AT1G27540

cysteine-rich repeat secretory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0000658
Physical Location & Seq
Reverse (-)
9565913 .. 9567906
1994 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G27540.1

Sequence Viewer

Length: 1242 bp
ATGGAGAGATCTACGCTCAAACGAACATCGAGTTTTCCAGATATTGAGGCTGTTCAGGGGTGTTCGACTTTGAATTTGCTGCCAAAAGCCGGATCTAGATCTACGCTCAAACGAACATCGAGTATTCCTGATTTTGAGCCTGTTAAGTCTCTGCGTTCGACTTTGATTCTGCAGCCAAAAGCCGGATCTCCATTGCTGATGCTGATTGCAGAAGAGTATGGTTGTCGTATTTACAACCCAGAAGATGGTAGGGTTTACGAGCCGAAGAGTGACTTTTCAGGGTATCGATTCCTGGGAAGCTCAGGTAAGTGGTTTCTGGTGGTAGATTCTCGAATGAAACTCTATATTATAGATGTATTTAGCGAGAAGAGGATCGATCTTCCACCACTAGAATCATTTAAGGGTAGCCATTTTAAGATTTGGCGTGAAGGAGAGGAGAATGTCAAGCATATGGTAATGGATGATGAGGATCCATTTTGTGATGAATTTCCTCATGCTGCCGAAGATCTGAGAGGTCGTTTGTGGGTTGACGACAAGAATGGAAACTACGTTGTTGTGTGGCAGTTTGAAATGTACCCATTTCTAGGGTTTTGCAGGACAGGGGATGATCATTACCGCGAGATTCCAACAGAGCTCGAGGTTCAGAGGGAGTTACGAGGTTTAAACGATATGGTACTGAAAGGTTACAATCTTTACGTCTATGCCACCCGTGAGTTCATTCGACACTTAGATTTGTCTGGACAAGATGAAGATGGTTTCAAGGATGTATCCGAGAAACATAGGCTTCCAATGTGGATGCCAAGTATGACAATGAAGGAACGTGAACGTATGAAGCAAAAGAGGTCTATCCCATCTAACAAAGGCATTGCTGTTACAAGAGCAGGTCAAGTTTTGGTTGTCTTTACGTACGATTTAGGTACCTCTGAAAGTCACAGGATGTTCCATCTGTACAAGAGGGATCCTAAAGATCTTGACCCGAAAACCCTCGACACCCGGCTTCTTGAGGTACATTCTCTAGGTGATGAGGCCCTCTTCTATGATTTGGGTATTACCGTGCCTGCTGACCATACCCTTGGAATTGAGCCTAACTCCATTTATTTCACTCGTGATGATCGTATCTGTCACAATAAATTGTCATGCCTCGACATCGGTGTCTTCAATCTTGCAACAAAGACCGTCAAACGCTTCCACGGTCTCTCCAACTTCAACTTCAACGATGCTCAGTGGTTTCTTCCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stress-antifung

Protein Analysis

413

Amino Acids

47.81

Weight (kDa)

6.1

Isoelectric Point (pI)

