AT5G66830

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000658
Physical Location & Seq
Forward (+)
26691253 .. 26692689
1437 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G66830.1

Sequence Viewer

Length: 1185 bp
ATGGCCTCTCCGCTACTCCCTGGTCCACCGGATCAGAGAAGAAATCTCGATTGGTGCTGGTCGAAGCTTCCTTCAGATCTCATGCAATTTGTATTTGACCGCCTTGGTTTTGCCGATTTCCAACGTGCAAAATCCGTTTGTTCATCTTGGCTTTCAGTTTCGAGAAATTCTCAGCCAAACAATCAAATCCCTTGGATGATTCGATTCCCCAAGGACAACAATCACTGTCTCTTGTTCAATCCTGAAGAGGAAGACAAGATGTACAAAACTCCAAATCTCGGCAACGATTTCGCAAAGAGCTCTTGTATCGCCAGTTATGGAAGCTGGCTTTTGATGCAACCTGAATCAGAATACATGGAAGAAGACTTAGACCATCAATGTAATAATCTCTATATTCTTGATCTTCTAACCCGCGAGAGAATCAATCTACCGATTCTCCAACCAGAGTTTGGACTCACATGTCCGATATTATGGACCGATGAGAAAAGCAAAGATCACCTAGTTATTGGAATGGCTCATGAAGAATTAGCCATTTCTTTCAAGAAAGGAGATTCCTCGTGGAAACAAATCCCAACCTTGTCAGGTATCGAAGAGTGTTTTAGCATGGTTTTTAAGGATCACAAACTTTACTGTCTGAGCAATTATAAACTCAAGGTCTTCGATTTCTCTGGAGATATTCCGGTCAAAGTTTTCAAAACTAGCGTAAGTAAGTTGTTAAATAATCCACTATGTATTTCCATGAGAATGAGACTTCCTGGAATTCCAATGAAGGACCAGTTGAATCACTTCAAGGATGATATGGTAGTCACTCTAGCCGGACATGTCTTAATTGTTAAGTGCCATCGTCCGAGTCTGTCCAAGATATGGAGCTTTGAAATCTACAAGATGGAAGGGAACAACAACAAGTGGGAGAAAACTGTTTCTCTGGGAGACGAGACAATTCTTTTGGATTTGGGTATCACAGTGCTCGCCAAGGACATGCAAGGGATCAAGGCCAATTCCATCTACTTCTCCAATCCTACTCCTTATTTTAAAGATCAATATGACGAAAACGAAATCTTCATATTCGACCTCGATTCCAACACCGTTGAACAACCGCACCGATCTGTTTCCTCTTCCTTTCCCCGCTCTAGAGCCCGATGGTTCTTGCCATGTTTCAAACGAGAATCATACTTCCTTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box

Protein Analysis

394

Amino Acids

45.74

Weight (kDa)

6.17

Isoelectric Point (pI)

