AT2G04840

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0000658
Physical Location & Seq
Forward (+)
1697009 .. 1698240
1232 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G04840.1

Sequence Viewer

Length: 1170 bp
ATGGGGAACGGCTTGATGAGCAAGAAGAAGAAGAAGAAGAACAACAAGTACAAGAAGAAGAGACCAAACGTGTCTAGAAATTGCGATTGGTCCAAGTTTTGTTCCGATGTTTTACGCAAGATTTTCGAAACCTTAAAGAGCCCTATTGATTCTCACCGCGCGTTTGCTCGAATTAGTATTCAGTTTGGAAAACATGCGCGAACAAGCCTCCATGTCCATGGCGATTCATACACCAAGGTGATTCTCCCACCCTCCGGAAACTGTCTCCTGATGGTCGATCGTCGTCTCAAATTCTATATTTTGAACCTTTTGACGCGGACAACGATCAATCTTCCATCAATGTCACTAATACGTGGAGGACAAGTATTCTTTAAACCTCAAAATGAATGGTCCAATGATTCTGGATGCTTCTTCGGGCCTTCTCGTAATGGTAACGTGTCATATGACTTTGACTCTGTTGAATGGAAAAACTCGGTAGCTGTTCTATGGATAAACGAGACAACAGGAGATTATGTCGTTGCTTGGACATTCATACAGCATTACTTGTTCTTCTACAAGTCAGGATATTGGTCTTGGTGTAATCTGAATCATAATGGGAAAAGTTTGGTTGTGTTTGATATGGCATGTGAGAACAATAAGCTTTATCTGTACACAGCTAATCATCATATCAGGATTTTTGATTTCTCTGGAGCTTATCCTATGGAAAAGAAAACCGAAAACCCGTATTGGAAATATCTGTTTAACGCGGTTGAGGAACCGTGGGAATACGTTTGGAAGAGGAAAATAGCGATTCAGAAATCATCAGGTGAGGTTTTGATCATCTTGAGCCTTAAACAAGTGTTTTACGAAGCAGAGAGACTTTTGTTTTACATCTTTAAGATGAATCTTGAGAGTTTCAAGTGGGAAAGAGTTTATTCTCTTGGAGATGAGATGTTGGTTTTTGGTCAAGGTGTTACAATACCTACTGCTCTTAAAGATCTTGGTGATGGAATCAAGAGTGATTCAATCTATTTCGTCGATAAAGACGTCTGGCCAGATCATCAAGATCATGATCGTCGTGTTTCGAATTGCGGTGTCTTCAATATTGCCACAAGTAAGATCAAATGGCCTACGAAAAAATATCGTTCCGTAAATGAAACTCAGTGGTTTGTGCTCGGAGTTGCTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

389

Amino Acids

45.57

Weight (kDa)

9.42

Isoelectric Point (pI)

