AT4G22060

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0000658
Physical Location & Seq
Forward (+)
11687595 .. 11689021
1427 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G22060.1

Sequence Viewer

Length: 1200 bp
ATGGATCCCCCTTCTCTGATGTCTGATGGCTCAAGCTGGTCCAAGCTTCCTTTAGATCTCTTGATCATGGTTTTCGAACGTCTTGGTTTTGTAGATTTTCAACGAACTAAATCAGTGTGTCTAGCTTGGCTATATGCTTCACGAATGTCAGCGCCAAACAAGCAGATCCCTTGGCTCATTATGTTTCCAGAAAAAGGTAAAGATTTTTGTCTCTTGTTTAATTCTGAAGAAAAAGAGAAGATATATAGGATTCAAAATCTTGGTGTTGAATTCGCAAATAGTCACTGTTTGGCGATTTATGGAAGTTGGCTCTTTATGAGAGATCCTAGATATAAGCTCTATATAATGAATTTGTTTACTCGTGAGAGAATTAATCTTCCGTCTGTGGAGTCTCAATTTGGTAGGATAAAGATTGAACAAATTAACGATGATTTGTTCTATAGAAAAGTTGATGATGAATATGATTACCATCCTAAAAGACACATGATCGATATTAGTGACCATATATTATGGATAGATGATAAGACCAAAGATTATGTGGTTATGTGGTCATTCGAGTGCGGATACACATATATGGTTTATTGCAGAACTGGAGATAATATTTGGAATTACCGCAGTCTTGATATATCGACTGTTAACATAGTATACAAAGATCATAAGATGTACTTGTATAGTTACACTCGTGATGTCAAAGTCTTGGATTTTTGTGAAGATATTCCGCGACAAATCTTTGAAACGCAAGTTAATTATGATATATTAATGGAAAAAGGATTCAGAAGTGACGTTGATGATGTGTTGAATGATAAAAAGGAACATCTTGTAGTCACATTAAATGGAGATGTCCTTAGAGTTAAGAGCAAAATATGGGATAATAGTGACGTTTGGTCCTTCTGCATCTACAAGTTGAATTCTTCTAATACTTACTGGGAGAAACTCACTTCTTTGGGAGACGAGGCAATACTTTTGGATCTAGGTATCACTGTACTTGCCAACACCATCGAAGGAATCAATAGAAATTCAATATATTTCAGTGGTTATCATCGCCCTCATTATTTCAGATTCGATCATGTTTGGAGTGAAAAAGATATTTGTGTCTTCAATCTTGACACACAAGAAGTTGAAAGACCACATCAAAGTATTTGTTCGTCCATTCAAATATCTGGTGCACGATGGTTTGTGCCAAATTTCAAACATATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box

Protein Analysis

399

Amino Acids

47.47

Weight (kDa)

5.71

Isoelectric Point (pI)