43.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 78 - 387 1e-40 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1151
Acc36I ACCTGC 1 cut(s) 872
Acc65I GGTACC 1 cut(s) 917
AccB1I GGYRCC 1 cut(s) 917
AccB7I CCANNNNNTGG 1 cut(s) 245
AccII CGCG 1 cut(s) 618
AciI CCGC 1 cut(s) 616
AclWI GGATC 7 cut(s) 100, 193, 380, 464, 477, 953, 966
AcsI RAATTY 2 cut(s) 73, 485
AfaI GTAC 6 cut(s) 575, 675, 908, 919, 950, 1008
AfiI CCNNNNNNNGG 4 cut(s) 89, 182, 245, 584
AgsI TTSAA 6 cut(s) 73, 569, 760, 1159, 1207, 1213
AjnI CCWGG 1 cut(s) 291
AjuI GAANNNNNNNTTGG 2 cut(s) 1193, 1225
AluBI AGCT 2 cut(s) 300, 634
AluI AGCT 2 cut(s) 300, 634
Alw21I GWGCWC 1 cut(s) 636
Alw26I GTCTC 2 cut(s) 153, 1199
AlwI GGATC 7 cut(s) 100, 193, 380, 464, 477, 953, 966
Ama87I CYCGRG 1 cut(s) 635
AoxI GGCC 1 cut(s) 1026
ApeKI GCWGC 3 cut(s) 79, 172, 497
ApoI RAATTY 2 cut(s) 73, 485
ArsI GACNNNNNNTTYG 2 cut(s) 58, 90
Asp718I GGTACC 1 cut(s) 917
AspS9I GGNCC 1 cut(s) 1027
AsuC2I CCSGG 1 cut(s) 994
AsuHPI GGTGA 1 cut(s) 1031
AvaI CYCGRG 1 cut(s) 635
BamHI GGATCC 2 cut(s) 469, 958
BanI GGYRCC 1 cut(s) 917
BanII GRGCYC 1 cut(s) 636
BauI CACGAG 1 cut(s) 1104
BbsI GAAGAC 1 cut(s) 1147
Bbv12I GWGCWC 1 cut(s) 636
BbvI GCAGC 3 cut(s) 66, 184, 484
BccI CCATC 4 cut(s) 239, 746, 859, 951
BciT130I CCWGG 1 cut(s) 293
BciVI GTATCC 1 cut(s) 778
BclI TGATCA 1 cut(s) 607
BcnI CCSGG 1 cut(s) 994
BcoDI GTCTC 2 cut(s) 153, 1199
BfaI CTAG 4 cut(s) 96, 389, 584, 1016
BfmI CTRYAG 1 cut(s) 170
BfuAI ACCTGC 1 cut(s) 872
BfuI GTATCC 1 cut(s) 778
BglII AGATCT 4 cut(s) 8, 98, 505, 967
BisI GCNGC 3 cut(s) 80, 173, 498
BlsI GCNGC 3 cut(s) 81, 174, 499
Bme1390I CCNGG 2 cut(s) 293, 994
BmeT110I CYCGRG 1 cut(s) 635
BmgT120I GGNCC 1 cut(s) 1027
BmiI GGNNCC 3 cut(s) 471, 919, 960
BmrFI CCNGG 2 cut(s) 293, 994
BmsI GCATC 3 cut(s) 189, 786, 1207
BpiI GAAGAC 1 cut(s) 1147
BplI GAGNNNNNCTC 2 cut(s) 1073, 1105
Bpu10I CCTNAGC 1 cut(s) 301
BpuEI CTTGAG 1 cut(s) 1022
BpuMI CCSGG 1 cut(s) 994
Bsa29I ATCGAT 2 cut(s) 286, 375
BsaAI YACGTR 1 cut(s) 906
BsaBI GATNNNNATC 2 cut(s) 97, 468
BsaI GGTCTC 1 cut(s) 1199
BsaJI CCNNGG 3 cut(s) 292, 1072, 1189
BsaXI ACNNNNNCTCC 2 cut(s) 1181, 1211
Bsc4I CCNNNNNNNGG 4 cut(s) 89, 182, 245, 584
Bse3DI GCAATG 2 cut(s) 191, 864
Bse8I GATNNNNATC 2 cut(s) 97, 468