33.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 72 - 361 2.2e-39 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1029
AccII CGCG 5 cut(s) 159, 161, 199, 316, 746
AccIII TCCGGA 1 cut(s) 254
AciI CCGC 4 cut(s) 157, 316, 746, 1071
AcoI YGGCCR 1 cut(s) 1031
AcsI RAATTY 1 cut(s) 290
AcyI GRCGYC 1 cut(s) 1026
AfaI GTAC 2 cut(s) 50, 650
AfiI CCNNNNNNNGG 1 cut(s) 254
AflIII ACRYGT 2 cut(s) 69, 435
AgsI TTSAA 5 cut(s) 304, 461, 898, 1005, 1081
AjuI GAANNNNNNNTTGG 2 cut(s) 70, 102
AleI CACNNNNGTG 1 cut(s) 236
AluBI AGCT 4 cut(s) 479, 640, 656, 692
AluI AGCT 4 cut(s) 479, 640, 656, 692
Alw21I GWGCWC 1 cut(s) 1155
Alw26I GTCTC 5 cut(s) 55, 269, 290, 491, 850
Aor13HI TCCGGA 1 cut(s) 254
AoxI GGCC 3 cut(s) 416, 1031, 1106
ApoI RAATTY 1 cut(s) 290
AspLEI GCGC 2 cut(s) 161, 199
AspS9I GGNCC 3 cut(s) 90, 390, 416
AsuHPI GGTGA 4 cut(s) 146, 250, 818, 995
AsuII TTCGAA 2 cut(s) 126, 1064
AvaII GGWCC 2 cut(s) 90, 390
BalI TGGCCA 1 cut(s) 1033
BanII GRGCYC 1 cut(s) 143
BbsI GAAGAC 1 cut(s) 1069
Bbv12I GWGCWC 1 cut(s) 1155
BccI CCATC 3 cut(s) 265, 343, 980
BceAI ACGGC 1 cut(s) 25
BcgI CGANNNNNNTGC 2 cut(s) 106, 140
BclI TGATCA 1 cut(s) 816
BcoDI GTCTC 5 cut(s) 55, 269, 290, 491, 850
BfaI CTAG 1 cut(s) 75
BglII AGATCT 1 cut(s) 976
Bme18I GGWCC 2 cut(s) 90, 390
BmgT120I GGNCC 3 cut(s) 90, 390, 416
BmiI GGNNCC 1 cut(s) 756
BmsI GCATC 1 cut(s) 395
BpiI GAAGAC 1 cut(s) 1069
BpmI CTGGAG 1 cut(s) 708
Bpu14I TTCGAA 2 cut(s) 126, 1064
BpuEI CTTGAG 2 cut(s) 844, 908
BsaAI YACGTR 1 cut(s) 353
BsaBI GATNNNNATC 1 cut(s) 1050
BsaHI GRCGYC 1 cut(s) 1026
BsaI GGTCTC 1 cut(s) 55
BsaJI CCNNGG 3 cut(s) 217, 234, 758
BsaWI WCCGGW 1 cut(s) 254
BsaXI ACNNNNNCTCC 4 cut(s) 348, 378, 498, 528
Bsc4I CCNNNNNNNGG 1 cut(s) 254
Bse8I GATNNNNATC 1 cut(s) 1050
BseAI TCCGGA 1 cut(s) 254
BseDI CCNNGG 3 cut(s) 217, 234, 758
BseGI GGATG 1 cut(s) 410
BseJI GATNNNNATC 1 cut(s) 1050
BseLI CCNNNNNNNGG 1 cut(s) 254
BseMII CTCAG 1 cut(s) 1154
Bsh1236I CGCG 5 cut(s) 159, 161, 199, 316, 746
Bsh1285I CGRYCG 1 cut(s) 280
BshFI GGCC 3 cut(s) 418, 1033, 1108
BsiEI CGRYCG 1 cut(s) 280
BsiHKAI GWGCWC 1 cut(s) 1155
BsiSI CCGG 1 cut(s) 255
BslI CCNNNNNNNGG 1 cut(s) 254
BsmAI GTCTC 5 cut(s) 55, 269, 290, 491, 850
BsmBI CGTCTC 1 cut(s) 290
BsnI GGCC 3 cut(s) 418, 1033, 1108
Bso31I GGTCTC 1 cut(s) 55
Bsp119I TTCGAA 2 cut(s) 126, 1064
Bsp1286I GDGCHC 2 cut(s) 143, 1155
Bsp13I TCCGGA 1 cut(s) 254
Bsp1407I TGTACA 1 cut(s) 648
Bsp143I GATC 8 cut(s) 277, 324, 816, 976, 1036, 1045, 1051, 1098
Bsp19I CCATGG 1 cut(s) 217
BspACI CCGC 4 cut(s) 157, 316, 746, 1071
BspANI GGCC 3 cut(s) 418, 1033, 1108
BspCNI CTCAG 1 cut(s) 1153
BspEI TCCGGA 1 cut(s) 254
BspFNI CGCG 5 cut(s) 159, 161, 199, 316, 746
BspHI TCATGA 1 cut(s) 1048
BspLI GGNNCC 1 cut(s) 756
BspT104I TTCGAA 2 cut(s) 126, 1064
BspTNI GGTCTC 1 cut(s) 55
BsrGI TGTACA 1 cut(s) 648
BssECI CCNNGG 3 cut(s) 217, 234, 758