46.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 20 - 58 1.1e-06 F-box domain
Beta-prop_KIB1-4 PF03478 79 - 357 3.6e-48 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 645
AasI GACNNNNNNGTC 1 cut(s) 459
AccB7I CCANNNNNTGG 2 cut(s) 449, 864
AccBSI CCGCTC 1 cut(s) 1128
AccII CGCG 1 cut(s) 414
AciI CCGC 5 cut(s) 11, 100, 412, 1097, 1126
AclWI GGATC 3 cut(s) 39, 624, 995
AcsI RAATTY 2 cut(s) 166, 759
AcuI CTGAAG 3 cut(s) 57, 264, 1163
AfaI GTAC 1 cut(s) 263
AfiI CCNNNNNNNGG 3 cut(s) 278, 449, 864
AflIII ACRYGT 2 cut(s) 458, 820
AgsI TTSAA 8 cut(s) 238, 541, 694, 781, 790, 875, 1091, 1159
AjnI CCWGG 2 cut(s) 19, 754
AjuI GAANNNNNNNTTGG 2 cut(s) 34, 66
AluBI AGCT 4 cut(s) 67, 300, 324, 870
AluI AGCT 4 cut(s) 67, 300, 324, 870
Alw21I GWGCWC 2 cut(s) 302, 969
Alw26I GTCTC 4 cut(s) 233, 742, 924, 929
AlwI GGATC 3 cut(s) 39, 624, 995
AoxI GGCC 2 cut(s) 3, 993
ApoI RAATTY 2 cut(s) 166, 759
ArsI GACNNNNNNTTYG 2 cut(s) 928, 960
Asp700I GAANNNNTTC 1 cut(s) 785
AspS9I GGNCC 3 cut(s) 23, 474, 772
AsuHPI GGTGA 1 cut(s) 488
AvaII GGWCC 3 cut(s) 23, 474, 772
BanII GRGCYC 2 cut(s) 302, 1138
BauI CACGAG 1 cut(s) 556
BbsI GAAGAC 3 cut(s) 258, 369, 649
Bbv12I GWGCWC 2 cut(s) 302, 969
BccI CCATC 5 cut(s) 381, 849, 880, 1010, 1134
BcgI CGANNNNNNTGC 2 cut(s) 271, 305
BciT130I CCWGG 2 cut(s) 21, 756
BcoDI GTCTC 4 cut(s) 233, 742, 924, 929
BfaI CTAG 4 cut(s) 500, 699, 812, 1131
BglII AGATCT 1 cut(s) 76
Bme1390I CCNGG 2 cut(s) 21, 756
Bme18I GGWCC 3 cut(s) 23, 474, 772
BmgT120I GGNCC 3 cut(s) 23, 474, 772
BmrFI CCNGG 2 cut(s) 21, 756
BmsI GCATC 1 cut(s) 324
BpiI GAAGAC 3 cut(s) 258, 369, 649
BplI GAGNNNNNCTC 2 cut(s) 154, 186
BpmI CTGGAG 1 cut(s) 690
BpuEI CTTGAG 1 cut(s) 635
BsaJI CCNNGG 5 cut(s) 19, 103, 191, 210, 972
BsaWI WCCGGW 2 cut(s) 28, 679
BsaXI ACNNNNNCTCC 2 cut(s) 420, 450
Bsc4I CCNNNNNNNGG 3 cut(s) 278, 449, 864
Bse1I ACTGG 2 cut(s) 312, 775
BseBI CCWGG 2 cut(s) 21, 756
BseDI CCNNGG 5 cut(s) 19, 103, 191, 210, 972
BseGI GGATG 2 cut(s) 201, 799
BseLI CCNNNNNNNGG 3 cut(s) 278, 449, 864
BseMII CTCAG 2 cut(s) 185, 626
BseNI ACTGG 2 cut(s) 312, 775
Bsh1236I CGCG 1 cut(s) 414
BshFI GGCC 2 cut(s) 5, 995
BsiHKAI GWGCWC 2 cut(s) 302, 969
BsiSI CCGG 3 cut(s) 29, 680, 816
BslI CCNNNNNNNGG 3 cut(s) 278, 449, 864
BsmAI GTCTC 4 cut(s) 233, 742, 924, 929
BsmBI CGTCTC 1 cut(s) 924
BsnI GGCC 2 cut(s) 5, 995
Bsp1286I GDGCHC 3 cut(s) 302, 969, 1138
Bsp1407I TGTACA 1 cut(s) 261
Bsp143I GATC 8 cut(s) 31, 76, 400, 493, 616, 987, 1036, 1103
BspACI CCGC 5 cut(s) 11, 100, 412, 1097, 1126
BspANI GGCC 2 cut(s) 5, 995
BspCNI CTCAG 2 cut(s) 184, 627
BspFNI CGCG 1 cut(s) 414
BspHI TCATGA 1 cut(s) 517
BspPI GGATC 3 cut(s) 39, 624, 995
BsrBI CCGCTC 1 cut(s) 1128
BsrGI TGTACA 1 cut(s) 261
BsrI ACTGG 2 cut(s) 312, 775
BssECI CCNNGG 5 cut(s) 19, 103, 191, 210, 972
BssMI GATC 8 cut(s) 31, 76, 400, 493, 616, 987, 1036, 1103
BssSI CACGAG 1 cut(s) 556
BssT1I CCWWGG 4 cut(s) 103, 191, 210, 972
