AT1G27580

cysteine-rich repeat secretory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0000658
Physical Location & Seq
Reverse (-)
9589807 .. 9591075
1269 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G27580.1

Sequence Viewer

Length: 1095 bp
ATGCAAAGGTCTACTACGCTAACGCTTGACTCTGCTAAGGCTCTGCGTTCGAATTTGATTCAGCGGCCAAAAGCCAGATCTCCATTGCTGATGCTTTCTGCAGAAAAGGATGGTTGTCGTAGTAATAGTGTGTACAACCCATACAATGATAGGGTTTACGAGACGAAGAGTGACTTTTCAGGGTATCGATTCCTGGGAAACTCAGATGTATTTAGCGAGAAGAGGATCGATCTTCCACCACTAGAATCATTTAAGGGTAGCCATTTTAAGATTTGGCGTGAAGGAGAGGAGAATGTCAAGCATATGCTAATGGATGATGAGGATCCATTTCGTGATGAATTTCCTCATGCTGCCGAAGATCTGAGAGGTCGTTTGTGGGTTGACGACAAGAATGGAAACTACGTTGTTGTGTGGCAGTTTGAAATGTACCCATTTCTAGGGTTTTGCAGGACAGGGGATGATCATTACCGCGAGATTCCAACAGAGCTCGAGGTTCAGAGGGAGTTACGAGGTTTAAACGATATGGTACTGAAAGGTTACAATCTTTACGTCTATGCCACCCGTGAGTTCATTCGACACTTAGATTTGTCTGGACAAGATGAAGATGGTTTCAAGGATGTATCCGAGAAACATAGGCTTCCAATGTGGATGCCAAGTATGACAATGAAGGAACGTGAACGTATGAAGCAAAAGAGGTCTATCCCATCTAACAAAGGCATTGCTGTTACAAGAGCAGGTCAAGTTTTGGTTGTCTTTACGTACGATTTAGGTACCTCTGAAAGTCACAGGATGTTCCATCTGTACAAGAGGGATCCTAAAGATCTTGACCCGAAAACCCTCGACACCCGGCTTCTTGAGGTACATTCTCTAGGTGATGAGGCCCTCTTCTATGATTTGGGTATTACCGTGCCTGCTGACCATACCCTTGGAATTGAGCCTAACTCCATTTATTTCACTCGTGATGATCGTATCTGTCACAATAAATTGTCATGCCTCGACATCGGTGTCTTCAATCTTGCAACAAAGACCGTCAAACGCTTCCACGGTCTCTCCAACTTCAACTTCAACGATGCTCAGTGGTTTCTTCCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stress-antifung

Protein Analysis

364

Amino Acids

42.46

Weight (kDa)

6.2

Isoelectric Point (pI)

