AT5G25300

F-box domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000658
Physical Location & Seq
Forward (+)
8780258 .. 8783093
2836 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G25300.1

Sequence Viewer

Length: 1434 bp
ATGGATACTACTAATTCTAGCCGCTGGTCAGAGCTTCCTATAGACATCTTGAGATCTCTCCTCGAACAAAAAGGTGGTTGTGCTATATACAACCCGGACGAAGCTAAAGATTACAAAACAAAAAGAGACTCCTCAGGGATTCGATTCTTGGCAAACTCAGTTAACTGGTTCTTGGTGCTAGATTCTCGTTCCAATCTCTATATCATAGATGTGTTTAGCGAGAAAAAGATCGATCTTCCACCCCTAGAATCAATCAAGAATGGCCTTTATAGTCTTGAGCAAGTAGGAGACGAGAAGAAAAAAGAATACATTGTAGTGTGGTTTTTCGACAAAGGTACCGAATATATAGCCTTTTGCAAGAACGGGGAAGATCATTACCGCGACATTCCGATACGAAAAAATGTTTGTAAAGAGTTACAAGACTTATATGATATGTTACTCCATGGTGGTAACAATCTTTATATCTCAAAGACTCGTCAATCTATTCGGAAGCTTAGATTTTTTGAAGAAGAAGGTTTCATTGATGTCGACAATAGTGAGATATTACCATTTCGTAAAATCTCCTTTTACATTGATGGAGCAAGATTTAGTAATAACATTGCCGTTACAACATCAAGAGAGGTTTTGTTGGTTCAGAACTTCTTTTACGAGACAACAAGATATAGGAGCTTCCGTTTGTACAAGAAAGATCCTAATCCGGACTTGAATGCTACTATCAACAACCCCTACCCGATGGTTGAGTTATGTTCTCTAGGTGATGTCTCTGCTTCTGGATTTGGTGACCGGAAACGTTCTTGTCTGGACATGTGTATGTTCAATCTTGTGACAAAGAAACTTAAACGATTCCCGGATCTTTCCAACTTAAAGCTCACGGATGCTCGATGTACTACGACAAGCAGTGGCAACAATTGGAAATTTTCGATTATTGTTTCCGAGGTCATTGATTCTAAATTTTTGGAACTTCGATTTGCAGATCTTGATTCTGAATCTCAACAACAAACAAAACTGAAGTTCTCAATGCTCAAGAGTGAATTTGTAGATATGAATAAGGAAGATTCTCAAATTGAGATCAATGAGAAAGAGACAAAAATCAACCAAGAACATGATCAAAGTGATGAAACTCAAGCTAAACGTAGGAGAAGTCTAACTTCTTCTGTTTGGCAAGATCTTGTATCAGTAGGGGGAGAAGCAAATGTTAAAGAAATAGATAGAAGCATGGAGACTCCTAATTCTAGTCCCCCCTGGTCGGAGCTCCCTGGTGACATATTAAGATCTGTGTTCGAACGTTTGAGTTTTGTTGATTTCCAGCGCGCTAAGCAAACATGTCCGATTAAAAGATCTAAGTCTAACTGTCTAAGGCTTTGGCTCATAACATTTGGTGGTCTTGAGAGACTTCCACACTTATCTTCTGTTTGTTTCATATCATGGACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box

Protein Analysis

477

Amino Acids

55.26

Weight (kDa)

6.52

Isoelectric Point (pI)