BssMI GATC 8 cut(s) 277, 324, 816, 976, 1036, 1045, 1051, 1098
BssNI GRCGYC 1 cut(s) 1026
BssT1I CCWWGG 2 cut(s) 217, 234
Bst4CI ACNGT 2 cut(s) 263, 759
Bst6I CTCTTC 2 cut(s) 53, 770
BstACI GRCGYC 1 cut(s) 1026
BstAUI TGTACA 1 cut(s) 648
BstBAI YACGTR 1 cut(s) 353
BstBI TTCGAA 2 cut(s) 126, 1064
BstDEI CTNAG 1 cut(s) 1140
BstDSI CCRYGG 2 cut(s) 217, 758
BstF5I GGATG 1 cut(s) 410
BstFNI CGCG 5 cut(s) 159, 161, 199, 316, 746
BstHHI GCGC 2 cut(s) 161, 199
BstKTI GATC 8 cut(s) 280, 327, 819, 979, 1039, 1048, 1054, 1101
BstMAI GTCTC 5 cut(s) 55, 269, 290, 491, 850
BstMBI GATC 8 cut(s) 277, 324, 816, 976, 1036, 1045, 1051, 1098
BstMCI CGRYCG 1 cut(s) 280
BstMWI GCNNNNNNNGC 1 cut(s) 18
BstNSI RCATGY 2 cut(s) 197, 627
BstUI CGCG 5 cut(s) 159, 161, 199, 316, 746
BstV2I GAAGAC 1 cut(s) 1069
BstX2I RGATCY 1 cut(s) 976
BstXI CCANNNNNNTGG 1 cut(s) 218
BstYI RGATCY 1 cut(s) 976
BsuRI GGCC 3 cut(s) 418, 1033, 1108
BtgI CCRYGG 2 cut(s) 217, 758
BtsCI GGATG 1 cut(s) 410
BtsIMutI CAGTG 1 cut(s) 1148
CciI TCATGA 1 cut(s) 1048
CfoI GCGC 2 cut(s) 161, 199
Cfr13I GGNCC 3 cut(s) 90, 390, 416
CseI GACGC 1 cut(s) 322
Csp6I GTAC 2 cut(s) 49, 649
CviAII CATG 5 cut(s) 194, 212, 218, 624, 1049
CviQI GTAC 2 cut(s) 49, 649
DdeI CTNAG 1 cut(s) 1140
DpnI GATC 8 cut(s) 279, 326, 818, 978, 1038, 1047, 1053, 1100
DpnII GATC 8 cut(s) 277, 324, 816, 976, 1036, 1045, 1051, 1098
DraI TTTAAA 1 cut(s) 373
EaeI YGGCCR 1 cut(s) 1031
Eam1104I CTCTTC 2 cut(s) 53, 770
EarI CTCTTC 2 cut(s) 53, 770
Eco130I CCWWGG 2 cut(s) 217, 234
Eco24I GRGCYC 1 cut(s) 143
Eco31I GGTCTC 1 cut(s) 55
Eco47I GGWCC 2 cut(s) 90, 390
EcoT14I CCWWGG 2 cut(s) 217, 234
EcoT38I GRGCYC 1 cut(s) 143
ErhI CCWWGG 2 cut(s) 217, 234
Esp3I CGTCTC 1 cut(s) 290
FaeI CATG 5 cut(s) 197, 215, 221, 627, 1052
FatI CATG 5 cut(s) 193, 211, 217, 623, 1048
FauNDI CATATG 1 cut(s) 442
FbaI TGATCA 1 cut(s) 816
FokI GGATG 1 cut(s) 417
FriOI GRGCYC 1 cut(s) 143
FspBI CTAG 1 cut(s) 75
GlaI GCGC 2 cut(s) 160, 198
GsuI CTGGAG 1 cut(s) 708
HaeIII GGCC 3 cut(s) 418, 1033, 1108
HapII CCGG 1 cut(s) 255
HgaI GACGC 1 cut(s) 322
HhaI GCGC 2 cut(s) 161, 199
Hin1I GRCGYC 1 cut(s) 1026
Hin1II CATG 5 cut(s) 197, 215, 221, 627, 1052
Hin6I GCGC 2 cut(s) 159, 197
HinP1I GCGC 2 cut(s) 159, 197
HindIII AAGCTT 1 cut(s) 638
HpaII CCGG 1 cut(s) 255
HphI GGTGA 4 cut(s) 146, 250, 818, 995
Hpy166II GTNNAC 1 cut(s) 651
Hpy188I TCNGA 4 cut(s) 106, 585, 795, 1157
Hpy8I GTNNAC 1 cut(s) 651
Hpy99I CGWCG 3 cut(s) 285, 1019, 1059
HpyAV CCTTC 1 cut(s) 429
HpyCH4III ACNGT 2 cut(s) 263, 759
HpyCH4IV ACGT 5 cut(s) 69, 352, 435, 768, 1026
HpyF10VI GCNNNNNNNGC 1 cut(s) 18
HpyF3I CTNAG 1 cut(s) 1140
HpySE526I ACGT 5 cut(s) 69, 352, 435, 768, 1026
Hsp92I GRCGYC 1 cut(s) 1026
Hsp92II CATG 5 cut(s) 197, 215, 221, 627, 1052
HspAI GCGC 2 cut(s) 159, 197
Kpn2I TCCGGA 1 cut(s) 254
Ksp22I TGATCA 1 cut(s) 816
Kzo9I GATC 8 cut(s) 277, 324, 816, 976, 1036, 1045, 1051, 1098
