PspN4I GGNNCC 1 cut(s) 881
PspPI GGNCC 1 cut(s) 1024
PsrI GAACNNNNNNTAC 2 cut(s) 536, 568
PsuI RGATCY 1 cut(s) 879
RsaI GTAC 2 cut(s) 792, 1245
RsaNI GTAC 2 cut(s) 791, 1244
RseI CAYNNNNRTG 1 cut(s) 847
SalI GTCGAC 1 cut(s) 504
SaqAI TTAA 1 cut(s) 432
SatI GCNGC 1 cut(s) 273
Sau3AI GATC 5 cut(s) 448, 879, 954, 1069, 1097
Sau96I GGNCC 1 cut(s) 1024
ScaI AGTACT 1 cut(s) 1245
SchI GAGTC 3 cut(s) 153, 426, 1235
ScrFI CCNGG 1 cut(s) 141
SfaNI GCATC 1 cut(s) 719
SmiMI CAYNNNNRTG 1 cut(s) 847
SmlI CTYRAG 1 cut(s) 575
SmoI CTYRAG 1 cut(s) 575
SpeI ACTAGT 2 cut(s) 221, 1076
SphI GCATGC 1 cut(s) 251
Sse9I AATT 8 cut(s) 12, 671, 859, 925, 941, 1146, 1155, 1268
SsiI CCGC 3 cut(s) 272, 530, 725
SspI AATATT 2 cut(s) 283, 775
SspMI CTAG 7 cut(s) 27, 150, 222, 905, 1004, 1077, 1247
StyD4I CCNGG 1 cut(s) 139
StyI CCWWGG 1 cut(s) 1027
TaqI TCGA 1 cut(s) 505
TasI AATT 8 cut(s) 12, 671, 859, 925, 941, 1146, 1155, 1268
TatI WGTACW 1 cut(s) 1243
TauI GCSGC 1 cut(s) 275
TfiI GAWTC 3 cut(s) 30, 580, 1064
Tru1I TTAA 1 cut(s) 432
Tru9I TTAA 1 cut(s) 432
TscAI CASTG 1 cut(s) 282
TseFI GTSAC 2 cut(s) 145, 658
Tsp45I GTSAC 2 cut(s) 145, 658
TspDTI ATGAA 7 cut(s) 243, 252, 816, 992, 1068, 1157, 1206
TspRI CASTG 1 cut(s) 282
XapI RAATTY 2 cut(s) 1146, 1268
XceI RCATGY 2 cut(s) 251, 1011
XmiI GTMKAC 1 cut(s) 505
XspI CTAG 7 cut(s) 27, 150, 222, 905, 1004, 1077, 1247
ZrmI AGTACT 1 cut(s) 1245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.