51.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 30 - 253 4.5e-26 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 335
AccB1I GGYRCC 1 cut(s) 335
AccI GTMKAC 1 cut(s) 528
AccII CGCG 2 cut(s) 381, 1311
AccIII TCCGGA 1 cut(s) 697
AciI CCGC 2 cut(s) 22, 379
AclI AACGTT 2 cut(s) 790, 1285
AclWI GGATC 2 cut(s) 683, 858
AcsI RAATTY 3 cut(s) 914, 950, 1031
AcuI CTGAAG 1 cut(s) 1028
AfaI GTAC 3 cut(s) 337, 680, 886
AfiI CCNNNNNNNGG 1 cut(s) 1246
AflIII ACRYGT 2 cut(s) 804, 1322
AgsI TTSAA 3 cut(s) 506, 706, 817
AjnI CCWGG 2 cut(s) 1241, 1255
AluBI AGCT 7 cut(s) 34, 104, 493, 669, 868, 1127, 1252
AluI AGCT 7 cut(s) 34, 104, 493, 669, 868, 1127, 1252
Alw21I GWGCWC 1 cut(s) 1254
Alw26I GTCTC 7 cut(s) 120, 282, 644, 766, 1076, 1214, 1384
AlwI GGATC 2 cut(s) 683, 858
Aor13HI TCCGGA 1 cut(s) 697
AoxI GGCC 1 cut(s) 262
ApoI RAATTY 3 cut(s) 914, 950, 1031
Asp718I GGTACC 1 cut(s) 335
AspLEI GCGC 2 cut(s) 1311, 1313
AspS9I GGNCC 1 cut(s) 1428
AsuC2I CCSGG 2 cut(s) 95, 848
AsuHPI GGTGA 3 cut(s) 767, 791, 1271
AsuII TTCGAA 1 cut(s) 1281
AvaII GGWCC 1 cut(s) 1428
AxyI CCTNAGG 1 cut(s) 133
BaeI ACNNNNGTAYC 2 cut(s) 319, 352
BanI GGYRCC 1 cut(s) 335
BanII GRGCYC 1 cut(s) 1254
Bbv12I GWGCWC 1 cut(s) 1254
BccI CCATC 2 cut(s) 569, 727
BceAI ACGGC 1 cut(s) 587
BciT130I CCWGG 2 cut(s) 1243, 1257
BclI TGATCA 1 cut(s) 1105
BcnI CCSGG 2 cut(s) 95, 848
BcoDI GTCTC 7 cut(s) 120, 282, 644, 766, 1076, 1214, 1384
BfaI CTAG 5 cut(s) 18, 179, 245, 752, 1233
BfmI CTRYAG 1 cut(s) 39
BglII AGATCT 5 cut(s) 53, 973, 1165, 1271, 1337
BisI GCNGC 1 cut(s) 22
BlpI GCTNAGC 1 cut(s) 1314
BlsI GCNGC 1 cut(s) 23
Bme1390I CCNGG 4 cut(s) 95, 848, 1243, 1257
Bme18I GGWCC 1 cut(s) 1428
BmgT120I GGNCC 1 cut(s) 1428
BmiI GGNNCC 1 cut(s) 337
BmrFI CCNGG 4 cut(s) 95, 848, 1243, 1257
BmsI GCATC 1 cut(s) 865
Bpu1102I GCTNAGC 1 cut(s) 1314
Bpu14I TTCGAA 1 cut(s) 1281
BpuEI CTTGAG 5 cut(s) 70, 296, 1007, 1107, 1406
BpuMI CCSGG 2 cut(s) 95, 848
Bsa29I ATCGAT 1 cut(s) 231
BsaBI GATNNNNATC 1 cut(s) 693
BsaJI CCNNGG 4 cut(s) 442, 933, 1241, 1255
BsaWI WCCGGW 2 cut(s) 697, 783
BsaXI ACNNNNNCTCC 2 cut(s) 658, 688
Bsc4I CCNNNNNNNGG 1 cut(s) 1246
Bse1I ACTGG 1 cut(s) 170
Bse21I CCTNAGG 1 cut(s) 133
Bse3DI GCAATG 1 cut(s) 597
Bse8I GATNNNNATC 1 cut(s) 693
BseAI TCCGGA 1 cut(s) 697
BseBI CCWGG 2 cut(s) 1243, 1257
BseCI ATCGAT 1 cut(s) 231
BseDI CCNNGG 4 cut(s) 442, 933, 1241, 1255
BseGI GGATG 1 cut(s) 880
BseJI GATNNNNATC 1 cut(s) 693
BseLI CCNNNNNNNGG 1 cut(s) 1246
BseMI GCAATG 1 cut(s) 597
BseMII CTCAG 2 cut(s) 147, 171
