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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 2 cut(s) | 144, 255 |
| AciI | CCGC | 2 cut(s) | 137, 186 |
| AcoI | YGGCCR | 2 cut(s) | 72, 138 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 221 |
| AjiI | CACGTC | 1 cut(s) | 220 |
| AluBI | AGCT | 2 cut(s) | 299, 303 |
| AluI | AGCT | 2 cut(s) | 299, 303 |
| Alw26I | GTCTC | 3 cut(s) | 55, 118, 143 |
| AoxI | GGCC | 3 cut(s) | 72, 138, 212 |
| AsuII | TTCGAA | 1 cut(s) | 239 |
| AsuNHI | GCTAGC | 1 cut(s) | 299 |
| BceAI | ACGGC | 3 cut(s) | 87, 125, 150 |
| BcoDI | GTCTC | 3 cut(s) | 55, 118, 143 |
| BfaI | CTAG | 2 cut(s) | 300, 304 |
| BisI | GCNGC | 1 cut(s) | 138 |
| BlsI | GCNGC | 1 cut(s) | 139 |
| BmgBI | CACGTC | 1 cut(s) | 220 |
| BmtI | GCTAGC | 1 cut(s) | 303 |
| Bpu14I | TTCGAA | 1 cut(s) | 239 |
| BsaI | GGTCTC | 2 cut(s) | 55, 118 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 221 |
| BseGI | GGATG | 1 cut(s) | 190 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 221 |
| BseX3I | CGGCCG | 1 cut(s) | 138 |
| Bsh1285I | CGRYCG | 1 cut(s) | 141 |
| BshFI | GGCC | 3 cut(s) | 74, 140, 214 |
| BsiEI | CGRYCG | 1 cut(s) | 141 |
| BsiSI | CCGG | 1 cut(s) | 183 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 221 |
| BsmAI | GTCTC | 3 cut(s) | 55, 118, 143 |
| BsmBI | CGTCTC | 1 cut(s) | 143 |
| BsnI | GGCC | 3 cut(s) | 74, 140, 214 |
| Bso31I | GGTCTC | 2 cut(s) | 55, 118 |
| Bsp119I | TTCGAA | 1 cut(s) | 239 |
| Bsp143I | GATC | 1 cut(s) | 16 |
| BspACI | CCGC | 2 cut(s) | 137, 186 |
| BspANI | GGCC | 3 cut(s) | 74, 140, 214 |
| BspOI | GCTAGC | 1 cut(s) | 303 |
| BspT104I | TTCGAA | 1 cut(s) | 239 |
| BspTNI | GGTCTC | 2 cut(s) | 55, 118 |
| BssMI | GATC | 1 cut(s) | 16 |
| Bst6I | CTCTTC | 1 cut(s) | 116 |
| BstBI | TTCGAA | 1 cut(s) | 239 |
| BstC8I | GCNNGC | 2 cut(s) | 12, 301 |
| BstF5I | GGATG | 1 cut(s) | 190 |
| BstKTI | GATC | 1 cut(s) | 19 |
| BstMAI | GTCTC | 3 cut(s) | 55, 118, 143 |
| BstMBI | GATC | 1 cut(s) | 16 |
| BstMCI | CGRYCG | 1 cut(s) | 141 |
| BstZI | CGGCCG | 1 cut(s) | 138 |
| BsuRI | GGCC | 3 cut(s) | 74, 140, 214 |
| BtrI | CACGTC | 1 cut(s) | 220 |
| BtsCI | GGATG | 1 cut(s) | 190 |
| Cac8I | GCNNGC | 2 cut(s) | 12, 301 |
| CviJI | RGCY | 5 cut(s) | 74, 140, 214, 299, 303 |
| CviKI_1 | RGCY | 5 cut(s) | 74, 140, 214, 299, 303 |