38.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 69 - 338 6e-31 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1004
Acc36I ACCTGC 1 cut(s) 725
Acc65I GGTACC 1 cut(s) 770
AccB1I GGYRCC 1 cut(s) 770
AccI GTMKAC 1 cut(s) 11
AccII CGCG 1 cut(s) 471
AciI CCGC 2 cut(s) 64, 469
AclWI GGATC 5 cut(s) 233, 317, 330, 806, 819
AcoI YGGCCR 1 cut(s) 65
AcsI RAATTY 2 cut(s) 52, 338
AfaI GTAC 7 cut(s) 134, 428, 528, 761, 772, 803, 861
AfiI CCNNNNNNNGG 1 cut(s) 437
AgsI TTSAA 5 cut(s) 422, 613, 1012, 1060, 1066
AjnI CCWGG 1 cut(s) 192
AjuI GAANNNNNNNTTGG 2 cut(s) 1046, 1078
AluBI AGCT 1 cut(s) 487
AluI AGCT 1 cut(s) 487
Alw21I GWGCWC 1 cut(s) 489
Alw26I GTCTC 2 cut(s) 155, 1052
AlwI GGATC 5 cut(s) 233, 317, 330, 806, 819
Ama87I CYCGRG 1 cut(s) 488
AoxI GGCC 2 cut(s) 65, 879
ApeKI GCWGC 1 cut(s) 350
ApoI RAATTY 2 cut(s) 52, 338
Asp718I GGTACC 1 cut(s) 770
AspS9I GGNCC 1 cut(s) 880
AsuC2I CCSGG 1 cut(s) 847
AsuHPI GGTGA 1 cut(s) 884
AsuII TTCGAA 1 cut(s) 50
AvaI CYCGRG 1 cut(s) 488
BamHI GGATCC 2 cut(s) 322, 811
BanI GGYRCC 1 cut(s) 770
BanII GRGCYC 1 cut(s) 489
BauI CACGAG 1 cut(s) 957
BbsI GAAGAC 1 cut(s) 1000
Bbv12I GWGCWC 1 cut(s) 489
BbvI GCAGC 1 cut(s) 337
BccI CCATC 4 cut(s) 104, 599, 712, 804
BciT130I CCWGG 1 cut(s) 194
BciVI GTATCC 1 cut(s) 631
BclI TGATCA 1 cut(s) 460
BcnI CCSGG 1 cut(s) 847
BcoDI GTCTC 2 cut(s) 155, 1052
BfaI CTAG 3 cut(s) 242, 437, 869
BfmI CTRYAG 1 cut(s) 99
BfuAI ACCTGC 1 cut(s) 725
BfuI GTATCC 1 cut(s) 631
BglII AGATCT 3 cut(s) 77, 358, 820
BisI GCNGC 2 cut(s) 65, 351
BlsI GCNGC 2 cut(s) 66, 352
Bme1390I CCNGG 2 cut(s) 194, 847
BmeT110I CYCGRG 1 cut(s) 488
BmgT120I GGNCC 1 cut(s) 880
BmiI GGNNCC 3 cut(s) 324, 772, 813
BmrFI CCNGG 2 cut(s) 194, 847
BmsI GCATC 3 cut(s) 81, 639, 1060
BpiI GAAGAC 1 cut(s) 1000
BplI GAGNNNNNCTC 2 cut(s) 926, 958
Bpu10I CCTNAGC 1 cut(s) 36
Bpu14I TTCGAA 1 cut(s) 50
BpuEI CTTGAG 1 cut(s) 875
BpuMI CCSGG 1 cut(s) 847
Bsa29I ATCGAT 2 cut(s) 187, 228
BsaAI YACGTR 1 cut(s) 759
BsaBI GATNNNNATC 1 cut(s) 321
BsaI GGTCTC 1 cut(s) 1052
BsaJI CCNNGG 3 cut(s) 193, 925, 1042
BsaXI ACNNNNNCTCC 2 cut(s) 1034, 1064
Bsc4I CCNNNNNNNGG 1 cut(s) 437
Bse3DI GCAATG 2 cut(s) 83, 717
Bse8I GATNNNNATC 1 cut(s) 321
BseBI CCWGG 1 cut(s) 194
BseCI ATCGAT 2 cut(s) 187, 228
BseDI CCNNGG 3 cut(s) 193, 925, 1042
BseGI GGATG 6 cut(s) 115, 319, 463, 622, 654, 795
BseJI GATNNNNATC 1 cut(s) 321
BseLI CCNNNNNNNGG 1 cut(s) 437
BseMI GCAATG 2 cut(s) 83, 717
BseMII CTCAG 3 cut(s) 216, 353, 1088
BseRI GAGGAG 1 cut(s) 302