BseBI CCWGG 1 cut(s) 293
BseCI ATCGAT 2 cut(s) 286, 375
BseDI CCNNGG 3 cut(s) 292, 1072, 1189
BseGI GGATG 5 cut(s) 466, 610, 769, 801, 942
BseJI GATNNNNATC 2 cut(s) 97, 468
BseLI CCNNNNNNNGG 4 cut(s) 89, 182, 245, 584
BseMI GCAATG 2 cut(s) 191, 864
BseMII CTCAG 3 cut(s) 315, 500, 1235
BseRI GAGGAG 1 cut(s) 449
BseXI GCAGC 3 cut(s) 66, 184, 484
Bsh1236I CGCG 1 cut(s) 618
BshFI GGCC 1 cut(s) 1028
BshNI GGYRCC 1 cut(s) 917
BshVI ATCGAT 2 cut(s) 286, 375
BsiHKAI GWGCWC 1 cut(s) 636
BsiHKCI CYCGRG 1 cut(s) 635
BsiSI CCGG 3 cut(s) 90, 183, 994
BsiWI CGTACG 1 cut(s) 906
BslI CCNNNNNNNGG 4 cut(s) 89, 182, 245, 584
BsmAI GTCTC 2 cut(s) 153, 1199
BsnI GGCC 1 cut(s) 1028
Bso31I GGTCTC 1 cut(s) 1199
BsoBI CYCGRG 1 cut(s) 635
Bsp1286I GDGCHC 1 cut(s) 636
Bsp1407I TGTACA 1 cut(s) 948
BspACI CCGC 1 cut(s) 616
BspANI GGCC 1 cut(s) 1028
BspCNI CTCAG 3 cut(s) 314, 501, 1234
BspDI ATCGAT 2 cut(s) 286, 375
BspFNI CGCG 1 cut(s) 618
BspLI GGNNCC 3 cut(s) 471, 919, 960
BspMAI CTGCAG 1 cut(s) 174
BspMI ACCTGC 1 cut(s) 872
BspPI GGATC 7 cut(s) 100, 193, 380, 464, 477, 953, 966
BspT107I GGYRCC 1 cut(s) 917
BspTNI GGTCTC 1 cut(s) 1199
BsrDI GCAATG 2 cut(s) 191, 864
BsrGI TGTACA 1 cut(s) 948
BssECI CCNNGG 3 cut(s) 292, 1072, 1189
BssSI CACGAG 1 cut(s) 1104
BssT1I CCWWGG 1 cut(s) 1072
Bst2BI CACGAG 1 cut(s) 1104
Bst2UI CCWGG 1 cut(s) 293
Bst4CI ACNGT 3 cut(s) 1054, 1177, 1193
Bst6I CTCTTC 4 cut(s) 207, 260, 362, 1037
BstAUI TGTACA 1 cut(s) 948
BstBAI YACGTR 1 cut(s) 906
BstC8I GCNNGC 1 cut(s) 1059
BstDEI CTNAG 4 cut(s) 301, 509, 727, 1221
BstDSI CCRYGG 1 cut(s) 1189
BstF5I GGATG 5 cut(s) 466, 610, 769, 801, 942
BstFNI CGCG 1 cut(s) 618
BstMAI GTCTC 2 cut(s) 153, 1199
BstNI CCWGG 1 cut(s) 293
BstSCI CCNGG 2 cut(s) 291, 992
BstSFI CTRYAG 1 cut(s) 170
BstSNI TACGTA 1 cut(s) 906
BstUI CGCG 1 cut(s) 618
BstV1I GCAGC 3 cut(s) 66, 184, 484
BstV2I GAAGAC 1 cut(s) 1147
BstX2I RGATCY 8 cut(s) 8, 92, 98, 185, 469, 505, 958, 967
BstXI CCANNNNNNTGG 1 cut(s) 1073
BstYI RGATCY 8 cut(s) 8, 92, 98, 185, 469, 505, 958, 967
Bsu15I ATCGAT 2 cut(s) 286, 375
BsuI GTATCC 1 cut(s) 778
BsuRI GGCC 1 cut(s) 1028
BsuTUI ATCGAT 2 cut(s) 286, 375
BtgI CCRYGG 1 cut(s) 1189
BtsCI GGATG 5 cut(s) 466, 610, 769, 801, 942
BtsIMutI CAGTG 1 cut(s) 1229