38.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 73 - 367 6.4e-51 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 645
AccII CGCG 1 cut(s) 721
AciI CCGC 3 cut(s) 561, 613, 719
AclWI GGATC 4 cut(s) 12, 160, 317, 975
AcsI RAATTY 5 cut(s) 269, 349, 907, 1015, 1183
AcuI CTGAAG 1 cut(s) 246
AfaI GTAC 2 cut(s) 665, 984
AfiI CCNNNNNNNGG 1 cut(s) 194
AhdI GACNNNNNGTC 1 cut(s) 883
AluBI AGCT 4 cut(s) 36, 46, 125, 337
AluI AGCT 4 cut(s) 36, 46, 125, 337
Alw21I GWGCWC 1 cut(s) 1168
Alw26I GTCTC 3 cut(s) 215, 396, 942
Alw44I GTGCAC 1 cut(s) 1164
AlwI GGATC 4 cut(s) 12, 160, 317, 975
ApaLI GTGCAC 1 cut(s) 1164
ApoI RAATTY 5 cut(s) 269, 349, 907, 1015, 1183
AseI ATTAAT 2 cut(s) 372, 758
Asp700I GAANNNNTTC 1 cut(s) 714
AspLEI GCGC 1 cut(s) 154
AspS9I GGNCC 2 cut(s) 39, 885
AsuII TTCGAA 1 cut(s) 75
AvaII GGWCC 2 cut(s) 39, 885
BaeGI GKGCMC 1 cut(s) 1168
BamHI GGATCC 1 cut(s) 4
BauI CACGAG 2 cut(s) 360, 681
BbsI GAAGAC 1 cut(s) 1087
Bbv12I GWGCWC 1 cut(s) 1168
BccI CCATC 4 cut(s) 20, 477, 1004, 1164
BciVI GTATCC 1 cut(s) 557
BclI TGATCA 1 cut(s) 63
BcoDI GTCTC 3 cut(s) 215, 396, 942
BfaI CTAG 3 cut(s) 122, 327, 971
BfmI CTRYAG 1 cut(s) 439
BfoI RGCGCY 1 cut(s) 155
BfuI GTATCC 1 cut(s) 557
BglII AGATCT 1 cut(s) 55
Bme18I GGWCC 2 cut(s) 39, 885
BmeRI GACNNNNNGTC 1 cut(s) 883
BmgT120I GGNCC 2 cut(s) 39, 885
BmiI GGNNCC 1 cut(s) 6
BmrI ACTGGG 1 cut(s) 934
BmsI GCATC 1 cut(s) 903
BmuI ACTGGG 1 cut(s) 934
BpiI GAAGAC 1 cut(s) 1087
BpmI CTGGAG 1 cut(s) 612
Bpu14I TTCGAA 1 cut(s) 75
BpuEI CTTGAG 1 cut(s) 16
Bsa29I ATCGAT 1 cut(s) 489
BsaBI GATNNNNATC 1 cut(s) 468
BsaJI CCNNGG 1 cut(s) 170
Bsc4I CCNNNNNNNGG 1 cut(s) 194
Bse1I ACTGG 2 cut(s) 595, 929
Bse8I GATNNNNATC 1 cut(s) 468
BseCI ATCGAT 1 cut(s) 489
BseDI CCNNGG 1 cut(s) 170
BseGI GGATG 1 cut(s) 469
BseJI GATNNNNATC 1 cut(s) 468
BseLI CCNNNNNNNGG 1 cut(s) 194
BseNI ACTGG 2 cut(s) 595, 929
BseSI GKGCMC 1 cut(s) 1168
Bsh1236I CGCG 1 cut(s) 721
BshVI ATCGAT 1 cut(s) 489
BsiHKAI GWGCWC 1 cut(s) 1168
BslI CCNNNNNNNGG 1 cut(s) 194
BsmAI GTCTC 3 cut(s) 215, 396, 942
BsmBI CGTCTC 1 cut(s) 942
Bsp119I TTCGAA 1 cut(s) 75
Bsp1286I GDGCHC 1 cut(s) 1168
Bsp143I GATC 9 cut(s) 4, 55, 63, 165, 322, 486, 652, 967, 1063
BspACI CCGC 3 cut(s) 561, 613, 719
BspDI ATCGAT 1 cut(s) 489
BspFNI CGCG 1 cut(s) 721
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 4 cut(s) 12, 160, 317, 975
BspT104I TTCGAA 1 cut(s) 75
BsrI ACTGG 2 cut(s) 595, 929
BssECI CCNNGG 1 cut(s) 170
BssMI GATC 9 cut(s) 4, 55, 63, 165, 322, 486, 652, 967, 1063
BssNAI GTATAC 1 cut(s) 646
BssSI CACGAG 2 cut(s) 360, 681
BssT1I CCWWGG 1 cut(s) 170
Bst1107I GTATAC 1 cut(s) 646
Bst2BI CACGAG 2 cut(s) 360, 681
