AT4G22165

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0000658
Physical Location & Seq
Forward (+)
11734328 .. 11735419
1092 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G22165.1

Sequence Viewer

Length: 1092 bp
ATGGAGAAGAACCATAACCCTAATACATGGTCGGAGCTTCCTCTAGATCTCTTGAACTTAGTTTTCAAACGCCTCAGCTTAGTTAATTTCCAACGAGCTAAATCCGTATGCTCCACTCGGTACTCTGTTTCGAGACAATGCGTGCCCGAACGTCAGATCGCTTTGCTGATTCTCTTCCCCAAAGAAGACAACACCGATAACAGTACTTGTAAGTTGTTCAATCCCGACGAAAAAGACAAACTTTACAAGATGCAAGATCTTGGTGTGGAATTTGCAAAGAGTGTTTGTAGGGCAACTTATGGAAGTTGGCTCCTGATGCAAGATTCAAAGTATCATCTATACATTTTGAATATATTCACTCGCAAGAGGATCAATCTTCCTCCTGTGGAGTCACAACTTGGCATGGTAAAGATTGAACGAACCATATATGATTGGTTTCACTTTTCACACGGTCACTATAGCTTTTCGCTAAGTTCCCCTGTGTTTTGGATTGACGAGGAATCCAAAGATTATATAGTTATGTGGGGACTTGGAGTTTATTGTGTGGTTTATGCCAAGAAAGGAGATACCTCGTGGAATCAAATCCCCCAAACTTCATATTTTTATGACATGGTTTACAAGGATCACAAGCTTTACTTCTTAAGCAGTACTGGTACTTTCCAAATCTTAGATTTTTCTGAAGAGATGGACAACAAAACTTCCAAGGTGGTATGCTTGTTAGATAGAAAACTTGTAGTCACGGTAACAGGAAAAGCCCTCAAGGTTGCAAAAATGTGGAGACCTACATATAGAACATGGTCCTTTCGGGTTTTCAAGATTTCTTCATCAGGATATGAAAAACTTGATTCTTTGGGTGATGAGGCATTGCTTTTGGATCTAGGCATCACTGTGCTCGCCAGTGACGTTGAGGGATTCAAAAGAAATTCCATCTATTTCAGTTGTAGACCTAGTGGTGGTTATGAAACTAACGCAAGTGATTTATTTCTTTTCAATCTCGAGACACAGAAGATGGAGCTACTGCACAAATTTGATTGTTCGTCCCTTCAACTCTATAGATCTCGATGGTTCTTACCAAGTTTGACTCACACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box

Protein Analysis

363

Amino Acids

42.38

Weight (kDa)

8.6

Isoelectric Point (pI)