BveI ACCTGC 1 cut(s) 872
Cac8I GCNNGC 1 cut(s) 1059
Cfr13I GGNCC 1 cut(s) 1027
ClaI ATCGAT 2 cut(s) 286, 375
Csp6I GTAC 6 cut(s) 574, 674, 907, 918, 949, 1007
CviAII CATG 2 cut(s) 494, 1137
CviQI GTAC 6 cut(s) 574, 674, 907, 918, 949, 1007
DdeI CTNAG 4 cut(s) 301, 509, 727, 1221
DraI TTTAAA 1 cut(s) 663
DrdI GACNNNNNNGTC 1 cut(s) 1151
DseDI GACNNNNNNGTC 1 cut(s) 1151
Eam1104I CTCTTC 4 cut(s) 207, 260, 362, 1037
EarI CTCTTC 4 cut(s) 207, 260, 362, 1037
Ecl136II GAGCTC 1 cut(s) 634
Eco105I TACGTA 1 cut(s) 906
Eco130I CCWWGG 1 cut(s) 1072
Eco24I GRGCYC 1 cut(s) 636
Eco31I GGTCTC 1 cut(s) 1199
Eco53kI GAGCTC 1 cut(s) 634
Eco88I CYCGRG 1 cut(s) 635
EcoICRI GAGCTC 1 cut(s) 634
EcoO109I RGGNCCY 1 cut(s) 1027
EcoRII CCWGG 1 cut(s) 291
EcoT14I CCWWGG 1 cut(s) 1072
EcoT38I GRGCYC 1 cut(s) 636
ErhI CCWWGG 1 cut(s) 1072
FaeI CATG 2 cut(s) 497, 1140
FalI AAGNNNNNCTT 2 cut(s) 257, 289
FatI CATG 2 cut(s) 493, 1136
FauNDI CATATG 1 cut(s) 450
FbaI TGATCA 1 cut(s) 607
Fnu4HI GCNGC 3 cut(s) 80, 173, 498
FokI GGATG 5 cut(s) 473, 617, 776, 808, 949
FriOI GRGCYC 1 cut(s) 636
Fsp4HI GCNGC 3 cut(s) 80, 173, 498
FspBI CTAG 4 cut(s) 96, 389, 584, 1016
GluI GCNGC 3 cut(s) 80, 173, 498
HaeIII GGCC 1 cut(s) 1028
HapII CCGG 3 cut(s) 90, 183, 994
Hin1II CATG 2 cut(s) 497, 1140
HincII GTYRAC 1 cut(s) 529
HindII GTYRAC 1 cut(s) 529
HinfI GANTC 5 cut(s) 166, 288, 326, 392, 622
HpaII CCGG 3 cut(s) 90, 183, 994
HphI GGTGA 1 cut(s) 1031
Hpy166II GTNNAC 3 cut(s) 256, 529, 824
Hpy188I TCNGA 4 cut(s) 510, 645, 772, 925
Hpy188III TCNNGA 9 cut(s) 38, 96, 128, 330, 738, 971, 1001, 1106, 1239
Hpy8I GTNNAC 3 cut(s) 256, 529, 824
HpyAV CCTTC 2 cut(s) 422, 808
HpyCH4III ACNGT 3 cut(s) 1054, 1177, 1193
HpyCH4IV ACGT 5 cut(s) 549, 696, 820, 826, 905
HpyCH4V TGCA 4 cut(s) 172, 209, 594, 1166
HpyF3I CTNAG 4 cut(s) 301, 509, 727, 1221
HpySE526I ACGT 5 cut(s) 549, 696, 820, 826, 905
Hsp92II CATG 2 cut(s) 497, 1140
KpnI GGTACC 1 cut(s) 921
Ksp22I TGATCA 1 cut(s) 607
Lsp1109I GCAGC 3 cut(s) 66, 184, 484
LweI GCATC 3 cut(s) 189, 786, 1207
MaeI CTAG 4 cut(s) 96, 389, 584, 1016
MaeII ACGT 5 cut(s) 549, 696, 820, 826, 905
MaeIII GTNAC 6 cut(s) 269, 651, 683, 871, 929, 1121
MflI RGATCY 8 cut(s) 8, 92, 98, 185, 469, 505, 958, 967
MhlI GDGCHC 1 cut(s) 636