BseXI GCAGC 1 cut(s) 337
Bsh1236I CGCG 1 cut(s) 471
BshFI GGCC 2 cut(s) 67, 881
BshNI GGYRCC 1 cut(s) 770
BshVI ATCGAT 2 cut(s) 187, 228
BsiHKAI GWGCWC 1 cut(s) 489
BsiHKCI CYCGRG 1 cut(s) 488
BsiSI CCGG 1 cut(s) 847
BsiWI CGTACG 1 cut(s) 759
BslI CCNNNNNNNGG 1 cut(s) 437
BsmAI GTCTC 2 cut(s) 155, 1052
BsmBI CGTCTC 1 cut(s) 155
BsnI GGCC 2 cut(s) 67, 881
Bso31I GGTCTC 1 cut(s) 1052
BsoBI CYCGRG 1 cut(s) 488
Bsp119I TTCGAA 1 cut(s) 50
Bsp1286I GDGCHC 1 cut(s) 489
Bsp1407I TGTACA 2 cut(s) 132, 801
Bsp143I GATC 9 cut(s) 77, 225, 229, 322, 358, 460, 811, 820, 964
BspACI CCGC 2 cut(s) 64, 469
BspANI GGCC 2 cut(s) 67, 881
BspCNI CTCAG 3 cut(s) 215, 354, 1087
BspDI ATCGAT 2 cut(s) 187, 228
BspFNI CGCG 1 cut(s) 471
BspLI GGNNCC 3 cut(s) 324, 772, 813
BspMAI CTGCAG 1 cut(s) 103
BspMI ACCTGC 1 cut(s) 725
BspPI GGATC 5 cut(s) 233, 317, 330, 806, 819
BspT104I TTCGAA 1 cut(s) 50
BspT107I GGYRCC 1 cut(s) 770
BspTNI GGTCTC 1 cut(s) 1052
BsrDI GCAATG 2 cut(s) 83, 717
BsrGI TGTACA 2 cut(s) 132, 801
BssECI CCNNGG 3 cut(s) 193, 925, 1042
BssMI GATC 9 cut(s) 77, 225, 229, 322, 358, 460, 811, 820, 964
BssSI CACGAG 1 cut(s) 957
BssT1I CCWWGG 1 cut(s) 925
Bst2BI CACGAG 1 cut(s) 957
Bst2UI CCWGG 1 cut(s) 194
Bst4CI ACNGT 3 cut(s) 907, 1030, 1046
Bst6I CTCTTC 3 cut(s) 161, 215, 890
BstAUI TGTACA 2 cut(s) 132, 801
BstBAI YACGTR 1 cut(s) 759
BstBI TTCGAA 1 cut(s) 50
BstC8I GCNNGC 1 cut(s) 912
BstDEI CTNAG 5 cut(s) 36, 202, 362, 580, 1074
BstDSI CCRYGG 1 cut(s) 1042
BstF5I GGATG 6 cut(s) 115, 319, 463, 622, 654, 795
BstFNI CGCG 1 cut(s) 471
BstKTI GATC 9 cut(s) 80, 228, 232, 325, 361, 463, 814, 823, 967
BstMAI GTCTC 2 cut(s) 155, 1052
BstMBI GATC 9 cut(s) 77, 225, 229, 322, 358, 460, 811, 820, 964
BstNI CCWGG 1 cut(s) 194
BstSCI CCNGG 2 cut(s) 192, 845
BstSFI CTRYAG 1 cut(s) 99
BstSNI TACGTA 1 cut(s) 759
BstUI CGCG 1 cut(s) 471
BstV1I GCAGC 1 cut(s) 337
BstV2I GAAGAC 1 cut(s) 1000
BstX2I RGATCY 5 cut(s) 77, 322, 358, 811, 820
BstXI CCANNNNNNTGG 1 cut(s) 926
BstYI RGATCY 5 cut(s) 77, 322, 358, 811, 820
Bsu15I ATCGAT 2 cut(s) 187, 228
BsuI GTATCC 1 cut(s) 631
BsuRI GGCC 2 cut(s) 67, 881
BsuTUI ATCGAT 2 cut(s) 187, 228
BtgI CCRYGG 1 cut(s) 1042
BtsCI GGATG 6 cut(s) 115, 319, 463, 622, 654, 795
BtsIMutI CAGTG 1 cut(s) 1082
BveI ACCTGC 1 cut(s) 725
Cac8I GCNNGC 1 cut(s) 912
Cfr13I GGNCC 1 cut(s) 880
ClaI ATCGAT 2 cut(s) 187, 228
Csp6I GTAC 7 cut(s) 133, 427, 527, 760, 771, 802, 860
CviAII CATG 2 cut(s) 347, 990
CviJI RGCY 9 cut(s) 41, 67, 74, 261, 487, 637, 850, 881, 937