39.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 73 - 330 8.8e-51 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 943
AclWI GGATC 3 cut(s) 377, 630, 882
AcsI RAATTY 3 cut(s) 269, 922, 1025
AcuI CTGAAG 1 cut(s) 699
AfaI GTAC 4 cut(s) 122, 205, 649, 655
AfiI CCNNNNNNNGG 1 cut(s) 953
AflII CTTAAG 1 cut(s) 640
AluBI AGCT 6 cut(s) 37, 78, 98, 462, 631, 1015
AluI AGCT 6 cut(s) 37, 78, 98, 462, 631, 1015
Alw21I GWGCWC 1 cut(s) 894
Alw26I GTCTC 3 cut(s) 127, 772, 992
AlwI GGATC 3 cut(s) 377, 630, 882
Ama87I CYCGRG 1 cut(s) 995
ApoI RAATTY 3 cut(s) 269, 922, 1025
Asp700I GAANNNNTTC 1 cut(s) 353
AspS9I GGNCC 1 cut(s) 798
AsuHPI GGTGA 1 cut(s) 866
AvaI CYCGRG 1 cut(s) 995
AvaII GGWCC 1 cut(s) 798
BaeGI GKGCMC 1 cut(s) 147
BauI CACGAG 1 cut(s) 571
BbsI GAAGAC 1 cut(s) 192
Bbv12I GWGCWC 1 cut(s) 894
BbvCI CCTCAGC 1 cut(s) 74
BccI CCATC 4 cut(s) 679, 935, 1003, 1056
BcoDI GTCTC 3 cut(s) 127, 772, 992
BfaI CTAG 3 cut(s) 44, 878, 948
BfmI CTRYAG 2 cut(s) 457, 1051
BfrI CTTAAG 1 cut(s) 640
BglII AGATCT 3 cut(s) 46, 256, 1055
BmcAI AGTACT 2 cut(s) 205, 649
Bme18I GGWCC 1 cut(s) 798
BmeT110I CYCGRG 1 cut(s) 995
BmgT120I GGNCC 1 cut(s) 798
BmiI GGNNCC 1 cut(s) 311
BmsI GCATC 3 cut(s) 240, 306, 891
BpiI GAAGAC 1 cut(s) 192
Bpu10I CCTNAGC 1 cut(s) 74
BpuEI CTTGAG 1 cut(s) 743
BsaI GGTCTC 1 cut(s) 772
BsaJI CCNNGG 1 cut(s) 702
Bsc4I CCNNNNNNNGG 1 cut(s) 953
Bse1I ACTGG 2 cut(s) 655, 897
Bse3DI GCAATG 1 cut(s) 863
BseDI CCNNGG 1 cut(s) 702
BseLI CCNNNNNNNGG 1 cut(s) 953
BseMI GCAATG 1 cut(s) 863
BseMII CTCAG 1 cut(s) 88
BseNI ACTGG 2 cut(s) 655, 897
BseSI GKGCMC 1 cut(s) 147
BsgI GTGCAG 1 cut(s) 1004
BsiHKAI GWGCWC 1 cut(s) 894
BsiHKCI CYCGRG 1 cut(s) 995
BslFI GGGAC 2 cut(s) 540, 1024
BslI CCNNNNNNNGG 1 cut(s) 953
BsmAI GTCTC 3 cut(s) 127, 772, 992
BsmFI GGGAC 2 cut(s) 540, 1024
Bso31I GGTCTC 1 cut(s) 772
BsoBI CYCGRG 1 cut(s) 995
Bsp1286I GDGCHC 2 cut(s) 147, 894
Bsp143I GATC 7 cut(s) 46, 156, 256, 369, 622, 874, 1055
BspCNI CTCAG 1 cut(s) 87
BspLI GGNNCC 1 cut(s) 311
BspPI GGATC 3 cut(s) 377, 630, 882
BspTI CTTAAG 1 cut(s) 640
BspTNI GGTCTC 1 cut(s) 772
BsrDI GCAATG 1 cut(s) 863
BsrI ACTGG 2 cut(s) 655, 897
BssECI CCNNGG 1 cut(s) 702
BssMI GATC 7 cut(s) 46, 156, 256, 369, 622, 874, 1055
BssSI CACGAG 1 cut(s) 571
BssT1I CCWWGG 1 cut(s) 702
Bst2BI CACGAG 1 cut(s) 571
Bst4CI ACNGT 4 cut(s) 203, 452, 742, 889
Bst6I CTCTTC 2 cut(s) 179, 675