Bst4CI ACNGT 3 cut(s) 287, 634, 982
BstBI TTCGAA 1 cut(s) 75
BstDEI CTNAG 1 cut(s) 845
BstF5I GGATG 1 cut(s) 469
BstFNI CGCG 1 cut(s) 721
BstH2I RGCGCY 1 cut(s) 155
BstHHI GCGC 1 cut(s) 154
BstKTI GATC 9 cut(s) 7, 58, 66, 168, 325, 489, 655, 970, 1066
BstMAI GTCTC 3 cut(s) 215, 396, 942
BstMBI GATC 9 cut(s) 4, 55, 63, 165, 322, 486, 652, 967, 1063
BstMWI GCNNNNNNNGC 1 cut(s) 160
BstSFI CTRYAG 1 cut(s) 439
BstSLI GKGCMC 1 cut(s) 1168
BstUI CGCG 1 cut(s) 721
BstV2I GAAGAC 1 cut(s) 1087
BstX2I RGATCY 5 cut(s) 4, 55, 165, 322, 967
BstYI RGATCY 5 cut(s) 4, 55, 165, 322, 967
BstZ17I GTATAC 1 cut(s) 646
Bsu15I ATCGAT 1 cut(s) 489
BsuI GTATCC 1 cut(s) 557
BsuTUI ATCGAT 1 cut(s) 489
BtgZI GCGATG 1 cut(s) 1025
BtsCI GGATG 1 cut(s) 469
BtsIMutI CAGTG 4 cut(s) 120, 283, 978, 1036
CfoI GCGC 1 cut(s) 154
Cfr13I GGNCC 2 cut(s) 39, 885
ClaI ATCGAT 1 cut(s) 489
Csp6I GTAC 2 cut(s) 664, 983
CviAII CATG 3 cut(s) 67, 484, 1067
CviJI RGCY 8 cut(s) 30, 36, 46, 125, 130, 175, 310, 337
CviKI_1 RGCY 8 cut(s) 30, 36, 46, 125, 130, 175, 310, 337
CviQI GTAC 2 cut(s) 664, 983
DdeI CTNAG 1 cut(s) 845
DpnI GATC 9 cut(s) 6, 57, 65, 167, 324, 488, 654, 969, 1065
DpnII GATC 9 cut(s) 4, 55, 63, 165, 322, 486, 652, 967, 1063
DriI GACNNNNNGTC 1 cut(s) 883
Eam1105I GACNNNNNGTC 1 cut(s) 883
Eco130I CCWWGG 1 cut(s) 170
Eco47I GGWCC 2 cut(s) 39, 885
Eco57I CTGAAG 1 cut(s) 246
EcoRI GAATTC 2 cut(s) 269, 907
EcoT14I CCWWGG 1 cut(s) 170
ErhI CCWWGG 1 cut(s) 170
Esp3I CGTCTC 1 cut(s) 942
FaeI CATG 3 cut(s) 70, 487, 1070
FalI AAGNNNNNCTT 2 cut(s) 650, 682
FatI CATG 3 cut(s) 66, 483, 1066
FbaI TGATCA 1 cut(s) 63
FblI GTMKAC 1 cut(s) 645
FokI GGATG 1 cut(s) 456
FspBI CTAG 3 cut(s) 122, 327, 971
GlaI GCGC 1 cut(s) 153
GsuI CTGGAG 1 cut(s) 612
HaeII RGCGCY 1 cut(s) 155
HhaI GCGC 1 cut(s) 154
Hin1II CATG 3 cut(s) 70, 487, 1070
Hin6I GCGC 1 cut(s) 152
HinP1I GCGC 1 cut(s) 152
HincII GTYRAC 1 cut(s) 637
HindII GTYRAC 1 cut(s) 637
HindIII AAGCTT 1 cut(s) 44
HinfI GANTC 5 cut(s) 250, 389, 771, 1005, 1059
HpaI GTTAAC 1 cut(s) 637
Hpy166II GTNNAC 4 cut(s) 357, 637, 646, 1166
Hpy188I TCNGA 5 cut(s) 18, 25, 226, 776, 1058
Hpy188III TCNNGA 7 cut(s) 61, 141, 188, 362, 620, 683, 1103
Hpy8I GTNNAC 4 cut(s) 357, 637, 646, 1166
HpyAV CCTTC 3 cut(s) 21, 898, 995
HpyCH4III ACNGT 3 cut(s) 287, 634, 982
HpyCH4IV ACGT 3 cut(s) 79, 783, 879
HpyCH4V TGCA 3 cut(s) 585, 894, 1166
HpyF10VI GCNNNNNNNGC 1 cut(s) 160
HpyF3I CTNAG 1 cut(s) 845
HpySE526I ACGT 3 cut(s) 79, 783, 879
Hsp92II CATG 3 cut(s) 70, 487, 1070
HspAI GCGC 1 cut(s) 152
Ksp22I TGATCA 1 cut(s) 63
KspAI GTTAAC 1 cut(s) 637
Kzo9I GATC 9 cut(s) 4, 55, 63, 165, 322, 486, 652, 967, 1063