BseNI ACTGG 1 cut(s) 170
BsePI GCGCGC 1 cut(s) 1309
BseRI GAGGAG 2 cut(s) 50, 121
Bsh1236I CGCG 2 cut(s) 381, 1311
BshFI GGCC 1 cut(s) 264
BshNI GGYRCC 1 cut(s) 335
BshVI ATCGAT 1 cut(s) 231
BsiHKAI GWGCWC 1 cut(s) 1254
BsiSI CCGG 4 cut(s) 95, 698, 784, 848
BslFI GGGAC 1 cut(s) 1221
BslI CCNNNNNNNGG 1 cut(s) 1246
BsmAI GTCTC 7 cut(s) 120, 282, 644, 766, 1076, 1214, 1384
BsmBI CGTCTC 1 cut(s) 282
BsmFI GGGAC 1 cut(s) 1221
BsmI GAATGC 1 cut(s) 712
BsnI GGCC 1 cut(s) 264
Bsp119I TTCGAA 1 cut(s) 1281
Bsp1286I GDGCHC 1 cut(s) 1254
Bsp13I TCCGGA 1 cut(s) 697
Bsp1407I TGTACA 1 cut(s) 678
Bsp1720I GCTNAGC 1 cut(s) 1314
Bsp19I CCATGG 1 cut(s) 442
BspACI CCGC 2 cut(s) 22, 379
BspANI GGCC 1 cut(s) 264
BspCNI CTCAG 2 cut(s) 146, 170
BspDI ATCGAT 1 cut(s) 231
BspEI TCCGGA 1 cut(s) 697
BspFNI CGCG 2 cut(s) 381, 1311
BspLI GGNNCC 1 cut(s) 337
BspPI GGATC 2 cut(s) 683, 858
BspT104I TTCGAA 1 cut(s) 1281
BspT107I GGYRCC 1 cut(s) 335
BsrDI GCAATG 1 cut(s) 597
BsrGI TGTACA 1 cut(s) 678
BsrI ACTGG 1 cut(s) 170
BssECI CCNNGG 4 cut(s) 442, 933, 1241, 1255
BssHII GCGCGC 1 cut(s) 1309
BssT1I CCWWGG 1 cut(s) 442
Bst2UI CCWGG 2 cut(s) 1243, 1257
Bst4CI ACNGT 1 cut(s) 1352
BstAUI TGTACA 1 cut(s) 678
BstBI TTCGAA 1 cut(s) 1281
BstC8I GCNNGC 1 cut(s) 1311
BstDEI CTNAG 6 cut(s) 133, 157, 494, 1314, 1341, 1355
BstDSI CCRYGG 1 cut(s) 442
BstEII GGTNACC 1 cut(s) 779
BstF5I GGATG 1 cut(s) 880
BstFNI CGCG 2 cut(s) 381, 1311
BstHHI GCGC 2 cut(s) 1311, 1313
BstMAI GTCTC 7 cut(s) 120, 282, 644, 766, 1076, 1214, 1384
BstMWI GCNNNNNNNGC 1 cut(s) 1315
BstNI CCWGG 2 cut(s) 1243, 1257
BstNSI RCATGY 2 cut(s) 808, 1326
BstPI GGTNACC 1 cut(s) 779
BstSCI CCNGG 4 cut(s) 93, 846, 1241, 1255
BstSFI CTRYAG 1 cut(s) 39
BstUI CGCG 2 cut(s) 381, 1311
BstX2I RGATCY 7 cut(s) 53, 688, 850, 973, 1165, 1271, 1337
BstYI RGATCY 7 cut(s) 53, 688, 850, 973, 1165, 1271, 1337
Bsu15I ATCGAT 1 cut(s) 231
Bsu36I CCTNAGG 1 cut(s) 133
BsuRI GGCC 1 cut(s) 264
BsuTUI ATCGAT 1 cut(s) 231
BtgI CCRYGG 1 cut(s) 442
BtsCI GGATG 1 cut(s) 880
BtsI GCAGTG 1 cut(s) 904
BtsIMutI CAGTG 1 cut(s) 904
Cac8I GCNNGC 1 cut(s) 1311
CfoI GCGC 2 cut(s) 1311, 1313
Cfr13I GGNCC 1 cut(s) 1428
ClaI ATCGAT 1 cut(s) 231
Csp6I GTAC 3 cut(s) 336, 679, 885
CviAII CATG 6 cut(s) 443, 805, 1103, 1216, 1323, 1425
CviQI GTAC 3 cut(s) 336, 679, 885
DdeI CTNAG 6 cut(s) 133, 157, 494, 1314, 1341, 1355
Ecl136II GAGCTC 1 cut(s) 1252
Eco130I CCWWGG 1 cut(s) 442
Eco24I GRGCYC 1 cut(s) 1254
Eco47I GGWCC 1 cut(s) 1428
Eco53kI GAGCTC 1 cut(s) 1252
Eco57I CTGAAG 1 cut(s) 1028