BstAFI CTTAAG 1 cut(s) 640
BstC8I GCNNGC 2 cut(s) 143, 894
BstDEI CTNAG 5 cut(s) 58, 74, 79, 470, 667
BstKTI GATC 7 cut(s) 49, 159, 259, 372, 625, 877, 1058
BstMAI GTCTC 3 cut(s) 127, 772, 992
BstMBI GATC 7 cut(s) 46, 156, 256, 369, 622, 874, 1055
BstMWI GCNNNNNNNGC 1 cut(s) 316
BstSFI CTRYAG 2 cut(s) 457, 1051
BstSLI GKGCMC 1 cut(s) 147
BstV2I GAAGAC 1 cut(s) 192
BstX2I RGATCY 4 cut(s) 46, 256, 874, 1055
BstYI RGATCY 4 cut(s) 46, 256, 874, 1055
BtsIMutI CAGTG 2 cut(s) 885, 904
Cac8I GCNNGC 2 cut(s) 143, 894
Cfr13I GGNCC 1 cut(s) 798
Csp6I GTAC 4 cut(s) 121, 204, 648, 654
CviAII CATG 5 cut(s) 27, 403, 610, 795, 1089
CviJI RGCY 8 cut(s) 37, 78, 98, 310, 462, 631, 755, 1015
CviKI_1 RGCY 8 cut(s) 37, 78, 98, 310, 462, 631, 755, 1015
CviQI GTAC 4 cut(s) 121, 204, 648, 654
DdeI CTNAG 5 cut(s) 58, 74, 79, 470, 667
DpnI GATC 7 cut(s) 48, 158, 258, 371, 624, 876, 1057
DpnII GATC 7 cut(s) 46, 156, 256, 369, 622, 874, 1055
Eam1104I CTCTTC 2 cut(s) 179, 675
EarI CTCTTC 2 cut(s) 179, 675
Eco130I CCWWGG 1 cut(s) 702
Eco31I GGTCTC 1 cut(s) 772
Eco47I GGWCC 1 cut(s) 798
Eco57I CTGAAG 1 cut(s) 699
Eco88I CYCGRG 1 cut(s) 995
EcoT14I CCWWGG 1 cut(s) 702
ErhI CCWWGG 1 cut(s) 702
FaeI CATG 5 cut(s) 30, 406, 613, 798, 1092
FalI AAGNNNNNCTT 4 cut(s) 225, 257, 620, 652
FaqI GGGAC 2 cut(s) 540, 1024
FatI CATG 5 cut(s) 26, 402, 609, 794, 1088
FblI GTMKAC 1 cut(s) 943
FspBI CTAG 3 cut(s) 44, 878, 948
Hin1II CATG 5 cut(s) 30, 406, 613, 798, 1092
HindIII AAGCTT 1 cut(s) 629
HinfI GANTC 8 cut(s) 169, 323, 389, 500, 577, 845, 912, 1081
HphI GGTGA 1 cut(s) 866
Hpy166II GTNNAC 2 cut(s) 616, 944
Hpy188I TCNGA 3 cut(s) 34, 156, 679
Hpy8I GTNNAC 2 cut(s) 616, 944
Hpy99I CGWCG 1 cut(s) 230
HpyAV CCTTC 1 cut(s) 1052
HpyCH4III ACNGT 4 cut(s) 203, 452, 742, 889
HpyCH4IV ACGT 2 cut(s) 151, 903
HpyCH4V TGCA 5 cut(s) 253, 275, 319, 767, 1021
HpyF10VI GCNNNNNNNGC 1 cut(s) 316
HpyF3I CTNAG 5 cut(s) 58, 74, 79, 470, 667
HpySE526I ACGT 2 cut(s) 151, 903
Hsp92II CATG 5 cut(s) 30, 406, 613, 798, 1092
Kzo9I GATC 7 cut(s) 46, 156, 256, 369, 622, 874, 1055
LmnI GCTCC 4 cut(s) 34, 116, 315, 1012
LpnPI CCDG 7 cut(s) 326, 396, 492, 636, 732, 813, 910
LweI GCATC 3 cut(s) 240, 306, 891
MaeI CTAG 3 cut(s) 44, 878, 948
MaeII ACGT 2 cut(s) 151, 903
MaeIII GTNAC 5 cut(s) 390, 452, 736, 742, 899
MalI GATC 7 cut(s) 48, 158, 258, 371, 624, 876, 1057