Bst2BI CACGAG 1 cut(s) 556
Bst2UI CCWGG 2 cut(s) 21, 756
Bst4CI ACNGT 5 cut(s) 227, 632, 919, 964, 1087
Bst6I CTCTTC 3 cut(s) 240, 585, 1120
BstAUI TGTACA 1 cut(s) 261
BstC8I GCNNGC 2 cut(s) 326, 969
BstDEI CTNAG 3 cut(s) 171, 367, 635
BstF5I GGATG 2 cut(s) 201, 799
BstFNI CGCG 1 cut(s) 414
BstKTI GATC 8 cut(s) 34, 79, 403, 496, 619, 990, 1039, 1106
BstMAI GTCTC 4 cut(s) 233, 742, 924, 929
BstMBI GATC 8 cut(s) 31, 76, 400, 493, 616, 987, 1036, 1103
BstMWI GCNNNNNNNGC 1 cut(s) 334
BstNI CCWGG 2 cut(s) 21, 756
BstNSI RCATGY 3 cut(s) 462, 824, 982
BstSCI CCNGG 2 cut(s) 19, 754
BstUI CGCG 1 cut(s) 414
BstV2I GAAGAC 3 cut(s) 258, 369, 649
BstX2I RGATCY 1 cut(s) 76
BstYI RGATCY 1 cut(s) 76
BsuRI GGCC 2 cut(s) 5, 995
BtsCI GGATG 2 cut(s) 201, 799
BtsIMutI CAGTG 2 cut(s) 223, 969
Cac8I GCNNGC 2 cut(s) 326, 969
CciI TCATGA 1 cut(s) 517
Cfr13I GGNCC 3 cut(s) 23, 474, 772
Csp6I GTAC 1 cut(s) 262
CviAII CATG 9 cut(s) 82, 355, 459, 518, 604, 739, 821, 979, 1152
CviQI GTAC 1 cut(s) 262
DdeI CTNAG 3 cut(s) 171, 367, 635
DpnI GATC 8 cut(s) 33, 78, 402, 495, 618, 989, 1038, 1105
DpnII GATC 8 cut(s) 31, 76, 400, 493, 616, 987, 1036, 1103
DraI TTTAAA 1 cut(s) 1033
DrdI GACNNNNNNGTC 1 cut(s) 459
DseDI GACNNNNNNGTC 1 cut(s) 459
Eam1104I CTCTTC 3 cut(s) 240, 585, 1120
EarI CTCTTC 3 cut(s) 240, 585, 1120
Ecl136II GAGCTC 1 cut(s) 300
Eco130I CCWWGG 4 cut(s) 103, 191, 210, 972
Eco24I GRGCYC 2 cut(s) 302, 1138
Eco47I GGWCC 3 cut(s) 23, 474, 772
Eco53kI GAGCTC 1 cut(s) 300
Eco57I CTGAAG 3 cut(s) 57, 264, 1163
EcoICRI GAGCTC 1 cut(s) 300
EcoRI GAATTC 1 cut(s) 759
EcoRII CCWGG 2 cut(s) 19, 754
EcoT14I CCWWGG 4 cut(s) 103, 191, 210, 972
EcoT38I GRGCYC 2 cut(s) 302, 1138
ErhI CCWWGG 4 cut(s) 103, 191, 210, 972
Esp3I CGTCTC 1 cut(s) 924
FaeI CATG 9 cut(s) 85, 358, 462, 521, 607, 742, 824, 982, 1155
FatI CATG 9 cut(s) 81, 354, 458, 517, 603, 738, 820, 978, 1151
FauI CCCGC 2 cut(s) 419, 1133
FokI GGATG 2 cut(s) 208, 806
FriOI GRGCYC 2 cut(s) 302, 1138
FspBI CTAG 4 cut(s) 500, 699, 812, 1131
GsuI CTGGAG 1 cut(s) 690
HaeIII GGCC 2 cut(s) 5, 995
HapII CCGG 3 cut(s) 29, 680, 816
Hin1II CATG 9 cut(s) 85, 358, 462, 521, 607, 742, 824, 982, 1155
HindIII AAGCTT 1 cut(s) 65
HpaII CCGG 3 cut(s) 29, 680, 816
HphI GGTGA 1 cut(s) 488
Hpy166II GTNNAC 1 cut(s) 26
Hpy188I TCNGA 6 cut(s) 36, 76, 349, 465, 636, 849
Hpy188III TCNNGA 8 cut(s) 47, 162, 242, 398, 518, 541, 669, 1131
Hpy8I GTNNAC 1 cut(s) 26
HpyAV CCTTC 3 cut(s) 81, 763, 884
HpyCH4III ACNGT 5 cut(s) 227, 632, 919, 964, 1087
HpyCH4IV ACGT 1 cut(s) 124
HpyCH4V TGCA 4 cut(s) 85, 128, 337, 982
HpyF10VI GCNNNNNNNGC 1 cut(s) 334
HpyF3I CTNAG 3 cut(s) 171, 367, 635
HpySE526I ACGT 1 cut(s) 124
Hsp92II CATG 9 cut(s) 85, 358, 462, 521, 607, 742, 824, 982, 1155
Kzo9I GATC 8 cut(s) 31, 76, 400, 493, 616, 987, 1036, 1103
LmnI GCTCC 1 cut(s) 867
LweI GCATC 1 cut(s) 324
MaeI CTAG 4 cut(s) 500, 699, 812, 1131
MaeII ACGT 1 cut(s) 124
MaeIII GTNAC 1 cut(s) 805