Eco81I CCTNAGG 1 cut(s) 133
Eco91I GGTNACC 1 cut(s) 779
EcoICRI GAGCTC 1 cut(s) 1252
EcoO65I GGTNACC 1 cut(s) 779
EcoRII CCWGG 2 cut(s) 1241, 1255
EcoT14I CCWWGG 1 cut(s) 442
EcoT38I GRGCYC 1 cut(s) 1254
ErhI CCWWGG 1 cut(s) 442
Esp3I CGTCTC 1 cut(s) 282
FaeI CATG 6 cut(s) 446, 808, 1106, 1219, 1326, 1428
FalI AAGNNNNNCTT 2 cut(s) 1132, 1164
FaqI GGGAC 1 cut(s) 1221
FatI CATG 6 cut(s) 442, 804, 1102, 1215, 1322, 1424
FbaI TGATCA 1 cut(s) 1105
FblI GTMKAC 1 cut(s) 528
Fnu4HI GCNGC 1 cut(s) 22
FokI GGATG 1 cut(s) 887
FriOI GRGCYC 1 cut(s) 1254
Fsp4HI GCNGC 1 cut(s) 22
FspBI CTAG 5 cut(s) 18, 179, 245, 752, 1233
GlaI GCGC 2 cut(s) 1310, 1312
GluI GCNGC 1 cut(s) 22
HaeIII GGCC 1 cut(s) 264
HapII CCGG 4 cut(s) 95, 698, 784, 848
HhaI GCGC 2 cut(s) 1311, 1313
Hin1II CATG 6 cut(s) 446, 808, 1106, 1219, 1326, 1428
Hin6I GCGC 2 cut(s) 1309, 1311
HinP1I GCGC 2 cut(s) 1309, 1311
HincII GTYRAC 2 cut(s) 163, 529
HindII GTYRAC 2 cut(s) 163, 529
HindIII AAGCTT 1 cut(s) 491
HpaI GTTAAC 1 cut(s) 163
HpaII CCGG 4 cut(s) 95, 698, 784, 848
HphI GGTGA 3 cut(s) 767, 791, 1271
Hpy166II GTNNAC 2 cut(s) 163, 529
Hpy188I TCNGA 8 cut(s) 31, 390, 489, 636, 934, 985, 1249, 1329
Hpy8I GTNNAC 2 cut(s) 163, 529
HpyAV CCTTC 1 cut(s) 506
HpyCH4III ACNGT 1 cut(s) 1352
HpyCH4IV ACGT 3 cut(s) 790, 1132, 1285
HpyCH4V TGCA 2 cut(s) 357, 971
HpyF10VI GCNNNNNNNGC 1 cut(s) 1315
HpyF3I CTNAG 6 cut(s) 133, 157, 494, 1314, 1341, 1355
HpySE526I ACGT 3 cut(s) 790, 1132, 1285
Hsp92II CATG 6 cut(s) 446, 808, 1106, 1219, 1326, 1428
HspAI GCGC 2 cut(s) 1309, 1311
Kpn2I TCCGGA 1 cut(s) 697
KpnI GGTACC 1 cut(s) 339
Ksp22I TGATCA 1 cut(s) 1105
KspAI GTTAAC 1 cut(s) 163
LmnI GCTCC 4 cut(s) 578, 666, 1249, 1257
LweI GCATC 1 cut(s) 865
MaeI CTAG 5 cut(s) 18, 179, 245, 752, 1233
MaeII ACGT 3 cut(s) 790, 1132, 1285
MaeIII GTNAC 7 cut(s) 414, 435, 449, 604, 779, 823, 1259
MboII GAAGA 8 cut(s) 227, 307, 380, 518, 521, 1064, 1143, 1398
MfeI CAATTG 1 cut(s) 907
MflI RGATCY 7 cut(s) 53, 688, 850, 973, 1165, 1271, 1337
MhlI GDGCHC 1 cut(s) 1254
MluCI AATT 7 cut(s) 13, 907, 914, 950, 1031, 1062, 1228
MlyI GAGTC 3 cut(s) 122, 466, 1216
MmeI TCCRAC 2 cut(s) 882, 1227
MnlI CCTC 4 cut(s) 71, 142, 613, 928
MroI TCCGGA 1 cut(s) 697
MseI TTAA 6 cut(s) 162, 837, 863, 1197, 1268, 1332
MslI CAYNNNNRTG 3 cut(s) 209, 314, 809
MspA1I CMGCKG 1 cut(s) 24
MspI CCGG 4 cut(s) 95, 698, 784, 848
MspR9I CCNGG 4 cut(s) 95, 848, 1243, 1257
MunI CAATTG 1 cut(s) 907
Mva1269I GAATGC 1 cut(s) 712
MvaI CCWGG 2 cut(s) 1243, 1257
MvnI CGCG 2 cut(s) 381, 1311