LmnI GCTCC 1 cut(s) 689
LweI GCATC 1 cut(s) 395
MaeI CTAG 1 cut(s) 75
MaeII ACGT 5 cut(s) 69, 352, 435, 768, 1026
MaeIII GTNAC 3 cut(s) 342, 431, 952
MalI GATC 8 cut(s) 279, 326, 818, 978, 1038, 1047, 1053, 1100
MboI GATC 8 cut(s) 277, 324, 816, 976, 1036, 1045, 1051, 1098
MflI RGATCY 1 cut(s) 976
MhlI GDGCHC 2 cut(s) 143, 1155
MlsI TGGCCA 1 cut(s) 1033
MluCI AATT 4 cut(s) 79, 171, 290, 1066
MluNI TGGCCA 1 cut(s) 1033
MlyI GAGTC 1 cut(s) 446
MnlI CCTC 7 cut(s) 218, 262, 350, 387, 745, 771, 802
Mox20I TGGCCA 1 cut(s) 1033
MroI TCCGGA 1 cut(s) 254
MscI TGGCCA 1 cut(s) 1033
MseI TTAA 6 cut(s) 134, 372, 741, 831, 876, 972
MslI CAYNNNNRTG 2 cut(s) 216, 236
Msp20I TGGCCA 1 cut(s) 1033
MspI CCGG 1 cut(s) 255
MvnI CGCG 5 cut(s) 159, 161, 199, 316, 746
MwoI GCNNNNNNNGC 1 cut(s) 18
NcoI CCATGG 1 cut(s) 217
NdeI CATATG 1 cut(s) 442
NdeII GATC 8 cut(s) 277, 324, 816, 976, 1036, 1045, 1051, 1098
NlaIII CATG 5 cut(s) 197, 215, 221, 627, 1052
NlaIV GGNNCC 1 cut(s) 756
NmuCI GTSAC 1 cut(s) 342
NspI RCATGY 2 cut(s) 197, 627
NspV TTCGAA 2 cut(s) 126, 1064
OliI CACNNNNGTG 1 cut(s) 236
PagI TCATGA 1 cut(s) 1048
PcsI WCGNNNNNNNCGW 2 cut(s) 320, 1023
PfeI GAWTC 9 cut(s) 149, 224, 241, 398, 586, 790, 883, 990, 1001
Ple19I CGATCG 1 cut(s) 280
PleI GAGTC 1 cut(s) 446
PpsI GAGTC 1 cut(s) 446
Ppu21I YACGTR 1 cut(s) 353
PspN4I GGNNCC 1 cut(s) 756
PspPI GGNCC 3 cut(s) 90, 390, 416
PsrI GAACNNNNNNTAC 2 cut(s) 32, 64
PsuI RGATCY 1 cut(s) 976
PvuI CGATCG 1 cut(s) 280
RsaI GTAC 2 cut(s) 50, 650
RsaNI GTAC 2 cut(s) 49, 649
RseI CAYNNNNRTG 2 cut(s) 216, 236
SaqAI TTAA 6 cut(s) 134, 372, 741, 831, 876, 972
Sau3AI GATC 8 cut(s) 277, 324, 816, 976, 1036, 1045, 1051, 1098
Sau96I GGNCC 3 cut(s) 90, 390, 416
SchI GAGTC 1 cut(s) 446
SduI GDGCHC 2 cut(s) 143, 1155
SfaNI GCATC 1 cut(s) 395
SfuI TTCGAA 2 cut(s) 126, 1064
SinI GGWCC 2 cut(s) 90, 390
SmiMI CAYNNNNRTG 2 cut(s) 216, 236
SmlI CTYRAG 2 cut(s) 823, 887
SmoI CTYRAG 2 cut(s) 823, 887
Sse9I AATT 4 cut(s) 79, 171, 290, 1066
SsiI CCGC 4 cut(s) 157, 316, 746, 1071
SspI AATATT 1 cut(s) 1084
SspMI CTAG 1 cut(s) 75
StyI CCWWGG 2 cut(s) 217, 234
TaaI ACNGT 2 cut(s) 263, 759
TaiI ACGT 5 cut(s) 72, 355, 438, 771, 1029
TaqI TCGA 5 cut(s) 126, 169, 276, 1017, 1064
TasI AATT 4 cut(s) 79, 171, 290, 1066
TatI WGTACW 2 cut(s) 48, 648
TfiI GAWTC 9 cut(s) 149, 224, 241, 398, 586, 790, 883, 990, 1001
Tru1I TTAA 6 cut(s) 134, 372, 741, 831, 876, 972
Tru9I TTAA 6 cut(s) 134, 372, 741, 831, 876, 972
TscAI CASTG 1 cut(s) 1148
TseFI GTSAC 1 cut(s) 342
Tsp45I GTSAC 1 cut(s) 342
TspDTI ATGAA 5 cut(s) 216, 399, 520, 896, 1149
TspGWI ACGGA 1 cut(s) 1117
TspRI CASTG 1 cut(s) 1148
VpaK11BI GGWCC 2 cut(s) 90, 390
XapI RAATTY 1 cut(s) 290
XbaI TCTAGA 1 cut(s) 74
XceI RCATGY 2 cut(s) 197, 627
XspI CTAG 1 cut(s) 75
ZraI GACGTC 1 cut(s) 1027
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.