CviKI_1 RGCY 9 cut(s) 41, 67, 74, 261, 487, 637, 850, 881, 937
CviQI GTAC 7 cut(s) 133, 427, 527, 760, 771, 802, 860
DdeI CTNAG 5 cut(s) 36, 202, 362, 580, 1074
DpnI GATC 9 cut(s) 79, 227, 231, 324, 360, 462, 813, 822, 966
DpnII GATC 9 cut(s) 77, 225, 229, 322, 358, 460, 811, 820, 964
DraI TTTAAA 1 cut(s) 516
DrdI GACNNNNNNGTC 1 cut(s) 1004
DseDI GACNNNNNNGTC 1 cut(s) 1004
EaeI YGGCCR 1 cut(s) 65
Eam1104I CTCTTC 3 cut(s) 161, 215, 890
EarI CTCTTC 3 cut(s) 161, 215, 890
Ecl136II GAGCTC 1 cut(s) 487
Eco105I TACGTA 1 cut(s) 759
Eco130I CCWWGG 1 cut(s) 925
Eco24I GRGCYC 1 cut(s) 489
Eco31I GGTCTC 1 cut(s) 1052
Eco53kI GAGCTC 1 cut(s) 487
Eco88I CYCGRG 1 cut(s) 488
EcoICRI GAGCTC 1 cut(s) 487
EcoO109I RGGNCCY 1 cut(s) 880
EcoRII CCWGG 1 cut(s) 192
EcoT14I CCWWGG 1 cut(s) 925
EcoT38I GRGCYC 1 cut(s) 489
ErhI CCWWGG 1 cut(s) 925
Esp3I CGTCTC 1 cut(s) 155
FaeI CATG 2 cut(s) 350, 993
FalI AAGNNNNNCTT 2 cut(s) 158, 190
FatI CATG 2 cut(s) 346, 989
FauNDI CATATG 1 cut(s) 303
FbaI TGATCA 1 cut(s) 460
FblI GTMKAC 1 cut(s) 11
Fnu4HI GCNGC 2 cut(s) 65, 351
FokI GGATG 6 cut(s) 122, 326, 470, 629, 661, 802
FriOI GRGCYC 1 cut(s) 489
Fsp4HI GCNGC 2 cut(s) 65, 351
FspBI CTAG 3 cut(s) 242, 437, 869
GluI GCNGC 2 cut(s) 65, 351
HaeIII GGCC 2 cut(s) 67, 881
HapII CCGG 1 cut(s) 847
Hin1II CATG 2 cut(s) 350, 993
HincII GTYRAC 1 cut(s) 382
HindII GTYRAC 1 cut(s) 382
HinfI GANTC 5 cut(s) 29, 58, 189, 245, 475
HpaII CCGG 1 cut(s) 847
HphI GGTGA 1 cut(s) 884
Hpy166II GTNNAC 5 cut(s) 12, 133, 157, 382, 677
Hpy188I TCNGA 5 cut(s) 205, 363, 498, 625, 778
Hpy188III TCNNGA 6 cut(s) 332, 591, 824, 854, 959, 1092
Hpy8I GTNNAC 5 cut(s) 12, 133, 157, 382, 677
HpyAV CCTTC 2 cut(s) 275, 661
HpyCH4III ACNGT 3 cut(s) 907, 1030, 1046
HpyCH4IV ACGT 5 cut(s) 402, 549, 673, 679, 758
HpyCH4V TGCA 4 cut(s) 4, 101, 447, 1019
HpyF3I CTNAG 5 cut(s) 36, 202, 362, 580, 1074
HpySE526I ACGT 5 cut(s) 402, 549, 673, 679, 758
Hsp92II CATG 2 cut(s) 350, 993
KpnI GGTACC 1 cut(s) 774
Ksp22I TGATCA 1 cut(s) 460
Kzo9I GATC 9 cut(s) 77, 225, 229, 322, 358, 460, 811, 820, 964
Lsp1109I GCAGC 1 cut(s) 337
LweI GCATC 3 cut(s) 81, 639, 1060
MaeI CTAG 3 cut(s) 242, 437, 869
MaeII ACGT 5 cut(s) 402, 549, 673, 679, 758
MaeIII GTNAC 6 cut(s) 170, 504, 536, 724, 782, 974
MalI GATC 9 cut(s) 79, 227, 231, 324, 360, 462, 813, 822, 966
MboI GATC 9 cut(s) 77, 225, 229, 322, 358, 460, 811, 820, 964
MboII GAAGA 8 cut(s) 178, 224, 232, 368, 614, 877, 1000, 1076
MflI RGATCY 5 cut(s) 77, 322, 358, 811, 820
MhlI GDGCHC 1 cut(s) 489