MwoI GCNNNNNNNGC 1 cut(s) 1315
NciI CCSGG 2 cut(s) 95, 848
NcoI CCATGG 1 cut(s) 442
NlaIII CATG 6 cut(s) 446, 808, 1106, 1219, 1326, 1428
NlaIV GGNNCC 1 cut(s) 337
NmuCI GTSAC 3 cut(s) 779, 823, 1259
NspI RCATGY 2 cut(s) 808, 1326
NspV TTCGAA 1 cut(s) 1281
PauI GCGCGC 1 cut(s) 1309
PciI ACATGT 2 cut(s) 804, 1322
PctI GAATGC 1 cut(s) 712
PfeI GAWTC 9 cut(s) 139, 144, 182, 248, 843, 944, 980, 986, 1055
PfoI TCCNGGA 1 cut(s) 846
PkrI GCNGC 1 cut(s) 23
PleI GAGTC 3 cut(s) 122, 466, 1216
PpsI GAGTC 3 cut(s) 122, 466, 1216
PscI ACATGT 2 cut(s) 804, 1322
Psp124BI GAGCTC 1 cut(s) 1254
Psp1406I AACGTT 2 cut(s) 790, 1285
Psp6I CCWGG 2 cut(s) 1241, 1255
PspEI GGTNACC 1 cut(s) 779
PspGI CCWGG 2 cut(s) 1241, 1255
PspN4I GGNNCC 1 cut(s) 337
PspPI GGNCC 1 cut(s) 1428
PsrI GAACNNNNNNTAC 2 cut(s) 629, 661
PsuI RGATCY 7 cut(s) 53, 688, 850, 973, 1165, 1271, 1337
PteI GCGCGC 1 cut(s) 1309
RsaI GTAC 3 cut(s) 337, 680, 886
RsaNI GTAC 3 cut(s) 336, 679, 885
RseI CAYNNNNRTG 3 cut(s) 209, 314, 809
SacI GAGCTC 1 cut(s) 1254
SalI GTCGAC 1 cut(s) 527
SaqAI TTAA 6 cut(s) 162, 837, 863, 1197, 1268, 1332
SatI GCNGC 1 cut(s) 22
Sau96I GGNCC 1 cut(s) 1428
SchI GAGTC 3 cut(s) 122, 466, 1216
ScrFI CCNGG 4 cut(s) 95, 848, 1243, 1257
SduI GDGCHC 1 cut(s) 1254
SfaNI GCATC 1 cut(s) 865
SfcI CTRYAG 1 cut(s) 39
SfuI TTCGAA 1 cut(s) 1281
SinI GGWCC 1 cut(s) 1428
SmiMI CAYNNNNRTG 3 cut(s) 209, 314, 809
SmlI CTYRAG 5 cut(s) 49, 275, 1022, 1122, 1385
SmoI CTYRAG 5 cut(s) 49, 275, 1022, 1122, 1385
Sse9I AATT 7 cut(s) 13, 907, 914, 950, 1031, 1062, 1228
SsiI CCGC 2 cut(s) 22, 379
SspMI CTAG 5 cut(s) 18, 179, 245, 752, 1233
SstI GAGCTC 1 cut(s) 1254
StyD4I CCNGG 4 cut(s) 93, 846, 1241, 1255
StyI CCWWGG 1 cut(s) 442
TaaI ACNGT 1 cut(s) 1352
TaiI ACGT 3 cut(s) 793, 1135, 1288
TaqI TCGA 9 cut(s) 63, 142, 231, 327, 528, 880, 920, 964, 1281
TasI AATT 7 cut(s) 13, 907, 914, 950, 1031, 1062, 1228
TatI WGTACW 2 cut(s) 678, 884
TauI GCSGC 1 cut(s) 24
TfiI GAWTC 9 cut(s) 139, 144, 182, 248, 843, 944, 980, 986, 1055
Tru1I TTAA 6 cut(s) 162, 837, 863, 1197, 1268, 1332
Tru9I TTAA 6 cut(s) 162, 837, 863, 1197, 1268, 1332
TscAI CASTG 1 cut(s) 904
TseFI GTSAC 3 cut(s) 779, 823, 1259
Tsp45I GTSAC 3 cut(s) 779, 823, 1259
TspDTI ATGAA 4 cut(s) 508, 1058, 1131, 1408
TspGWI ACGGA 2 cut(s) 662, 887
TspRI CASTG 1 cut(s) 904
VpaK11BI GGWCC 1 cut(s) 1428
XapI RAATTY 3 cut(s) 914, 950, 1031
XceI RCATGY 2 cut(s) 808, 1326
XmiI GTMKAC 1 cut(s) 528
XspI CTAG 5 cut(s) 18, 179, 245, 752, 1233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.