MluCI AATT 4 cut(s) 73, 485, 1077, 1130
MmeI TCCRAC 2 cut(s) 650, 1224
MseI TTAA 4 cut(s) 144, 399, 414, 662
MspI CCGG 3 cut(s) 90, 183, 994
MspR9I CCNGG 2 cut(s) 293, 994
MssI GTTTAAAC 1 cut(s) 663
MvaI CCWGG 1 cut(s) 293
MvnI CGCG 1 cut(s) 618
NciI CCSGG 1 cut(s) 994
NdeI CATATG 1 cut(s) 450
NlaIII CATG 2 cut(s) 497, 1140
NlaIV GGNNCC 3 cut(s) 471, 919, 960
NmuCI GTSAC 3 cut(s) 269, 929, 1121
PaeR7I CTCGAG 1 cut(s) 635
PcsI WCGNNNNNNNCGW 1 cut(s) 1111
PfeI GAWTC 5 cut(s) 166, 288, 326, 392, 622
Pfl23II CGTACG 1 cut(s) 906
PflMI CCANNNNNTGG 1 cut(s) 245
PkrI GCNGC 3 cut(s) 81, 174, 499
PmeI GTTTAAAC 1 cut(s) 663
Ppu21I YACGTR 1 cut(s) 906
Psp124BI GAGCTC 1 cut(s) 636
Psp6I CCWGG 1 cut(s) 291
PspGI CCWGG 1 cut(s) 291
PspLI CGTACG 1 cut(s) 906
PspN4I GGNNCC 3 cut(s) 471, 919, 960
PspPI GGNCC 1 cut(s) 1027
PspXI VCTCGAGB 1 cut(s) 635
PstI CTGCAG 1 cut(s) 174
PsuI RGATCY 8 cut(s) 8, 92, 98, 185, 469, 505, 958, 967
RsaI GTAC 6 cut(s) 575, 675, 908, 919, 950, 1008
RsaNI GTAC 6 cut(s) 574, 674, 907, 918, 949, 1007
SacI GAGCTC 1 cut(s) 636
SaqAI TTAA 4 cut(s) 144, 399, 414, 662
SatI GCNGC 3 cut(s) 80, 173, 498
Sau96I GGNCC 1 cut(s) 1027
ScrFI CCNGG 2 cut(s) 293, 994
SduI GDGCHC 1 cut(s) 636
SfaNI GCATC 3 cut(s) 189, 786, 1207
SfcI CTRYAG 1 cut(s) 170
Sfr274I CTCGAG 1 cut(s) 635
SlaI CTCGAG 1 cut(s) 635
SmlI CTYRAG 2 cut(s) 635, 1001
SmoI CTYRAG 2 cut(s) 635, 1001
SnaBI TACGTA 1 cut(s) 906
Sse9I AATT 4 cut(s) 73, 485, 1077, 1130
SsiI CCGC 1 cut(s) 616
SspMI CTAG 4 cut(s) 96, 389, 584, 1016
SstI GAGCTC 1 cut(s) 636
StyD4I CCNGG 2 cut(s) 291, 992
StyI CCWWGG 1 cut(s) 1072
TaaI ACNGT 3 cut(s) 1054, 1177, 1193
TaiI ACGT 5 cut(s) 552, 699, 823, 829, 908
TasI AATT 4 cut(s) 73, 485, 1077, 1130
TatI WGTACW 1 cut(s) 948
TfiI GAWTC 5 cut(s) 166, 288, 326, 392, 622
Tru1I TTAA 4 cut(s) 144, 399, 414, 662
Tru9I TTAA 4 cut(s) 144, 399, 414, 662
TscAI CASTG 1 cut(s) 1229
TseFI GTSAC 3 cut(s) 269, 929, 1121
TseI GCWGC 3 cut(s) 79, 172, 497
Tsp45I GTSAC 3 cut(s) 269, 929, 1121
TspDTI ATGAA 6 cut(s) 350, 498, 706, 762, 827, 845
TspRI CASTG 1 cut(s) 1229
Van91I CCANNNNNTGG 1 cut(s) 245
XapI RAATTY 2 cut(s) 73, 485
XbaI TCTAGA 1 cut(s) 95
XhoI CTCGAG 1 cut(s) 635
XspI CTAG 4 cut(s) 96, 389, 584, 1016
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.