| DpnI | GATC | 1 cut(s) | 18 |
| DpnII | GATC | 1 cut(s) | 16 |
| EaeI | YGGCCR | 2 cut(s) | 72, 138 |
| EagI | CGGCCG | 1 cut(s) | 138 |
| Eam1104I | CTCTTC | 1 cut(s) | 116 |
| EarI | CTCTTC | 1 cut(s) | 116 |
| EclXI | CGGCCG | 1 cut(s) | 138 |
| Eco147I | AGGCCT | 1 cut(s) | 214 |
| Eco31I | GGTCTC | 2 cut(s) | 55, 118 |
| Eco52I | CGGCCG | 1 cut(s) | 138 |
| Esp3I | CGTCTC | 1 cut(s) | 143 |
| FaiI | YATR | 3 cut(s) | 21, 84, 278 |
| FblI | GTMKAC | 2 cut(s) | 144, 255 |
| Fnu4HI | GCNGC | 1 cut(s) | 138 |
| FokI | GGATG | 1 cut(s) | 177 |
| Fsp4HI | GCNGC | 1 cut(s) | 138 |
| FspBI | CTAG | 2 cut(s) | 300, 304 |
| GluI | GCNGC | 1 cut(s) | 138 |
| HaeIII | GGCC | 3 cut(s) | 74, 140, 214 |
| HapII | CCGG | 1 cut(s) | 183 |
| HincII | GTYRAC | 3 cut(s) | 145, 256, 291 |
| HindII | GTYRAC | 3 cut(s) | 145, 256, 291 |
| HpaII | CCGG | 1 cut(s) | 183 |
| Hpy166II | GTNNAC | 4 cut(s) | 55, 145, 256, 291 |
| Hpy8I | GTNNAC | 4 cut(s) | 55, 145, 256, 291 |
| Hpy99I | CGWCG | 2 cut(s) | 146, 149 |
| HpyAV | CCTTC | 4 cut(s) | 35, 98, 204, 244 |
| HpyCH4IV | ACGT | 2 cut(s) | 152, 219 |
| HpyCH4V | TGCA | 1 cut(s) | 10 |
| HpySE526I | ACGT | 2 cut(s) | 152, 219 |
| Kzo9I | GATC | 1 cut(s) | 16 |
| LpnPI | CCDG | 4 cut(s) | 43, 196, 214, 228 |
| MaeI | CTAG | 2 cut(s) | 300, 304 |
| MaeII | ACGT | 2 cut(s) | 152, 219 |
| MalI | GATC | 1 cut(s) | 18 |
| MboI | GATC | 1 cut(s) | 16 |
| MboII | GAAGA | 3 cut(s) | 121, 133, 253 |
| MnlI | CCTC | 4 cut(s) | 24, 87, 190, 204 |
| MspI | CCGG | 1 cut(s) | 183 |
| NdeII | GATC | 1 cut(s) | 16 |
| NheI | GCTAGC | 1 cut(s) | 299 |
| NspV | TTCGAA | 1 cut(s) | 239 |
| PceI | AGGCCT | 1 cut(s) | 214 |
| PkrI | GCNGC | 1 cut(s) | 139 |
| SalI | GTCGAC | 2 cut(s) | 143, 254 |
| SatI | GCNGC | 1 cut(s) | 138 |
| Sau3AI | GATC | 1 cut(s) | 16 |
| SetI | ASST | 7 cut(s) | 155, 222, 255, 261, 290, 301, 305 |
| SfuI | TTCGAA | 1 cut(s) | 239 |
| SgrDI | CGTCGACG | 1 cut(s) | 143 |
| SseBI | AGGCCT | 1 cut(s) | 214 |
| SsiI | CCGC | 2 cut(s) | 137, 186 |
| SspMI | CTAG | 2 cut(s) | 300, 304 |
| StuI | AGGCCT | 1 cut(s) | 214 |
| TaiI | ACGT | 2 cut(s) | 155, 222 |
| TaqI | TCGA | 4 cut(s) | 144, 196, 239, 255 |
| TauI | GCSGC | 1 cut(s) | 140 |
| TspDTI | ATGAA | 1 cut(s) | 54 |
| XmiI | GTMKAC | 2 cut(s) | 144, 255 |
| XspI | CTAG | 2 cut(s) | 300, 304 |