MluCI AATT 4 cut(s) 52, 338, 930, 983
MlyI GAGTC 1 cut(s) 23
MmeI TCCRAC 2 cut(s) 503, 1077
MseI TTAA 3 cut(s) 252, 267, 515
MspA1I CMGCKG 1 cut(s) 64
MspI CCGG 1 cut(s) 847
MspR9I CCNGG 2 cut(s) 194, 847
MssI GTTTAAAC 1 cut(s) 516
MvaI CCWGG 1 cut(s) 194
MvnI CGCG 1 cut(s) 471
NciI CCSGG 1 cut(s) 847
NdeI CATATG 1 cut(s) 303
NdeII GATC 9 cut(s) 77, 225, 229, 322, 358, 460, 811, 820, 964
NlaIII CATG 2 cut(s) 350, 993
NlaIV GGNNCC 3 cut(s) 324, 772, 813
NmuCI GTSAC 3 cut(s) 170, 782, 974
NspV TTCGAA 1 cut(s) 50
PaeR7I CTCGAG 1 cut(s) 488
PcsI WCGNNNNNNNCGW 1 cut(s) 964
PfeI GAWTC 4 cut(s) 58, 189, 245, 475
Pfl23II CGTACG 1 cut(s) 759
PkrI GCNGC 2 cut(s) 66, 352
PleI GAGTC 1 cut(s) 23
PmeI GTTTAAAC 1 cut(s) 516
PpsI GAGTC 1 cut(s) 23
Ppu21I YACGTR 1 cut(s) 759
Psp124BI GAGCTC 1 cut(s) 489
Psp6I CCWGG 1 cut(s) 192
PspGI CCWGG 1 cut(s) 192
PspLI CGTACG 1 cut(s) 759
PspN4I GGNNCC 3 cut(s) 324, 772, 813
PspPI GGNCC 1 cut(s) 880
PspXI VCTCGAGB 1 cut(s) 488
PstI CTGCAG 1 cut(s) 103
PsuI RGATCY 5 cut(s) 77, 322, 358, 811, 820
RsaI GTAC 7 cut(s) 134, 428, 528, 761, 772, 803, 861
RsaNI GTAC 7 cut(s) 133, 427, 527, 760, 771, 802, 860
SacI GAGCTC 1 cut(s) 489
SaqAI TTAA 3 cut(s) 252, 267, 515
SatI GCNGC 2 cut(s) 65, 351
Sau3AI GATC 9 cut(s) 77, 225, 229, 322, 358, 460, 811, 820, 964
Sau96I GGNCC 1 cut(s) 880
SchI GAGTC 1 cut(s) 23
ScrFI CCNGG 2 cut(s) 194, 847
SduI GDGCHC 1 cut(s) 489
SfaNI GCATC 3 cut(s) 81, 639, 1060
SfcI CTRYAG 1 cut(s) 99
Sfr274I CTCGAG 1 cut(s) 488
SfuI TTCGAA 1 cut(s) 50
SlaI CTCGAG 1 cut(s) 488
SmlI CTYRAG 2 cut(s) 488, 854
SmoI CTYRAG 2 cut(s) 488, 854
SnaBI TACGTA 1 cut(s) 759
Sse9I AATT 4 cut(s) 52, 338, 930, 983
SsiI CCGC 2 cut(s) 64, 469
SspMI CTAG 3 cut(s) 242, 437, 869
SstI GAGCTC 1 cut(s) 489
StyD4I CCNGG 2 cut(s) 192, 845
StyI CCWWGG 1 cut(s) 925
TaaI ACNGT 3 cut(s) 907, 1030, 1046
TaiI ACGT 5 cut(s) 405, 552, 676, 682, 761
TaqI TCGA 7 cut(s) 50, 187, 228, 489, 574, 840, 996
TasI AATT 4 cut(s) 52, 338, 930, 983
TatI WGTACW 2 cut(s) 132, 801
TauI GCSGC 1 cut(s) 67
TfiI GAWTC 4 cut(s) 58, 189, 245, 475
Tru1I TTAA 3 cut(s) 252, 267, 515
Tru9I TTAA 3 cut(s) 252, 267, 515
TscAI CASTG 1 cut(s) 1082
TseFI GTSAC 3 cut(s) 170, 782, 974
TseI GCWGC 1 cut(s) 350
Tsp45I GTSAC 3 cut(s) 170, 782, 974
TspDTI ATGAA 5 cut(s) 351, 559, 615, 680, 698
TspRI CASTG 1 cut(s) 1082
XapI RAATTY 2 cut(s) 52, 338
XhoI CTCGAG 1 cut(s) 488
XmiI GTMKAC 1 cut(s) 11
XspI CTAG 3 cut(s) 242, 437, 869
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.