LpnPI CCDG 5 cut(s) 22, 201, 576, 910, 1146
LweI GCATC 1 cut(s) 903
MaeI CTAG 3 cut(s) 122, 327, 971
MaeII ACGT 3 cut(s) 79, 783, 879
MaeIII GTNAC 6 cut(s) 281, 497, 674, 779, 823, 875
MalI GATC 9 cut(s) 6, 57, 65, 167, 324, 488, 654, 969, 1065
MboI GATC 9 cut(s) 4, 55, 63, 165, 322, 486, 652, 967, 1063
MboII GAAGA 6 cut(s) 239, 250, 368, 722, 903, 1087
MflI RGATCY 5 cut(s) 4, 55, 165, 322, 967
MhlI GDGCHC 1 cut(s) 1168
MlyI GAGTC 1 cut(s) 398
MnlI CCTC 2 cut(s) 946, 1056
MroXI GAANNNNTTC 1 cut(s) 714
MseI TTAA 8 cut(s) 219, 372, 423, 636, 744, 758, 830, 852
MslI CAYNNNNRTG 2 cut(s) 556, 572
MvnI CGCG 1 cut(s) 721
MwoI GCNNNNNNNGC 1 cut(s) 160
NdeII GATC 9 cut(s) 4, 55, 63, 165, 322, 486, 652, 967, 1063
NlaIII CATG 3 cut(s) 70, 487, 1070
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 5 cut(s) 281, 497, 779, 823, 875
NspV TTCGAA 1 cut(s) 75
PdmI GAANNNNTTC 1 cut(s) 714
PfeI GAWTC 4 cut(s) 250, 771, 1005, 1059
PleI GAGTC 1 cut(s) 397
PpsI GAGTC 1 cut(s) 397
PshBI ATTAAT 2 cut(s) 372, 758
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 2 cut(s) 39, 885
PsuI RGATCY 5 cut(s) 4, 55, 165, 322, 967
RsaI GTAC 2 cut(s) 665, 984
RsaNI GTAC 2 cut(s) 664, 983
RseI CAYNNNNRTG 2 cut(s) 556, 572
SaqAI TTAA 8 cut(s) 219, 372, 423, 636, 744, 758, 830, 852
Sau3AI GATC 9 cut(s) 4, 55, 63, 165, 322, 486, 652, 967, 1063
Sau96I GGNCC 2 cut(s) 39, 885
SchI GAGTC 1 cut(s) 398
SduI GDGCHC 1 cut(s) 1168
SetI ASST 9 cut(s) 38, 48, 82, 127, 199, 339, 786, 882, 976
SfaNI GCATC 1 cut(s) 903
SfcI CTRYAG 1 cut(s) 439
SfuI TTCGAA 1 cut(s) 75
SinI GGWCC 2 cut(s) 39, 885
SmiMI CAYNNNNRTG 2 cut(s) 556, 572
SmlI CTYRAG 1 cut(s) 31
SmoI CTYRAG 1 cut(s) 31
SsiI CCGC 3 cut(s) 561, 613, 719
SspI AATATT 1 cut(s) 601
SspMI CTAG 3 cut(s) 122, 327, 971
StyI CCWWGG 1 cut(s) 170
TaaI ACNGT 3 cut(s) 287, 634, 982
TaiI ACGT 3 cut(s) 82, 786, 882
TaqI TCGA 6 cut(s) 75, 489, 555, 629, 999, 1062
TatI WGTACW 2 cut(s) 663, 982
TfiI GAWTC 4 cut(s) 250, 771, 1005, 1059
Tru1I TTAA 8 cut(s) 219, 372, 423, 636, 744, 758, 830, 852
Tru9I TTAA 8 cut(s) 219, 372, 423, 636, 744, 758, 830, 852
TscAI CASTG 4 cut(s) 120, 290, 985, 1036
TseFI GTSAC 5 cut(s) 281, 497, 779, 823, 875
Tsp45I GTSAC 5 cut(s) 281, 497, 779, 823, 875
TspDTI ATGAA 2 cut(s) 362, 471
TspGWI ACGGA 1 cut(s) 369
TspRI CASTG 4 cut(s) 120, 290, 985, 1036
VneI GTGCAC 1 cut(s) 1164
VpaK11BI GGWCC 2 cut(s) 39, 885
VspI ATTAAT 2 cut(s) 372, 758
XapI RAATTY 5 cut(s) 269, 349, 907, 1015, 1183
XcmI CCANNNNNNNNNTGG 1 cut(s) 535
XmiI GTMKAC 1 cut(s) 645
XmnI GAANNNNTTC 1 cut(s) 714
XspI CTAG 3 cut(s) 122, 327, 971
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.