MalI GATC 8 cut(s) 33, 78, 402, 495, 618, 989, 1038, 1105
MbiI CCGCTC 1 cut(s) 1128
MboI GATC 8 cut(s) 31, 76, 400, 493, 616, 987, 1036, 1103
MflI RGATCY 1 cut(s) 76
MhlI GDGCHC 3 cut(s) 302, 969, 1138
MluCI AATT 8 cut(s) 86, 166, 524, 640, 759, 828, 939, 997
MlyI GAGTC 2 cut(s) 447, 859
MmeI TCCRAC 3 cut(s) 145, 463, 1104
MnlI CCTC 5 cut(s) 16, 241, 565, 1082, 1123
MroXI GAANNNNTTC 1 cut(s) 785
MseI TTAA 5 cut(s) 612, 716, 827, 834, 1032
MslI CAYNNNNRTG 1 cut(s) 743
MspI CCGG 3 cut(s) 29, 680, 816
MspR9I CCNGG 2 cut(s) 21, 756
MvaI CCWGG 2 cut(s) 21, 756
MvnI CGCG 1 cut(s) 414
MwoI GCNNNNNNNGC 1 cut(s) 334
NdeII GATC 8 cut(s) 31, 76, 400, 493, 616, 987, 1036, 1103
NlaIII CATG 9 cut(s) 85, 358, 462, 521, 607, 742, 824, 982, 1155
NmeAIII GCCGAG 1 cut(s) 258
NmuCI GTSAC 1 cut(s) 805
NspI RCATGY 3 cut(s) 462, 824, 982
PagI TCATGA 1 cut(s) 517
PciI ACATGT 2 cut(s) 458, 820
PdmI GAANNNNTTC 1 cut(s) 785
PfeI GAWTC 9 cut(s) 199, 204, 344, 420, 433, 551, 781, 1076, 1166
PflMI CCANNNNNTGG 2 cut(s) 449, 864
PfoI TCCNGGA 1 cut(s) 754
PleI GAGTC 2 cut(s) 447, 858
PpsI GAGTC 2 cut(s) 447, 858
PscI ACATGT 2 cut(s) 458, 820
PsiI TTATAA 1 cut(s) 645
Psp124BI GAGCTC 1 cut(s) 302
Psp6I CCWGG 2 cut(s) 19, 754
PspGI CCWGG 2 cut(s) 19, 754
PspPI GGNCC 3 cut(s) 23, 474, 772
PsuI RGATCY 1 cut(s) 76
RsaI GTAC 1 cut(s) 263
RsaNI GTAC 1 cut(s) 262
RseI CAYNNNNRTG 1 cut(s) 743
SacI GAGCTC 1 cut(s) 302
SaqAI TTAA 5 cut(s) 612, 716, 827, 834, 1032
Sau3AI GATC 8 cut(s) 31, 76, 400, 493, 616, 987, 1036, 1103
Sau96I GGNCC 3 cut(s) 23, 474, 772
SchI GAGTC 2 cut(s) 447, 859
ScrFI CCNGG 2 cut(s) 21, 756
SduI GDGCHC 3 cut(s) 302, 969, 1138
SfaNI GCATC 1 cut(s) 324
SinI GGWCC 3 cut(s) 23, 474, 772
SmiMI CAYNNNNRTG 1 cut(s) 743
SmlI CTYRAG 1 cut(s) 650
SmoI CTYRAG 1 cut(s) 650
Sse9I AATT 8 cut(s) 86, 166, 524, 640, 759, 828, 939, 997
SsiI CCGC 5 cut(s) 11, 100, 412, 1097, 1126
SspMI CTAG 4 cut(s) 500, 699, 812, 1131
SstI GAGCTC 1 cut(s) 302
StyD4I CCNGG 2 cut(s) 19, 754
StyI CCWWGG 4 cut(s) 103, 191, 210, 972
TaaI ACNGT 5 cut(s) 227, 632, 919, 964, 1087
TaiI ACGT 1 cut(s) 127
TaqI TCGA 8 cut(s) 48, 62, 161, 202, 588, 660, 1068, 1074
TaqII GACCGA 1 cut(s) 491
TasI AATT 8 cut(s) 86, 166, 524, 640, 759, 828, 939, 997
TatI WGTACW 1 cut(s) 261
TfiI GAWTC 9 cut(s) 199, 204, 344, 420, 433, 551, 781, 1076, 1166
Tru1I TTAA 5 cut(s) 612, 716, 827, 834, 1032
Tru9I TTAA 5 cut(s) 612, 716, 827, 834, 1032
TscAI CASTG 2 cut(s) 230, 969
TseFI GTSAC 1 cut(s) 805
Tsp45I GTSAC 1 cut(s) 805
TspDTI ATGAA 4 cut(s) 132, 534, 782, 1051
TspGWI ACGGA 1 cut(s) 124
TspRI CASTG 2 cut(s) 230, 969
Van91I CCANNNNNTGG 2 cut(s) 449, 864
VpaK11BI GGWCC 3 cut(s) 23, 474, 772
XapI RAATTY 2 cut(s) 166, 759
XbaI TCTAGA 1 cut(s) 1130
XceI RCATGY 3 cut(s) 462, 824, 982
XcmI CCANNNNNNNNNTGG 1 cut(s) 446
XmnI GAANNNNTTC 1 cut(s) 785
XspI CTAG 4 cut(s) 500, 699, 812, 1131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.