MboI GATC 7 cut(s) 46, 156, 256, 369, 622, 874, 1055
MboII GAAGA 7 cut(s) 19, 166, 197, 368, 692, 813, 1018
MflI RGATCY 4 cut(s) 46, 256, 874, 1055
MhlI GDGCHC 2 cut(s) 147, 894
MluCI AATT 4 cut(s) 85, 269, 922, 1025
MlyI GAGTC 2 cut(s) 398, 1075
MmeI TCCRAC 2 cut(s) 12, 115
MnlI CCTC 9 cut(s) 51, 83, 360, 390, 490, 580, 767, 853, 901
MroXI GAANNNNTTC 1 cut(s) 353
MseI TTAA 2 cut(s) 84, 641
MslI CAYNNNNRTG 1 cut(s) 887
MspCI CTTAAG 1 cut(s) 640
MwoI GCNNNNNNNGC 1 cut(s) 316
NdeII GATC 7 cut(s) 46, 156, 256, 369, 622, 874, 1055
NlaIII CATG 5 cut(s) 30, 406, 613, 798, 1092
NlaIV GGNNCC 1 cut(s) 311
NmuCI GTSAC 4 cut(s) 390, 452, 736, 899
PaeR7I CTCGAG 1 cut(s) 995
PcsI WCGNNNNNNNCGW 1 cut(s) 900
PdmI GAANNNNTTC 1 cut(s) 353
PfeI GAWTC 6 cut(s) 169, 323, 500, 577, 845, 912
PleI GAGTC 2 cut(s) 397, 1075
PpsI GAGTC 2 cut(s) 397, 1075
PspN4I GGNNCC 1 cut(s) 311
PspPI GGNCC 1 cut(s) 798
PsuI RGATCY 4 cut(s) 46, 256, 874, 1055
RsaI GTAC 4 cut(s) 122, 205, 649, 655
RsaNI GTAC 4 cut(s) 121, 204, 648, 654
RseI CAYNNNNRTG 1 cut(s) 887
SaqAI TTAA 2 cut(s) 84, 641
Sau3AI GATC 7 cut(s) 46, 156, 256, 369, 622, 874, 1055
Sau96I GGNCC 1 cut(s) 798
ScaI AGTACT 2 cut(s) 205, 649
SchI GAGTC 2 cut(s) 398, 1075
SduI GDGCHC 2 cut(s) 147, 894
SfaNI GCATC 3 cut(s) 240, 306, 891
SfcI CTRYAG 2 cut(s) 457, 1051
Sfr274I CTCGAG 1 cut(s) 995
SinI GGWCC 1 cut(s) 798
SlaI CTCGAG 1 cut(s) 995
SmiMI CAYNNNNRTG 1 cut(s) 887
SmlI CTYRAG 3 cut(s) 640, 758, 995
SmoI CTYRAG 3 cut(s) 640, 758, 995
Sse9I AATT 4 cut(s) 85, 269, 922, 1025
SspMI CTAG 3 cut(s) 44, 878, 948
StyI CCWWGG 1 cut(s) 702
TaaI ACNGT 4 cut(s) 203, 452, 742, 889
TaiI ACGT 2 cut(s) 154, 906
TaqI TCGA 3 cut(s) 131, 996, 1060
TasI AATT 4 cut(s) 85, 269, 922, 1025
TatI WGTACW 2 cut(s) 203, 647
TfiI GAWTC 6 cut(s) 169, 323, 500, 577, 845, 912
Tru1I TTAA 2 cut(s) 84, 641
Tru9I TTAA 2 cut(s) 84, 641
TscAI CASTG 2 cut(s) 892, 904
TseFI GTSAC 4 cut(s) 390, 452, 736, 899
Tsp45I GTSAC 4 cut(s) 390, 452, 736, 899
TspDTI ATGAA 4 cut(s) 585, 813, 849, 975
TspGWI ACGGA 1 cut(s) 94
TspRI CASTG 2 cut(s) 892, 904
Vha464I CTTAAG 1 cut(s) 640
VpaK11BI GGWCC 1 cut(s) 798
XapI RAATTY 3 cut(s) 269, 922, 1025
XbaI TCTAGA 1 cut(s) 43
XhoI CTCGAG 1 cut(s) 995
XmiI GTMKAC 1 cut(s) 943
XmnI GAANNNNTTC 1 cut(s) 353
XspI CTAG 3 cut(s) 44, 878, 948
ZrmI AGTACT 2 cut(s) 205, 649
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.